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SlBEL11 regulates flavonoid biosynthesis,thus fine-tuning auxin efflux to prevent premature fruit drop in tomato

Journal of Integrative Plant Biology 2024年第4期66卷 749-770页

作者： Xiufen Dong Xianfeng Liu Lina Cheng Ruizhen Li Siqi Ge Sai Wang Yue Cai Yang Liu Sida Meng Cai-Zhong Jiang Chun-Lin Shi Tianlai Li daqi fu Mingfang Qi Tao Xu College of Horticulture Shenyang Agricultural UniversityShenyang 110866China Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province College of Horticulture ScienceZhejiang A&F UniversityHangzhou 311300China Key Laboratory for Quality and Safety Control of Subtropical Fruits and Vegetables Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang ProvinceMinistry of Agriculture and Rural AffairsCollege of Horticulture ScienceZhejiang A&F UniversityHangzhou 311300China Key Laboratory of Protected Horticulture of Ministry of Education Shenyang 110866China Crops Pathology and Genetic Research Unit United States Department of Agriculture Agricultural Research ServiceWashingtonDC 20250USA Department of Plant Sciences University of CaliforniaDavisCA 95616USA ANGENOVO Oslo 0753Norway Laboratory of Fruit Biology College of Food Science and Nutritional EngineeringChina Agricultural UniversityBeijing 100083China

Auxin regulates flower and fruit abscission,but how developmental signals mediate auxin transport in abscission remains ***,we reveal the role of the transcription factor BEL1-LIKE HOMEODOMAIN11(SlBEL11)in regulating auxin transport during abscission in tomato(Solanum lycopersicum).SlBEL11 is highly expressed in the fruit abscission zone,and its expression increases during fruit *** of SlBEL11 expression by RNA interference(RNAi)caused premature fruit drop at the breaker(Br)and 3d post-breaker(Br+3)stages of fruit *** and metabolome analysis of SlBEL11-RNAi lines revealed impaired flavonoid biosynthesis and decreased levels of most flavonoids,especially quercetin,which functions as an auxin transport *** suggested that SlBEL11 prevents premature fruit abscission by modulating auxin efflux from fruits,which is crucial for the formation of an auxin response ***,quercetin treatment suppressed premature fruit drop in SlBEL11-RNAi *** affinity purification sequencing(DAP-seq)analysis indicated that SlBEL11 induced expression of the transcription factor gene SlMYB111 by directly binding to its *** immunoprecipitation-quantitative polymerase chain reaction and electrophoretic mobility shift assay showed that *** MYELOBLASTOSIS VIRAL ONCOGENE HOMOLOG111(SlMYB111)induces the expression of the core flavonoid biosynthesis genes SlCHS1,SlCHI,SlF3H,and SlFLS by directly binding to their *** findings suggest that the SlBEL11-SlMYB111 module modulates flavonoid biosynthesis to fine-tune auxin efflux from fruits and thus maintain an auxin response gradient in the pedicel,thereby preventing premature fruit drop.

关键词： abscission auxin flavonoid SlBEL11 tomato

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冰箱负离子杀菌效果及机理

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食品科学 2024年第14期45卷 23-29页

作者：胡海梅江峰陈开松刘港帅贾雯傅达奇长虹美菱股份有限公司安徽合肥230601 中国农业大学食品科学与营养工程学院北京100083

以实验室前期在市售三文鱼、鸡胸肉、柑橘中分离纯化得到的葡萄球菌属(Staphylococcus)、假丝酵母属(Candida)和青霉属(Penicillium)菌株为目标菌,探究在冰箱4℃冷藏区负离子对这3种目标菌的杀菌效果及杀菌机理。研究结果显示,在冰箱冷... 详细信息

以实验室前期在市售三文鱼、鸡胸肉、柑橘中分离纯化得到的葡萄球菌属(Staphylococcus)、假丝酵母属(Candida)和青霉属(Penicillium)菌株为目标菌,探究在冰箱4℃冷藏区负离子对这3种目标菌的杀菌效果及杀菌机理。研究结果显示,在冰箱冷藏区中,负离子处理可有效杀灭葡萄球菌属、假丝酵母属和青霉属菌株,且杀菌效果随杀菌时间延长而增强(当杀菌时间从0.5 h延长至2 h,负离子对葡萄球菌属的杀菌效率从92.61%提高至99.29%,对假丝酵母属的杀菌效率从61.45%提高至98.26%。当杀菌时间达到1 h,可完全抑制青霉属的菌落生长)。扫描电子显微镜和透射电子显微镜观察显示,负离子处理可破坏葡萄球菌属、假丝酵母属和青霉属菌株的菌体结构,导致细胞内容物流出。负离子处理后,葡萄球菌属、假丝酵母属和青霉属菌株的菌悬液电导率及其在260 nm和280 nm处的吸光度显著上升,表明负离子处理破坏了3种目标菌体的完整性,导致细胞内容物流出,核酸和蛋白质渗漏。二乙酸荧光素染色显示,负离子处理显著降低了假丝酵母属和青霉属的细胞活力。此外,负离子处理完全抑制了青霉属孢子的萌发和芽管形成。结论:冰箱冷藏区负离子处理可通过破坏葡萄球菌属、假丝酵母属和青霉属细胞结构的完整性,降低假丝酵母属和青霉属的细胞活力,抑制青霉属孢子的萌发与芽管形成,从而有效杀灭这3种目标菌。

关键词：冰箱负离子葡萄球菌属假丝酵母属青霉属杀菌效果杀菌机理

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四种不同品种番茄果实不同组织的抗氧化活性研究

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保鲜与加工 2024年第7期24卷 102-109页

作者：胡婷婷陈笛朱鸿亮傅达奇曲桂芹中国农业大学食品科学与营养工程学院北京100083

为探究番茄果实中不同组织抗氧化活性物质的含量、活性及二者的相关性,高效利用番茄果实中的抗氧化活性物质,系统比较了2种大果番茄(‘丰收128’‘DRK0568’)、1种小果番茄(‘千禧’)、1种实验室模式番茄(‘AC’)不同组织(果皮、果肉和... 详细信息

为探究番茄果实中不同组织抗氧化活性物质的含量、活性及二者的相关性,高效利用番茄果实中的抗氧化活性物质,系统比较了2种大果番茄(‘丰收128’‘DRK0568’)、1种小果番茄(‘千禧’)、1种实验室模式番茄(‘AC’)不同组织(果皮、果肉和种子)中抗氧化活性物质的含量与抗氧化活性之间的关系。结果表明,果皮是番茄红素含量最多的组织,多酚类及类黄酮类物质在果皮和种子中含量较高,抗坏血酸在果皮和果肉中的含量较高。4个番茄品种中,‘千禧’小番茄总酚、总黄酮、抗坏血酸等抗氧化活性物质的含量均高于其他3个品种。果皮组织中亲水提取物的2,2-联氨-双-3-乙基苯并噻唑啉-6-磺酸(2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid),ABTS)阳离子自由基清除率与总黄酮含量显著相关,铁离子还原抗氧化活性与总酚含量、抗坏血酸含量均极显著相关,亲脂提取物抗氧化活性与总酚和总黄酮和番茄红素含量均极显著相关;种子中的亲水提取物和亲脂提取物抗氧化活性与总黄酮和番茄红素含量有较高的相关性;果肉中的亲水提取物抗氧化活性与抗坏血酸含量的相关性较高,亲脂提取物抗氧化活性与番茄红素含量的相关性最高。综上可知,番茄的果皮和种子含有较丰富的抗氧化活性成分,抗氧化能力相对较高。本研究结果可为高效利用番茄中的抗氧化活性物质及健康饮食提供指导。

关键词：番茄品种抗氧化活性抗氧化活性物质

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Applications of virus-induced gene silencing for identification of gene function in fruit

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Food Quality and Safety 2021年第3期5卷 222-230页

作者： Gangshuai Liu Hongli Li daqi fu Laboratory of Fruit Biology College of Food Science&Nutritional EngineeringChina Agricultural UniversityBeijingChina

With the development of bioinformatics,it is easy to obtain information and data about thousands of genes,but the determi nation of the functions of these genes depends on methods for rapid and effective functi on al ***-induced gene sile ncing(VIGS)is a mature method of gene functional identification developed over the last 20 years,which has been widely used in many research fields involving many *** quality formation is a complex biological process,which is closely related to ***,we review the progress and contribution of VIGS to our understanding of fruit biology and its advantages and disadvantages in determining gene function.

关键词： Gene function virus-induced gene silencing fruit viral vector.

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肉品鲜味物质研究进展

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食品科学 2024年第19期45卷 384-394页

作者：谢诗音苏笑胡海梅陈开松江峰傅达奇中国农业大学食品科学与营养工程学院北京100083 长虹美菱股份有限公司安徽合肥230601

鲜味作为五大基本味觉之一,是肉品重要的感官特性,亦是人们评价肉品品质的重要指标。肉品的鲜味主要来源于肉中的鲜味物质,如氨基酸、核苷酸、多肽等。鲜味物质以其有助于改善食物风味、增强人们食欲等特点,逐渐成为了食品鲜味科学领域... 详细信息

鲜味作为五大基本味觉之一,是肉品重要的感官特性,亦是人们评价肉品品质的重要指标。肉品的鲜味主要来源于肉中的鲜味物质,如氨基酸、核苷酸、多肽等。鲜味物质以其有助于改善食物风味、增强人们食欲等特点,逐渐成为了食品鲜味科学领域的研究热点。本文对肉品中鲜味物质的分类、呈味机理和影响因素以及主要鲜味物质的检测方法进行总结和归纳,以期为肉品中鲜味物质的理论研究和实际应用提供参考。

关键词：肉品鲜味物质呈味机理影响因素检测

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Effects of 1-methylcyclopropene treatment on the quality of red‘fuji’apples fruit during short-term storage

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Food Quality and Safety 2023年第1期7卷 63-74页

作者：彭贞贞傅达奇 Laboratory of Fruit Biology College of Food Science and Nutritional EngineeringChina Agricultural UniversityBeijingChina

Objectives:The aim of this study is to determine the mechanism through which 1-methylcyclopropene(1-MCP)affects the quality of red'fuji'apples,which were stored for a short *** and Methods:Red‘fuji’apples were treated with 1-MCP(1.0μl/L),stored at 25℃for0,12,24,48,72,and 96 h,and ethylene pro-duction was *** integrated metabolomic and transcriptomic analysis was performed on apples stored for 24 ***:The release of ethylene was significantly delayed from red‘fuji’apples subjected to 1-MCP *** performing an integrated transcriptome and metabolome analyses,we identified 117 differentially expressed genes(DEGs)and 44 differentially accumulated metabol-ites(DAMs).By performing functional enrichment analysis,we found that DEGs were involved in the following pathways;carbon metabolism(LPD2,gpmA,LTA2,ACC,PSAT1,MdCAS2),phytohormone signal transduction(EBF 7),amino acid metabolism(MdACS-1),fatty acid metab-olism(LOX1.5,KCS4,KAST),energy metabolism(Lhcb1,Lhcb6,PsbY,GPDHC1,PUMP5I,metabolic pathways(TRE1,HEXO1)and cell wall metabolism(CSLG2).Thus,these DEGs were involved in the ripening of fruits,and they controlled the quality of fruits at the post-harvest *** metabolites were enriched with *** were found to be individually involved in the metabolic pathway,secondary metabolite bio-synthesis,phenylpropanoid biosynthesis,flavonoids,and flavonol ***:The results indicate that 1-MCP inhibits the biosynthesis of ethylene and suppresses energy ***,it also downregulates metabolic pathways and the enzymatic genes related to fruit ***,1-MCP delays the ripening of fruits at the post-harvest *** study helps us understand how 1-MCP treatment affects the ripening and quality of fruits.

关键词： Red‘fuji’apple 1-methylcyclopropene metabolome transcriptome.

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Deciphering Ascorbic Acid Regulatory Pathways in Ripening Tomato Fruit Using a Weighted Gene Correlation Network Analysis Approach

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Journal of Integrative Plant Biology 2013年第11期55卷 1080-1091页

作者： Chao Gao Zheng Ju Shan Li Jinhua Zuo daqi fu Huiqin Tian Yunbo Luo Benzhong Zhu Laboratory of Fruit Biology College of Food Science and Nutritional Engineering China Agricultural University Laboratory of Postharvest Storage and Processing of Vegetables National Engineering Research Center for Vegetables

Genotype is generally determined by the co-expression of diverse genes and multiple regulatory pathways in plants. Gene co-expression analysis combining with physiological trait data provides very important information about the gene function and regulatory mechanism. L-Ascorbic acid （AsA）, which is an essential nutrient component for human health and plant metabolism, plays key roles in diverse biological processes such as cell cycle, cell expansion, stress resistance, hormone synthesis, and signaling. Here, we applied a weighted gene correlation network analysis approach based on gene expression values and AsA content data in ripening tomato （Solanum lycopersicum L.） fruit with different AsA content levels, which leads to identification of AsA relevant modules and vital genes in AsA regulatory pathways. Twenty- four modules were compartmentalized according to gene expression profiling. Among these modules, one negatively related module containing genes involved in redox processes and one positively related module enriched with genes involved in AsA biosynthetic and recycling pathways were further analyzed. The present work herein indicates that redox pathways as well as hormone-signal pathways are closely correlated with AsA accumulation in ripening tomato fruit, and allowed us to prioritize candidate genes for follow-up studies to dissect this interplay at the biochemical and molecular level.

关键词： Gene co-expression L-ascorbic acid network system biology weighted gene correlation network analysis approach.

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Virus-induced Gene Silencing in Eggplant(Solanum melongena)

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Journal of Integrative Plant Biology 2012年第6期54卷 422-429页

作者： Haiping Liu daqi fu Benzhong Zhu Huaxue Yan Xiaoying Shen Jinhua Zuo Yi Zhu Yunbo Luo Laboratory of Fruit Biology College of Food Science&Nutritional EngineeringChina Agricultural UniversityBeijing 100083China

Eggplant （Solanum melongena） is an economically important vegetable requiring investigation into its various genomic functions. The current limitation in the investigation of genomic function in eggplant is the lack of effective tools available for conducting functional assays. Virus-induced gene silencing （VIGS） has played a critical role in the functional genetic analyses. In this paper, TRV-mediated VIGS was successfully elicited in eggplant. We first cloned the CDS sequence of PDS （PHYTOENE DESATURASE） in eggplant and then silenced the PDS gene. Photo-bleaching was shown on the newly-developed leaves four weeks after agroinoculation, indicating that VIGS can be used to silence genes in eggplant. To further illustrate the reliability of VIGS in eggplant, we selected Chl H, Su and CLA1 as reporters to elicit VIGS using the high-pressure spray method. Suppression of Chl H and Su led to yellow leaves, while the depletion of CLA1 resulted in albino. In conclusion, four genes, PDS, Chl H, Su （Sulfur）, CLA1, were down-regulated significantly by VIGS, indicating that the VIGS system can be successfully applied in eggplant and is a reliable tool for the study of gene function.

关键词： Virus-induced gene silencing tobacco rattle virus PHYTOENE DES, ATURASE Solanum melongena

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MicroRNAs in tomato plants

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Science China(Life Sciences) 2011年第7期54卷 599-605页

作者： ZUO JinHua WANG YunXiang LIU HaiPing MA YuanZheng JU Zheng ZHAI BaiQiang fu daqi ZHU Yi LUO YunBo ZHU BenZhong College of Food Science and Nutritional Engineering China Agricultural University Beijing 100083 China

MicroRNAs (miRNAs) are a specialized class of small silencing RNAs that regulate gene expression in *** plants,miRNAs negatively regulate target mRNAs containing a highly complementary sequence by either mRNA cleavage or translational *** a model plant to study fleshy fruit ripening,miRNA studies in tomato have made great progress *** were predicted to be involved in nearly all biological processes in tomato,particularly development,differentiation,and biotic and abiotic stress ***,several miRNAs were verified to be involved in tomato fruit ripening and *** studies suggest that miRNAs are related to host-virus interactions,which raises the possibility that miRNAs can be used as diagnostic markers for response to virus infection in tomato *** this review,we summarize our current knowledge systematically and advance future directions for miRNA research in tomato.

关键词： microRNAs tomato research methods target gene prediction functional analysis

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不同贮藏温度对京彩1号西瓜品质的影响

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中国农业科技导报 2024年第9期26卷 137-145页

作者：向兰婷宋曙辉刘立娟佘小玲周家华王宝刚常虹张超傅达奇王云香北京市农林科学院农产品加工与食品营养研究所果蔬农产品保鲜与加工北京市重点实验室农业农村部蔬菜产后处理重点实验室北京100097 中国农业大学食品科学与营养工程学院北京100083 北京市延庆区农业科技服务中心北京102100

为探究不同贮藏温度对京彩1号西瓜品质的影响,将京彩1号西瓜果实分别在5、15、25℃温度下贮藏,研究贮藏期间其硬度、失重率、冷害率、可溶性固形物、果胶、纤维素、可溶性糖、有机酸、番茄红素、β-胡萝卜素和维生素C含量的变化。结果表... 详细信息

为探究不同贮藏温度对京彩1号西瓜品质的影响,将京彩1号西瓜果实分别在5、15、25℃温度下贮藏,研究贮藏期间其硬度、失重率、冷害率、可溶性固形物、果胶、纤维素、可溶性糖、有机酸、番茄红素、β-胡萝卜素和维生素C含量的变化。结果表明,随着贮藏时间的延长,京彩1号西瓜果实失重率增加,硬度降低,水溶性果胶含量升高,碱溶性果胶含量降低,维生素C、番茄红素及β-胡萝卜素含量均呈降低趋势,可溶性固形物含量变化较小,纤维素含量呈先降低后升高趋势,而可溶性糖和有机酸含量在不同贮藏温度下呈现不同变化趋势。对不同温度处理比较发现,5℃条件下贮藏可以使西瓜的维生素C及可溶性糖含量保持较高水平,但长期贮藏冷害率较高,且营养物质逐渐损失,可能会导致其失去食用价值和商品价值;15℃条件下贮藏可以较好地维持西瓜果实的水分、有机酸和细胞色素含量,使其具有较好的品质和风味;25℃条件下贮藏,西瓜果实的可溶性糖、有机酸及果胶含量消耗过快,进而造成软腐,丧失原有风味,品质不易保持。以上研究结果为不同条件下西瓜商业化物流贮运提供了参考。

关键词：西瓜贮藏温度品质

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