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Effect Found in the Combination of Kitasamycin with Other Antibiotics Used against Actinobacillus pleuropneumoniae

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Journal of Biosciences and Medicines 2020年第7期8卷 63-75页

作者： Elsa Patricia Flores-Castellanos Sofía González Gallardo Jose Ivan Sanchez-Betancourt Maria Elena Trujillo Ortega david quintanar guerrero Abel Ciprian Carrasco Susana E. Mendoza Elvira Facultad de Estudios Superiores Cuautitlán (FESC) Universidad Nacional Autónoma de México México City México Facultad de Medicina Veterinaria y Zootecnia (FMVZ) Universidad Nacional Autónoma de México México City México

Background: Combinations of antibiotics are used to increase the therapeutic options via the simultaneous activities of two compounds. The goals of combination therapy are to resolve the bacterial infection while reducing the treatment cost. The use of kitasamycin is used frequently and in addition to other antibiotics, the desired combinations were not found. Methods: Fields strains: we use serotypes 1, 3, 5 y 7. Microbial culturing: The serotypes 1, 3, 5 y 7 was identified. Antibiotics and Reagents: The antibiotics used were kitasamycin;enrofloxacin and oxolinic acid. The following combinations were tested: a) kitasamycin with enrofloxacin;b) kitasamycin with norfloxacin;and c) kitasamycin with oxolinic acid. Preparation of bacterial suspensions: The cultures were adjusted to a concentration of 5 colony-forming units per ml (CFU/ml) using the same sterile culture medium as the diluent. Determination of the fractional inhibitory concentrations (FICs): To test each combination and their interactions and to calculate the fractional inhibitory concentration (FIC) of each antibiotic. Calculation of the index of the fractional minimum inhibitory concentration (FMIC): The FMIC index was calculated by applying the following formula: FIC index = FIC of antibiotic A/FIC of antibiotic B. Results: What was found showed that: A combination of kitasamycin and norfloxacin has been shown to have an indifferent effect. A combination of kitasamycin and enrofloxacin had antagonistic effects against all of the Actinobacillus pleuropneumoniae serotypes studied. Finally, a combination of kitasamycin and oxolinic acid had antagonist effects against serotypes 1, 3 and 5 and a synergistic effect against serotype 7. Conclusion: The “in vitro” combination Kitasamycin-Norfloxacin showed an indifferent effect, and combination Kitasamycin and Enrofloxacin demonstrated an antagonistic interaction between these two anti

关键词： Antibiotics Combination A. pleuropneumoniae

Reverse Droop Control-based Smooth Transfer Strategy for Interface Converters in Hybrid AC/DC Distribution Networks

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CSEE Journal of Power and Energy Systems 2023年第1期9卷 122-134页

作者： Wenyuan Cao Minxiao Han Xiangkun Meng Wenqiang Xie Zmarrak Wali Khan Josep M.guerrero Gibran david Agundis Tinajero School of Electric and Electronic Engineering North China Electric Power UniversityBeijing 102206China Department of Energy Technology Aalborg UniversityAalborg 9220Denmark

Hybrid AC/DC distribution networks are promising candidates for future applications due to their rapid advancement in power electronics *** use interface converters(IFCs)to link DC and AC distribution ***,the networks possess drawbacks with AC voltage and frequency offsets when transferring from grid-tied to islanding *** address these problems,this paper proposes a simple but effective strategy based on the reverse droop ***,the power balance equation of the distribution system is derived,which reveals that the cause of voltage and frequency offsets is the mismatch between the IFC output power and the rated load ***,the reverse droop control is introduced into the IFC *** using a voltage-active power/frequency-reactive power(U-P/f-Q)reverse droop loop,the IFC output power enables adaptive tracking of the rated load ***,the AC voltage offset and frequency offset are suppressed during the transfer process of operational *** addition,the universal parameter design method is discussed based on the stability limitations of the control system and the voltage quality requirements of AC critical ***,simulation and experimental results clearly validate the proposed control strategy and parameter design method.

关键词： Adaptive adjustment hybrid AC/DC distribution network interface converters parameter design method reverse droop control

Fat grafting in autologous breast reconstruction:applications,outcomes,safety,and complications

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Plastic and Aesthetic Research 2023年第1期10卷 485-500页

作者： Bahaa Shaaban david guerrero Carolyn De La Cruz Lauren Kokai Department of Plastic Surgery University of PittsburghPittsburghPA 15213USA University of Pittsburgh Medical Center PittsburghPA 15213USA Department of Bioengineering University of PittsburghPittsburghPA 15213USA McGowan Institute for Regenerative Medicine PittsburghPA 15213USA

Autologous fat grafting is an important surgical technique in aesthetic and reconstructive *** grafting for breast reconstruction is now an established procedure for adding volume and improving cutaneous pliability;it can be used independently to replace more invasive flap procedures or implants,or as an adjunct for smaller volume *** breadth of applications in the breast necessitates diversity in technique and approach,and while there is no universally agreed-upon protocol,basic principles have guided the evolution of some commonly adopted ***,fat grafting outcomes are highly favorable but dependent on patient and procedure factors,requiring learned patient selection and expertise in recipient site *** complications from fat grafting,such as fat necrosis and the development of nodules,are particularly troublesome for post-oncologic patients,requiring considerable pre-surgical consultation for patient education and managing *** addition to volume and contour augmentation,fat grafting has additional beneficial effects that have recently drawn increased attention including pain reduction from implant capsular contracture or postmastectomy pain syndrome,improved skin quality and reduced fibrosis following radiation,and possible anti-tumorigenic *** developments in clinical fat grafting research that are promising include the use of adipose progenitor cells admixed with lipoaspirate for improved volume retention or alternative biologics such as platelet-rich ***,research towards safe and effective regenerative medicine approaches is actively underway,with the ultimate goal of achieving predictable and increased graft retention,reducing the number of required surgical procedures and enabling on-table results to reflect procedure outcomes.

关键词： Fat grafting fat transfer autologous breast reconstruction implant reconstruction lipo-injection breast augmentation graft survival skin expansion mastectomy post-mastectomy pain breast fibrosis capsular contracture

Liver transplantation is beneficial regardless of cirrhosis stage or acute-on-chronic liver failure grade:A single-center experience

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World Journal of Gastroenterology 2022年第40期28卷 5881-5892页

作者： Eduardo Cervantes-Alvarez Mario Vilatoba Nathaly Limon-de la Rosa Osvely Mendez-guerrero david Kershenobich Aldo Torre Nalu Navarro-Alvarez PECEM Facultad de MedicinaUniversidad Nacional Autónoma de MéxicoMexico City 14080Mexico Department of Gastroenterology Instituto Nacional de Ciencias Médicas y Nutrición Salvador ZubiránMexico City 14080Mexico Department of Trasplant Instituto Nacional de Ciencias Médicas y Nutrición Salvador ZubiránMexico City 14080Mexico Department of Molecular Biology Universidad Panamericana School of MedicineMexico City 03920Mexico Department of Surgery University of Colorado Anschutz Medical CampusDenverCO 80045United States

BACKGROUND Liver transplantation for the most critically ill remains controversial;however,it is currently the only curative treatment *** To assess immediate posttransplant outcomes and compare the short(1 year)and long-term(6 years)posttransplant survival among cirrhotic patients stratified by disease *** We included cirrhotic patients undergoing liver transplantation between 2015 and 2019 and categorized them into compensated cirrhosis(CC),decompensated cirrhosis(DC),and acute-on-chronic liver failure(ACLF).ACLF was further divided into severity *** primary outcomes of interest were total days of intensive care unit(ICU)and hospital stay,development of complications and posttransplant survival at 1 and 6 *** 235 patients underwent liver transplantation(CC=11,DC=129 and ACLF=95).Patients with ACLF had a significantly longer hospital stay[8.0(6.0-13.0)vs CC,6.0(3.0-7.0),and DC 7.0(4.5-10.0);P=0.01]and developed more infection-related complications[47(49.5%),vs CC,1(9.1%)and DC,38(29.5%);P<0.01].Posttransplant survival at 1-and 6-years was similar among groups(P=0.60 and P=0.90,respectively).ACLF patients stratified according to ACLF grade[ACLF-1 n=40(42.1%),ACLF-2 n=33(34.7%)and ACLF-3 n=22(23.2%)],had similar ICU and hospital stay length(P=0.68,P=0.54),as well as comparable frequencies of overall and infectious posttransplant complications(P=0.58,P=0.80).There was no survival difference between ACLF grades at 1 year and 6 years(P=0.40 and P=0.15).CONCLUSION Patients may benefit from liver transplantation regardless of the cirrhosis *** patients have a longer hospital stay and frequency of infectious complications;however,excellent,and comparable 1 and 6-year survival rates support their enlisting and transplantation including those with ACLF-3.

关键词： Liver transplantation Acute-on-chronic liver failure Prognosis Survival analysis Critical care

Recommendations for the use of the acetaminophen hepatotoxicity model for mechanistic studies and how to avoid common pitfalls

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Acta Pharmaceutica Sinica B 2021年第12期11卷 3740-3755页

作者： Hartmut Jaeschke Olamide B.Adelusi Jephte Y.Akakpo Nga T.Nguyen Giselle Sanchez-guerrero david S.Umbaugh Wen-Xing Ding Anup Ramachandran Department of Pharmacology Toxicology&TherapeuticsUniversity of Kansas Medical CenterKansas CityKS 66160USA

Acetaminophen(APAP)is a widely used analgesic and antipyretic drug,which is safe at therapeutic doses but can cause severe liver injury and even liver failure after *** mouse model of APAP hepatotoxicity recapitulates closely the human *** a result,this clinically relevant model is frequently used to study mechanisms of drug-induced liver injury and even more so to test potential therapeutic ***,the complexity of the model requires a thorough understanding of the pathophysiology to obtain valid results and mechanistic information that is translatable to the ***,many studies using this model are flawed,which jeopardizes the scientific and clinical *** purpose of this review is to provide a framework of the model where mechanistically sound and clinically relevant data can be *** discussion provides insight into the injury mechanisms and how to study it including the critical roles of drug metabolism,mitochondrial dysfunction,necrotic cell death,autophagy and the sterile inflammatory *** addition,the most frequently made mistakes when using this model are ***,considering these recommendations when studying APAP hepatotoxicity will facilitate the discovery of more clinically relevant interventions.

关键词： Acetaminophen hepatotoxicity Drug metabolism Mitochondria Apoptosis Ferroptosis Autophagy NRF2 Innate immunity

Cholinergic neuromuscular junctions in Brachionus calyciflorus and Lecane quadridentata(Rotifera:Monogononta)

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Journal of Coastal Life Medicine 2014年第5期 376-381页

作者： Ignacio Alejandro Pérez-Legaspi Alma Lilián guerrero-Barrera Iván JoséGalván-Mendoza JoséLuis quintanar Roberto Rico-Martínez Graduate Studies and Research Division Boca del Río Institute of TechnologyKm.12 Carr.Veracruz–Córdoba94290Boca del RíoVer.México Morphology Department Basic Sciences CenterAguascalientes Autonomous UniversityAve.Universidad 940Aguascalientes20100México Confocal Microscopy Unit Experimental Services DepartmentCINVESTAV-ZacatencoNational Politechnic InstituteAve.Politécnico2508Zacatenco56000MéxicoD.F.México Pharmacology and Physiology Department Aguascalientes Autonomous UniversityAve.Universidad 940Aguascalientes20100México Chemistry Department Basic Sciences CenterAguascalientes Autonomous UniversityAve.Universidad 940Aguascalientes20100México

Objective:To identify the presence of joint muscular and cholinergic systems in two freshwater rotifer species,Brachionus calyciflorus and Lecane ***:The muscle actin fibers were stained with phalloidin-linked fluorescent dye,and acetylcholine was detected with Amplex Red Acetylcholine/Acetylcholinesterase Assay Kit,and then confocal scanning laser microscopy was ***:The musculature of Brachionus calyciflorus showed a pattern similar to other species of the same genus,while that of Lecane quadridentata was different from other rotifer genera described *** cholinergic system was determined by co-localization of both muscles and acetylcholine labels in the whole rotifer,suggesting the presence of neuromuscular ***:The distribution pattern of muscular and acetylcholine systems showed considerable differences between the two species that might be related to different adaptations to particular ecological *** confirmation of a cholinergic system in rotifers contributes to the development of potential neuro-pharmacological and toxicological studies using rotifers as model organism.

关键词： Acetylcholine Co-localization Confocal microscopy F-actin Rotifer

Neuroprotective effects of G9a inhibition through modulation of peroxisome-proliferator activator receptor gamma-dependent pathways by miR-128

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Neural Regeneration Research 2024年第11期19卷 2532-2542页

作者： Aina Bellver-Sanchis Pedro AAvila-López Iva Tic david Valle-García Marta Ribalta-Vilella Luis Labrador Deb Ranjan Banerjee Ana guerrero Gemma Casadesus Coralie Poulard Mercè Pallàs Christian Grinán-Ferré Department of Pharmacology and Therapeutic Chemistry Institut de Neurociències-Universitat de BarcelonaBarcelonaSpain Department of Biochemistry and Molecular Genetics Feinberg School of MedicineNorthwestern UniversityChicagoILUSA Institute of Biotechnology National Autonomous University of MexicoCuernavacaMexico Department of Pharmacology and Therapeutics Health Science Center-University of FloridaGainesvilleFLUSA Department of Chemistry National Institute of Technology DurgapurM G AvenueDurgapurWest BengalIndia Cancer Research Cancer Lyon Universitéde LyonLyonFrance Inserm U1052 Centre de Recherche en Cancérologie de LyonLyonFrance CNRS UMR5286 Centre de Recherche en Cancérlogie de LyonLyonFrance Centro de Investigación en Red Enfermedades Neurodegenerativas(CIBERNED)Instituto de Salud Carlos IIIMadridSpain Neuroimmunology Department National Institute of Neurology and Neurosurgery“Manuel Velasco Suárez”Mexico CityMexico

Dysregulation of G9a,a histone-lysine N-methyltransferase,has been observed in Alzheimer’s disease and has been correlated with increased levels of chronic inflammation and oxidative ***,microRNAs are involved in many biological processes and diseases playing a key role in pathogenesis,especially in multifactorial diseases such as Alzheimer’s ***,our aim has been to provide partial insights into the interconnection between G9a,microRNAs,oxidative stress,and *** better understand the biology of G9a,we compared the global microRNA expression between senescence-accelerated mouse-prone 8(SAMP8)control mice and SAMP8 treated with G9a inhibitor *** found a downregulation of miR-128 after a G9a inhibition treatment,which interestingly binds to the 3′untranslated region(3′-UTR)of peroxisome-proliferator activator receptor γ(PPARG)***,Pparg gene expression levels were higher in the SAMP8 group treated with G9a inhibitor than in the SAMP8 control *** also observed modulation of oxidative stress responses might be mainly driven Pparg after G9a *** confirm these antioxidant effects,we treated primary neuron cell cultures with hydrogen peroxide as an oxidative *** this setting,treatment with G9a inhibitor increases both cell survival and antioxidant ***,up-regulation of PPARγby G9a inhibitor could also increase the expression of genes involved in DNA damage responses and *** addition,we also described that the PPARγ/AMPK axis partially explains the regulation of autophagy markers ***,PPARγ/GADD45αpotentially contributes to enhancing synaptic plasticity and neurogenesis after G9a ***,we propose that pharmacological inhibition of G9a leads to a neuroprotective effect that could be due,at least in part,by the modulation of PPARγ-dependent pathways by miR-128.

关键词： aging cognitive decline epigenetics G9a inhibition microRNAs miR-128 peroxisome-proliferator activator receptorγ(PPARγ) PPARG SAMP8

Glycyrrhizic Acid-Loaded Poly-ɛ-Caprolactone Nanoparticles Decrease PRRSV Infection in MARC-145 Cells

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Open Journal of Veterinary Medicine 2023年第12期13卷 221-236页

作者： Samantha Jardon Oscar Escalona Carlos Gerardo García david quintanar Carlos Ignacio Soto María Zaida Urbán Susana Elisa Mendoza Multidisciplinary Research Unit L4 (Veterinary Morphology and Cell Biology) UNAM-FESC Campus 4 Cuautitlán Izcalli Mexico Pharmaceutical Technology Research Laboratory UNAM-FESC Campus 1 Cuautitlán Izcalli Mexico Dr. Hideyo Noguchi Regional Research Center UAY Mérida Mexico Laboratory of Virology and Microbiology of Respiratory Diseases of The Pig UNAM-FESC Campus 1 Cuautitlán Izcalli Mexico

Porcine reproductive and respiratory syndrome (PRRS) is an economically devastating disease with worldwide distribution caused by Betaarterivirus suid (PRRSV). The virion has great genetic and antigenic variability with a marked increase in virulence. Vaccines tested to date have been of little use in controlling the problems caused by PRRSV, so the present study was conceived to evaluate the antiviral effect of polymeric nanoparticles (PNPs) made with glycyrrhizic acid (GA). Recent work has proven that this nanoparticle system is stable. These nanoparticles have good GA carrying capacity, a size < 250 nm, a spherical morphology, and a wide safety range. The integrity of cell morphology can be maintained for up to 72 h. The antiviral effect of this nanoparticle system was tested in cultures of MARC-145 cells in pre- and coinfection assays with PRRSV to evaluate changes in cell morphology and effects on cell viability. The use of PNPsGA with the real-time quantitative polymerase chain reaction (RT-qPCR) decreased viral infection by 38% in 3 amplification cycles. These results suggest that this system has an antiviral effect against PRRSV under the study conditions established.

关键词： Cytotoxicity Glycyrrhizic Acid Cell Morphology Polymeric Nanoparticles PRRS Virus

Fiber Residues from Canavalia ensiformisL. Seeds with Potential Use in Food Industry

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Agricultural Sciences 2014年第13期5卷 1227-1236页

作者： Maira Rubi Segura-Campos Lourdes Manrique-Reynoso Luis Chel-guerrero david Betancur-Ancona Faculty of Chemical Engineering Autonomous University of Yucatán Merida Mexico

The objective of this research was to determine the physicochemical characteristics of fiber residues from Jack bean (Canavalia ensiformis L.) obtained by two technological processes. The proximal composition of the fiber residues from Canavalia ensiformis registered values of moisture, ash, protein, fat, fiber and nitrogen-free extract (NFE) of 7.14%, 3.17%, 9.14%, 1.34%, ?23.84% and 62.51% for residue A and 4.74%, 2.68%, 7.73%, 1.39%, 23.76% and 64.44% for residue B. Total dietary fiber (TDF) contents in the fiber residues were 47.06 (Residue A) and 54.96 (Residue B) g/100g sample, with most of this content represented by insoluble dietary fiber (IDF) 45.46 g/100g sample in Residue A and 52.75 g/100g of sample in Residue B. The remainder was constituted by soluble dietary fiber (SDF). The neutral detergent fiber (NDF) content was slightly higher in residue B (41.8 g/100g sample). Acid detergent fiber (ADF) that includes principally cellulose, lignin and cutin, and acid detergent lignin (ADL) that include lignin and cutin were higher in residue B (32.5 g/100g sample) and similar for both residues (1.0 (A) and 1.2 (B) g/100g sample), respectively. Resistant starch (RS) was higher in residue B (0.607%) than in residue A (0.358%). No statistical difference (p > 0.05) was registered in the tannins content of both residues. However, the phytates content was higher in the fiber residue obtained by the fists technological process (A residue). In vitro digestibility was higher in residue A (85.81%) than that in B residue (81.51%). The results of the present study suggest the potential use of C. ensiformis fiber residues as a functional ingredient in foods, especially in the development of reduced calorie food and dietary fiber enriched foods.

关键词： C. ensiformis Jack Bean Dietary Fiber Chemical Characterization In Vitro Digestibility