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Advances on systems metabolic engineering of Bacillus subtilis as a chassis cell

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Synthetic and Systems Biotechnology 2020年第4期5卷 245-251页

作者： Mengjie Xiang Qian Kang dawei Zhang Tianjin Institute of Industrial Biotechnology Chinese Academy of SciencesTianjin300308People's Republic of China Key Laboratory of Systems Microbial Biotechnology Chinese Academy of SciencesTianjin300308People's Republic of China University of Chinese Academy of Sciences Beijing100049People's Republic of China

The Gram-positive model bacterium Bacillus subtilis,has been broadly applied in various fields because of its low pathogenicity and strong protein secretion ability,as well as its well-developed fermentation *** is considered as an attractive host in the field of metabolic engineering,in particular for protein expression and secretion,so it has been well studied and applied in genetic *** this review,we discussed why *** is a good chassis cell for metabolic *** also summarized the latest research progress in systematic biology,synthetic biology and evolution-based engineering of ***,and showed systemic metabolic engineering expedite the harnessing *** for bioproduction.

关键词： Bacillus subtilis Systems metabolic engineering Systematic biology Synthetic biology Evolutionary engineering

Natural attenuation characteristics and comprehensive toxicity changes of C9 aromatics under simulated marine conditions

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Journal of Environmental Sciences 2021年第11期33卷 26-35页

作者： dawei Li Jiangyue Wu Jiaqi Liu Aifeng Li Fanping Meng Key Laboratory of Marine Environment and Ecology Ministry of EducationOcean University of ChinaQingdao 266100China College of Environmental Science and Engineering Ocean University of ChinaQingdao 266100China National Marine Hazard Mitigation Service Ministry of Natural Resource of the People’s Republic of ChinaBeijing 100194China

Microcosmic experiments were performed under a simulated marine environment to investigate the natural attenuation of C9 aromatics using nine components(propylbenzene,isopropylbenzene,2-ethyltoluene,3-ethyltoluene,4-ethyltoluene,1,2,3-trimethylbenzene,1,2,4-trimethylbenzene,1,3,5-trimethylbenzene,and indene).This research aims to assess the contribution of biodegradation and abiotic activity to total attenuation of C9 aromatics and ascertain the changes in the comprehensive toxicity of seawater in the natural *** process of natural attenuation indicates the agreement with pseudo-first-order kinetics for all nine components in microcosmic *** half-lives of the nine main compounds in C9 aromatics ranged between 0.34 day and 0.44 day under optimal *** experiments showed that the natural attenuation of nine aromatic hydrocarbonsmainly occurred via abiotic *** samples significantly inhibited the luminescence of ***(the luminescence inhibition ratio reached 100%)at the beginning of the *** addition,the toxicity declined slowly and continued for 25 *** attenuation kinetics and changes in toxicity could be applied to explore the natural attenuation of C9 aromatics in the marine environment.

关键词： C9 aromatics Reaction kinetics Marine Microcosm Toxicity changes

Hyperextended crustal structure of the Qiongdongnan Basin and subsequent magmatic influence from the Hainan mantle plume

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Science China Earth Sciences 2022年第5期65卷 845-862页

作者： Shaohong XIA Chaoyan FAN dawei WANG Jinghe CAO Fang ZHAO Key Laboratory of Ocean and Marginal Sea Geology South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301China Sanya Institute of Oceanology SCSIOSanya 572100China Institute of Deep-sea Science and Engineering Chinese Academy of SciencesSanya 572000China

The Qiongdongnan Basin(QDNB)is a rift basin located in the extension direction of the oceanic ridge of the Northwest Subbasin of the South China *** basin is surrounded by Late Cenozoic Ocean Island Basalts(OIBs)magmatism associated with the putative Hainan mantle ***,how the Hainan mantle plume has affected the QDNB has not been studied in *** reveal the crustal structure of the QDNB and the possible influence of the Hainan mantle plume,we conducted a wide-angle seismic exploration from Hainan Island across the QDNB to the Xisha Block,and obtained the crustal structure by travel-time *** results show that the crustal stretching factors of the Northern and Southern Rises of the QDNB are estimated to be 1.3-2.0,indicating slight or moderate crustal ***,the crustal stretching factor in the Central Depression of the QDNB is estimated to be more than 3.0,which means that the crust has been *** by two detachment faults(F2 and F11),the upper and lower crust show brittle and ductile thinning,*** anomalous low-velocity conduits penetrating the crystalline crust are developed in the Songnan Uplift and the Southern Rise of the *** P-wave velocities are 5.5-6.0 km s^(−1),which are significantly lower than those of the surrounding crust(6.0-6.8 km s^(−1)).The Late Cenozoic magmatic intrusions(e.g.,sills and dikes)and hydrothermal activities can be observed in the sedimentary strata above these low-velocity *** interpret that the low-velocity conduits might be the crustal magmatic footprints of the Hainan mantle plume,due to the correlated distributions of OIB-type magmatism,the magmatic intrusion patterns,the deep structure of the Hainan mantle plume and the high heat flow of 95 mW m^(−2).Thus,we propose that the crustal structure of the QDNB is the product of crustal hyperextension in the rifting stage and subsequently affected by the Hainan mantle plume *** study provide

关键词： Crustal structure Hyperextension Magmatism Hainan mantle plume Qiongdongnan Basin

Telomerase as a “stemness” enzyme

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Science China(Life Sciences) 2014年第6期57卷 564-570页

作者： KONG Feng ZHENG ChengYun XU dawei Central Research Laboratory the Second Hospital of Shandong University Department of Hematology the Second Hospital of Shandong University Department of Medicine Division of Hematology and Centre for Molecular MedicineKarolinska Institutet and Karolinska UniversityHospital Solna

Pluripotent or multipotent stem cells are involved in development and tissue homeostasis;they have the ability to self-renew and differentiate into various types of functional *** maintain these properties,stem cells must undergo sustained or unlimited proliferation that requires the stabilization of telomeres,which are essential for chromosome end ***,an RNA-dependent DNA polymerase,synthesizes telomeric *** the lengthening of telomeres the lifespans of cells are extended,or indefinite proliferation is conferred;this is intimately associated with stem cell *** review highlights our current understanding of telomerase as a"stemness"enzyme and discusses the underlying implications.

关键词： stem cells telomerase telomere genomic stability iPS cells TERC TERT

Expanding application of CRISPR-Cas9 system in microorganisms

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Synthetic and Systems Biotechnology 2020年第4期5卷 269-276页

作者： Jing Zhao Huan Fang dawei Zhang Tianjin Institute of Industrial Biotechnology Chinese Academy of SciencesTianjin300308People's Republic of China Key Laboratory of Systems Microbial Biotechnology Chinese Academy of SciencesTianjin300308People's Republic of China University of Chinese Academy of Sciences Beijing100049People's Republic of China

The development of CRISPR-Cas9 based genetic manipulation tools represents a huge breakthrough in life sciences and has been stimulating research on metabolic engineering,synthetic biology,and systems *** CRISPR-Cas9 and its derivative tools are one of the best choices for precise genome editing,multiplexed genome editing,and reversible gene expression control in ***,challenges remain for applying CRISPR-Cas9 in novel microorganisms,especially those industrial microorganism hosts that are intractable using traditional genetic manipulation *** to further extend CRISPR-Cas9 to these microorganisms is being an urgent *** this review,we first introduce the mechanism and application of CRISPR-Cas9,then discuss how to optimize CRISPR-Cas9 as genome editing tools,including but not limited to how to reduce off-target effects and Cas9 related toxicity,and how to increase on-target efficiency by optimizing crRNA and sgRNA design.

关键词： CRISPR-Cas9 system Microorganisms Off-target Cas9 toxicity sgRNA

A THERMO-PLASTIC/VISCOPLASTIC DAMAGE MODEL FOR GEOMATERIALS

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Acta Mechanica Solida Sinica 2011年第3期24卷 195-208页

作者： Hui Zhou dawei Hu Fan Zhang Jianfu Shao State Key Laboratory of Geomechanics and Geotechnical Engineering Institute of Rock and Soil Mechanics Chinese Academy of Sciences Wuhan 430071 China LML UMR 8107 CNRS-University of Lille I Villeneuve d＇Aseq France School of Civil Engineering and Architecture Hubei University of Technology Wuhan 430068 China

A thermo-plastic/viscoplastic damage coupled model was formulated to describe the time independent and time dependent behaviors of geomaterials under temperature effect. The plastic strain was divided into instantaneous plastic strain and creep plastic strain. To take temperature effect into acconnt, a temperature variable was introduced into the instantaneous and creep plastic behavior descriptions and damage characterization, and a linear thermal expansion law was used in constitutive equation formulation. According to the mechanical behavior of rock salt, a specific model was proposed based on the previous model and applied to Avery rock salt, in which the numerical results obtained from our model had a good agreement with the data from experiments.

关键词： thermo-plastic/viscoplastic damage coupled mechanical model geomaterial rocksalt

An efficient synthesis of isocoumarins via a CuI catalyzed cascade reaction process

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Science China Chemistry 2009年第10期52卷 1616-1621页

作者： WANG LeiLei ZHANG XiaoJing JIANG YongWen MA dawei Shenyang Pharmaceutical University Shenyang 110016China State Key Laboratory of Bioorganic Chemistry and Natural Products Shanghai Institute of Organic ChemistryChinese Academy of SciencesShanghai 200032China

3-Alkyl isocoumarins are provided by CuI/amino acid-catalyzed Sonogashira coupling reaction of o-bromo benzoic acids and terminal alkynes and the subsequent additive cyclization. This cascade process allows synthesis ... 详细信息

关键词： coupling cascade reaction copper iodide isocoumarins

The prediction of spontaneous oil-water imbibition in composite capillary

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Petroleum 2022年第1期8卷 84-91页

作者： Lin Wang Feiying Ma Yongming He dawei Liu Petroleum Engineering College Guangdong University of Petrochemical TechnologyMaoming525000China State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation(Chengdu University of Technology) Chengdu610059China

In view of the classical Lucas-Washburn equation,which can only describe the spontaneous imbibition of single wetted capillary,a tilted composite capillary model with circular cross section,composed of different wettability capillary wall was *** model can describe the spontaneous oil-water imbibition of water-wet capillary,oil-wet capillary and mixed wetting *** numerical solution of the model equation,it is found that the component content of the capillary walls,the capillary radius and the oil-water viscosity ratio have great effects on the spontaneous oil-water *** of capillary inclination angle and inertia force on spontaneous oil-water imbibition are related to the capillary *** of capillary inclination angle and inertia force can be ignored in small radius capillary,while effects of inclination angle and inertia force can not be ignored in large radius capillary.

关键词： Non-homogeneous wettability Composite capillary walls Spontaneous imbibition Oil and water Lucas-Washburn equation

H-infinity filtering for discrete-time switched linear systems under arbitrary switching

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控制理论与应用（英文版） 2011年第2期9卷 261-266页

作者： Ding, dawei Yang, Guanghong Li, Xiaoli University of Science and Technology Beijing Beijing 100083 China College of Information Science and Engineering And State Key Laboratory of Integrated Automation for Process Industry Northeastern University Shenyang 110004 China

This paper is concerned with the problem of H-infinity filtering for discrete-time switched linear systems under arbitrary switching *** sufficient conditions for the solvability of the problem are given via switched quadratic Lyapunov *** on Finsler's lemma,two sets of slack variables with special structure are introduced to provide extra degrees of freedom in optimizing the guaranteed H-infinity *** to the existing methods,the proposed one has better performances and less *** example is given to illustrate its effectiveness.

关键词： Switched systems Switched quadratic Lyapunov function Finsler's lemma Filtering Linear matrix inequality (LMI)