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Integration of chemoselective ligation with enzymespecific catalysis： Saccharic colorimetric analysis using aminooxy/hydrazine-functionalized gold nanoparticles

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Nano Research 2015年第12期8卷 3853-3863页

作者： Juan Zhang Jun Lv Xiaonan Wang defeng li Zhaoxia Wang Genxi li Laboratory of Biosensing Technology School of Life Sciences Shanghai University Shanghai 200444 China Shanghai Key Laboratory of Bio-Energy Crops Shanghai University Shanghai 200444 China Department of Oncology The Second Affiliated Hospital of Nanjing Medical University Nanjing 210011 China State Key Laboratory of Pharmaceutical Biotechnology Department of Biochemistry Nanjing University Nanjing 210093 China

Here we developed a saccharic colorimetric method based on the combination of chemoselective ligation and enzyme-specific catalysis using aminooxy/ hydrazine-functionalized gold nanoparticles （AO/AuNPs or H/AuNPs）. In the detection of galactose （Gal）, galactohexodialdose （GHDA）, the galactose oxidase （GalOx）-catalyzed product, has an aldehyde group, which allows it to chemoselectively react with an aminooxy or hydrazine group at the outer layer of AO/AuNPs or H/AuNPs by oxime/hydrazone click chemistry to form oxime or hydrozone. Consequent134 through the specific recognition of 1,4-phenylenediboronic acid （PDBA） on cis-diols, GHDA, which contains two pairs of hydroxyls in the cis form, can bind not only with AO/AuNPs or H/AuNPs, but also with PDBA to form boronate diester, thereby triggering the aggregation of AuNPs and causing the corresponding color change. As GalOx catalyzed specific substrates, the amount of Gal correlated with the production of GHDA and the extent of AuNPs aggregation, thus allowing a simple and easily operatable colorimetric method for Gal detection to be developed. Under the optimized experimental conditions, the ratios of absorbance at a wavelength of 617 nm to that at 536 nm vary linearly with the logarithmic values of Gal concentrations within a wide range of 500 nM to 5 mM. Moreover, this colorimetric method shows anti-interference capability and high sensitivity with a detection limit of 21 nM. Thus, a universal platform for accurate and specific colorimetric analysis can be established through the integration of chemoselective ligation with enzyme specific catalysis.

关键词： oxime/hydrazone ligation enzyme catalysis aminooxy/hydrazine-functionalized gold nanoparticles

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In Situ Preparation and Analysis of Bimetal Co?doped Mesoporous Graphitic Carbon Nitride with Enhanced Photocatalytic Activity

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Nano-Micro Letters 2019年第1期11卷 175-190页

作者： Wanbao Wu Zhaohui Ruan Junzhuo li Yudong li Yanqiu Jiang Xianzhu Xu defeng li Yuan Yuan Kaifeng lin School of Chemistry and Chemical Engineering Harbin Institute of Technology Key Laboratory of Aerospace Thermophysics Ministry of Industry and Information TechnologyHarbin Institute of Technology

A novel photocatalyst of mesoporous graphitic carbon nitride(g-C_3N_4) co-doped with Co and Mo(Co/Mo-MCN) has been one-pot synthesized via a simple template-free method; cobalt chloride and molybdenum disulfide were used as the Co and Mo sources, respectively. The characterization results evidently indicate that molybdenum disulfide functions as Mo sources to incorporate Mo atoms in the framework of g-C_3N_4 and as a catalyst for promoting the decomposition of g-C_3N_4, resulting in the creation of mesopores. The obtained Co/Mo-MCN exhibited a significant enhancement of the photocatalytic activity in H_2 evolution(8.6 times) and Rhodamine B degradation(10.1 times) under visible light irradiation compared to pristine g-C_3N_4. Furthermore, density functional theory calculations were applied to further understand the photocatalytic enhancement mechanism of the optical absorption properties at the atomic level after Co-or Mo-doping. Finite-di erence time-domain simulations were performed to evaluate the e ect of the mesopore structures on the light absorption capability. The results revealed that both the bimetal doping and the mesoporous architectures resulted in an enhanced optical absorption; this phenomenon was considered to have played a critical role in the improvement in the photocatalytic performance of Co/Mo-MCN.

关键词： Co-doped g-C3N4 Porous g-C3N4 Photocatalysis Optical simulation light absorption intensity

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Easily Separated and Recyclable Amino-functionalized Porous SiO2 Beads with 3D Continuous Meso/Macropore Channels

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Chemical Research in Chinese Universities 2018年第1期34卷 13-18页

作者： WANG Qi YANG Xiaohui JIANG Yanqiu HUO Hang li defeng liN Kaifeng XU Xianzhu MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage School of Chemistry and Chemical Engineering Harbin lnstimte of Technology Harbin 150090 P. R. China Key Laboratory of Advanced Energy Materials Chemistry Ministry of Education Nankai University Tianjin 300071 P. R. China

Amino-fimctionalized porous SiO2 beads with a diameter of 200--800 μm（PSB-NH2） have been success- fully synthesized by grafting 3-ammopropyl-triethoxysilane onto meso/macroporous silica beads（PSB）, in which the PSB was prepared by hydrothermal synthetic method with a porous hard template anion-exchange resin. The as-prepared materials were characterized by means of nitrogen sorption and transmission electron micrographs（TEM）, showing the presence of 3D interconnected and continuous large mesopores and macropores inside. The beads were used to catalyze Knoevenagel condensation and proved to be highly active and selective due to the high accessibility of the reactants to the amino groups via the continuous 3D meso/macopores. Notably, such material in bead format facilitates the extremely straightforward separation from reaction solution without any centrifugation or filtration. Moreover, PSB-NH2 proved to be a stable catalyst via leaching experiment test, and can be easily recovered and reused without significant loss of activity in successive catalytic cycles.

关键词： Amino group, Porous SiO2 bead, Continuous 3D meso/macropore Knoevenagel condensation Straightforward separation

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Mapping the small-world properties of brain networks in Chinese to English simultaneous interpreting by using functional near-infrared spectroscopy

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Journal of Innovative Optical Health Sciences 2018年第3期11卷 8-19页

作者： Xiaohong lin Victoria Lai Cheng Lei defeng li Zhishan Hu Yutao Xiang Zhen Yuan Faculty of Health Sciences University of Macao Macao SARP.R.China Department of English Faculty of Arts and Humanities University of Macao Macao SARP.R.China Centre for Studies of Translation Interpreting and Cognition University of Macao Macao

The aim of this study is to examine the small-world properties of functional brain networks inChinese to English simultaneous interpreting(SI)using functional near-infrared spectroscopy(INIRS),In particular,the fNIRS neuroimaging combined with complex network analysis wasperformed to extract the features of functional brain networks underling three translationstrategies associated with Chinese to English SI:"transcoding"that takes the"shortcut"linkingtranslation equivalents between Chinese and the English,code-mixing"that basically does notinvolve blingual procesing,and"transphrasingn that takes the long route"involving amonolingual processing of meaning in Chinese and then another monolingual processing ofmeaning in *** results demonstrated that the small-world net work topology was able todistinguish well bet ween the transcoding,code-mixing and transphrasing strategies related toChinese to English SI.

关键词： fNIRS translation simultaneous interpreting small-world brain net work

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Chinese, Japanese, Korean Women’s Conference Held in Jinan, Shandong Province

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Voice of Friendship 2006年第1期 22-23页

作者： li defeng

On October 17, 2005, the Chinese, Japanese, Korean Women’s Conference jointly spon- sored by the Foreign Affairs Office of the Shandong Provincial People’s Government, the Shandong Provincial Women’s Federation and... 详细信息

关键词： Japanese Jinan Korean Women Chinese Shandong exhib

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Origin, dissemination and entry of the pandemic Zika viruses

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Science Bulletin 2016年第10期61卷 749-751页

作者： Huimin Zhang Swaminath Srinivas defeng li Youjun Feng Department of Medical Microbiology and Parasitology Zhejiang University School of Medicine Hangzhou 310058 China Institute for Genomic Biology University of Illinois UrbanaIL 61801 USA Department of Biochemistry University of Illinois UrbanaIL 61801 USA Institute of Biophysics Chinese Academy of SciencesBeijing 100101 China

Zika virus （ZIKV） is a previously-neglected, but newly-emerging zoonotic pathogen that has swept most of the western hemisphere. The unexpected attention aroused worldwide lies in the explosive spread of ZIKV to Brazil and USA in 2015 and its rare correlation to micro- cephaly in newborns, raising a serious challenge to public health. We show the origin and fast dissemination of these epidemic ZIKA isolates. Phylogenetic analyses revealed that all the newly-emerging ZIKV isolates belong to the Asian-American lineage. Structural analyses of the ZIKV E surface protein suggested that it might adopt a similar entry mechanism to that of other Flavi-viruses like Dengue Virus and West Nile Virus.

关键词： Zika virus Flavivirus DisseminationEntry Origin

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翻译认知过程研究的发展趋势——李德凤教授访谈录

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东方翻译 2020年第1期 71-75页

作者：徐然李德凤外交学院英语系澳门大学人文学院翻译传译认知研究中心

翻译认知过程研究近年来发展迅猛。本文是笔者对翻译认知过程研究学者李德凤教授的访谈要点记录,内容涵盖翻译认知过程研究的定位和发展趋势,翻译认知过程研究和语料库研究的关系,技术变革给翻译认知过程研究带来的影响等话题。李德凤... 详细信息

翻译认知过程研究近年来发展迅猛。本文是笔者对翻译认知过程研究学者李德凤教授的访谈要点记录,内容涵盖翻译认知过程研究的定位和发展趋势,翻译认知过程研究和语料库研究的关系,技术变革给翻译认知过程研究带来的影响等话题。李德凤教授认为,翻译过程研究目前是非常活跃的研究领域,包括认知语言学理论、神经科学理论、认知科学理论、心理语言学理论在内的诸多理论都可以和翻译过程研究相结合。翻译认知过程研究可将过程研究与产品研究相结合,是翻译学未来发展强有力的增长点,可带动整个翻译学科的研究方法、跨学科性的提升和学科内部的融合。他还介绍了自己所在的澳门大学翻译传译认知研究中心的研究思路,并为青年学者提出了一些建议。

关键词：翻译认知过程翻译过程研究认知语言学理论翻译学科澳门大学跨学科性心理语言学理论神经科学

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Structural and functional study of MCP2201 from CNB-1

Structural and functional study of MCP2201 from CNB-1

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第五届中国结构生物学学术讨论会

作者： Yuan Hong Yonglin Hu defeng li Dacheng Wang National Laboratory of Biomacromolecules Institute of Biophysics Chinese Academy of Sciences

Chemotaxis is an important physiological response to chemical compounds and enables bacteria to be more competitive for nutrition and avoidance of harsh conditions. The study of chemoreceptors, also known as methyl-accepting chemotaxis proteins（MCPs）, provides us the chance of understanding the chemotaxis mechanism and the transmembrane signaling mechanism. A piston model for the chemoreceptor transmembrane signaling has been proposed for at least ten years and not yet been demonstrated by the structures of ligand-free and-bound states. Here we reported the crystal structures of attractant-free and-bound ligand-binding-domain（LBD2201） of MCP2201, a recently identified chemoreceptor from Comamonas testosteroni CNB-1. The comparison of the protomer structure in attractant-free and-bound forms indicated a loop-tohelix transition in the N-terminus of helix α2 upon attractant binding, which exactly illustrated the structural basis of the piston model for the chemoreceptor transmembrane signaling. Interestingly, the attractant-free and-bound LBD2201 exist as a dimer used to be observed for other chemoreceptors and a trimer never observed previously, respectively, implying that an associationdisassociation model should be involved in the transmembrane signaling of chemoreceptors. Mutation based crosslinking experiments on living bacteria stimulated by the attractant captured expected trimer, implying which exists exactly in vivo. Motility plate assays showed mutations that are designed to disrupt trimer interface or interactions between attractant molecule and ligand binding cavity lost their chemotaxis activity toward the attractant. This suggested chemotaxis activation of MCP2201 depends on a dimer-to-trimer transition upon the attractant binding. In a summary, our study provided a new insight into the activating mechanism of MCPs and the transmembrane signaling.

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古代蒙古族史学史论纲

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史学史研究 2015年第4期 69-78页

作者：李德锋内蒙古大学历史与旅游文化学院内蒙古呼和浩特010021

蒙古族有着悠久的历史和文化,其史学于古代的发展大致经历了五个阶段。13世纪初以前为萌芽阶段,主要内容是早期的传说和神话,这些神话和传说说明了蒙古族历史意识的萌发,同时也为其后的产生和发展奠定了基础。大蒙古国时期是蒙古族史学... 详细信息

蒙古族有着悠久的历史和文化,其史学于古代的发展大致经历了五个阶段。13世纪初以前为萌芽阶段,主要内容是早期的传说和神话,这些神话和传说说明了蒙古族历史意识的萌发,同时也为其后的产生和发展奠定了基础。大蒙古国时期是蒙古族史学正式建立和初步发展的阶段,产生了蒙古族典型的民族史学著作《蒙古秘史》。元代是蒙古族史学的一个发展高峰期,蒙古民族在元朝的官修史学活动中发挥着非常重要的作用。北元——蒙古时期史学主要特征是黄教与史学的结合,而黄教介入蒙古族史学,也使得其面临着挫折与发展并存的局面。清代蒙古族史学发展进入了总结与嬗变时期,其中既有对黄教与史学结合方面的总结性发展,也有对这一传统的突破;在史籍体裁、语言文字等方面也充分借鉴和发展了古代中国其他兄弟民族的史学和文化成果,同时在西方列强入侵的时代背景下,蒙古族史学也开始了走向近代的历程。整体看来,蒙古族史学表现出较为宽广的研究前景和现实意义。

关键词：蒙古族史学史《蒙古秘史》《黄金史纲》《蒙古源流》

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明唐顺之《左氏始末》刍议

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南开学报（哲学社会科学版） 2021年第2期 130-140页

作者：李德锋内蒙古大学历史与旅游文化学院内蒙古呼和浩特010021

唐顺之《左氏始末》改《左传》编年体为纪事本末体,突出了纪事原则,但标目也存在以人分类的现象,甚至是名实不符的情况。《左氏始末》以《左传》作为基本史料来源,还充分参考了《国语》《史记》和后出的其他文献,是对这些文献的综合运... 详细信息

唐顺之《左氏始末》改《左传》编年体为纪事本末体,突出了纪事原则,但标目也存在以人分类的现象,甚至是名实不符的情况。《左氏始末》以《左传》作为基本史料来源,还充分参考了《国语》《史记》和后出的其他文献,是对这些文献的综合运用。这些体现了传统体裁由以纪年、记人向纪事转变过程中的变化,反映了唐顺之本人对春秋时代"无义"的整体历史评价,突出了在传统"以史证经"的思路下,对史学价值的特别认可。

关键词：唐顺之《左氏始末》类例史源编纂意义

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