Regeneration of islet β-cells in tree shrews and rats

作     者：Yu-Qiong Zhao Ya-Qian Liu Ji-Fang Yuan Xin Dai Miao-Miao Niu Xiao-Mei Sun De-Xuan Kuang Wen-Guang Wang Pin-Fen Tong Na Li Lei Xiang Yun-Xiao Jia Jie-Jie Dai Hua Chen 

作者机构：Chinese PLA General HospitalLaboratory Animal Center The Institute of Medical Biology The Chinese Academy of Medical Science and Peking Union Medical College Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases Center of Tree Shrew Germplasm Resources State Key Laboratory of Kidney DiseasesChinese PLA General Hospital 

出 版 物：《Animal Models and Experimental Medicine》 (动物模型与实验医学（英文）)

年 卷 期：2018年第1卷第2期

页      面：152-161页

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Laboratory Animal Science of PLA,Grant/Award Number:SYDW-0009 The National Science and Technology Support Program,Grant/Award Number:2011BAI15B01,2014BAI01B01 

主　　题：β-cell rat regeneration tree shrew 

摘      要：Backgroud: Current understanding of injury and regeneration of islet β-cells in diabetes is mainly based on rodent studies. The tree shrew is now generally accepted as being among the closest living relatives of primates, and has been widely used in animal experimentation. However, there are few reports on islet cell composition and regeneration of β-cells in tree ***: In this study, we examined the changes in islet cell composition and regeneration of β-cells after streptozotocin(STZ) treatment in tree shrews compared with Sprague-Dawley rats. Injury and regeneration of islet β-cells were observed using hematoxylin and eosin(HE) staining and immunohistochemical staining for insulin, glucagon, somatostatin and ***: Our data showed that in rats islet injury was most obvious on day 3 after injection, and islet morphologies were significantly restored by day 21. Regeneration of islet β-cells was very pronounced in rats, and mainly involved regeneration of centro-acinar cells and transformation of extra-islet ductal cells. In tree shrews, the regeneration of islet β-cells was not as significant. On days 3 and 7, only scattered regenerated cells were observed in the remaining islets. Further, no regeneration of centro-acinar cells was ***: The results suggest that the repair mechanism of islet β-cells in tree shrews is similar to that of humans.