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International Journal of Ophthalmology(English edition) 2017年第12期10卷 1798-1804页

作者： Min Wu de-xuan kuang Ya-Qi Huang Yu-Run Miao Xiao-Cheng Liu Jie-Jie Dai department of Ophthalmology Yunnan Key Laboratory for prevention and treatment of eye diseases Yunnan Innovation Team for Cataract and Ocular fundus Disease(2017HC010)Yunnan Eye Institutethe 2^nd People’s Hospital of Yunnan Province(Yunnan Eye Hospitalthe 4 Afiliated Hospital of Kunming Medical University)Kunming 650021Yunnan ProvinceChina Center of Tree Shrew Germ plasm Resources Institute of Medical BiologyChinese Academy of Medical Science and Peking Union Medical CollegeYunnan Key Laboratory of Vaccine Research and Development on Severe Infectious DiseasesYunnan Innovation Team of Standardization andApplication Research in Tree ShrewKunming 650118Yunnan ProvinceChina

AIM：To determine the impact of age on the morphology of endothelial cells and central corneal thickness（CCT） in Chinese tree ***：One-hundred and twenty eyes of 60 healthy Chinese tree shrews were *** on age,the tree shrews were divided into four *** general anesthesia,the images of endothelium were acquired using non-contact specular microscope Topcon 3000 *** parameters of corneal endothelial cells were measured by built-in software,including CCT,endothelial cell density（ECD）,percent hexagonality（HG%）,coeffi cient of variability（CV）,size of minimal cell（Smin）,size of maximal cell（Smax）,average cells size（Savg） and size standard deviation（Ssd）.Data were analyzed using STATA *** differences of eight parameters among groups and correlations with age were ***：In all studied animals,the average CCT was 2 4 9.6 ± 2 .29 μ m（202-301 μm）,ECD was 308 0.72 ± 460.76 cells/mm^2（1239.6-4047.6 cells/mm^2） and CV was 29.10±7.60（13.6-54.6）.CV was significantly different among different groups（P〈0.001）.Strong correlation with age was found in ECD,Smax,Savg,Ssd and ***：Cornea of Chinese tree shrews had half CCT of human cornea and similar ECD,CV and size of corneal endothelial *** adult tree shrews had higher ECD,HG% and low ***,Smax,Savg,Ssd and CV correlated with age signifi cantly.

关键词： comea endothelial cells Chinese tree shrew( Tupaia belangeri chinensis) specular microscope age

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Regeneration of islet β-cells in tree shrews and rats

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Animal Models and Experimental Medicine 2018年第2期1卷 152-161页

作者： Yu-Qiong Zhao Ya-Qian Liu Ji-Fang Yuan Xin Dai Miao-Miao Niu Xiao-Mei Sun de-xuan kuang Wen-Guang Wang Pin-Fen Tong Na Li Lei Xiang Yun-Xiao Jia Jie-Jie Dai Hua Chen Chinese PLA General Hospital Laboratory Animal Center The Institute of Medical Biology The Chinese Academy of Medical Science and Peking Union Medical College Yunnan Key Laboratory of Vaccine Research and development on Severe Infectious Diseases Center of Tree Shrew Germplasm Resources State Key Laboratory of Kidney Diseases Chinese PLA General Hospital

Backgroud: Current understanding of injury and regeneration of islet β-cells in diabetes is mainly based on rodent studies. The tree shrew is now generally accepted as being among the closest living relatives of primates, and has been widely used in animal experimentation. However, there are few reports on islet cell composition and regeneration of β-cells in tree ***: In this study, we examined the changes in islet cell composition and regeneration of β-cells after streptozotocin(STZ) treatment in tree shrews compared with Sprague-Dawley rats. Injury and regeneration of islet β-cells were observed using hematoxylin and eosin(HE) staining and immunohistochemical staining for insulin, glucagon, somatostatin and ***: Our data showed that in rats islet injury was most obvious on day 3 after injection, and islet morphologies were significantly restored by day 21. Regeneration of islet β-cells was very pronounced in rats, and mainly involved regeneration of centro-acinar cells and transformation of extra-islet ductal cells. In tree shrews, the regeneration of islet β-cells was not as significant. On days 3 and 7, only scattered regenerated cells were observed in the remaining islets. Further, no regeneration of centro-acinar cells was ***: The results suggest that the repair mechanism of islet β-cells in tree shrews is similar to that of humans.

关键词： β-cell rat regeneration tree shrew

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A tree shrew model for steroid-associated osteonecrosis

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Zoological Research 2020年第5期41卷 564-568页

作者： Qi Chen Zhao-Xia Ma Li-Bin Xia Zhen-Ni Ye Bao-Ling Liu Tie-Kun Ma Peng-Fei Bao Xing-Fei Wu Cong-Tao Yu Dai-Ping Ma Yuan-Yuan Han Wen-Guang Wang de-xuan kuang Jie-Jie Dai Rong-Ping Zhang Min Hu Hong Shi Wen-Lin Wang Yan-Jiao Li Yunnan Key Laboratory of Primate Biomedical Research Institute of Primate Translational MedicineKunming University of Science and TechnologyKunmingYunnan 650500China Yunnan Key Laboratory for Basic Research on Bone and Joint Diseases&Yunnan Stem Cell Translational Research Center Kunming UniversityKunmingYunnan 650214China department Obstetrics Second Affiliated Hospital of Kunming Medical UniversityKunmingYunnan 650101China department of Nuclear Medicine First Affiliated Hospital of Kunming Medical UniversityKunmingYunnan 650031China Center of Tree Shrew Germplasm Resources Institute of Medical BiologyChinese Academy of Medical Sciences and Peking Union Medical CollegeKunmingYunnan 650118China School of Chinese Materia Medica Yunnan University of Chinese MedicineKunmingYunnan 650500China Kunming Medical University KunmingYunnan 650500China

deAR EDITOR,Osteonecrosis is a common human disease in *** is difficult to treat,and half of patients may need artificial joint replacement,resulting in a considerable economic burden and a reduction in quality of *** are one of the major causes of osteonecrosis and high doses of corticosteroids are considered the most dangerous factor.

关键词： doses osteonecrosis artificial

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基于数据挖掘技术的拖拉机发动机故障诊断

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农机化研究 2025年第2期47卷 244-248页

作者：匡伟祥郴州职业技术学院现代装备制造学院湖南郴州423000

拖拉机是农业生产的重要工具,发动机是其核心部件,如发动机出现故障,将会直接影响农业生产效率和产量。为此,提出了一种使用数据挖掘技术进行拖拉机发动机故障诊断的方法。利用了机器学习技术和统计学,首先针对拖拉机田间运行信号噪音... 详细信息

拖拉机是农业生产的重要工具,发动机是其核心部件,如发动机出现故障,将会直接影响农业生产效率和产量。为此,提出了一种使用数据挖掘技术进行拖拉机发动机故障诊断的方法。利用了机器学习技术和统计学,首先针对拖拉机田间运行信号噪音较大的问题,引入小波阈值去噪的方法;其次,基于卷积神经网络模型,引入一种注意力机制,提高故障诊断准确率,并通过对拖拉机传感器数据进行分析,可以帮助诊断和预测发动机故障;最后,通过实验结果验证了算法的有效性。研究结果不仅可以提高故障的准确性和效率,还能够节约维修成本和提高机器的利用率,具有较高的应用价值。

关键词：拖拉机发动机故障诊断数据挖掘技术机器学习特征选择

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Fabrication and sintering behavior of high-nitrogen nickel-free stainless steels by metal injection molding

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International Journal of Minerals,Metallurgy and Materials 2010年第4期17卷 423-428页

作者： Zi-wei Xu Cheng-chang Jia Chun-jiang kuang xuan-hui Qu School of Materials Science and Engineering University of Science and Technology Beijing Beijing 100083 China Powder Metallurgy Division Advanced Technology ＆ Materials Co. Ltd. Beijing 100081 China

High-nitrogen nickel-free stainless steels were fabricated by the metal injection molding technique using high nitrogen alloying powders and a mixture of three polymers as *** of metal powders and binders with various proportions were also investigated, and an optimum powder loading capacity was determined as 64vol%.Intact injection molded compacts were successfully obtained by regulating the processing *** debinding process for molded compacts was optimized with a combination of thermo-gravimetric analysis and differential scanning calorimetry *** optimum relative density and nitrogen content of the specimens are obtained at 1360℃,which are 97.8%and 0.79wt%,respectively.

关键词： metal injection molding debinding stainless steel sintering behavior sintered density nitrogen content

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Etomidate protects retinal ganglion cells from hydrogen peroxide-induced injury via Nrf2/HO-1 pathway

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International Journal of Ophthalmology(English edition) 2024年第9期17卷 1606-1613页

作者： xuan Zhao de-Gang Fan Xin-Chao Zhang Si-Wei You Fang kuang Ming-Mei Wu department of Histology and Embryology School of Basic Medical SciencesXi’an Medical UniversityXi’an 710021Shaanxi ProvinceChina department of Neurobiology the Basic Medical Science Academythe Fourth Military Medical UniversityXi’an 710032Shaanxi ProvinceChina department of Spinal Surgery Honghui HospitalXi’an Jiaotong UniversityXi’an 710054Shaanxi ProvinceChina College of Life Sciences Northwestern UniversityXi’an 710069Shaanxi ProvinceChina

AIM:To determine whether etomidate(ET)has a protective effect on retinal ganglion cells(RGCs)injured with hydrogen peroxide(H_(2)O_(2))and to explore the potential mechanism underlying the antioxidative stress effect of ***:Cultured RGCs were identified by double immunofluorescent labeling of microtubule-associated protein 2 and *** injury model of H_(2)O_(2)-induced RGCs oxidative stress was established in *** were pretreated with different concentrations of ET(1,5,and 10μmol/L)for 4h,followed by further exposure to H_(2)O_(2)at 1000μmol/*** counting kit 8 and Annexin V/propidium iodide assays were applied to detect the viabilities and apoptosis rates of the RGCs at 12,24,and 48h after H_(2)O_(2)*** levels of nitric oxide,malondialdehyde,and glutathione in culture media were measured at these time *** reverse transcription polymerase chain reaction(qRT-PCR)and Western blot were performed to observe the effects of ET on the messenger RNA and protein expression of inducible nitric oxide synthase(iNOS),nuclear factor erythroid 2-related factor 2(Nrf2),heme oxygenase 1(HO-1),glutathione peroxidase 1 and the level of conjugated acrolein in RGCs at 12,24,and 48h after H_(2)O_(2)stimulation and in the retina at 12h after optic nerve transection(ONT).RESULTS:The applications of 5 and 10μmol/L of ET significantly increased the viability of *** from qRT-PCR indicated a decrease in the expression of iNOS and an increase in the expressions of Nrf2 and HO-1 in ETpretreated RGCs at 12,24 and 48h after H_(2)O_(2)stimulation,as well as in ET-treated retinas at 12h after *** blot analysis revealed a decrease in the expression of iNOS and levels of conjugated acrolein,along with an increase in the expressions of Nrf2 and HO-1 in ET-pretreated RGCs in vitro and ET-treated retinas in ***:ET is a neuroprotective agent in primary cultured RGCs injured by H_(2)O_(2).The effect of ET is dosedependent with the

关键词： etomidate retinal ganglion cell neuroprotection hydrogen peroxide-induced injury nuclear factor erythroid 2-related factor 2 heme oxygenase 1

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Protective effect of ginsenoside Rg1 on 661W cells exposed to oxygen-glucose deprivation/reperfusion via keap1/nrf2 pathway

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International Journal of Ophthalmology(English edition) 2023年第7期16卷 1026-1033页

作者： Ming Zhou Xin-Qi Ma Yi-Yu Xie Jia-Bei Zhou Xie-Lan kuang Huang-xuan Shen Chong-de Long State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangdong Provincial Key Laboratory of Ophthalmology and Visual ScienceGuangzhou 510060China

AIM:To construct an in vitro model of oxygen-glucose deprivation/reperfusion(OGD/R)induced injury to the optic nerve and to study the oxidative damage mechanism of ischemia-reperfusion(I/R)injury in 661W cells and the protective effect of ginsenoside ***:The 661W cells were treated with different concentrations of Na2S2O4 to establish OGD/R model in ***,intracellular reactive oxygen species(ROS)levels and superoxide dismutase(SOD)levels were measured at different time points during the reperfusion injury *** injury model was pretreated with graded concentrations of ginsenoside ***-time polymerase chain reaction(PCR)was used to measure the expression levels of cytochrome C(cyt C)/B-cell lymphoma-2(Bcl2)/Bcl2 associated protein X(Bax),heme oxygenase-1(HO-1),caspase9,nuclear factor erythroid 2-related factor 2(nrf2),kelch-like ECH-associated protein 1(keap1)and other *** blot was used to detect the expression of nrf2,phosphorylated nrf2(pnrf2)and keap1 protein ***:Compared to the untreated group,the cell activity of 661W cells treated with Na2S2O4 for 6 and 8h decreased(Pdecreased significantly(Pde Rg1 reversed the cell viability and SOD levels in comparison to the Na_(2)S_(2)O_(4)treated group(P<0.01).Moreover,Rg1 reduced the levels of caspase3,caspase9,and cyt C,while increasing the Bcl2/Bax *** differences were all statistically significant(P<0.05).Western blot analysis showed no significant difference in the protein expression levels of keap1 and nrf2 with Rg1 treatment,however,Rg1 significantly increased the ratio of pnrf2/nrf2 protein expression compared to the Na_(2)S_(2)O_(4)treated group(P<0.001).CONCLUSION:The OGD/R process is induced in 661W cells using Na_(2)S_(2)O_(4).Rg1 inhibits OGD/R-induced oxidative damage and alleviates the extent of apoptosis in 661W cells through the keap1/nrf2 pathway

关键词： oxygen-glucose deprivation/reoxygenation ginsenoside Rg1 oxidative stress phosphorylated nrf2

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Etomidate affects the anti-oxidant pathway to protect retinal ganglion cells after optic nerve transection

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Neural Regeneration Research 2019年第11期14卷 2020-2024页

作者： xuan Zhao Fang kuang Yi-Yan You Ming-Mei Wu Si-Wei You department of Neurobiology and Collaborative Innovation Center for Brain Science Air Force Medical UniversityXi'anShaanxi ProvinceChina department of Histology and Embryology & Shaanxi Key Laboratory of Brain Disorders Xi'an Medical UniversityXi'anShaanxi ProvinceChina Ernest Mario School of Pharmacy Rutgersthe State University of New JerseyPiscatawayNJUSA

Our previous studies revealed that etomidate, a non-barbiturate intravenous anesthetic agent, has protective effects on retinal ganglion cells within 7 days after optic nerve transection. Whether this process is related to anti-oxidative stress is not clear. To reveal its mechanism, we established the optic nerve transection injury model by transecting 1 mm behind the left eyeball of adult male Sprague-Dawley rats. The rats received an intraperitoneal injection of etomidate(4 mg/kg) once per day for 7 days. The results showed that etomidate significantly enhanced the number of retinal ganglion cells retrogradely labeled with Fluorogold at 7 days after optic nerve transection. Etomidate also significantly reduced the levels of nitric oxide and malonaldehyde in the retina and increased the level of glutathione at 12 hours after optic nerve transection. Thus, etomidate can protect retinal ganglion cells after optic nerve transection in adult rats by activating an anti-oxidative stress response. The study was approved by the Animal Ethics Committee at Air Force Medical University, China(approval No. 20180305) on March 5, 2018.

关键词： nerve regeneration etomidate retinal ganglion cells optic nerve transection anti-oxidative stress nitric oxide malonaldehyde glutathione neural regeneration

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Sandwich-structured nanocomposites of N-doped graphene and nearly monodisperse Fe304 nanoparticles as high-performance Li-ion battery anodes

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Nano Research 2017年第9期10卷 2923-2933页

作者： Wen Qi xuan Li Hui Li Weikang Wu Pei Li Ying Wu Chunjiang kuang Shaoxiong Zhou Xiaolin Li Beijing Key Laboratory of Energy Nanomaterials Advance Technology & Materials Co. Ltd. China Iron & Steel Research Institute Group Beijing 10008 I China School of Materials Science and Engineering Tianjin University Tianjin 300072 China Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials Ministry of Education Shandong University Jinan250061 China department of Stationary Energy Storage Pacific Northwest National Laboratory Richland Washington 99354 USA

Iron oxides have attracted considerable interest as abundant materials for high-capacity Li-ion battery anodes. However, their fast capacity fading owing to poorly controlled reversibility of the conversion reactions greatly hinders their application. Here, a sandwich-structured nanocomposite of N-doped graphene and nearly monodisperse Fe3O4 nanoparticles were developed as high-performance Li-ion battery anode. N-doped graphene serves as a conducting framework for the self-assembled structure and controls Fe3O4 nucleation through the interaction of N dopants, surfactant molecules, and iron precursors. Fe3O4 nanoparticles were well dispersed with a uniform diameter of ~15 nm. The unique sandwich structure enables good electron conductivity and Li-ion accessibility and accommodates a large volume change. Hence, it delivers good cycling reversibility and rate performance with a capacity of ~1,227 mA·h·g–1 and 96.8% capacity retention over 1,000 cycles at a current density of 3 A·g–1. Our work provides an ideal structure design for conversion anodes or other electrode materials requiring a large volume change.

关键词： N-doped graphene iron oxides self-assembly Li-ion battery density functional theory

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Mapping global impervious surface area and green space within urban environments

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Science China Earth Sciences 2019年第10期62卷 1591-1606页

作者： Wenhui kuang Key Laboratory of Land Surface Pattern and Simulation Institute of Geographic Sciences and Natural Resources Research Chinese Academy of Sciences

The mapping of impervious surface area(ISA) and urban green space(UGS) is essential for improving the urban environmental quality toward ecological, livable, and sustainable goals. Currently, accurate ISA and UGS products are lacking in urban areas at the global scale. This study established regression models that estimated the fraction of ISA/UGS in global 30 cities for validation using MODIS NDVI and DMSP/OLS nighttime light imageries. A global dataset of ISA and UGS fraction with a spatial resolution of 250 m×250 m was developed using the regression model, with a mean relative error of 0.19 for its ISA. The results showed the global urban area of 76.29×10~4 km^2, which was primarily distributed in central Europe, eastern Asia,and central and eastern North America. The urban land area in North America, Europe, and Asia was 66.3×10~4 km^2, accounting for 86.91% of the world’s urban area;the urban land area of the top 50 countries accounted for 59.32% of the total urban land area in the world. The global ISA of 45.26×10~4 km^2 was mainly distributed in central and southern North America, eastern Asia, and Europe, as well as coastal regions around the world. The proportion of ISA situated in built-up areas on the continental scale followed the order of Africa(>70%)>South America>Oceania>Asia(>60%)>North America>Europe(>50%), and these areas were mostly in southeastern North America, southwestern Europe, and eastern and western Asia. North America, Europe, and Asia accounted for 89.44% of the world’s total UGS. The cities of developed countries in Europe and North America exposed a dramatic mosaic of ISA and UGS composites in urban construction. Therefore, the proportion of UGS is relatively high in those cities. However, in developing and underdeveloped countries, the proportion of UGS in built-up areas is relatively low, and urban environments need to be improved for livability.

关键词： Impervious surface area Green space Habitat environment Remote sensing classification Global scale

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