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A novel supplement with yeast b-glucan, prebiotic, minerals and Silybum marianum synergistically modulates metabolic and inflammatory pathways and improves steatosis in obese mice

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Journal of Integrative Medicine 2021年第5期19卷 439-450页

作者： Victor Abou Nehmi Gilson Masahiro Murata Ruan Carlos Macêdo de Moraes Gabriely Cristina AlvesLima danielle araujo de miranda Katrin Radloff Raquel Galvao Figuerêdo Costa Joyce de Cassia Rosade Jesus Jéssica Alves de Freitas Nayara Izabel Viana Ruan Pimenta Katia Ramos Moreira Leite JoséPinhata Otoch Ana Flávia Marcal Pessoa Laboratory of Medical Investigation(LIM-26) Department of SurgeryUniversity of Sao Paulo Medical SchoolSao PauloSP 01246903Brazil Laboratory of Medical Investigation(LIM-29) Clinic Medical DepartmentUniversity of Sao Paulo Medical SchoolSao PauloSP 01246903Brazil department of Physiology Escola Paulista de Medicina/Universidade Federal de Sao PauloSP 04023062Brazil Max Rubner-Institut Federal Research Institute of Nutrition and FoodKarlsruheBW 76131Germany Cancer and Inflammation Program Center for Cancer ResearchNational Cancer InstituteNational Institutes of HealthBethesdaMD 20892United States Laboratory of Medical Investigation(LIM-55) Urology DepartmentUniversity of Sao Paulo Medical SchoolSao PauloSP 01246903Brazil D'Or Institute for Research and Education RJ 22281-100Brazil Program in Anesthesiology Surgical Sciencesand Perioperative MedicineUniversity of Sao PauloSao PauloSP 01246903Brazil Brazilian Academic Consortium for Integrative Health(CABSIN) Natural Products CommitteeSao PauloSP 05449-070Brazil

Objective: To evaluate the synergic effects of a novel oral supplement formulation, containing prebiotics,yeast b-glucans, minerals and silymarin(Silybum marianum), on lipid and glycidic metabolism, inflammatory and mitochondrial proteins of the liver, in control and high-fat diet-induced obese ***: After an acclimation period, 32 male C57 BL/6 mice were divided into the following groups:nonfat diet(NFD) vehicle, NFD supplemented, high-fat diet(HFD) vehicle and HFD supplemented. The vehicle and experimental formulation were administered orally by gavage once a day during the last four weeks of the diet(28 consecutive days). We then evaluated energy homeostasis, inflammation, and mitochondrial protein expression in these groups of ***: After four weeks of supplementation, study groups experienced reduced glycemia, dyslipidemia,fat, and hepatic fibrosis levels. Additionally, proliferator-activated receptor-α, AMP-activated protein kinase-1α, peroxisome proliferator-activated receptor γ co-activator-1α, and mitochondrial transcription factor A expression levels were augmented;however, levels of inhibitor of nuclear factor-κB kinase subunit a and p65 nuclear factor-κB expression, and oxidative markers were reduced. Notably, the cortisol/C-reactive protein ratio, a well-characterized marker of the hypothalamic–pituitary–adrenal axis immune interface status, was found to be modulated by the ***: We discovered that the novel supplement was able to modify different antioxidant, metabolic and inflammatory pathways, improving the energy homeostasis and inflammatory status, and consequently alleviated hepatic steatosis.

关键词： Supplement Silymarin Prebiotic Obese Mice Steatosis Inflammation Mitochondrial biogenesis

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Antimicrobial Activity, Structural, Crystallographic and Thermal Characteristics of Alpha-Titanium Phosphate Promoted by Silver Ions

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Materials Sciences and Applications 2024年第8期15卷 253-269页

作者： Enzo Erbisti Garcia Agnes Maria Cupertino Fernandes araujo Gerson Alberto Valencia Albitres Daniela de França da Silva Freitas danielle Mattos Mariano Carlos Magno Fialho Soares Sibele Piedade Cestari Marco Antônio Lemos Miguel Luis Claudio Mendes Institute of Macromolecules Professora Eloisa Mano Federal University of Rio de Janeiro Avenida Horacio Macedo 2030 Centro de Tecnologia Bloco J Ilha do Fundo Rio de Janeiro Brazil Institute of Microbiology Paulo de Ges Federal University of Rio de Janeiro Centro de Ciencias da Saude Bloco I Ilha do Fundo Rio de Janeiro Brazil Innovation in Polymer Engineering (PIEP) University of Minho Guimares Portugal

The recent global spread of the pandemic underscores the necessity of seeking new materials effective against microorganisms. Nanotechnology offers avenues for developing multifunctional materials. In this study, alpha-titanium phosphate (α-TiP) nanoparticles were synthesized and treated with silver salt to enhance their antimicrobial properties. The physicochemical characteristics and antimicrobial activity were evaluated. It was revealed by X-ray diffraction analysis that the structural integrity of α-TiP was influenced by ethylenediamine and silver ions. Distinct degradation profiles for each chemical modification were shown by thermogravimetric analysis. Infrared spectroscopy detected shifts and new absorption peaks in the spectra depending on the type of modification. Energy dispersive spectroscopy confirmed the disaggregation of α-TiP galleries following the addition of silver salt, which increased their effectiveness against microorganisms. Notably, only the sample treated with silver ions exhibited antimicrobial action. Antimicrobial activity was tested against the bacteria of medical importance Escherichia coli, Salmonella Enteritidis, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus, Listeria momocytogenes and the yeast Candida albicans. All microorganisms were inhibited by sample containing silver. Minor inhibition was observed against the Gram-positive bacteria L. monocytogenes and Bacillus cereus, while the greatest inhibition occurred against the fungus (yeast) C. albicans. The results revealed a potential application of the nanoparticles for control of microorganisms in public health.

关键词： Titanium Phosphate Silver Nitrate Antimicrobial Action

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delay Times and Clinical Outcomes in Acute Myocardial Infarction: Comparison of Periods before and during the COVID-19 Pandemic—Myocardial Infarction and the Pandemic

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World Journal of Cardiovascular Diseases 2024年第6期14卷 392-400页

作者： Clarice Teixeira Silva Araújo Arnon Salviato Mameri Thúlio Carrera Guarçoni Venturini Marcus Tadeu Aguilar Constantino Matos Igor Morais araujo Lopes Luiz Gustavo Ribeiro de Carvalho Murad Cleberson Duartes Ovani Guilherme Vassalo Morais Fernanda Venturini de Castro danielle Lopes Rocha Lucas Crespo de Barros Rodolfo Costa Sylvestre Luiz Fernando Machado Barbosa Roberto Ramos Barbosa Internal Medicine Universidade Federal do Esprito Santo (UFES) Vitria Brazil Hospital Santa Casa de Misericrdia de Vitria Vitria Brazil Hospital Santa Rita de Cssia Vitria Brazil Hospital Estadual Dr. Jayme Santos Neves Serra Brazil Grupo Kora Sade Vitria Brazil Hospital Estadual Infantil e Maternidade Alzir Bernardino Alves (HIMABA) Vila Velha Brazil

Introduction: At the beginning of the COVID-19 pandemic, a drop in the number of patients treated for cardiac emergencies raised concern about cardiovascular mortality in that period. An increase in care delay for patients with ST-segment elevation myocardial infarction (STEMI) may have affected clinical outcomes. Objectives: To analyze delay times and clinical outcomes of patients with STEMI undergoing primary percutaneous coronary intervention (PPCI), before and during the COVID-19 pandemic. Methods: Retrospective observational study that included patients with STEMI undergoing PPCI from december 2018 to July 2021. The COVID-19 pandemic cases were divided into two groups: pandemic I—from March to August 2020;and pandemic II—from September 2020 to July 2021. Patients were compared according to the period of hospitalization. Primary outcomes were delay times in assistance and clinical outcomes (acute kidney injury [AKI], post-procedural vascular complications and in-hospital mortality). Results: 108 patients were included, 39 (36.1%) in the pre-pandemic period, 13 (12.1%) in pandemic I and 56 (51.8%) in pandemic II. Time from onset of symptoms to arrival at the service and door-to-balloon time did not differ significantly among groups. Vascular complications were more frequent during the pandemic (I and II) than in the pre-pandemic period (2.5% pre-pandemic vs 15.4% pandemic vs 12.5% pandemic II;p = 0.03). AKI incidence was similar in all three periods. There was a non-significant increase in in-hospital mortality during the COVID-19 pandemic. Conclusion: In patients with STEMI, there was an increase in vascular complications and a trend toward increased mortality during the COVID-19 pandemic. delay times to admission and reperfusion did not differ significantly between before and during the pandemic.

关键词： Myocardial Infarction Primary Percutaneous Coronary Intervention Coronary Reperfusion Pandemic COVID-19

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Time-dependent impact of a high-fat diet on the intestinal barrier of male mice

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World Journal of Methodology 2024年第1期14卷 114-123页

作者： Carolline Santos miranda Daiana araujo Santana-Oliveira Isabela Lopes Vasques-Monteiro Nathan Soares Dantas-miranda Jade Sancha de Oliveira Glauser Flavia Maria Silva-Veiga Vanessa Souza-Mello department of Anatomy Rio de Janeiro State UniversityRio de Janeiro 20551030Brazil

BACKGROUND Excessive saturated fat intake compromises the integrity of the intestinal mucosa,leading to low-grade inflammation,impaired mucosal integrity,and increased intestinal permeability,resulting in the migration of lipopolysaccharide(LPS)to other *** To evaluate the chronic effects(at 10 and 16 wk)of a high-fat diet(HFD)(with 50%energy as fat)on the phylogenetic gut microbiota distribution and intestinal barrier structure and protection in C57BL/6 *** Forty adult male mice were divided into four nutritional groups,where the letters refer to the type of diet(control and HFD or HF)and the numbers refer to the period(in weeks)of diet administration:Control diet for 10 wk,HFD for 10 wk,control diet for 16 wk,and HFD for 16 *** sacrifice,biochemical,molecular,and stereological analyses were *** The HF groups were overweight,had gut dysbiosis,had a progressive decrease in occludin immunostaining,and had increased LPS *** progression reduced the number of goblet cells per large intestine area and Mucin2 expression in the HF16 group,consistent with a completely disarranged intestinal ultrastructure after 16 wk of HFD *** Chronic HFD intake causes overweight,gut dysbiosis,and morphological and functional alterations of the intestinal barrier after 10 or 16 ***-dependent reductions in goblet cell numerical density and mucus production have emerged as targets for countering obesity-driven intestinal damage.

关键词： High-fat diet Intestine Ultrastructure Goblet cells Gut microbiota

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Dynamics in the emergence of dormant and non-dormant herbaceous species from the soil seed bank from a Brazilian dry forest

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Journal of Plant Ecology 2020年第3期13卷 256-265页

作者： José Djalma de Souza Bruno Ayron de Souza Aguiar danielle Melo dos Santos Vanessa Kelly Rodrigues de araujo Júlia Arruda Simões Juliana Ramos de Andrade Elcida de Lima Araújo departamento de Biologia Universidade Federal Rural de PernambucoRecifePernambucoBrazil2Universidade Federal de Pernambuco-Centro Acadêmico de VitóriaPernambucoBrazil

Aims In dry tropical forests,herbaceous species may have dormancy mechanisms and form persistent and transient seed banks in the *** acquired,these mechanisms are efficient for the establishment and survival of these herbs,especially in forests with unpredictable climates,such as the ***,our objective was to verify whether the studied herbaceous species adopt the physical dormancy mechanism and how these natural barriers are overcome,to understand the temporal dynamics existing in the soil seed bank from a Brazilian dry tropical *** Seeds of five native herbaceous species from the Caatinga forests were selected and submitted to pre-germinative treatments for verifying the presence of physical *** collected soil samples in the rainy and dry seasons for four consecutive years and monitored the emergence of the selected herbaceous in the *** verified the differences in germination and seed bank emergence in the soil by generalized linear *** Findings The presence and absence of physical dormancy were observed in seeds from Caatinga herbaceous *** found intraspecific and interspecific differences in the herbaceous emergence from soil seed banks between years and climatic *** perennial herbs,consecutive lack of emergence between seasons and years was frequent,which suggests a direct relationship with the mechanism of physical dormancy and the environmental conditions necessary to overcome integument *** these species,seed dimorphism and dormancy may confer additional advantages to their ***,presenting intermediate levels of physical dormancy in an annual species may be an evolutionary adjustment to rainfall *** contrast,we found that the annual herb without dormancy is more sensitive to seasonal and interannual climate changes,as evidenced by the increase and significant reduction of its emergence in the soil seed *** differences acquired evoluti

关键词： establishment herbaceous species physical dormancy seed bank seed germination semiarid

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Fraud investigation in commercial coffee by chromatography

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Food Quality and Safety 2018年第3期2卷 121-133页

作者： Víctor de Carvalho Martins Ronoel Luiz de Oliveira Godoy Ana Cristina miranda Senna Gouvêa Manuela Cristina Pessanha de araujo Santiago Renata Galhardo Borguini Elaine Cristina de Oliveira Braga Sidney Pacheco Luzimar da Silva de Mattos do Nascimento departamento de Tecnologia de Alimentos Instituto de TecnologiaUniversidade Federal Rural do Rio de JaneiroCEP 23890-000Seropédica-RJBrazil Laboratório de Cromatografia Líquida Embrapa Agroindústria de AlimentosCEP 23020-470Rio de Janeiro-RJBrazil Centro de Tecnologia Instituto de QuímicaUniversidade Federal do Rio de JaneiroCEP 22290-180Rio de Janeiro-RJBrazil

Coffee is currently the second largest commodity on the world market today,and there is great concern about the quality of the beans exported from producer countries to Europe and *** such as using blends of different species and adding low-cost raw materials,such as chicory,corn,and soybean,impair the sensory and functional characteristics of the drink made from roasted and ground coffee *** is a need to adopt more efficient analytical methods than the microscopy technique currently *** first chromatographic method used to determine fraud was reported in *** method used paper chromatography to differentiate between coffee and chicory based on the free reducing *** of the 1980s,different methods involving high-performance liquid chromatography and gas chromatography were developed in order to demonstrate geographic authenticity,distinction between species,occurrence of adulteration,and the presence of defective beans by determining the monosaccharides,oligosaccharides,tocopherols,fatty acids,volatiles,diterpenes,sterols,and phenolic substances,among *** far as the authors know,there are no papers published in the literature that have compiled such an extensive set of information about these chromatographic methods as *** the last 2 years,there has been a trend to develop analytical methods for ultra-performance liquid chromatography coupled with tandem mass spectrometry to confirm fraud in coffee,due to high sensitivity and selectivity.

关键词： adulteration coffee chromatographic methods carbohydrates volatile substances

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Candida albicans isolated from urine:Phenotypic and molecular identification,virulence factors and antifungal susceptibility

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Asian Pacific Journal of Tropical Biomedicine 2017年第7期7卷 624-628页

作者： Laura Wiebusch Adriana Araújo de Almeida-Apolonio Luana Mireli Carbonera Rodrigues Bruna de Paula Bicudo Danielly Beraldo dos Santos Silva danielle Ferreira Lonchiati Renata Pires de araujo Alexéia Barufatti Grisolia Kelly Mari Pires de Oliveira Faculdade de Ciencias Biologicas e Ambientais Universidade Federal da Grande DouradosDouradosMSBrazil Faculdade de Medicina Universidade Federal de Mato Grosso do SulCampo GrandeMSBrazil Faculdade de Ciencias da Saude Universidade Federal da Grande DouradosDouradosMSBrazil Faculdade de Ciencias Agrarias e Veterinarias Universidade Estadual Paulista“Julio de Mesquita Filho”JaboticabalSPBrazil

Objective: To isolate Candida albicans(C. albicans) from the urine of hospitalized patients and assess the virulence factors and antifungal susceptibility profiles of the ***: Yeasts were identified using the chromogenic medium CHROMagar?, the VITEK~?2 system, hypertonic Sabouraud broth, tobacco agar, polymerase chain reaction,and DNA sequencing. The evaluated virulence factors were proteinase production,phospholipase production, and biofilm production on polystyrene. The broth microdilution technique was used to determine the minimum inhibitory ***: All yeasts isolated from urine were identified as C. albicans using both classical and molecular methods. Although 91.3% of the isolates showed no phospholipase activity, 56.5% showed strong proteinase activity and 91.7% produced biofilm. All microorganisms were sensitive to fluconazole, voriconazole and amphotericin B, but 56.5% of the yeasts showed resistance to ***: C. albicans isolates from urine have a high capacity for virulence and can be associated with infectious processes. Furthermore, the high percentage of isolates resistant to itraconazole is important because this antifungal agent is commonly used to treat fungal infections in the hospital environment.

关键词： Candidiasis Urinary tract Infection Virulence Antifungal susceptibility

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Current status and future perspectives of radiomics in hepatocellular carcinoma

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World Journal of Gastroenterology 2023年第1期29卷 43-60页

作者： Joao miranda Natally Horvat Gilton Marques Fonseca Jose de Arimateia Batista araujo-Filho Maria ClaraFernandes Charlotte Charbel Jayasree Chakraborty Fabricio Ferreira Coelho Cesar Higa Nomura Paulo Herman department of Radiology University of Sao PauloSao Paulo 05403-010Brazil department of Radiology Memorial Sloan-Kettering Cancer CenterNew YorkNY 10065United States department of Gastroenterology University of Sao PauloSao Paulo 05403-000Brazil department of Radiology Hospital Sirio-LibanesSao Paulo 01308-050Brazil department of Surgery Memorial Sloan-Kettering Cancer CenterNew YorkNY 10065United States department of Radiology University of Sao PauloSao Paulo 05403-000Brazil

Given the frequent co-existence of an aggressive tumor and underlying chronic liver disease,the management of hepatocellular carcinoma(HCC)patients requires experienced multidisciplinary team ***,imaging plays a key role in the diagnosis,staging,restaging,and surveillance of ***,imaging assessment of HCC entails the assessment of qualitative characteristics which are prone to inter-reader *** is an emerging field that extracts high-dimensional mineable quantitative features that cannot be assessed visually with the naked eye from medical *** main potential applications of radiomic models in HCC are to predict histology,response to treatment,genetic signature,recurrence,and *** the encouraging results to date,there are challenges and limitations that need to be overcome before radiomics implementation in clinical *** purpose of this article is to review the main concepts and challenges pertaining to radiomics,and to review recent studies and potential applications of radiomics in HCC.

关键词： Radiomics Hepatocellular carcinoma Texture analysis Radiology

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Peroxisome proliferator-activated receptors as targets to treat metabolic diseases:Focus on the adipose tissue,liver,and pancreas

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World Journal of Gastroenterology 2023年第26期29卷 4136-4155页

作者： Henrique Souza-Tavares Carolline Santos miranda Isabela Macedo Lopes Vasques-Monteiro Cristian Sandoval Daiana araujo Santana-Oliveira Flavia Maria Silva-Veiga Aline Fernandes-da-Silva Vanessa Souza-Mello department of Anatomy Rio de Janeiro State UniversityRio de Janeiro 20551030Brazil Escuela de Tecnología Médica Facultad de SaludUniversidad Santo TomásOsorno 5310431Chile departamento de Ciencias Preclínicas Universidad de la FronteraTemuco 4780000Chile

The world is experiencing reflections of the intersection of two pandemics:Obesity and coronavirus disease *** prevalence of obesity has tripled since 1975 worldwide,representing substantial public health costs due to its *** adipose tissue is the initial site of obesity *** excessive energy intake,it undergoes hyperplasia and hypertrophy until overt inflammation and insulin resistance turn adipocytes into dysfunctional cells that send lipotoxic signals to other *** pancreas is one of the organs most affected by *** lipotoxicity becomes chronic,there is an increase in insulin secretion by pancreatic beta cells,a surrogate for type 2 diabetes mellitus(T2DM).These alterations threaten the survival of the pancreatic islets,which tend to become dysfunctional,reaching exhaustion in the long *** for the liver,lipotoxicity favors lipogenesis and impairs beta-oxidation,resulting in hepatic *** silent disease affects around 30%of the worldwide population and can evolve into end-stage liver *** therapy for hepatic steatosis remains to be defined,peroxisome proliferator-activated receptors(PPARs)activation copes with T2DM *** PPARs are transcription factors found at the intersection of several metabolic pathways,leading to insulin resistance relief,improved thermogenesis,and expressive hepatic steatosis mitigation by increasing mitochondrial *** review aimed to update the potential of PPAR agonists as targets to treat metabolic diseases,focusing on adipose tissue plasticity and hepatic and pancreatic remodeling.

关键词： Obesity Insulin resistance Peroxisome proliferator-activated receptors Pancreas Hepatic steatosis Adipose tissue

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Bacterial Fecal Microbiota in Healthy Subjects and Inpatients with <i>Clostridium difficile</i>Infection

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Advances in Microbiology 2017年第1期7卷 10-21页

作者： Thaís Flecher Barbosa danielle Angst Secco Raquel Silva Peixoto Alexandre Soares Rosado Flavia Lima do Carmo Hugo Emiliano de Jesus Leandro araujo Lobo Luis Caetano Martha Antunes Karla Rodrigues miranda Geraldo Renato de Paula Regina Maria Cavalcanti Pilotto Domingues Instituto de Microbiologia Paulo de Goes Universidade Federal do Rio de Janeiro Rio de Janeiro RJ Brazil Escola Nacional de Saúde Pública Sergio Arouca Fundacao Oswaldo Cruz Rio de Janeiro RJ Brazil Faculdade de Farmácia Universidade Federal do Rio de Janeiro—Campus Macaé Macaé RJ Brazil departamento de Tecnologia Farmacêutica Universidade Federal Fluminense Niterói RJ Brazil

Clostridium difficile is a nosocomial enteric pathogen, considered the main etiological agent of antibiotic-associated diarrhea in hospitals. C. difficile is resistant to several antimicrobial agents used in empiric treatment protocols, which confers selective advantages to this species as compared with other members of the intestinal microbiota. The aim of this study was to evaluate and identify possible alterations of the intestinal microbiota population resulting from the use of antimicrobials associated with infections by C. difficile (CDI). denaturing Gradient Gel Electrophoresis (DGGE) was used to evaluate fecal samples from two groups: healthy subjects and patients with C. difficile-associated diarrhea (CDAD). A decrease in bacterial diversity was highlighted by the low number of bands in samples from CDAD patients, compared with healthy subjects. This may indicate that antibiotic treatment would affect bacterial diversity, leading to a significant difference between the intestinal microbiota of these two groups, but further studies are still needed. Firmicutes and Verrucomicrobia phyla were detected mainly in healthy individuals, and these could be related to protection factors against the CDI. Klebsiella variicola/K. pneumoniae were found mostly in samples from CDI patients. This study shows the effects of antimicrobials and the CDI itself on human intestinal microbiota.

关键词： Clostridium difficile DGGE Microbiota CDAD Antimicrobials

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