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Fusarivirus accessory helicases present an evolutionary link for viruses infecting plants and fungi

Virologica Sinica 2022年第3期37卷 427-436页

作者： Assane Hamidou Abdoulaye Jichun Jia Aqleem Abbas Du Hai Jiasen Cheng Yanping Fu Yang Lin daohong jiang Jiatao Xie State Key Laboratory of Agricultural Microbiology Huazhong Agricultural UniversityWuhan430070China Hubei Key Laboratory of Plant Pathology College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhan430070China Hubei Hongshan Laboratory Wuhan430070China

A significant number of mycoviruses have been identified that are related to plant viruses,but their evolutionary relationships are largely unexplored.A fusarivirus,Rhizoctonia solani fusarivirus 4(RsFV4),was identified in phytopathogenic fungus Rhizoctonia solani(R.solani)strain XY74 co-infected by an alphaendornavirus.RsFV4 had a genome of 10,833 nt(excluding the poly-A tail),and consisted of four non-overlapping open reading frames(ORFs).ORF1 encodes an 825 aa protein containing a conserved helicase domain(Hel1).ORF3 encodes 1550 aa protein with two conserved domains,namely an RNA-dependent RNA polymerase(RdRp)and another helicase(Hel2).The ORF2 and ORF4 likely encode two hypothetical proteins(520 and 542 aa)with unknown functions.The phylogenetic analysis based on Hel2 and RdRp suggest that RsFV4 was positioned within the fusarivirus group,but formed an independent branch with three previously reported fusariviruses of R.solani.Notably,the Hel1 and its relatives were phylogenetically closer to helicases of potyviruses and hypoviruses than fusariviruses,suggesting fusarivirus Hel1 formed an evolutionary link between these three virus groups.This finding provides evidence of the occurrence of a horizontal gene transfer or recombination event between mycoviruses and plant viruses or between mycoviruses.Our findings are likely to enhance the understanding of virus evolution and diversity.

关键词： Fusarivirus Potyvirus Hypovirus Helicase Horizontal gene transfer(HGT)

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Active DNA demethylation regulates MAMP-triggered immune priming in Arabidopsis

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Journal of Genetics and Genomics 2022年第8期49卷 796-809页

作者： Mengling Huang Ying Zhang Ying Wang Jiatao Xie Jiasen Cheng Yanping Fu daohong jiang Xiao Yu Bo Li State Key Laboratory of Agricultural Microbiology Huazhong Agricultural UniversityWuhanHubei 430070China The Provincial Key Lab of Plant Pathology of Hubei Province College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanHubei 430070China Hubei Hongshan Laboratory WuhanHubei 430070China

Plants recognize microbe-associated molecular patterns(MAMPs)to activate immune responses and defense priming to defend against pathogen infections.Transcriptional regulation of gene expression is crucial for plant immunity and is mediated by multiple factors,including DNA methylation.However,it remains unknown whether and how DNA demethylation contributes to immune responses in MAMPtriggered immunity.Here,we report that active DNA demethylation is required for MAMP-triggered immunity to bacterial pathogens.The rdd-2 triple mutant carrying mutations in ROS1,DML2,and DML3 that encode DNA glycosylases,which are key DNA demethylation enzymes,exhibits compromised immune responses triggered by the MAMPs fig22 and elf18.Genome-wide methylome analysis reveals that fig22 induces rapid and specific DNA demethylation in an RDD-dependent manner.The expression levels of salicylic acid signaling-related and phytoalexin biosynthesis-related genes are tightly associated with the fig22-induced promoter demethylation.The compromised accumulation of priming compounds and antimicrobial metabolites ultimately leads to a defense priming defect in the rdd-2 mutant.Our results reveal the critical role of active DNA demethylation in the MAMP-triggered immune response and provide unique insight into the molecular mechanism of fig22-modulated DNA demethylation.

关键词： Pattern-triggered immunity Immune priming Defense gene expression DNA demethylation Immune signal transduction

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Enrichment of bacteria involved in the nitrogen cycle and plant growth promotion in soil by sclerotia of rice sheath blight fungus

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Stress Biology 2022年第1期2卷 299-311页

作者： Mirza Abid Mehmood Yanping Fu Huizhang Zhao Jiasen Cheng Jiatao Xie daohong jiang State Key Laboratory of Agricultural Microbiology Huazhong Agricultural UniversityHubei ProvinceWuhan 430070China Plant Pathology Institute of Plant ProtectionMNS University of AgricultureMultan 60000Pakistan Provincial Key Laboratory of Plant Pathology of Hubei Province College of Plant Science and TechnologyHuazhong Agricultural UniversityHubei ProvinceWuhan 430070China Hubei Hongshan Laboratory Wuhan 430070China

Rice sheath blight pathogen,Rhizoctonia solani,produces numerous sclerotia to overwinter.As a rich source of nutrients in the soil,sclerotia may lead to the change of soil microbiota.For this purpose,we amended the sclerotia of R.solani in soil and analyzed the changes in bacterial microbiota within the soil at different time points.At the phyla level,Proteobacteria,Acidobacteria,Bacteroidetes,Actinobacteria,Chloroflexi and Firmicutes showed varied abundance in the amended soil samples compared to those in the control.An increased abundance of ammonia-oxidizing bacterium(AOB)Nitrosospira and Nitrite oxidizing bacteria(NOB)i.e.,Nitrospira was observed,where the latter is reportedly involved in the nitrifier denitrification.Moreover,Thiobacillus,Gemmatimonas,Anaeromyxobacter and Geobacter,the vital players in denitrification,N2O reduction and reductive nitrogen transformation,respectively,depicted enhanced abundance in R.solani sclerotia-amended samples.Furthermore,asymbiotic nitrogen-fixing bacteria,notably,Azotobacter as well as Microvirga and Phenylobacterium with nitrogen-fixing potential also enriched in the amended samples compared to the control.Plant growth promoting bacteria,such as Kribbella,Chitinophaga and Flavisolibacter also enriched in the sclerotia-amended soil.As per our knowledge,this study is of its kind where pathogenic fungal sclerotia activated microbes with a potential role in N transformation and provided clues about the ecological functions of R.solani sclerotia on the stimulation of bacterial genera involved in different processes of N-cycle within the soil in the absence of host plants.

关键词： Rice sheath blight Rhizoctonia solani Sclerotia Reductive nitrogen transformation Nitrogen fixation Soil microbiome

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Transmissible hypovirulent element in isolate Ep-1PN of Sclerotinia sclerotiorum

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Chinese Science Bulletin 1998年第9期43卷 779-781页

作者： daohong jiang Guoqing Li Yanping Fu Xianhong Yi Daoben Wang Huazhong Agr Univ Dept Plant Protect Wuhan 430070 Peoples R China

The cause of virulence decline of hypovirulent isolate Ep-1PN of Sclerotinia sclerotiorum (Lib.) de Bary has been studied through transmitting test and analysing its offsprings derived from single ascospore. When the colony of a normal strain Ep-1PNA 183 intermingled with the colony of Ep-1PN, its margin became abnormal, just like Ep-1PN. Cultures isolated from top-tip of the colony of Ep-1PNA 183 showed the hypovirulence and its associated properties of Ep-1PN. Moreover, a series of cultures which have characteristics between Ep-1PN and normal strain could be isolated from stipes of Ep-1PN. More than 200 offsprings derived from single ascospore of Ep-1PN were normal strains. According to those results, there exists a transmissible cytoplasmic hypovirulent element in hypovirulent isolate Ep-1PN of S. sclerotiorum.

关键词： Sclerotinia sclerotiorum hypovirulent strain hypovirulent element Ep-1PN.

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核盘菌自噬相关基因SsATG5和SsATG8的功能分析

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植物保护学报 2024年第2期51卷 302-313页

作者：刘勇张宇宇付艳苹谢甲涛姜道宏程家森华中农业大学农业微生物学国家重点实验室武汉430070 华中农业大学植物科学技术学院湖北省作物病害监测和安全控制重点实验室武汉430070

为探究自噬在核盘菌Sclerotinia sclerotiorum致病过程中的作用,利用酵母Saccharomyces自噬相关基因(autophagy-related gene,ATG)编码的蛋白序列比对核盘菌基因组,获得核盘菌假定ATG,并以核盘菌1980菌株为出发菌株,基于同源重组的原理... 详细信息

为探究自噬在核盘菌Sclerotinia sclerotiorum致病过程中的作用,利用酵母Saccharomyces自噬相关基因(autophagy-related gene,ATG)编码的蛋白序列比对核盘菌基因组,获得核盘菌假定ATG,并以核盘菌1980菌株为出发菌株,基于同源重组的原理对假定ATG进行敲除和回补,并测定不同突变体的生长表型和致病能力。结果表明,从核盘菌基因组中比对到2个ATG,分别命名为SsATG5和SsATG8,两者在核盘菌致病过程中均上调表达。SsATG5和SsATG8敲除突变体在菌丝生长、产草酸和侵染垫形成方面与野生型菌株无明显差异,但SsATG5敲除突变体在离体拟南芥Arabidopsis thaliana叶片上的致病力显著下降了约40%,在活体拟南芥植株上的致病力显著下降了约80%,同时SsATG5回补突变体恢复了正常的致病力。表明SsATG5参与了核盘菌的致病过程,证实自噬在核盘菌致病过程中发挥着重要作用。

关键词：核盘菌 SsATG5 SsATG8 自噬致病力侵染垫

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A 2-kb Mycovirus Converts a Pathogenic Fungus into a Beneficial Endophyte for Brassica Protection and Yield Enhancement

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Molecular Plant 2020年第10期13卷 1420-1433页

作者： Hongxiang Zhang Jiatao Xie Yanping Fu Jiasen Cheng Zheng Qu Zhenzhen Zhao Shufen Cheng Tao Chen Bo Li Qianqian Wang Xinqiang Liu Binnian Tian David B.Collinge daohong jiang State Key Laboratory of Agricultural Microbiology College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanHubei Province 430070China The Provincial Key Lab of Plant Pathology of Hubei Province Huazhong Agricultural UniversityWuhanChina Department of Plant and Environmental Sciences University of Copenhagen1871 Frederiksberg CDenmark

Mycoviruses are viruses that infect fungi,and hypovirulence-associated mycoviruses have the potential to control fungal diseases.However,it is unclear how mycovirus-mediated hypovirulent strains live and survive in the field,and no mycovirus has been applied for field crop protection.In this study,we found that a previously identified small DNA mycovirus(SsHADV-1)can convert its host,Sclerotinia sclerotiorum,from a typical necrotrophic pathogen to a beneficial endophytic fungus.SsHADV-1 downregulates the expression of key pathogenicity factor genes in S.sclerotiorum during infection.When growing in rapeseed,the SsHADV-1-infected strain DT-8 significantly regulates the expression of rapeseed genes involved in defense,hormone signaling,and circadian rhythm pathways.As a result,plant growth is promoted and disease resistance is enhanced.Field experiments showed that spraying DT-8 at the early flowering stage can reduce the disease severity of rapeseed stem rot by 67.6%and improve yield by 14.9%.Moreover,we discovered that SsHADV-1 could also infect other S.sclerotiorum strains on DT-8-inoculated plants and that DT-8 could be recovered from dead plants.These findings suggest that the mycoviruses may have the ability to shape the origin of endophytism.Our discoveries suggest that mycoviruses may influence the origin of endophytism and may also offer a novel strategy for disease control in which mycovirus-infected strains are used to improve crop health and release mycoviruses into the field.

关键词： mycovirus Sclerotinia sclerotiorum endophyte rapeseed stem rot biological control Brassica napus

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植物内生真菌及其展望

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中国生物防治学报 2015年第5期31卷 742-749页

作者：姜道宏华中农业大学植物科学技术学院/农业微生物学国家重点实验室武汉430070

内生真菌在自然界广泛存在,有些内生真菌可以促进植物营养吸收、提高植物抗生物逆境和非生物逆境的能力、刺激植物产生活性物质等。因此,内生真菌是植物病虫害生物防治领域的重要研究内容之一。本文就内生真菌与寄主共生的分子基础、内... 详细信息

内生真菌在自然界广泛存在,有些内生真菌可以促进植物营养吸收、提高植物抗生物逆境和非生物逆境的能力、刺激植物产生活性物质等。因此,内生真菌是植物病虫害生物防治领域的重要研究内容之一。本文就内生真菌与寄主共生的分子基础、内生真菌对植物影响机制、内生真菌与寄主特异性相互作用、特殊的内生真菌——植物病原真菌、内生真菌形成的分子机制和内生真菌研究展望等6个方面对内生真菌进行了叙述,期望为科学地利用内生真菌资源提供一些参考。

关键词：内生真菌植物病原真菌互惠共生共生功能体生物防治微生物组

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NADPH氧化酶相关基因对盾壳霉代谢的影响

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华中农业大学学报 2019年第5期38卷 79-84页

作者：孙池蒋静胡先文付艳苹姜道宏华中农业大学理学院武汉430070 华中农业大学植物科学技术学院武汉430070

基于液质联用(LC-MS)的代谢组学分析手段,通过突变株Qnox1-6与野生株zs-1的代谢比较,研究NADPH氧化酶相关基因对盾壳霉代谢的影响。筛选得到14个重要的代谢差异物质,主要是羧酸类和苷类物质。特别是7个仅在突变株中出现的物质为:苯二酚... 详细信息

基于液质联用(LC-MS)的代谢组学分析手段,通过突变株Qnox1-6与野生株zs-1的代谢比较,研究NADPH氧化酶相关基因对盾壳霉代谢的影响。筛选得到14个重要的代谢差异物质,主要是羧酸类和苷类物质。特别是7个仅在突变株中出现的物质为:苯二酚、松柏苷、甾体皂苷类物质、3个羧酸类物质及其他物质,这些代谢物与盾壳霉寄生核盘菌紧密相关。

关键词：盾壳霉 NOX 突变株寄生能力 LC-MS 差异代谢物代谢组学

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军队应尽快实现“三个转变”

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国防科技 2017年第4期38卷 1-4页

作者：姜道洪沈阳军区善后办辽宁沈阳110805

现代科学技术的广泛军事应用,一方面引起了作战方式方法和手段的深刻变化,另一方面则对军人素质和军事教育训练提出了新的更高要求。我们必须着眼习主席提出的"能打仗、打胜仗"强军目标,尽快实现"三个转变",即士兵要由"娇弱型"向"强悍型... 详细信息

现代科学技术的广泛军事应用,一方面引起了作战方式方法和手段的深刻变化,另一方面则对军人素质和军事教育训练提出了新的更高要求。我们必须着眼习主席提出的"能打仗、打胜仗"强军目标,尽快实现"三个转变",即士兵要由"娇弱型"向"强悍型"转变,军官要由"管理型"向"指挥型"转变,部队要由"生活型"向"打仗型"转变。

关键词：科学技术强军目标三个转变

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盾壳霉对2种除草剂的敏感性评价

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华中农业大学学报 2018年第5期37卷 25-31页

作者：强翠翠程家森谢甲涛付艳苹姜道宏华中农业大学农业微生物学国家重点实验室武汉430070 华中农业大学湖北省作物病害监测和安全控制重点实验室武汉430070

为了与播前除草措施相配合,达到油菜轻简化栽培的目的,开展了盾壳霉对田间常用灭生型除草剂草甘膦和草铵膦敏感性的研究。结果发现,草甘膦和草铵膦原药对盾壳霉菌丝生长有较强抑制作用,EC50分别为714.3μg/mL和574.5μg/mL;草甘膦对盾... 详细信息

为了与播前除草措施相配合,达到油菜轻简化栽培的目的,开展了盾壳霉对田间常用灭生型除草剂草甘膦和草铵膦敏感性的研究。结果发现,草甘膦和草铵膦原药对盾壳霉菌丝生长有较强抑制作用,EC50分别为714.3μg/mL和574.5μg/mL;草甘膦对盾壳霉孢子萌发抑制作用较弱,EC50为1 019.8μg/mL,而草铵膦原药对孢子的萌发基本没有抑制作用;2种除草剂原药均抑制核盘菌菌丝生长,EC50分别为1 695.8μg/mL和223.5μg/mL;2种除草剂商品制剂对盾壳霉孢子没有杀灭作用,但延迟孢子的萌发,影响盾壳霉对核盘菌菌核的寄生。研究结果表明,在田间生产中盾壳霉可以与草甘膦和草铵膦2种除草剂进行混合施用。

关键词：盾壳霉核盘菌草甘膦草铵膦敏感性

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