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Knockout of butyrophilin subfamily 1 member A1(BTN1A1) alters lipid droplet formation and phospholipid composition in bovine mammary epithelial cells

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Journal of Animal Science and Biotechnology 2020年第4期11卷 975-985页

作者： Liqiang Han Menglu Zhang Zhiyang Xing danielle N.Coleman Yusheng Liang Juan J.Loor Guoyu Yang College of Animal Science and Veterinary Medicine Henan Agricultural UniversityZhengzhou 450002PR China Department of Animal Sciences and Division of Nutritional Sciences University of IllinoisUrbanaIllinois 61801USA.

Background: Milk lipids originate from cytoplasmic lipid droplets(LD) that are synthesized and secreted from mammary epithelial cells by a unique membrane-envelopment process. Butyrophilin 1 A1(BTN1 A1) is one of the membrane proteins that surrounds LD, but its role in bovine mammary lipid droplet synthesis and secretion is not well ***: The objective was to knockout BTN1 A1 in bovine mammary epithelial cells(BMEC) via the CRISPR/Cas9 system and evaluate LD formation, abundance of lipogenic enzymes, and content of cell membrane phospholipid(PL) species. Average LD diameter was determined via Oil Red O staining, and profiling of cell membrane phospholipid species via liquid chromatography-tandem mass spectrometry(LC-MS/MS).Results: Lentivirus-mediated infection of the Cas9/sg RNA expression vector into BMEC resulted in production of a homozygous clone BTN1 A1^((-/-)). The LD size and content decreased following BTN1 A1 gene knockout. The m RNA abundance of fatty acid synthase(FASN) and peroxisome proliferator-activated receptor-gamma(PPARG) was downregulated in the BTN1 A1^((-/-))clone. Subcellular analyses indicated that BTN1 A1 and LD were co-localized in the cytoplasm. BTN1 A1 gene knockout increased the percentage of phosphatidylethanolamine(PE) and decreased phosphatidylcholine(PC), which resulted in a lower PC/PE ***: Results suggest that BTN1 A1 plays an important role in regulating LD synthesis via a mechanism involving membrane phospholipid composition.

关键词： Lipid droplet Mammary epithelial cell Milk fat globule Phospholipid

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Preoperative virtual video visits only:a convenient option that should be offered to caregivers beyond the pandemic

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World Journal of Pediatric Surgery 2022年第3期5卷 169-175页

作者： danielle Dougherty Allison R Thompson Karen Elizabeth Speck Erin E Perrone Section of Pediatric Surgery Department of SurgeryUniversity of Michigan Health SystemAnn ArborMichiganUSA Department of Health Behavior and Health Education University of Michigan School of Public HealthAnn ArborMIUSA

Objective During the COVID-19 pandemic,our group implemented preoperative video visits(VVs)to limit physical *** aim of this study was to determine caregivers’and providers’perceptions of this practice and to determine feasibility for *** All patients who had only a preoperative VV prior to an elective surgery were identified from March-October ***,surgeons,and clinic staff were surveyed about their *** Thirty-four preoperative VVs were followed by an elective surgery without a preceding in-person *** the 31 caregiver surveys completed,the majority strongly agreed that the VV was more convenient(87%,n=27).Eighty-one percent(n=25)strongly agreed or agreed that the VV saved them ***-four percent(n=29)strongly agreed or agreed that they would choose the VV option *** saved an average travel distance of 60.3 miles one way(range 6.1-480).Of the 13/17 providers who responded,77%(n=10)expressed that the practice should *** Virtual health became a necessity during the pandemic,and caregivers were overwhelmingly *** VVs as an option beyond the pandemic may be a reasonable and effective way to help eliminate some of the hurdles that impede healthcare-seeking behavior and should be offered.

关键词： convenient beyond eliminate

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Antimicrobial peptides and the gut microbiome in inflammatory bowel disease

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World Journal of Gastroenterology 2021年第43期27卷 7402-7422页

作者： John Gubatan Derek R Holman Christopher J Puntasecca danielle Polevoi Samuel JS Rubin Stephan Rogalla Division of Gastroenterology and Hepatology Stanford University School of MedicineRedwood CityCA 94063United States Department of Radiology Molecular Imaging Program at StanfordStanford UniversityStanfordCA 94305United States Stanford University School of Medicine Stanford UniversityStanfordCA 94063United States

Antimicrobial peptides(AMP)are highly diverse and dynamic molecules that are expressed by specific intestinal epithelial cells,Paneth cells,as well as immune cells in the gastrointestinal(GI)*** play critical roles in maintaining tolerance to gut microbiota and protecting against enteric *** that disruptions in tolerance to commensal microbiota and loss of barrier function play major roles in the pathogenesis of inflammatory bowel disease(IBD)and converge on the function of AMP,the significance of AMP as potential biomarkers and novel therapeutic targets in IBD have been increasingly recognized in recent *** this frontier article,we discuss the function and mechanisms of AMP in the GI tract,examine the interaction of AMP with the gut microbiome,explore the role of AMP in the pathogenesis of IBD,and review translational applications of AMP in patients with IBD.

关键词： Antimicrobial peptides Inflammatory bowel disease Ulcerative colitis Crohn’s disease Gut microbiome Biomarkers

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Juvenile polyposis syndrome

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World Journal of Gastroenterology 2011年第44期17卷 4839-4844页

作者： Lodewijk AA Brosens danielle Langeveld W Arnout van Hattem Francis M Giardiello G Johan A Offerhaus Department of Pathology University Medical Centre Utrecht Department of Pathology Academic Medical Centre Division of Gastroenterology Department of Medicine The Johns Hopkins University School of Medicine

Juvenile polyposis syndrome is a rare autosomal dominant syndrome characterized by multiple distinct juvenile polyps in the gastrointestinal tract and an increased risk of colorectal *** cumulative life-time risk of colorectal cancer is 39% and the relative risk is *** polyps have a distinctive histology characterized by an abundance of edematous lamina propria with inflammatory cells and cystically dilated glands lined by cuboidal to columnar epithelium with reactive ***,juvenile polyposis syndrome is defined by the presence of 5 or more juvenile polyps in the colorectum,juvenile polyps throughout the gastrointestinal tract or any number of juvenile polyps and a positive family history of juvenile *** about 50%-60% of patients diagnosed with juvenile polyposis syndrome a germline mutation in the SMAD4 or BMPR1A gene is *** genes play a role in the BMP/TGF-beta signalling *** has been suggested that cancer in juvenile polyposis may develop through the socalled "landscaper mechanism" where an abnormal stromal environment leads to neoplastic transformation of the adjacent epithelium and in the end invasive *** of this rare disorder is important for patients and their families with regard to treatment,follow-up and screening of at risk *** clinician confronted with the diagnosis of a juvenile polyp should therefore consider the possibility of juvenile polyposis *** addition,juvenile polyposis syndrome provides a unique model to study colorectal cancer pathogenesis in general and gives insight in the molecular genetic basis of *** review discusses clinical manifestations,genetics,pathogenesis and management of juvenile polyposis syndrome.

关键词： Juvenile polyposis syndrome Hamartoma Colorectal cancer SMAD4 BMPR1A

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Adsorption of methylene blue onto activated carbon produced from tea (Camellia sinensis L.) seed shells: kinetics, equilibrium, and thermodynamics studies

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第7期14卷 650-658页

作者： Jun-jie GAO Ye-bo QIN Tao ZHOU Dong-dong CAO Ping XU danielle HOCHSTETTER Yue-fei WANG Department of Tea Science Zhejiang University Crop Management Bureau Agricultural Department of Zhejiang Province Zhejiang Academy of Agricultural Sciences Key Laboratory of Horticultural Plant Growth Development and Quality Improvement Ministry of Agriculture

Tea (Camellia sinensis L.) seed shells, the main byproduct of the manufacture of tea seed oil, were used as precursors for the preparation of tea activated carbon (TAC) in the present study. A high yield (44.1%) of TAC was obtained from tea seed shells via a one-step chemical method using ZnCl2 as an agent. The Brunauer-Emmett-Teller (BET) surface area and the total pore volumes of the obtained TAC were found to be 1 530.67 mg2/g and 0.7826 cm3/g, respectively. The equilibrium adsorption results were complied with Langmuir isotherm model and its maximum monolayer adsorption capacity was 324.7 mg/g for methylene blue. Adsorption kinetics studies indicated that the pseudosecond-order model yielded the best fit for the kinetic data. An intraparticle diffusion model suggested that the intraparticle diffusion was not the only rate-controlling step. Thermodynamics studies revealed the spontaneous and exothermic nature of the sorption process. These results indicate that tea seed shells could be utilized as a renewable resource to develop activated carbon which is a potential adsorbent for methylene blue.

关键词： Activated carbon Adsorption Tea seed shells Methylene blue

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Iron Speciation of Mud Breccia from the Dushanzi Mud Volcano in the Xinjiang Uygur Autonomous Region,NW China

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Acta Geologica Sinica(English Edition) 2018年第6期92卷 2201-2213页

作者： XU Wang ZHENG Guodong MA Xiangxian danielle FORTIN David R.HILTON LIANG Shouyun CHEN Zhi HU Guoyi Key Laboratory of Petroleum Resources Gansu Province/Key Laboratory of Petroleum Resources ResearchInstitute of Geology and GeophysicsChinese Academy of SciencesLanzhou 730000China University of Chinese Academy of Sciences Beijing 100049China Department of Earth and Environmental Sciences University of Ottawa25 Templeton St.OttawaOntarioK1N 6N5Canada Scripps Institution of Oceanography University of California San DiegoLa JollaCA 92093-0244USA Key Laboratory of Mechanics on Disaster and Environment in Western China(Lanzhou University) Ministry of EducationLanzhou 730000China CEA Key Laboratory of Earthquake Prediction(Institute of Earthquake Forecasting) China Earthquake AdministrationBeijing 100036China Research Institute of Petroleum Exploration and Development PetroChinaBeijing 100083China

Organic-inorganic interactions occurring in petroleum-related mud volcanoes can help predict the chemical processes that are responsible for methane emissions to the atmosphere. Seven samples of mud breccia directly ejected from one crater were collected in the Dushanzi mud volcano, along with one argillite sample of the original reddish host rocks distal from the crater, for comparison purposes. The mineral and chemical compositions as well as iron species of all samples were determined using XRD, XRF and M?ssbauer spectroscopy, respectively. The results indicate that a series of marked reactions occurred in the mud volcano systems, more specifically in the mud breccia when compared to the original rocks. Changes mainly included:(1) some conversion of clay minerals from smectite into chlorite and illite, and the precipitation of secondary carbonate minerals such as calcite and siderite;(2) silicon depletion and significant elemental enrichment of iron, manganese, magnesium, calcium and phosphorus; and(3) transformation of iron from ferric species in hematite and smectite into ferrous species in siderite, chlorite and illite. These geochemical reactions likely induced the color changes of the original reddish Neogene argillite to the gray or black mud breccia, as a result of reduction of elements and/or alteration of minerals associated with the oxidation of hydrocarbons. Our results also suggest that greenhouse gases emitted from the mud volcanoes are lowered through a series of methane oxidation reactions and carbon fixation(i.e., through carbonate precipitation).

关键词： mud volcano mud breccias iron species organic-inorganic interactions greenhouse gas

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Antibody response and therapy in COVID-19 patients: what can be learned for vaccine development?

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Science China(Life Sciences) 2020年第12期63卷 1833-1849页

作者： Ligong Lu Hui Zhang Meixiao Zhan Jun Jiang Hua Yin danielle JDauphars Shi-You Li Yong Li You-Wen He Zhuhai Interventional Medical Center Zhuhai Precision Medical CenterZhuhai People's HospitalZhuhai Hospital Affiliated with Jinan UniversityZhuhai 519000China First Affiliated Hospital China Medical UniversityShenyang 110001China tricision Biotherapeutic Inc. Zhuhai 519041China Department of Immunology Duke University Medical University Medical CenterDurhamNC 27710USA

The newly emerged severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) has infected millions of people and caused tremendous morbidity and mortality worldwide. Effective treatment for coronavirus disease 2019(COVID-19) due to SARSCoV-2 infection is lacking, and different therapeutic strategies are under testing. Host humoral and cellular immunity to SARSCoV-2 infection is a critical determinant for patients’ outcomes. SARS-CoV-2 infection results in seroconversion and production of anti-SARS-CoV-2 antibodies. The antibodies may suppress viral replication through neutralization but might also participate in COVID-19 pathogenesis through a process termed antibody-dependent enhancement. Rapid progress has been made in the research of antibody response and therapy in COVID-19 patients, including characterization of the clinical features of antibody responses in different populations infected by SARS-CoV-2, treatment of COVID-19 patients with convalescent plasma and intravenous immunoglobin products, isolation and characterization of a large panel of monoclonal neutralizing antibodies and early clinical testing, as well as clinical results from several COVID-19 vaccine candidates. In this review, we summarize the recent progress and discuss the implications of these findings in vaccine development.

关键词： COVID-19 seroconversion neutralizing antibody spike protein SARS-CoV-2 convalescent plasma vaccine

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Soil ecosystem changes by vegetation on old-field sites over five decades in the Brazilian Atlantic forest

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Journal of Forestry Research 2022年第2期33卷 667-677页

作者： danielle Cristina Ortiz Tancredo Augusto Feitosa de Souza Tatiani Maria Pech Marie Luise Carolina Bartz Dilmar Baretta Alexandre Siminski Júlia Carina Niemeyer Federal University of Santa Catarina Ulysses Gaboardikm 3CuritibanosSC 89520-000Brazil Postgraduate Programme in Agricultural and Natural Ecosystems(PPGEAN) Federal University of Santa CatarinaUlysses Gaboardikm 3CuritibanosSC 89520-000Brazil Centre for Functional Ecology Department of Life SciencesUniversity of Coimbra3000-456 CoimbraPortugal Department of Animal Science UDESC OesteSanta Catarina State UniversityChapecóSC 89815-630Brazil

Vegetation types alter soil ecosystems by changing soil fauna community activities and soil physi-cal-chemical ***,it is unclear how tree species(natural forest,native and exotic tree plantations)promote changes in the soil ecosystem,and if these changes alter functional groups of soil fauna and ecosystem *** determine the effects of five decades of old-field veg-etation on soil ecosystems in the Brazilian Atlantic Forest,field sampling of three ecosystems(exotic tree species Pinus elliottii ***,endangered tree species Arau-caria angustifolia(Bertol.)Kuntze plantation,and a natural ecosystem)were carried out,as well using bait-lamina tests and bioassays with collembolans,earthworms and seeds of Lactuca sativa *** sampling evaluated the soil fauna community and soil physical-chemical *** bait-lamina test in situ was carried out for 14-days to deter-mine fauna feeding activity,and the bioassays evaluated the reproduction of Folsomia candida,the avoidance of Eisenia andrei,and germination of *** in the soil from each *** results are:(1)vegetation type altered the soil fauna community composition;(2)soil fauna feeding was reduced in the plantations compared to the natural eco-system;(3)a physical barrier was created by recalcitrant litter that compromised fauna community structure and seed bank germination in situ;and,(4)changes in soil physical-chemical properties promoted decomposers.

关键词： Endangered tree species Exotic tree species Forest ecosystem Soil fauna functional groups Soil invertebrates

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MYC and gastric adenocarcinoma carcinogenesis

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World Journal of Gastroenterology 2008年第39期14卷 5962-5968页

作者： danielle Queiroz Calcagno Mariana Ferreira Leal Paulo Pimentel Assumpo Marília de Arruda Cardoso Smith Rommel Rodríguez Burbano Human Cytogenetics Laboratory Biology Science InstituteFederal University of ParáBelém PA 66075-900Brazil Genetics Division Department of Morphology and GeneticsFederal University of So PauloSo Paulo SP 04023-900Brazil Joo de Barros Barreto University Hospital Federal University of ParáBelém PA 66073-000Brazil

MYC is an oncogene involved in cell cycle regulation, cell growth arrest, cell adhesion, metabolism, ribosome biogenesis, protein synthesis, and mitochondrial function. It has been described as a key element of several carcinogenesis processes in humans. Many studies have shown an association between MYC deregulation and gastric cancer. MYC deregulation is also seen in gastric preneoplastic lesions and thus it may have a role in early gastric carcinogenesis. Several studies have suggested that amplification is the main mechanism of MYC deregulation in gastric cancer. In the present review, we focus on the deregulation of the MYC oncogene in gastric adenocarcinoma carcinogenesis, including its association with Helicobacterpylori （Hpylon] and clinical applications.

关键词： MYC Gastric adenocarcinoma Gastric preneoplastic lesions Gastric carcinogenesis Helicobacter pylori

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Assessment methods and services for older people with cancer in the United Kingdom

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World Journal of Clinical Oncology 2020年第3期11卷 152-161页

作者： Tania Kalsi danielle Harari Department of Ageing and Health Guys and St Thomas’NHS Foundation TrustLondon SE17EHUnited Kingdom King’s College London Capital HouseLondon SE13QDUnited Kingdom

BACKGROUND National cancer strategy calls for comprehensive assessments for older people but current practice across the United Kingdom is not well *** To identify current assessment methods and access to relevant supporting services for older people with *** A web-based survey(SurveyMonkey)targeting health professionals(oncologists,cancer surgeons,geriatricians,nurses and allied health professionals)was distributed January-April 2016 via United Kingdom nationally recognised professional *** were analysed in frequencies and *** Square was used to compare differences in responses between different *** 640 health care professionals *** 14.1%often/always involved geriatricians and 52.0%often/always involved general practitioners in *** wider assessments were used,they always/often influenced decision-making(40.5%)or at least sometimes(34.1%).But 30.5%-44.3%did not use structured assessment *** clinicians favoured clinical history *** used scoring tools and few wished to use them in the *** had urgent access to palliative care but only a minority had urgent access to other key supporting professionals(***,social workers,psychiatry).69.6%were interested in developing Geriatric Oncology services with *** There is variability in assessment methods for older people with cancer across the United Kingdom and variation in perceived access to supporting *** history taking was preferred to scoring *** closer links with geriatricians appears supported.

关键词： Geriatric assessment Elderly Older Cancer Support Services

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