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Ultra-high spatio-temporal resolution imaging with parallel acquisition-readout structured illumination microscopy(PAR-SIM)

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Light(Science & Applications) 2024年第6期13卷 1140-1158页

作者： Xinzhu Xu Wenyi Wang Liang Qiao Yunzhe Fu Xichuan Ge Kun Zhao Karl Zhanghao Meiling Guan Xin Chen Meiqi Li dayong jin Peng Xi Department of Biomedical Engineering College of Future TechnologyPeking UniversityBeijing100871China Wallace H.Coulter Dept.of Biomedical Engineering Georgia Institute of Technology and Emory UniversityAtlanta30332GAUSA National Biomedical Imaging Center Peking UniversityBeijing100871China Department of Biomedical Engineering College of EngineeringSouthern University of Science and TechnologyShenzhen518055GuangdongChina Airy Technologies Co. Ltd.Beijing100086China Eastern Institute for Advanced Study Eastern Institute of TechnologyNingboZhejiang315200China School of Life Science Peking UniversityBeijing100871China Institute for Biomedical Materials and Devices(IBMD) Faculty of ScienceUniversity of Technology SydneySydneyNSW2007Australia

Structured illumination microscopy(SIM)has emerged as a promising super-resolution fluorescence imaging technique,offering diverse configurations and computational strategies to mitigate phototoxicity during real-time imaging of biological *** efforts to enhance system frame rates have concentrated on processing algorithms,like rolling reconstruction or reduced frame reconstruction,or on investments in costly sCMOS cameras with accelerated row readout *** this article,we introduce an approach to elevate SIM frame rates and region of interest(ROI)coverage at the hardware level,without necessitating an upsurge in camera expenses or intricate ***,parallel acquisition-readout SIM(PAR-SIM)achieves the highest imaging speed for fluorescence imaging at currently available detector *** using the full frame-width of the detector through synchronizing the pattern generation and image exposure-readout process,we have achieved a fundamentally stupendous information spatial-temporal flux of 132.9 MPixels·s^(−1),9.6-fold that of the latest techniques,with the lowest SNR of−2.11 dB and 100 nm ***-SIM demonstrates its proficiency in successfully reconstructing diverse cellular organelles in dual excitations,even under conditions of low signal due to ultra-short exposure ***,mitochondrial dynamic tubulation and ongoing membrane fusion processes have been captured in live COS-7 cell,recorded with PAR-SIM at an impressive 408 *** posit that this novel parallel exposure-readout mode not only augments SIM pattern modulation for superior frame rates but also holds the potential to benefit other complex imaging systems with a strategic controlling approach.

关键词： hardware illumination resolution

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“Perspective”–A new approach to serve our Light community

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Light(Science & Applications) 2018年第1期7卷 220-221页

作者： dayong jin Jianlin Cao Faculty of Science Institute for Biomedical Materials and Devices(IBMD)University of Technology SydneySydneyNew South Wales 2007Australia State Key Laboratory of Applied Optics CIOMPCAS.No.3888Dongnanhu RoadChangchunChina

Scientists are known as the idea explorers,technology inventors and visionaries for the great benefits of our end-users and *** are optimistic,realistic and scientifically *** way for them to create new ideas and technologies can be through their intuitions and others’guidance and *** scientific publishing plays an essential role in effective communications and knowledge *** the past decade,rapid growth occurred in our science communities and the number of research *** pursuing quantity of publishing may be in lack of satisfaction,given the large volume of information produced becomes overwhelming to our early career researchers,students or cross-disciplinary ***-tier journals should stay more closely connected with our most creative authors and influential research leaders,and find ways to boost more interdisciplinary discussions and inspire creative thoughts and practice.

关键词： discussions rigorous publishing

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Super-resolution fluorescence polarization microscopy

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Journal of Innovative Optical Health Sciences 2018年第1期11卷 1-12页

作者： Karl Zhanghao Juntao Gao dayong jin Xuedian Zhang Peng Xi Department of Biomedical Engineering College of Engineering Peking UniversityP.R.China School of Optical-Electronic and Computer Engineering Shanghai University of Science and Technology P.R.China Department of Automation Tsinghua University Beijing 100084P.R.China Bioinfomatics Division TNLIST MOE Key Laboratory of Bioinformatics and Center for Synthetic&System Biology Tsinghua UniversityBeijing 100084P.R.China Faculty of Science Institute for Biomedical Materials and Devices(IBMD)University of TechnologyAustralia

Fluorescence polarization is related to the dipole orientation of chromophores,making fuores-cence polarization microscopy possible to_reveal structures and functions of tagged cellularorganelles and biological *** recent super resolution techniques have beenapplied to fluorescence polarization microscopy,achieving dipole measurement at *** this review,we summarize both difraction limited and super resolution fluorescence polari-zation microscopy techniques,as well as their applications in biological imaging.

关键词： Fluorescence polarization microscopy super resolution fluorescence anisotropy linear dichroism polarization modulation

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Polarized upconversion emission at metasurface

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Light(Science & Applications) 2023年第11期12卷 2277-2279页

作者： Zhichao Yang dayong jin Institute for Biomedical Materials&Devices Faculty of ScienceUniversity of Technology SydneyUltimoNSW 2007Australia

Leveraging the resonant modes of all-dielectric metasurfaces,specifically quasi-bound state in the continuum and Mie resonances,the precise orthogonal polarization control has been realized.

关键词： resonant precise dielectric

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船舶甲板机械电动机的应用与适应性设计制造

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防爆电机 2024年第2期59卷 24-26页

作者：刘宝芳何荣全陈金刚靳大勇德州恒力电机有限责任公司山东德州253005

主要讲述船舶甲板机械电动机的应用和设计特点,在电磁设计,材料选择,适应性设计,制造工艺的控制和电机的防水设计方面分别进行论述。说明船舶甲板机械电动机需要注意的特殊设计要点。

关键词：船用甲板机械电磁设计工艺措施防水结构绝缘结构

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A homogeneous DNA assay by recovering inhibited emission of rare earth ions-doped upconversion nanoparticles

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Journal of Rare Earths 2019年第1期37卷 11-18,M0002页

作者： Yingzhu Zhou Yinghui Chen Hao He iayan Liao Hien T.T.Duong Maryam Parviz dayong jin Institute for Biomedical Materials and Devices School of Mathematical and Physical Sciences Faculty of Science University of Technology Sydney ARC Research Hub for Integrated Device for End-User Analysis at Low-levels Faculty of Science University of Technology Sydney The Faculty of Pharmacy The University of Sydney

Robust and easy-to-use kits specific for a particular DNA sequence are desirable for early detection of diseases. However, the major challenge with these tests is often the background fluorescence artifacts arising from biological species due to employing UV and visible range of light. Here, we have reported a near-infrared (NIR) fluorescence "turn-on" kit based on rare earth ions doped nanoparticles, upconversion nanoparticles (UCNPs), and gold nanoparticles (AuNPs), which forms a fluorescence-quencher pair,brought together by a hairpin structure through the formation of double-stranded DNA (dsDNA), with quenched upconversion luminescence. In the presence of analytes, the molecular beacon opens to push AuNPs away from UCNPs, with a distance longer than the efficient quenching distance, so that the inhibited upconversion emission will be restored. We demonstrated that this assay provides a homogeneous, facile, simple and highly selective HIV-1 based DNA detection system with restore efficiency up to 85%, and the detection limit of 5 nm.

关键词： Upconversion nanoparticles Gold nanoparticles LRET Homogenous assay DNA assay Fluorescence recovery

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Viral inactivation by light

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eLight 2022年第1期2卷 237-254页

作者： Mohammad Sadraeian Le Zhang Farzaneh Aavani Esmaeil Biazar dayong jin Present Address:Institute for Biomedical Materials and Devices(IBMD) Faculty of ScienceUniversity of Technology SydneySydneyNSW 2007Australia Department of Oral and Maxillofacial Surgery Division of Regenerative Orofacial MedicineUniversity Hospital Hamburg-Eppendorf20251 HamburgGermany Department of Biomedical Engineering Islamic Azad UniversityTonekabon BranchTonekabonIran UTS‑SUStech Joint Research Centre for Biomedical Materials&Devices Department of Biomedical EngineeringCollege of EngineeringSouthern University of Science and TechnologyShenzhenGuangdongChina

Nowadays,viral infections are one of the greatest challenges for medical sciences and human *** antiviral compounds and chemical inactivation remain inadequate,physical approaches based on irradiation provide new potentials for prevention and treatment of viral infections,without the risk of drug resistance and other unwanted side *** across the electromagnetic spectrum can inactivate the virions using ionizing and non-ionizing *** review highlights the anti-viral utility of radiant methods from the aspects of ionizing radiation,including high energy ultraviolet,gamma ray,X-ray,and neutron,and non-ionizing photo-inactivation,including lasers and blue light.

关键词： Antiviral therapy Enveloped virus Photo-inactivation Ionizing radiation Nuclear radiations

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Two-photon MINFLUX with doubled localization precision

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eLight 2022年第1期2卷 58-67页

作者： Kun Zhao Xinzhu Xu Wei Ren dayong jin Peng Xi Department of Biomedical Engineering College of Future TechnologyPeking UniversityBeijing 100871China Wallace H.Coulter Department of Biomedical Engineering Georgia Institute of Technology and Emory UniversityAtlanta30332 GeorgiaUSA UTS‑SUStech Joint Research Centre for Biomedical Materials&Devices Department of Biomedical EngineeringCollege of EngineeringSouthern University of Science and TechnologyShenzhenGuangdong 518055China National Biomedical Imaging Center Peking UniversityBeijing 100871China

Achieving localization with molecular precision has been of great interest for extending fluorescence microscopy to *** pioneers this transition through point spread function(PSF)engineering,yet its performance is primarily limited by the signal-to-background *** we demonstrate theoretically that two-photon MINFLUX(2p-MINFLUX)could double its localization precision through PSF engineering by nonlinear ***ér-Rao Bound(CRB)is studied as the maximum localization precision,and CRB of two-photon MINFLUX is halved compared to single-photon MINFLUX(1p-MINFLUX)in all three ***,in order to achieve same localization precision with 1p-MINFLUX,2p-MINFLUX requires only 1/4 of fluorescence *** simultaneous two-photon excitation of multiple fluorophore species,2p-MINFLUX may have the potential for registration-free nanoscopy and multicolor tracking.

关键词： Super-resolution microscopy Single-molecule localization MINFLUX Two-photon microscopy

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Contrast-enhanced fluorescence microscope by LED integrated excitation cubes

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Light(Advanced Manufacturing) 2023年第2期4卷 18-27页

作者： Yuanhua Liu Xiang Zhang Fei Su Zhiyong Guo dayong jin UTS-SUSTech Joint Research Centre for Biomedical Materials and Devices Department of Biomedical EngineeringSouthern University of Science and TechnologyShenzhenGuangdong 518055China Institute for Biomedical Materials and Devices(IBMD) Faculty of ScienceUniversity of Technology SydneyNSW 2007Australia

Fluorescence microscopy is a powerful tool for scientists to observe the microscopic world,and the fluorescence excitation light source is one of the most critical *** compensate for the short operation lifetime,integrated light sources,and low excitation efficiency of conventional light sources such as mercury,halogen,and xenon lamps,we designed an LED-integrated excitation cube(LEC)with a decentralized structure and high optical power *** a Fresnel lens,the light from the light-emitting diode(LED)was effectively focused within a 15 mm mounting distance to achieve high-efficiency *** can be easily designed in the shape of fluorescence filter cubes for installation in commercial fluorescence ***’optical efficiency is 1–2 orders of magnitude higher than that of mercury lamps;therefore,high-quality fluorescence imaging with spectral coverage from UV to red can be *** replacing conventional fluorescence filter cubes,LEC can be easily installed on any commercial fluorescence microscope.A built-in LEC driver can identify the types of LEDs in different spectral bands to adopt the optimal operating current and frequency of ***,high-contrast images can be achieved in pulse mode by time-gated imaging of long-lifetime luminescence.

关键词： LED-integrated excitation cube Filter cube Time-gated imaging Fluorescence microscopy

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Silica shell-assisted synthetic route for mono-disperse persistent nanophosphors with enhanced in vivo recharged near-infrared persistent luminescence

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Nano Research 2017年第6期10卷 2070-2082页

作者： Rui Zou Junjian Huang Junpeng Shi Lin Huang Xuejie Zhang Ka-Leung Wong Hongwu Zhang dayong jin jing Wang Qiang Su Ministry of Education Key Laboratory of Bioinorganic and Synthetic Chemistry State Key Laboratory of Optoelectronic Materials and Technologies KL GHEI of Environment and Energy Chemistry School of Chemistry Sun Yat-Sen University Guangzhou 510275 China Key Lab of Urban Pollutant Conversion Institute of Urban Environment Chinese Academy of Sciences Xiamen 361021 China Department of Chemistry Hong Kong Baptist University Hong Kong 999077 China Institute for Biomedical Materials and Devices (IBMD) Faculty of Science University of Technology Sydney NSW 2007 Australia

Near-infrared （NIR） persistent-luminescence nanoparticles have emerged as a new class of background-free contrast agents that are promising for in vivo imaging. The next key roadblock is to establish a robust and controllable method for synthesizing monodisperse nanoparticles with high luminescence brightness and long persistent duration. Herein, we report a synthesis strategy involving the coating/etching of the SiO2 shell to obtain a new class of small NIR highly persistent luminescent ZnGa2O4：Cr^3＋,Sn^4＋（ZGOCS） nanoparticles. The optimized ZGOCS nanoparticles have an excellent size distribution of -15 nm without any agglomeration and an NIR persistent luminescence that is enhanced by a factor of 13.5, owing to the key role of the SiO2 shell in preventing nanoparticle agglomeration after annealing. The ZGOCS nanoparticles have a signal-to-noise ratio -3 times higher than that of previously reported ZnGa204：Cr^3＋（ZGC-1） nanoparticles as an NIR persistent-luminescence probe for in vivo bioimaging. Moreover, the persistent-luminescence signal from the ZGOCS nanoparticles can be repeatedly re-charged in situ with external excitation by a white light- emitting diode; thus, the nanopartides are suitable for long-term in vivo imaging applications. Our study suggests an improved strategy for fabricating novel high-performance optical nanoparticles with good biocompatibility.

关键词： core-shell structure in vivo imaging narrow size distribution near-infrared (NIR)persistent luminescence biocompatibility

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