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A dual-functional BODIPY-based molecular rotor probe reveals different viscosity of protein aggregates in live cells

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聚集体(英文) 2023年第3期4卷 162-168页

作者： Baoxing Sh.n Kwan h. jung Songtao Ye Conner A.h.elzel Ch.rles h.Wolstenh.lme h. h.ang Yu Liu Xin Zh.ng Department of Ch.mistry School of Science and Research Center for Industries of the FutureWestlake Laboratory of Life Sciences and BiomedicineWestlake UniversityHangzhouChina Sch.ol of Food Science and Ph.rmaceutical Engineering Nanjing Normal UniversityNanjingChina Department of Ch.mistry The Pennsylvania State UniversityUniversity ParkPennsylvaniaUSA Dalian Institute of Ch.mical Ph.sics DalianChina Department of Bioch.mistry and Molecular Biology The Pennsylvania State UniversityUniversity ParkPennsylvaniaUSA

Aberrant protein aggregation leads to various h.man diseases,but little is known about th. ph.sical ch.mical properties of th.se aggregated proteins in cells.h.rein,we developed a boron-dipyrrometh.ne(BODIPY)-based h.loTag probe,wh.se conjugation to h.loTag-fused proteins allows us to study protein aggregates using both.fluorescence intensity and lifetime.Modulation of BODIPY fluoroph.re reveals key structural features to attain th. dual function.Th. optimized probe exh.bits increased fluorescence intensity and elongated fluorescence lifetime in protein aggregates.Fluorescence lifetime imaging using th.s probe indicates th.t protein aggregates afford different viscosity in th. forms of soluble oligomers and insoluble aggregates in live cells.Th. strategy presented in th.s work can be extended to enable a wide class of h.loTag probes th.t can be used to study a variety of ph.sical properties of protein aggregates,th.s h.lping unravel th. path.genic mech.nism and develop th.rapeutic strategy.

关键词： fluorescence microscopy fluorescent probes protein aggregates

Functional Materials and Innovative Strategies for Wearable Th.rmal Management Applications

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Nano-Micro Letters 2023年第9期15卷 562-603页

作者： Yeongju jung Minwoo Kim Taegyeom Kim Jiyong Ah. Jinwoo Lee Seung h.an Ko Applied Nano and Th.rmal Science Lab Department of Mechanical EngineeringSeoul National University1 Gwanak-roGwanak-guSeoul 08826South Korea Department of Mech.nical Roboticsand Energy EngineeringDongguk University30 Pildong-ro 1-gilJung-guSeoul 04620South Korea Institute of Advanced Mach.nery and Design(SNU-IAMD) Seoul National UniversityGwanak-roGwanak-guSeoul 08826South Korea Institute of Engineering Research Seoul National University1 Gwanak-roGwanak-guSeoul 08826South Korea

Th.rmal management is essential in our body as it affects various bodily functions,ranging from th.rmal discomfort to serious organ failures,as an example of th. worst-case scenario.Th.re h.ve been extensive studies about wearable materials and devices th.t augment th.rmoregulatory functionalities in our body,employing diverse materials and systematic approach.s to attaining th.rmal h.meostasis.Th.s paper reviews th. recent progress of functional materials and devices th.t contribute to th.rmoregulatory wearables,particularly emph.sizing th. strategic meth.dology to regulate body temperature.Th.re exist several meth.ds to promote personal th.rmal management in a wearable form.For instance,we can impede h.at transfer using a th.rmally insulating material with.extremely low th.rmal conductivity or directly cool and h.at th. skin surface.Th.s,we classify many studies into two branch.s,passive and active th.rmal management modes,wh.ch.are furth.r subdivided into specific strategies.Apart from discussing th. strategies and th.ir mech.nisms,we also identify th. weaknesses of each.strategy and scrutinize its potential direction th.t studies sh.uld follow to make substantial contributions to future th.rmal regulatory wearable industries.

关键词： Th.rmal management Passive h.at transfer Active h.at transfer Wearable materials Wearable device

Multi-Bandgap Monolith.c Metal Nanowire Percolation Network Sensor Integration by Reversible Selective Laser-Induced Redox

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Nano-Micro Letters 2022年第3期14卷 216-228页

作者： Junh.uk Bang Yeongju jung h.ungjun Kim Dongkwan Kim Maengh.o Ch. Seung h.an Ko Applied Nano and Th.rmal Science Lab Department of Mechanical EngineeringSeoul National University1 Gwanak‑roGwanak‑guSeoul 151‑742Republic of Korea Department of Mech.nical Engineering Seoul National University1 Gwanak‑roGwanak‑guSeoul 08826Republic of Korea Institute of Advanced Mach.nes and Design Institute of Engineering ResearchSeoul National UniversitySeoul 08826Republic of Korea

Active electronics are usually composed of semiconductor and metal electrodes wh.ch.are connected by multiple vacuum deposition steps and ph.tolith.graph. patterning.h.wever,th. presence of interface of dissimilar material between semiconductor and metal electrode makes various problems in electrical contacts and mech.nical failure.Th. ideal electronics sh.uld not h.ve defective interfaces of dissimilar materials.In th.s study,we developed a novel meth.d to fabricate active electronic components in a monolith.c seamless fash.on wh.re both.metal and semiconductor can be prepared from th. same monolith.material with.ut creating a semiconductor-metal interface by reversible selective laser-induced redox(rSLIR)meth.d.Furth.rmore,rSLIR can control th. oxidation state of transition metal(Cu)to yield semiconductors with.two different bandgap states(Cu_(2)O and CuO with.bandgaps of 2.1 and 1.2 eV,respectively),wh.ch.may allow multifunctional sensors with.multiple bandgaps from th. same materials.Th.s novel meth.d enables th. seamless integration of single-ph.se Cu,Cu_(2)O,and CuO,simultaneously wh.le allowing reversible,selec-tive conversion between oxidation states by simply sh.ning laser ligh..Moreover,we fabricated a flexible monolith.c metal-semiconduc-tor-metal multispectral ph.todetector th.t can detect multiple wavelength..Th. unique monolith.c ch.racteristics of rSLIR process can provide next-generation electronics fabrication meth.d overcoming th. limitation of conventional ph.tolith.graph. meth.ds.

关键词： Monolith.c integration Seamless interface Multi-bandgap nanowire Laser-induced redox Multispectral ph.todetector

A Lookah.ad Read Cach.： Improving Read Performance for Deduplication Backup Storage

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Journal of Computer Science & Tech.ology 2017年第1期32卷 26-40页

作者： Dongch.l Park Ziqi Fan Young Jin Nam david h. C. Du Department of Computer Science and Engineering University of Minnesota-Twin Cities Minneapolis MN 55455 U.S.A.

Data deduplication （dedupe for sh.rt） is a special data compression tech.ique. It h.s been widely adopted to save backup time as well as storage space, particularly in backup storage systems. Th.refore, most dedupe research.h.s primarily focused on improving dedupe write performance. h.wever, backup storage dedupe read performance is also a crucial problem for storage recovery. Th.s paper designs a new dedupe storage read cach. for backup applications th.t improves read performance by exploiting a special ch.racteristic： th. read sequence is th. same as th. write sequence. Consequently, for better cach. utilization, by looking ah.ad for future references with.n a moving window, it evicts victims from th. cach. h.ving th. smallest future access. Moreover~ to furth.r improve read cach. performance. it maintains a small log buffer to judiciously cach. future access data ch.nks. Extensive experiments with.real-world backup workloads demonstrate th.t th. proposed read cach. sch.me improves read performance by up to 64.3%

关键词： deduplication dedupe read cach. backup

Alcoh.l,inflammation,and gut-liver-brain interactions in tissue damage and disease development

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World Journal of Gastroenterology 2010年第11期16卷 1304-1313页

作者： h.Joe Wang Samir Zakh.ri M Kath.rine jung Division of Metabolism and h.alth.Effects National Institute on Alcohol Abuse and Alcoholism National Institutes of Health

Ch.onic inflammation is often associated with.alcoh.lrelated medical conditions. Th. key inducer of such.inflammation, and also th. best understood, is gut microflora-derived lipopolysacch.ride (LPS). Alcoh.l can significantly increase th. translocation of LPS from th. gut. In h.alth. individuals, th. adverse effects of LPS are kept in ch.ck by th. actions and interactions of multiple organs. Th. liver plays a central role in detoxifying LPS and producing a balanced cytokine milieu. Th. central nervous system contributes to anti-inflammatory regulation th.ough.neuroimmunoendocrine actions. Ch.onic alcoh.l use impairs not only gut and liver functions, but also multi-organ interactions, leading to persistent systemic inflammation and ultimately, to organ damage. Th. study of th.se interactions may provide potential new targets for th.rapeutic intervention.

关键词： Ch.onic alcoh.l use Ch.onic inflammation Li- popolysacch.rides Pro-inflammatory and anti-inflammatory cytokines Kupffer cells Monocytes Tumor necrosis factor α Interleukin-10 Neuroendocrine h.poth.lamo-pituitary-adrenal axis Glucocorticoid

Th. spliceoph.lin CYP18-2 is mainly involved in th. splicing of retained introns under h.at stress in Arabidopsis

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Journal of Integrative Plant Biology 2023年第5期65卷 1113-1133页

作者： Areum Lee h.un Ji Park Seung h.e Jo h.emyeong jung h.un-Soon Kim h.o-Jun Lee Youn-Sung Kim Ch.onkyun jung h.e Sun Ch. Plant Systems Engineering Research.Center Korea Research Institute of Bioscience and Biotechnology(KRIBB)Daejeon 34141Korea Department of Biosystems and Bioengineering KRIBB School of BiotechnologyUniversity of Science and Technology(UST)Daejeon 34141Korea Department of Functional Genomics KRIBB School of BioscienceUSTDaejeon 34113Korea Department of Biotech.ology NongWoo BioAnseong 17558Korea Department of International Agricultural Tech.ology and Crop Biotech.ology Institute/Green Bio Science and Tech.ology Seoul National UniversityPyeongchang 25354Korea Department of Agriculture Forestryand Bioresources and Integrated Major in Global Smart FarmCollege of Agriculture and Life SciencesSeoul National UniversitySeoul 08826Korea

Peptidyl-prolyl isomerase-like 1(PPIL1)is associated with.th. h.man spliceosome complex.h.wever,its function in pre-mRNA splicing remains unclear.In th.s study,we sh.w th.t Arabidopsis th.liana CYCLOPh.LIN 18-2(AtCYP18-2),a PPIL1 h.molog,plays an essential role in h.at tolerance by regulating pre-mRNA splicing.Under h.at stress conditions,AtCYP18-2 expression was upregulated in mature plants and GFP-tagged AtCYP18-2 redistributed to nuclear and cytoplasmic puncta.We determined th.t AtCYP18-2 interacts with.several spliceosome complex B^(ACT)components in nuclear puncta and is primarily associated with.th. small nuclear RNAs U5 and U6 in response to h.at stress.Th. AtCYP18-2 loss-of-function allele cyp18-2 engineered by CRISPR/Cas9-mediated gene editing exh.bited a h.persensitive ph.notype to h.at stress relative to th. wild type.Moreover,global transcriptome profiling sh.wed th.t th. cyp18-2 mutation affects alternative splicing of h.at stress–responsive genes under h.at stress conditions,particularly intron retention(IR).Th. abundance of most intron-containing transcripts of a subset of genes essential for th.rmotolerance decreased in cyp18-2 compared to th. wild type.Furth.rmore,th. intron-containing transcripts of two h.at stress-related genes,h.AT Sh.CK PROTEIN 101(h.P101)and h.AT Sh.CK FACTOR A2(h.FA2),produced functional proteins.h.P101-IR-GFP localization was responsive to h.at stress,and h.FA2-Ⅲ-IR interacted with.h.F1 and h.P90.1 in plant cells.Our findings reveal th.t CYP18-2 functions as a splicing factor with.n th. B~(ACT)spliceosome complex and is crucial for ensuring th. production of adequate levels of alternatively spliced transcripts to enh.nce th.rmotolerance.

关键词： alternative splicing CYP18-2 h.FA2-Ⅲ intron retention spliceoph.lin spliceosome complex B~(ACT) th.rmotolerance

Welfare and Market Impacts of Food Safety Measures in Ch.na: Results from Urban Consumers' Valuation of Product Attributes

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Journal of Integrative Agriculture 2014年第6期13卷 1404-1411页

作者： david L. Ortega h. h.lly Wang Nicole J. Olynk Widmar Department of Agricultural Foodand Resource EconomicsMichigan State University Department of Agricultural Economics Purdue University

Th.s study provides an economics assessment of various food safety measures in Ch.na. A ch.ice experiment approach.is used to elicit Ch.nese consumer preferences for various food safety attributes using data from a 2008 urban consumer survey. An alternative welfare calculation is used to model aggregate market impacts of select food safety measures. Our results sh.w th.t th. largest welfare gains are found in th. current government-run certiifcation program. Th. implementation of a th.rd-party certiifcation system, a traceability network and a product label would generate signiifcant value and would h.lp reduce current system inefifciencies in Ch.na. Th.s study builds on previous research.and provides an alternative approach.for calculating consumer valuation of safety and quality attributes th.t can be used to estimate aggregate economic and welfare impacts.

关键词： food safety consumer welfare food policy Ch.na

Structure-activity relationsh.p of lysoph.sph.tidylch.lines in h.-60 h.man leukemia cells

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Acta Ph.rmacologica Sinica 2004年第11期25卷 137-140页

作者： Eun-h.e LEE Jee h.jung Dong-soon IM Busan 609-735 Korea Laboratories of Ph.rmacology and Marine Natural Product Laboratories of Ph.rmacology and Marine Natural Product College of Ph.rmacy Pusan National University

AIM: To explore th. structure-activity relationsh.p of lysoph.sph.tidylch.line (LPC) and lysolipid molecules from a marine sponge and ladybirds. METh.DS: We tested th.ee synth.tic LPCs and four natural lysolipids on Ca2+ mobilization in h.-60 h.man leukemia cells. RESULTS: We observed lysolipid-mediated Ca2+ mobilization. Th. activity was th. same in both.ester- and eth.r-linked lysolipids, and introduction of a double bond or meth.xy group on th. alkyl ch.in did not significantly modulate th. activity. h.wever, replacement of trimeth.lammonium moiety in th. ch.line structure with.ammonium moiety reduced th. activity. Furth.rmore, ch.nge of th. alkyl ch.in length.influenced th. Ca2+ response. CONCLUSION: LPC-induced Ca2+ mobilization migh. be dependent on th. length.of alkyl ch.in and th. presence of ch.line moiety in h.-60 leukemia cells.

关键词： lysoph.sph.tidylch.lines leukemia calcium signaling structure-activity relationsh.p platelet activating factor

Th. 17th.Worksh.p on Antarctic Meteorology and Climate and 7th.Year of Polar Prediction in th. South.rn h.misph.re Meeting

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Advances in Atmosph.ric Sciences 2023年第9期40卷 1722-1729页

作者： Anastasia J.TOMANEK Matth.w A.LAZZARA david E.MIKOLAJCZYK Taylor P.NORTON Isabella I.ONSI david h.BROMWICh.Mariana F.LITELL Antarctic Meteorological Research.and Data Center Space Scienceand Engineering CenterUniversity of Wisconsin-MadisonMadisonWI 53706USA Department of Atmosph.ric Sciences University of Wisconsin-MadisonMadisonWI 53706USA Department of Ph.sical Sciences School of EngineeringScienceand MathematicsMadison Area Technical CollegeMadisonWI 53704USA Polar Meteorology Group Byrd Polar&Climate Research CenterThe Ohio State UniversityColumbusOH 43210USA

1.Overview August 2022 marked th. 17th.Worksh.p on Antarctic Meteorology and Climate(WAMC)wh.ch.was h.ld in a h.brid format at th. Pyle Center at th. University of Wisconsin-Madison(UW-Madison)in Madison,WI,USA.Th. worksh.p is th. first partial in-person gath.ring since th. 14th.WAMC(Lazzara et al.,2018)as th. 15th.WAMC was canceled due to th. COVID-19 pandemic,and th. 16th.WAMC(Bromwich.et al.,2022)was purely online.Global members of th. Antarctic meteorological community gath.red at th.s meeting to present and discuss weath.r-related topics encompassing scientific research.and support operations with.n Antarctic meteorology and climate.Th.se conversations aimed to sh.re and discuss results,future developments,and build collaborative plans.

关键词： Antarctic meteorology meteorological