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Supplementation of Lycium barbarum residue increases the growth rate of Tan sheep by enhancing their feed intake and regulating their rumen microbiome and metabolome

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Journal of Integrative Agriculture 2024年第9期23卷 3129-3144页

作者： Yajun Zhang Xiao Chang Bing Wang dawei wei Rongzhen Zhong Yansheng Guo Min Du Guijie Zhang College of Forestry and Prataculture Ningxia UniversityYinchuan 750021China Jilin Provincial Key Laboratory of Grassland Farming Northeast Institute of Geography and AgoecologyChinese Academy of SciencesChangchun 130102China State Key Laboratory of Animal Nutrition/College of Animal Science and Technology China Agricultural UniversityBeijing 100193China College of Animal Science and Technology Ningxia UniversityYinchuan 750021China Nutrigenomics and Growth Biology Laboratory Department of Animal SciencesWashington State UniversityPullmanWA 99164USA

Lycium barbarum residue(LBR),a by-product of *** processing,is packed with bioactive components and can be potentially utilized as a feed additive in animal ***,the fundamental understanding of its effectiveness on livestock animals is still lacking,particularly in *** explore the effects of LBR on the growth performance,rumen fermentation parameters,ruminal microbes and metabolites of Tan sheep,sixteen fattening rams(aged 4 mon)were fed a basal diet(CON,n=8)or a basal diet supplemented with 5%LBR(LBR,n=8).The experiment lasted for 70 d,with 10 d adaptation period and 60 d treatment *** results showed that the LBR enhanced the average daily feed intake,average daily gain(P<0.05),and ruminal total volatile fatty acids(P<0.01)while decreasing ammonia-nitrogen concentration and rumen pH value(P<0.05).Additionally,the LBR improved the relative abundances of Prevotella,Succiniclasticum,Ruminococcus,Coprococcus,Selenomonas,and Butyrivibrio(P<0.05)and reduced the relative abundances of Oscillospira and Succinivibrio(P<0.05).The LBR altered the ruminal metabolome(P<0.01)by increasing the abundances of ruminal metabolites involved in amino acids(e.g.,L-proline,L-phenylalanine,L-lysine,and L-tyrosine),pyrimidine metabolism(e.g.,uridine,uracil,and thymidine),and microbial protein synthesis(e.g.,xanthine and hypoxanthine).In conclusion,LBR had positive effects on the growth rate of Tan sheep as well as on rumen fermentation parameters,rumen microbiome and rumen metabolome.

关键词： Lycium barbarum residue rumen microbiome metabolomics growth performance Tan sheep

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Understanding the rapid spread of antimicrobial resistance genes mediated by IS26

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mLife 2024年第1期3卷 101-109页

作者： Na Tang dawei wei Yuan Zeng Gang Zhang Chao Wang Jie Feng Yuqin Song State Key Laboratory of Microbial Resources Institute of MicrobiologyChinese Academy of SciencesBejingChina College of Life Science University of Chinese Academy of SciencesBeijingChina

Insertion sequences(ISs)promote the transmission of antimicrobial resistance genes(ARGs)across bacterial ***,their contributions and dynamics during the transmission of resistance remain *** this study,we selected is26 as a representative transposable element to decipher the relationship between ISs and ARGs and to investigate their transfer features and transmission *** retrieved 2656 translocatable IS26-bounded units with ARGs(tiS26-bUs-ARGs)in complete bacterial genomes from the NCBI RefSeq *** total,124 ARGs spanning 12 classes of antibiotics were detected,and the average contribution rate of IS26 to these genes was 41.2%.We found that IS26-bounded units(IS26-bUs)mediated extensive ARG dissemination within the bacteria of the Gammaproteobacteria class,showing strong transfer po-tential between strains,species,and even *** Is26-bUs expanded in bacterial populations over time,and their temporal expansion trend was significantly correlated with antibiotic *** wide dissemination could be due to the nonspecific target site preference of ***,we experimentally confirmed that the introduction of a single copy of is26 could lead to the formation of a composite transposon mediating the transmission of“passenger”*** observations extend our knowledge of the IS26 and provide new insights into the mediating role of ISs in the dissemination of antibiotic resistance.

关键词： antibiotic consumption antibiotic resistance genes horizontal gene transfer insertion sequence IS26

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FoxO1对牛骨骼肌细胞增殖、凋亡和分化的调控

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中国农业科学 2024年第6期57卷 1191-1203页

作者：姜超张久盘宋雅萍宋小雨吴昊魏大为宁夏大学动物科技学院/宁夏反刍动物分子细胞育种重点实验室宁夏农林科学院动物科学研究所

【目的】骨骼肌是动物机体的重要组成成分，其生长发育直接影响畜禽肉产量，叉头转录因子O1(forkhead box protein O1, FoxO1)作为重要的转录调控因子，其与骨骼肌生长发育密切相关。探究过表达FoxO1对牛骨骼肌细胞增殖、凋亡与分化的... 详细信息

【目的】骨骼肌是动物机体的重要组成成分，其生长发育直接影响畜禽肉产量，叉头转录因子O1(forkhead box protein O1, FoxO1)作为重要的转录调控因子，其与骨骼肌生长发育密切相关。探究过表达FoxO1对牛骨骼肌细胞增殖、凋亡与分化的作用，为肉牛遗传改良提供基础材料。【方法】采集牛的多个组织样品，提取其RNA并反转录，利用实时荧光定量PCR(qPCR)构建FoxO1组织表达谱。利用酶消化法分离得到牛骨骼肌细胞，通过观察其分化后肌管的形成以及qPCR检测其分化标志基因的表达情况来检验所分离细胞的分化性能。利用免疫荧光技术对牛骨骼肌细胞进行FoxO1亚细胞定位。设计并包装牛FoxO1过表达腺病毒，以提高牛骨骼肌细胞内FoxO1的表达。利用EdU染色检测过表达FoxO1对细胞相对增殖率的影响。利用流式细胞术检测过表达FoxO1对细胞周期分布的影响。利用qPCR检测过表达FoxO1对牛骨骼肌细胞增殖、凋亡和分化相关基因表达水平的影响。【结果】组织表达谱结果显示FoxO1在多个组织中均有表达，其在成年牛的背脂中表达量最高，在背最长肌组织中表达量最低，且FoxO1在犊牛背最长肌组织中的表达量要极显著高于成年牛的（P<0.01）。亚细胞定位结果显示FoxO1在牛骨骼肌细胞的细胞核和细胞质内均有表达，其细胞核内荧光强度高于细胞质。成功构建FoxO1过表达载体，并完成FoxO1重组过表达腺病毒的包装与扩繁，在感染牛骨骼肌细胞后，能显著提高FoxO1表达水平（P<0.01）。EdU检测显示过表达FoxO1会显著降低细胞增殖率（P<0.01），流式细胞周期检测显示过表达FoxO1会显著增加G1期细胞数并减少S期和G2期细胞数，抑制细胞G1/S期的转化，并减少G2期细胞的形成。利用qPCR进一步检测发现，增殖相关基因PCNA、CDK1、CDK2、CCNA2、CCNB1、CCND1和CCNE2均极显著下调（P<0.01），促凋亡相关基因BAD和BAX显著上调以及抑凋亡基因BCL2显著下调（P<0.05）。过表达FoxO1导致牛骨骼肌细胞肌管形成量减少，qPCR检测结果发现，骨骼肌分化相关基因MYOD、MYOG、MYF5、MYF6和MYHC的表达量显著下调（P<0.05）。【结论】FoxO1在牛的不同组织中均有表达，是一个广泛存在的转录调控因子，并且在背最长肌组织生长发育不同阶段存在表达差异，起到阶段调控作用。FoxO1在细胞核和细胞质中均发挥重要的转录调控作用，特别是在细胞核内。过表达FoxO1可能通过抑制细胞增殖相关基因和肌细胞分化相关基因的表达，从而抑制牛骨骼肌细胞的增殖与分化，并且可能通过上调促凋亡基因的表达和下调抑凋亡基因的表达来促使牛骨骼肌细胞凋亡的发生。

关键词： FoxO1 牛骨骼肌细胞增殖分化

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Certificateless designated verifier proxy signature scheme for unmanned aerial vehicle networks

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Science China(Information Sciences) 2021年第1期64卷 79-93页

作者： Lei HE Jianfeng MA Limin SHEN dawei wei School of Computer Science and Technology Xidian University School of Computer and Communication Engineering Zhengzhou University of Light Industry School of Cyber Engineering Xidian University School of Computer Science and Technology Nanjing Normal University

Unmanned aerial vehicle(UAV) technologies have a promising application prospect. It is necessary to use digital signature schemes to protect the integrity and authentication of messages in the UAV networks. According to the characteristics of UAV networks, digital signature schemes should solve the problems of digital certificate management and key escrow, guarantee the real-time performance of UAV executing commands, and ensure that only the designated verifier can verify the signature. Therefore, we proposed a certificateless designated verifier proxy signature(CLDVPS) scheme which meets the requirements. We proved our signature scheme is existentially unforgeable under an adaptive chosen message attack in the random oracle model and compared the efficiency of our signature scheme with other signature *** implemented these signature schemes in jPBC to obtain the computing time and theoretically analyzed signature length of these signature schemes. The analysis results indicate that our CLDVPS scheme is more efficient than most of other related signature schemes on computation costs and also efficient on communication costs.

关键词： certificateless designated verifier proxy digital signature unmanned aerial vehicle

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共培养体系下牛骨骼肌细胞对脂肪细胞的调控作用研究

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中国农业科学 2024年第18期57卷 3704-3718页

作者：杨东梅张久盘宋雅萍宋小雨姜超马云魏大为宁夏大学动物科技学院/宁夏回族自治区反刍动物分子细胞育种重点实验室宁夏农林科学院动物科学研究所

【目的】大理石纹是衡量肉品质的重要指标之一，由牛骨骼肌细胞与牛脂肪细胞共同发育形成。通过最大程度模拟体内肉质形成过程中牛骨骼肌细胞和脂肪细胞互作效应，建立体外两种细胞共培养体系，探究共培养体系下牛骨骼肌细胞分泌和代谢... 详细信息

【目的】大理石纹是衡量肉品质的重要指标之一，由牛骨骼肌细胞与牛脂肪细胞共同发育形成。通过最大程度模拟体内肉质形成过程中牛骨骼肌细胞和脂肪细胞互作效应，建立体外两种细胞共培养体系，探究共培养体系下牛骨骼肌细胞分泌和代谢因子对脂肪细胞的调控作用。【方法】采用组织块法和酶消化法分别分离牛脂肪细胞和骨骼肌细胞。利用表型鉴定法和标志基因表达谱鉴定法共同鉴定所分离细胞的纯度和分化潜能，进一步构建牛骨骼肌细胞和牛脂肪细胞条件培养基交换共培养体系以及Transwell共培养体系，通过实时荧光定量PCR(real-time quantitative PCR,qPCR)、EdU染色和油红O染色等方法检测共培养条件下牛骨骼肌细胞分泌和代谢产物对脂肪细胞增殖和分化的影响。【结果】成功构建了两种牛骨骼肌细胞与脂肪细胞的共培养体系。条件培养基交换共培养下，牛脂肪细胞增殖标志基因PCNA、CDK2、CCNE2和CCND1的表达极显著下调（P<0.01）；此外，成脂标志基因FABP4和PPARγ的表达显著下调（P<0.05）;LPL的表达极显著下调（P<0.01）。另一方面，在Transwell共培养条件下，牛脂肪细胞增殖标志基因CCND1的表达显著下调（P<0.05）;PCNA、CDK1和CCNE2的表达极显著下调（P<0.01），且成脂标志基因FABP4的表达显著下调（P<0.05）,PPARγ、C/EBPβ和LPL的表达极显著下调（P<0.01）。【结论】牛骨骼肌细胞分泌和代谢产物可通过抑制脂肪细胞增殖标志基因PCNA、CDK1、CDK2、CCNE2和CCND1以及成脂标志基因PPARγ、FABP4、C/EBPβ和LPL的表达抑制牛脂肪细胞的增殖与分化。以上研究结果可为进一步探究肉牛肌内脂肪沉积的分子机制提供技术支撑。

关键词：牛脂肪细胞骨骼肌细胞共培养细胞互作

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Friction of MoO_(3) Nanoflakes on Graphite Surface with an Ace-like Intercalation Layer

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Chemical Research in Chinese Universities 2022年第3期38卷 769-773页

作者： wei dawei ZHANG Guangjie LU Xiaoquan QIU Xiaohui Tianjin Key Laboratory of Molecular Optoelectronic Department of ChemistrySchool of ScienceTianjin UniversityTianjin300072P.R.China CAS Key Laboratory of Standardization and Measurement for Nanotechnology CAS Center for Excellence in NanoscienceNational Center for Nanoscience and TechnologyBeijing100190P.R.China Key Laboratory of Bioelectrochemistry&Environmental Analysis of Gansu Province College of Chemistry&Chemical EngineeringNorthwest Normal UniversityLanzhou730070P.R.China University of Chinese Academy of Sciences Beijing100049P.R.China

How water layer adsorbed on solid surface under ambient conditions affects the interfacial friction is a fundamental question for understanding the friction and lubrication phenomena in practical *** investigate the formation of ice-like(IL)water layers on the hydrophobic surface of graphite with partially covered MoO_(3)nanoflakes(NFs)using atomic force microscopy(AFM)based *** IL water layers are found surrounding the MoO_(3)NFs and also intercalated at the MoO_(3)/graphite interface,as proved by thickness measurements as well as local adhesion force and surface potential *** manipulations carried out on MoO_(3)NFs on graphite show that the presence of the IL water layers increases the frictional resistance of the *** the results on continuous and discontinuous IL water layers,we can identify the different sliding interfaces in the two *** increased friction for MoO_(3)NFs sliding on graphite with an intercalated water layer is attributed to the energy dissipation originated from the metastable nature of the IL layers.

关键词： Friction Ice-like water MoO_(3) Graphite

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Dataflow Management in the Internet of Things: Sensing,Control, and Security

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Tsinghua Science and Technology 2021年第6期26卷 918-930页

作者： dawei wei Huansheng Ning Feifei Shi Yueliang Wan Jiabo Xu Shunkun Yang Li Zhu School of Computer and Communication Engineering University of Science and Technology BeijingBeijing 100083China Beijing Engineering Research Center for Cyberspace Data Analysis and Applications Beijing 100083China Run Technologies Co. Ltd.Beijing 100192China School of Information Engineering Xinjiang Institute of EngineeringUrumqi 830023China School of Reliability and Systems Engineering Beihang UniversityBeijing 100191China Northern Cloud Research Team Engineering University of PAPXi'an 710086China

The pervasiveness of the smart Internet of Things(IoTs) enables many electric sensors and devices to be connected and generates a large amount of dataflow. Compared with traditional big data, the streaming dataflow is faced with representative challenges, such as high speed, strong variability, rough continuity, and demanding timeliness, which pose severe tests of its efficient management. In this paper, we provide an overall review of IoT dataflow management. We first analyze the key challenges faced with IoT dataflow and initially overview the related techniques in dataflow management, spanning dataflow sensing, mining, control, security, privacy protection,etc. Then, we illustrate and compare representative tools or platforms for IoT dataflow management. In addition,promising application scenarios, such as smart cities, smart transportation, and smart manufacturing, are elaborated,which will provide significant guidance for further research. The management of IoT dataflow is also an important area, which merits in-depth discussions and further study.

关键词： Internet of Things(IoTs) dataflow security privacy management

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FOXO1基因调控牛前体脂肪细胞增殖和分化的功能研究

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中国农业大学学报 2024年第4期29卷 226-238页

作者：宋雅萍张久盘魏大为杨东梅赵毅昂姜超宋小雨吴昊宁夏大学动物科技学院/宁夏反刍动物分子细胞育种重点实验室银川750021 宁夏农林科学院动物科学研究所银川750021

为探究干扰叉头转录因子O1(Forkhead box protein O1, FOXO1)对牛前体脂肪细胞增殖和分化的作用,本研究以固原黄牛的前体脂肪细胞为研究对象,采用组织块法分离牛前体脂肪细胞,并利用表型鉴定和标志基因表达谱2种方法鉴定所分离细胞的纯... 详细信息

为探究干扰叉头转录因子O1(Forkhead box protein O1, FOXO1)对牛前体脂肪细胞增殖和分化的作用,本研究以固原黄牛的前体脂肪细胞为研究对象,采用组织块法分离牛前体脂肪细胞,并利用表型鉴定和标志基因表达谱2种方法鉴定所分离细胞的纯度和分化潜能。筛选并包装牛FOXO1干扰慢病毒,利用EdU染色、流式细胞术、油红O染色和qPCR方法探究干扰FOXO1对牛脂肪细胞增殖和分化的作用。结果表明:1)成功分离了牛前体脂肪细胞,且其具有较高的纯度和分化潜能。2)EdU染色结果表示,侵染Lv-FOXO1极显著增加了牛脂肪细胞的增殖率(P<0.01)。3)流式周期结果显示,干扰FOXO1显著增加了牛脂肪细胞S期阳性细胞的比例,促进了G1/S期的转化,进而促进了牛脂肪细胞增殖。4)干扰FOXO1后,增殖标志基因PCNA、CDK1和CDK2的表达量均极显著上调(P<0.01)。5)干扰FOXO1后牛前体脂肪细胞脂滴生成明显减少,且成脂标志基因PPARG的表达极显著下调(P<0.01),CEBPB和LPL的表达显著下调(P<0.05)。综上,干扰FOXO1可通过上调增殖标志基因PCNA、CDK1和CDK2的表达促进牛脂肪细胞G1/S期的转化,进而促进牛脂肪细胞增殖,且其可通过降低成脂标志基因PPARG、CEBPB和LPL的表达减少牛脂肪细胞脂滴形成,进而影响脂肪沉积。本研究可为中国地方黄牛的肉质遗传改良提供理论依据。

关键词： FOXO1 固原黄牛前体脂肪细胞增殖分化

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染色质开放状态对食管癌相关通路影响及关键基因分析

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长治医学院学报 2024年第2期38卷 81-86页

作者：申展铭李向东魏大为张文平长治医学院山西长治046000 长治医学院附属和平医院胸外科

目的:探讨染色质开放状态对食管癌相关通路的影响及对关键基因进行分析。方法:从癌症基因组图谱(TCGA)数据库下载食管癌染色质开放性高通量测序(ATAC-seq)数据,使用R4.2.1对2组数据分别进行基因注释,并进行KEGG通路富集分析与GO功能富... 详细信息

目的:探讨染色质开放状态对食管癌相关通路的影响及对关键基因进行分析。方法:从癌症基因组图谱(TCGA)数据库下载食管癌染色质开放性高通量测序(ATAC-seq)数据,使用R4.2.1对2组数据分别进行基因注释,并进行KEGG通路富集分析与GO功能富集分析;对食管癌RNA-seq数据进行差异分析,筛选出与染色质开放状态相关性较高的2个基因ARL5B和RUNX1,并绘制KM生存曲线;对ARL5B、RUNX1进行单因素与多因素COX分析。结果:食管癌中ATAC-seq数据的峰值距离转录起始位点≤1 kb、1~2 kb、2~3 kb所占百分比分别为32.59%、6%、4.41%,位于远端基因间区的峰值占27.15%,这种现象和染色质开放区域的分布相一致。染色质开放区峰值绝大多数存在于转录起始位点附近,符合染色质开放性的特点。KEGG和GO功能分析结果显示,注释基因在Rap1信号通路、Hippo信号通路、ErbB信号通路、mTOR信号通路等明显富集。ARL5B高表达的食管癌患者预后较差(P<0.05),RUNX1高表达的食管癌患者预后较好(P<0.05)。结论:染色质开放状态在调节食管癌的发生和发展方面发挥重要作用,ARL5B、RUNX1基因可能成为食管癌的治疗靶点和预后指标。

关键词：食管癌 TCGA数据库 ATAC-seq 染色体可及性

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Non-intrusive Load Monitoring Based on Graph Total Variation for Residential Appliances

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Journal of Modern Power Systems and Clean Energy 2024年第3期12卷 947-957页

作者： Xiaoyang Ma Diwen Zheng Xiaoyong Deng Ying Wang dawei Deng wei Li the College of Electrical Engineering Sichuan UniversityChengdu 610065China State Grid Chongqing Electric Power Company Chongqing 400014China State Grid Sichuan Electric Power Company Chengdu 610095China State Grid Hubei Electric Power Company Limited Research Institute Wuhan 430077China

Non-intrusive load monitoring is a technique for monitoring the operating conditions of electrical appliances by collecting the aggregated electrical information at the household power *** several studies on the mining of unique load characteristics,few studies have extensively considered the high computational burden and sample *** on lowfrequency sampling data,a non-intrusive load monitoring algorithm utilizing the graph total variation(GTV)is proposed in this *** algorithm can effectively depict the load state without the need for prior ***,the combined Kmeans clustering algorithm and graph signals are used to build concise and accurate graph structures as load *** GTV representing the internal structure of the graph signal is introduced as the optimization model and solved using the augmented Lagrangian iterative *** introduction of the difference operator reduces the computing cost and addresses the inaccurate reconstruction of the graph *** low-frequency sampling data,the algorithm only requires a little prior data and no training,thereby reducing the computing *** conducted using the reference energy disaggregation dataset and almanac of minutely power dataset demonstrated the stable superiority of the algorithm and its low computational burden.

关键词： Non-intrusive load monitoring graph total variation augmented Lagrangian function smart grid

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