Understanding the rapid spread of antimicrobial resistance genes mediated by IS26

作     者：Na Tang Dawei Wei Yuan Zeng Gang Zhang Chao Wang Jie Feng Yuqin Song 

作者机构：State Key Laboratory of Microbial ResourcesInstitute of MicrobiologyChinese Academy of SciencesBejingChina College of Life ScienceUniversity of Chinese Academy of SciencesBeijingChina 

出 版 物：《mLife》 (微生物（英文）)

年 卷 期：2024年第3卷第1期

页      面：101-109页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 071005[理学-微生物学] 10[医学] 

基　　金：supported by the National Key R&D Program of China(grant No.2021YFC2302005) the National Natural Science Foundation of China(grant Nos.32300162 and 32070075) 

主　　题：antibiotic consumption antibiotic resistance genes horizontal gene transfer insertion sequence IS26 

摘      要：Insertion sequences(ISs)promote the transmission of antimicrobial resistance genes(ARGs)across bacterial ***,their contributions and dynamics during the transmission of resistance remain *** this study,we selected is26 as a representative transposable element to decipher the relationship between ISs and ARGs and to investigate their transfer features and transmission *** retrieved 2656 translocatable IS26-bounded units with ARGs(tiS26-bUs-ARGs)in complete bacterial genomes from the NCBI RefSeq *** total,124 ARGs spanning 12 classes of antibiotics were detected,and the average contribution rate of IS26 to these genes was 41.2%.We found that IS26-bounded units(IS26-bUs)mediated extensive ARG dissemination within the bacteria of the Gammaproteobacteria class,showing strong transfer po-tential between strains,species,and even *** Is26-bUs expanded in bacterial populations over time,and their temporal expansion trend was significantly correlated with antibiotic *** wide dissemination could be due to the nonspecific target site preference of ***,we experimentally confirmed that the introduction of a single copy of is26 could lead to the formation of a composite transposon mediating the transmission of“passenger*** observations extend our knowledge of the IS26 and provide new insights into the mediating role of ISs in the dissemination of antibiotic resistance.