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Interaction between human serum albumin and cholesterol-grafted polyglutamate as the potential carriers of protein drugs

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Acta Pharmaceutica Sinica B 2019年第1期9卷 186-193页

作者： Xiangxue Lyu Qiufen Zhang dehai liang Yanbin Huang Key Laboratory of Advanced Materials (MOE) Department of Chemical Engineering Tsinghua University College of Chemistry & Molecular Engineering Peking University

Currently there is no successful platform technology for the sustained release of protein *** seems inevitable to specifically develop new materials for such purpose, and hence the understanding of protein–material interactions is highly desirable. In this study, we synthesized cholesterol-grafted polyglutamate(PGA-g-Chol) as a hydrophobically-modified polypeptide, and thoroughly characterized its interaction with a model protein(human serum albumin) in the aqueous solution by using circular dichroism, fluorescence methods, and light scattering. With the protein concentration fixed at 5 μmol/L,adding PGA-g-Chol polymers into the solution resulted in continuous blue shift of the protein fluorescence(from 339 to 332 nm), until the polymer molar concentration reached the same value as the protein. In contrast, the un-modified polyglutamate polymers apparently neither affected the protein microenvironment nor formed aggregates. Based on the experimental data, we proposed a physical picture for such protein–polymer systems, where the polymer first bind with the protein in a 1:1 molar ratio via a fraction of their hydrophobic pendant cholesterol resides along the polymer chain. In this protein/polymer complex, there are excess unbound cholesterol residues. As the polymer concentration increases, the polymers form multi-polymer aggregates around 200 nm in diameter via the same hydrophobic cholesterol residues. The protein/polymer complex also participate in the aggregation via their excess cholesterol residues, and consequently the proteins are encapsulated into the nanoparticles. The encapsulation was also found to increase the thermal stability of the model protein.

关键词： Protein drug delivery Polypeptide Self-assemble Human serum albumin Interaction Carrier

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Encapsulation of RNA by negatively charged human serum albumin via physical interactions

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Science China Chemistry 2017年第1期60卷 130-135页

作者： Hao Wen Yudan Yin Chao Huang Wei Pan dehai liang Beijing National Laboratory for Molecular Sciences and the Key Laboratory of Polymer Chemistry and Physics of Ministry of Education College of Chemistry and Molecular Engineering Peking University Beijing 100871 China

Human serum albumin(HSA)is the most abundant plasma protein and has an inherent ability to target tumor *** is an excellent candidate for drug ***,HSA cannot form complex with DNA or RNA,because it is negatively charged under physiological *** this work,we reported a simple method to prepare HSA/RNA nanoparticles mainly by physical ***,the solution p H is adjusted to 4.0,under which condition HSA is positively *** forms complex with RNA via electrostatic *** solution is then heated at 75 oC for 15 min to stabilize the structure and the size of the formed *** HSA/RNA nanoparticle prepared by this method has a diameter about 110 nm and a narrow *** is also stable for days under physiological *** essays demonstrate that these particles exhibit a high cellular uptake efficiency and non-toxicity to He La cells.

关键词： biopolymers complexation electrostatic interaction nanoparticle drug delivery

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Shear-responsive peptide/siRNA complexes as lung-targeting gene vectors

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Chinese Chemical Letters 2021年第5期32卷 1731-1736页

作者： Dongxiao Yin Mengjie Zhang Jiaxin Chen Yuanyu Huang dehai liang Beijing National Laboratory for Molecular Sciences and the Key Laboratory of Polymer Chemistry and Physics of Ministry of Education College of Chemistry and Molecular EngineeringPeking UniversityBeijing 100871China School of Life Science Advanced Research Institute of Multidisciplinary ScienceInstitute of Engineering MedicineKey Laboratory of Molecular Medicine and BiotherapyBeijing Institute of TechnologyBeijing 100081China

Particles administrated intravenously will pass through the pulmonary capillary network before being distributed to the ***,fabrication of vectors sensitive to blood shear and active with blood components should be a practical approach to develop lung-targe ting gene carriers self-regulated by circulatory *** this work,we designed a series of cationic peptides with the same charge density but varying hydrophobicity and capacity to form hydrogen bonds,and investigated their ability to form co mplexes with siRNA,the behaviours of peptide/siRNA complexes in the presence of serum under shear,and the lung-targeting efficacy of the complexes regulated by *** hydrophobic interaction co ntrols the complexation between peptide and siRNA,while the hydrogen bonds are responsible for the binding of peptides to the serum components in *** vivo tests show that all the peptide/siRNA complexes can accumulate in ***,only the complexes that exhibit weak interaction with serum components and can be broken down by shear avoid the inflammation and death caused by pulmonary ***,the peptide with strong hydrophobicity can retain siRNA in lung without early release of the *** study provides a step toward the development of adaptive gene carriers under the regulation of circulatory system.

关键词： Peptide Gene delivery Shear Polyelectrolyte complexes

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Effects of chain length and hydrophobicity/charge ratio of AMP on its antimicrobial activity

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Science China Chemistry 2017年第3期60卷 385-395页

作者： Qiuhong Yu Zhengxi Zhang Jianbo Sun Yuqiong Xia Quan Du dehai liang Beijing National Laboratory for Molecular Sciences and the Key Laboratory of Polymer Chemistry and Physics of Ministry of Education State Key Laboratory of Natural and Biomimetic DrugsCollege of Chemistry and Molecular EngineeringSchool of Pharmaceutical SciencesPeking University School of Life Sciences and Technology Xidian University

Using two series of de novo designed antimicrobial peptides,we studied the effects of peptide length and hydrophobicity/charge(H/C) ratio on the antimicrobial *** these peptides,a correlation was established between their antimicrobial efficacy and the leakage,aggregation,and fusion activities on artificial *** results showed that peptides with an H/C ratio of 1.3,and a length of about half of the membrane thickness caused most potent membrane leakage,and negligible membrane aggregation and *** addition,such peptide also exhibited the highest antimicrobial *** of the hydrophobic and electrostatic interactions showed that the strength,the order and the position of these interactions determined the activities of the peptides on the artificial membrane and thus the antimicrobial *** analyses on the tilt angle of the peptides on the membrane surface indicated that the peptides distorted the membrane in a dynamic mode,instead of being fixed in the membrane at a constant angle.

关键词： antimicrobial peptides peptide length hydrophobicity/charge ratio

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Complexation of DNA with cationic surfactants as studied by small-angle X-ray scattering

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Science China Chemistry 2014年第12期57卷 1738-1745页

作者： NIU Lin YAN JingJing YANG XuYan BURGER Christian RONG LiXia HSIAO Benjamin liang dehai Beijing National Laboratory for Molecular Sciences the Key Laboratory of Polymer Chemistry and Physics of the Ministry of Education Department of Polymer Science and Engineering College of Chemistry and Molecular Engineering Peking University Department of Chemistry State University of New York at Stony Brook

The phase behaviors of the complex formed by didodecyldimethylammonium bromide（DDAB）and cetyltrimethylammonium bromide（CTAB）interacting with three different types of DNAs,salmon testes DNA（~2000 bp）,21-bp double-stranded oligonucleotides（oligo-ds DNA）,and 21-nt single-stranded oligonucleotides（oligo-ss DNA）were studied by synchrotron small-angle X-ray *** was found that the DNA length and flexibility,together with the positive/negative charge ratio,determined the final *** higher charge ratios,the DNA length exhibited negligible *** oligo-ds DNA and salmon DNA formed inverted hexagonal packing of cylinders with CTAB,as well as bilayered lamella with ***,at lower charge ratios,oligo-ds DNA formed a distorted hexagonal phase with CTAB and a new structure with DDAB,which was different from the behaviors of salmon *** flexible oligo-ss DNA formed rich structures that were subject to environmental *** study also indicated that the structures of the complex formed by oligo-ss DNA took much longer to mature than the structures formed by oligo-ds *** attributed this result to the conformational adjustment of oligo-ss DNA in the complex.

关键词： phase behavior DNA surfactant synchrotron small-angle X-ray scattering kinetics

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High-performance CsPbBr_(3)@Cs_(4)PbBr_(6)/SiO_(2) nanocrystals via double coating layers for white light emission and visible light communication

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eScience 2022年第6期2卷 646-654页

作者： Xianwen Li Wen Ma dehai liang Wensi Cai Shuangyi Zhao Zhigang Zang Key Laboratory of Optoelectronic Technology&Systems(Ministry of Education) Chongqing UniversityChongqing 400044China

Owing to their outstanding optoelectronic properties,all-inorganic CsPbBr_(3) perovskite nanocrystals(NCs)are regarded as excellent materials for various optoelectronic ***,their practical applications are limited by poor stability against water,heat,and polar ***,we propose a facile synthesis strategy for CsPbBr_(3)@Cs_(4)PbBr_(6) NCs via tetraoctylammonium bromide ligand induction at room *** resulting CsPbBr_(3)@Cs_(4)PbBr_(6) NCs show a high photoluminescence quantum yield of 94%.In order to prevent Cs4PbBr6 from being converted back to CsPbBr_(3) NCs when exposed to water,a second coating layer of SiO2 is formed on the surface of the CsPbBr_(3)@Cs_(4)PbBr_(6) NCs by the facile hydrolysis of *** resulting CsPbBr_(3)@Cs_(4)PbBr_(6)/SiO_(2) NCs with their double coating structure have outstanding stability against not only a polar solvent(ethanol)but also water and *** as-prepared CsPbBr_(3)@Cs_(4)PbBr_(6)/SiO_(2) NCs serve as green emitters in efficient white light-emitting diodes(WLEDs)with a high color rendering index(CRI)of 91 and a high power efficiency 59.87 lm W−***,the use of these WLEDs in visible light communication(VLC)results in a maximum rate of 44.53 Mbps,suggesting the great potential of the reported methods and materials for solid-state illumination and VLC.

关键词： CsPbBr_(3)@Cs_(4)PbBr_(6)/SiO_(2)NCs Stability White light emission Visible light communication

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Zn（Ⅱ） can mediate self-association of the extracellular C-terminal domain of CD147

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Protein & Cell 2018年第3期9卷 310-315页

作者： Shujuan Jin Pengfei Ding Pengxiang Chu Hongwei Li Jianbo Sun dehai liang Fei Song Bin Xia Beijing Nuclear Magnetic Resonance Center Peking University Beijing 100871 China College of Chemistry and Molecular Engineering Peking University Beijing 100871 China School of Life Sciences Peking University Beijing 100871 China Cell Engineering Research Center and Department of Cell Biology State Key Laboratory of Cancer Biology The Fourth Military Medical University Xi'an 710032 China

CD147 （cluster of differentiation 147）, also known as basigin or extracellular matrix metalloproteinase inducer （EMMPRI N）, is a cell-surface type I transmembrane glycoprotein expressed at different levels in various cells and tissues, especially at high levels in tumor cells （Grass and Toole, 2015）. CD147 plays important roles in multiple physiological processes, such as spermatogenesis, neural network formation, T-cell activation and in the progression of several diseases including tumor metastasis rheumatoid arthritis （RA） atherosclerosis malaria and HIV infection （Muramatsu, 2016）.

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Near-unity quantum yield in zero-dimensional lead-free manganese-based halides for flexible X-ray imaging with high spatial resolution

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eScience 2023年第2期3卷 103-111页

作者： Wen Ma dehai liang Qingkai Qian Qionghua Mo Shuangyi Zhao Wensi Cai Jiangzhao Chen Zhigang Zang Key Laboratory of Optoelectronic Technology&Systems(Ministry of Education) Chongqing UniversityChongqing400044China

Low-dimensional luminescent lead-free metal halides have received substantial attention due to their unique optoelectronic *** them,zero-dimensional(0D)manganese(II)-based metal halides with negligible self-absorption have emerged as potential candidates in X-ray ***,we for the first time report a novel lead-free(TBA)_(2)MnBr_(4) single crystal synthesized via a facile solvent evaporation *** this crystal,[MnBr_(4)]^(2-)units are isolated by large TBA^(+)organic cations,resulting in a unique 0D *** prepared manganese-based crystals exhibit a bright-green emission centered at 512 nm with a high photoluminescence quantum yield(PLQY)of 93.76%at room temperature,originating from the ^(4)T_(1)–^(6)A_(1) transition of Mn^(2+).Apart from their outstanding optical performance,the crystals also show excellent stability and can maintain 94.4%of the initial PLQY even after being stored in air for 90 ***(TBA)_(2)MnBr4 films prepared as X-ray imaging scintillators exhibit a low detection limit of 63.3 nGyair/s,a high light yield of 68000 ph/MeV,and a high spatial resolution of 15.4 lp/***,this work not only enriches the family of lead-free metal halides but also expands the application of manganese(II)-based halides in flexible X-ray scintillators.

关键词： Manganese(II)-based metal halides Organic-inorganic hybrids Single crystals Flexible X-ray imaging

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Luminescence regulation of Sb^(3+)in 0D hybrid metal halides by hydrogen bond network for optical anti-counterfeiting

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Opto-Electronic Advances 2024年第3期7卷 15-25页

作者： dehai liang Saif M.H.Qaid Xin Yang Shuangyi Zhao Binbin Luo Wensi Cai Qingkai Qian Zhigang Zang Key Laboratory of Optoelectronic Technology&Systems(Ministry of Education) Chongqing UniversityChongqing 400044China Department of Physics&Astronomy College of SciencesKing Saud UniversityP.O.Box 2455Riyadh 11451Saudi Arabia Department of Chemistry and Chemical Engineering Key Laboratory for Preparation and Application of Ordered Structural Materials of Guangdong ProvinceShantou UniversityShantou 515063China Department of Oil Army Logistics Academy of PLAChongqing 401311China

The Sb^(3+) doping strategy has been proven to be an effective way to regulate the band gap and improve the photophysical properties of organic-inorganic hybrid metal halides(OIHMHs).However,the emission of Sb^(3+) ions in OIHMHs is primarily confined to the low energy region,resulting in yellow or red *** date,there are few reports about green emission of Sb^(3+)-doped ***,we present a novel approach for regulating the luminescence of Sb^(3+) ions in 0D C_(10)H_(2)_(2)N_(6)InCl_(7)·H_(2)O via hydrogen bond network,in which water molecules act as agents for hydrogen ***^(3+)-doped C_(10)H_(2)2N_(6)InCl_(7)·H_(2)O shows a broadband green emission peaking at 540 nm and a high photoluminescence quantum yield(PLQY)of 80%.It is found that the intense green emission stems from the radiative recombination of the self-trapped excitons(STEs).Upon removal of water molecules with heat,C_(10)H_(2)_(2)N_(6)In_(1-x)Sb_(x)Cl_(7) generates yellow emis-sion,attributed to the breaking of the hydrogen bond network and large structural distortions of excited *** water molecules are adsorbed by C_(10)H_(2)_(2)N_(6)In_(1-x)Sb_(x)Cl_(7),it can subsequently emit green *** water-induced reversible emission switching is successfully used for optical security and information *** findings expand the under-standing of how the local coordination structure influences the photophysical mechanism in Sb^(3+)-doped metal halides and provide a novel method to control the STEs emission.

关键词： indium-based halides Sb^(3+)doping hydrogen bonding network optical anti-counterfeiting

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熔铸锆刚玉制品的特征及质量判定

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玻璃与搪瓷 2016年第4期44卷 28-33页

作者：梁德海中国中轻国际工程有限公司北京100026

熔铸锆刚玉制品中各部位化学成分、矿相组成及体积密度不均匀性是它区别于烧成和不烧成致密定型耐火制品的主要特征;在玻璃熔窑上使用后的原砖残存部分的矿相组成和化学成分已有变化,因此每一批熔铸锆刚玉制品在使用前必须严格按照出厂... 详细信息

熔铸锆刚玉制品中各部位化学成分、矿相组成及体积密度不均匀性是它区别于烧成和不烧成致密定型耐火制品的主要特征;在玻璃熔窑上使用后的原砖残存部分的矿相组成和化学成分已有变化,因此每一批熔铸锆刚玉制品在使用前必须严格按照出厂检验、判定检验及落地检验的检验程序,完成产品是否合格及能否使用的判定工作。

关键词：熔铸锆刚玉耐火材料化学成分矿相组成体积密度检验

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