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Glutathione-capped quantum dots for plasma membrane labeling and membrane potential imaging

Nano Research 2019年第6期12卷 1321-1326页

作者： Guangcun Chen Yejun Zhang Zhao Peng dehua huang Chunyan Li Qiangbin Wang CAS Key Laboratory of Nano-Bio Interface Division of Nanobiomedicine and i-LabCAS Center for Excellence in Brain ScienceSuzhou Institute of Nano-Tech and Nano-BionicsChinese Academy of SciencesSuzhou 215123China School of Nano Technology and Nano Bionics University of Science and Technology of ChinaHefei 230026China College of Materials Sciences and Opto-Electronic Technology University of Chinese Academy of SciencesBeijing 100049China

The plasma membrane of cells is a crucial biological membrane that involved in a variety of cellular processes including cell signaling transduction through membrane electrical ***,monitoring membrane electrical activity using fluorescence imaging has attracted numerous attentions for its potential applications in evaluating how the nervous system ***,the development of ideal fluorescent voltage-sensitive probes with both high membrane labeling efficiency and voltage sensitivity is still retain a big ***,glutathionecapped CdSe@ZnS quantum dots (CdSe@ZnS-GSH QDs) with a size of 2.5 nm and an emission peak at 520 nm are synthesized using a facile ligand exchange method for plasma membrane labeling and membrane potential *** as-synthesized CdSe@ZnS-GSH QDs can effectively label cell membrane at neutral pH within 30 min and exhibit excellent optical stability in continuous imaging for up to 60 *** the test concentration up to 200 nM,CdSe@ZnS-GSH QDs show high biocompatibility to cells and do not affect cell proliferation,disturb cell membrane integrity or cause apoptosis and necrosis of ***,a two-component voltage sensor strategy based on fluorescence resonance energy transfer (FRET) between CdSe@ZnS-GSH QDs and the dipicrylamine (DPA) is successfully developed to monitor the membrane potential by the fluorescence of CdSe@ZnS-GSH *** study offers a facile strategy for labeling plasma membrane and monitoring the membrane potential of cells and will hold great potential in the research of signaling within intact neuronal circuits.

关键词： quantum dots membrane labeling membrane potential imaging fluorescence imaging neuron

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Integrating UHPLC-MS/MS quantitative analysis and exogenous purine supplementation to elucidate the antidepressant mechanism of Chaigui granules by regulating purine metabolism

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Journal of Pharmaceutical Analysis 2023年第12期13卷 1562-1576页

作者： Jiajun Chen Tian Li dehua huang Wenxia Gong Junsheng Tian Xiaoxia Gao Xuemei Qin Guanhua Du Yuzhi Zhou Modern Research Center for Traditional Chinese Medicine Shanxi UniversityTaiyuan030006China The Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education Shanxi UniversityTaiyuan030006China The Key Laboratory of Effective Substances Research and Utilization in TCM of Shanxi Province Taiyuan030006China Institute of Materia Medica Chinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing100050China

Chaigui granules(CG)are a compound composed of six herbal medicines with significant antidepressant ***,the antidepressant mechanism of CG remains *** the present study,we attempted to elucidate the antidepressant mechanism of CG by regulating purine metabolism and purinergic ***,the regulatory effect of CG on purine metabolites in the prefrontal cortex(PFC)of chronic unpredictable mild stress(CUMS)rats was analyzed by ultra high-performance liquid chromatography tandem mass spectrometry(UHPLC-MS/MS)targeted quantitative ***,purinergic receptors(P2X7 receptor(P2X7R),A1 receptor(A1R)and A2A receptor(A2AR))and signaling pathways(nod-like receptor protein 3(NLRP3)inflammasome pathway and cyclic adenosine monophosphate(cAMP)-protein kinase A(PKA)pathway)associated with purine metabolism were analyzed by western blotting and enzyme-linked immunosorbent assay(ELISA).Besides,antidepressant mechanism of CG by modulating purine metabolites to activate purinergic receptors and related signaling pathways was dissected by exogenous supplementation of purine metabolites and antagonism of purinergic receptors in *** in vivo study showed that the decrease in xanthine and the increase in four purine nucleosides were closely related to the antidepressant effects of ***,purinergic receptors(P2X7R,A1R and A2AR)and related signaling pathways(NLRP3 inflammasome pathway and cAMP-PKA pathway)were also significantly regulated by *** results of exogenous supplementation of purine metabolites and antagonism of purinergic receptors showed that excessive accumulation of xanthine led to activation of the P2X7R-NLRP3 inflammasome pathway,and the reduction of adenosine and inosine inhibited the A1R-cAMP-PKA pathway,which was significantly ameliorated by ***,CG could promote neuroprotection and ultimately play an antidepressant role by inhibiting the xanthine-P2X7R-NLRP3 inflammasome pathway and activating the adenosine/inosi

关键词： Chaigui granules Traditional Chinese medicine Antidepressant mechanism Purine metabolism Purinergic signaling

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Bio-Mobilization of Potassium from Clay Minerals: Ⅱ. By Ectomycorrhizal Fungi

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Pedosphere 2000年第4期10卷 347-354页

作者： YUAN LING, WANG ZHIHUI, FANG dehua, SHUN HONG and huang JIANGUO (College of Resources and Environment, Southwest Agricultural University, Chongqing 400716 China)

Ectomycorrhizal fungi, including Cenococcum geophilum SIV (Cg SIV), and Pisolithus tinctorius 2144 (Pt 2144), 441 (Pt 441) and XC1 (Pt XC1), were cultured in Pachlewski liquid medium with H2KPO4,KCl- saturated vermiculite and mica as K sources, respectively, to investigate the mechanism of K absorption and mobilization by the fungi. Fungal growth rate, K absorption and mobilization varied significantly among the fungal species. Faster growth and greater K accumulation in Pt XC1 than Pt 2 144, Pt 441 and Cg siv were observed. Ectomycorrhizal fungi depressed HCl-soluble K in minerals after successive extractions by water and NH4OAc. Ratio of the total amount of K, including water-, NH4OAc- and HCI-soluble K, lost from substrates to the K accumulated in fungal colonies was less than 60%. These reveal that the ectomycorrhizal fungi could utilize K in interlayer and structural pools, which are usually unavailable for plants in short period. Large differences in the depletion of K in interlayer and structural pools by fungi were observed at fungal harvest. Taking into account the nutrient absorption by ectomycorrhizal fungi in symbionts and the direct contact between hyphae and soils, the fungi species colonized on the root surfaces seemed to be related to the effectiveness of mycorrhizas to utilize K in soils. Ectomycorrhizal fungi differed in the efflux of protons and oxalate. Pt XC1 was observed to have greatest ability to effuse protons and oxalate among the fungi adopted in the experiment. Furthermore, the higher the concentrations of protons and oxalate in the liquid culture solutions, the larger the depletion of K in interlayer and structural pools in minerals by fungi. Protons could replace interlayer K and chelation of oxalate with Fe and Al in crystal lattice could cause weathering of clay minerals. So, protons and oxalate produced by ectomycorrhizal fungi might play an important role in K mobilization in these two pools.

关键词： ectomycorrhizal fungi K proton and oxalate

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基于半不变量及最大熵的概率谐波潮流算法

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电力系统自动化 2018年第13期42卷 169-174页

作者：曾江蔡东阳黄德华华南理工大学电力学院广东省广州市510641

针对电力系统中谐波分布的不确定性,提出了一种结合半不变量、线性化谐波潮流方程以及最大熵模型的概率谐波潮流算法。首先,根据谐波电流的样本获取高阶矩和半不变量等数字特征,根据电网基础数据构建谐波潮流方程并在基准运行点处线性化... 详细信息

针对电力系统中谐波分布的不确定性,提出了一种结合半不变量、线性化谐波潮流方程以及最大熵模型的概率谐波潮流算法。首先,根据谐波电流的样本获取高阶矩和半不变量等数字特征,根据电网基础数据构建谐波潮流方程并在基准运行点处线性化,计算谐波电压数字特征,进而建立最大熵模型拟合其概率分布。所提方法具有计算量小、编程简单、结果客观准确等优点。最后,使用所提方法在4节点系统上与卷积法比较,以及在IEEE 57节点系统上与蒙特卡洛法比较,均验证了该方法的有效性。

关键词：概率谐波潮流半不变量最大熵原理蒙特卡洛法

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基于InSAR技术的大同市云冈矿区地面沉降监测

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华东地质 2023年第4期44卷 476-484页

作者：黄德华福建省地质测绘院福建福州350011

大同市煤炭资源丰富,但煤矿开采活动造成的地面沉降等地质环境问题一定程度上制约着矿区发展。为监测大同市云冈矿区的地面沉降特征,基于哨兵-1A卫星数据,文章使用星载合成孔径雷达差分干涉测量技术(Differential Interferometric Synth... 详细信息

大同市煤炭资源丰富,但煤矿开采活动造成的地面沉降等地质环境问题一定程度上制约着矿区发展。为监测大同市云冈矿区的地面沉降特征,基于哨兵-1A卫星数据,文章使用星载合成孔径雷达差分干涉测量技术(Differential Interferometric Synthetic Aperture Radar,D-InSAR)技术处理2020年6—7月的2景影像,初步获取矿区沉降区域特征,监测区最大沉降量达4.6 cm/36 d;采用小基线集差分干涉技术(Small Baseline Subset InSAR,SBAS-InSAR)处理2019年1月—2020年12月的24景影像,得到监测区部分沉陷区域的长时间序列形变量,该形变量与2020年1月—2020年9月的同地区25景影像数据永久散射体合成孔径雷达干涉测量(Persistent Scattered Interferometric Synthetic Aperture Radar,PS-InSAR)监测形变空间分布一致,形变趋势吻合。综合以上3种InSAR监测结果表明,监测区内存在多达12个明显快速沉降区,均分布于矿区内,其中最大沉降速率达180 mm/y,2019—2020年最大累积沉降量为333 mm,东南部城市区域没有明显形变迹象。研究结果为矿区沉降监测及合理开采提供科学依据。

关键词： D-InSAR SBAS-InSAR 时间序列 PS-InSAR 矿区沉降监测大同市云冈矿区

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基于分割网络的海滩泡沫浮球遥感影像自动识别应用研究

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福建地质 2023年第4期42卷 296-304页

作者：黄德华福建省地质测绘院福州350011

通过人工目视解译福建沿海23个岸段无人机影像,获取到足够的泡沫浮球样本数据。对4个语义分割网络模型进行训练,对比现有3种主流结构(CNN、CNN+Transformer、Transformer)在海滩塑料泡沫浮球分割任务上的识别效果。实验结果表明:Transu... 详细信息

通过人工目视解译福建沿海23个岸段无人机影像,获取到足够的泡沫浮球样本数据。对4个语义分割网络模型进行训练,对比现有3种主流结构(CNN、CNN+Transformer、Transformer)在海滩塑料泡沫浮球分割任务上的识别效果。实验结果表明:Transunet网络与U-net、DeepLabv3+、Swin-Unet对比,对于不同密集程度、不同大小的海滩塑料泡沫浮球有更高的识别精度。对网络结构而言,CNN+Transformer结构的语义分割网络具有更高的识别精度,可以更好地应对复杂的海滩环境,在实际应用中具有很大的潜力。对识别场景而言,Transunet在所有场景中都表现最佳。对比现有不同结构语义分割网络,为海滩塑料泡沫浮球自动识别提供了一种可行的解决方案。

关键词：海滩塑料垃圾泡沫浮球无人机遥感语义分割网络福建沿海

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福建山区机载LiDAR微地貌识别最优点密度研究

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测绘与空间地理信息 2023年第10期46卷 132-136页

作者：黄德华福建省地质测绘院福建福州350011

针对地形复杂、植被覆盖度高的隐蔽性地质灾害的识别,激光雷达技术(light detection and ranging,LiDAR)较传统手段具有非接触、高效率、精准判识等优点。本文采用机载LiDAR、有人机航拍等手段对福建省平溪镇—下党乡潜在高位隐蔽性地... 详细信息

针对地形复杂、植被覆盖度高的隐蔽性地质灾害的识别,激光雷达技术(light detection and ranging,LiDAR)较传统手段具有非接触、高效率、精准判识等优点。本文采用机载LiDAR、有人机航拍等手段对福建省平溪镇—下党乡潜在高位隐蔽性地质灾害隐患区进行数据采集及地质灾害隐患早期识别工作。首先,采用整体滤波为主,人工精细化分类为辅的方法进行点云去噪滤波处理;其次,利用随机算法抽稀高密度点云数据;最后,分析不同植被覆盖度下,不同点云密度对微地貌识别效果。

关键词：机载LiDAR 灾害隐患早期识别微地貌最优点密度

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复合界址线的自动规范化处理方法与算法实现

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测绘科学 2022年第6期47卷 168-173页

作者：许承权张帆黄德华吴永红闽江学院地理与海洋学院福州350108 福建省测绘院福州350100 福建省地质测绘院福州350100

针对带圆弧或带孤岛的复合界址线在数据格式转换过程中可能出现偏差、错位、自相交等错误,人工修改工作量巨大的问题,该文提出了一种新的复合界址线自动规范化算法。通过精度反推圆弧节点数量,采用极坐标法的等角切分算法计算出圆弧上... 详细信息

针对带圆弧或带孤岛的复合界址线在数据格式转换过程中可能出现偏差、错位、自相交等错误,人工修改工作量巨大的问题,该文提出了一种新的复合界址线自动规范化算法。通过精度反推圆弧节点数量,采用极坐标法的等角切分算法计算出圆弧上任意位置的坐标,实现带圆弧复合界址线的自动规范化;采用用户交互的方式在带孤岛界址线的连接线上增加节点,解决界址线的线段自相交问题;再计算界址线各节点的方位角,自动查找左上角凸点,并转换界址线起点和方向,实现复合界址线的规范化。实验结果表明:该文所提算法较好地解决了复合界址线数据格式转换过程中产生的问题,避免人工转换的麻烦,可以快速实现界址线自动规范化,为后续土地勘测定界报告生成、界址线数据格式转换等提供方便。

关键词：土地勘测定界复合界址线等角切分算法孤岛自动规范化

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