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The transcription factor ZEB1 promotes chemoresistance in prostate cancer cell lines

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Asian Journal of Andrology 2019年第5期21卷 460-467页

作者： Octavio Orellana-Serradell daniela Herrera Enrique A Castellon Hector R Contreras Department of Basic and Clinical Oncology Faculty of MedicineUniversity of ChileIndependenciaSantiago 8380453Chile

One of the factors promoting tumoral progress is the abnormal activation of the epithelial-mesenchymal transition(EMT)program which has been associated with chemoresistance in tumoral *** transcription factor zinc finger E-box-binding homeobox 1(ZEB1),a key EMT activator,has recently been related to docetaxel resistance,the main chemotherapeutic used in advanced prostate cancer *** mechanisms involved in this protective effect are still *** a previous work,we demonstrated that ZEB1 expression induced an EMT-like phenotype in prostate cancer cell *** this work,we used prostate cancer cell lines 22Rvl and DU145 to study the effect of ZEB1 modulation on docetaxel resistance and its possible *** results showed that ZEB1 overexpression conferred to 22Rvl cell resistance to docetaxel while its silencing made DU145 cells more sensitive to *** of resistance markers showed no presenee of ATP-binding cassette subfamily B member 1(MDR1)and no changes in breast cancer resistance protein(BCRP)or ATP-binding cassette subfamily C member 10(MRP7).However,a correlation between ZEB1,multidrug resistance-associated protein 1(MRP1),and ATP-binding cassette subfamily C member 4(MRP4)expression was ***4 inhibition,using MK571,resensitized cells with ZEB1 overexpression to docetaxel *** addition,modulation of ZEB1 and subsequent change in MRP4 expression correlated with a lower apoptotic response to docetaxel,characterized by lower B-cell lymphoma 2(Bcl2),high BCL2-associated X protein(Bax),and high active caspase 3 *** response to docetaxel in our model seems to be mediated mainly by activation of the apoptotic death *** results showed that modulation of MRP4 could be a mediator of ZEBl-related resistance to docetaxel in prostate cancer,making it a possible marker for chemotherapy response in patients who do not express MDR1.

关键词： chemoresistance prostate cancer ZEB1

Enhance Stability and in vitro Cell Response to a Bioinspired Coating on Zr Alloy with Increasing Chitosan Content

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Journal of Bionic Engineering 2017年第3期14卷 459-467页

作者： daniela Ionita Maria Vardaki Miruna S. Stan Anca Dinischiotu Ioana Demetrescu Faculty of Applied Chemistry and Materials Science University Politehnica of Bucharest 1-7 Polizu 011061 Bucharest Romania Department of Biochemistry and Molecular Biology Faculty of Biology University of Bucharest 91-95 Splaiullndependentei 050095 Bucharest Romania Academy of Romanian Scientist Splaiullndependentei 54 050094 Bucharest Romania

The aim of the present paper is to characterize bioinspired chitosan （CS）＋ hydroxyapatite （HA） coatings with various components ratio on a zirconium alloy with titanium. The coatings were characterized by FT-IR, SEM, hydrophilic/hydrophobic balance, adherence, roughness, electrochemical stability and in vitro cell response. Electrochemical tests, including potentio- dynamic polarization curves and electrochemical impedance spectroscopy, were performed in normal saline physiological solution. Cell viability of MC3T3-E1 osteoblasts, lactate dehydrogenase, nitric oxide, and Reactive Oxygen Species （ROS） levels, as well as actin cytoskeleton morphology, were evaluated as biological in vitro tests. The results on in vitro cell response indicated good cell membrane integrity and viability for all samples, but an increased cell number, a decreased ROS level and a better cytoskeleton organization were noticed for the sample with a higher CS content. The coating with highest CS concen- tration indicated the best performance based on the experimental data. The highest hydrophilic character, highest resistance to corrosion and best biocompatibility as well recommend this coating for bioapplications in tissue engineering.

关键词： ZrTi alloy bioinspired coating cells response electrochemical stability

Muckle-Wells syndrome: manifestations and diagnosis in four generations of a Portuguese family

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World Journal of Pediatrics 2020年第5期16卷 541-542页

作者： Soraia Raquel Loureiro Azevedo daniela Filipa Marinho Peixoto Rheumatology Department Unidade Local de Satúde Do Alto MinhoHospital Conde Bertiandos4990-041Ponte de LimaPortugal

Several medical conditions can present as fever and rash in children,including infections,hypersensitivity reactions and immune-mediated diseases[1].Cryopyrin-associated periodic syndrome(CAPS)is a group of inherited autoinflammatory disorders caused by gain-of-function mutations in the NLRP3 gene(CIAS 1).CAPS encompass a spectrum of diseases including Muckle-Wells syndrome(MWS).The A439V mutation is considered a disease-causing mutation,although clinical phenotypes can be diverse[2].MWS is characterized by recurrent episodes of fever,urticarial rash,arthralgia/arthritis,and more rarely,oral ulcers,folliculitis,or erythema nodosum[3].

关键词： diagnosis CAPS fever

The transcription factor ZEB1 promotes an aggressive phenotype in prostate cancer cell lines

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Asian Journal of Andrology 2018年第3期20卷 294-299页

作者： Octavio Orellana-Serradell daniela Herrera Enrique A Castellon Hector R Contreras Department of Basic and Clinical Oncology Faculty of Medicine University of Chile Independencia Santiago 8380453 Chile.

It has been reported that one of the factors that promotes tumoral progression is the abnormal activation of the epithelial-mesenchymal transition program. This process is associated with tumoral cells acquiring invasive and malignant properties and has the transcription factor zinc finger E-box-binding homeobox 1 （ZEB1） as one of its main activators. However, the role of ZEB1 in promoting malignancy in prostate cancer （PC, a） is still unclear. Here, we report that ZEB1 expression correlates with Gleason score in PCa samples and that expression of ZEB1 regulates epithelial-mesenchymal transition and malignant characteristics in PCa cell lines. The results showed that ZEB1 expression is higher in samples of higher malignancy and that overexpression of ZEB1 was able to induce epithelial-mesenchymal transition by upregulating the mesenchymal marker Vimentin and downregulating the epithelial marker E-Cadherin. On the contrary, ZEB 1 silencing repressed Vimentin expression and upregulated E-Cadherin. ZEB1 expression conferred enhanced motility and invasiveness and a higher colony formation capacity to 22Rvl cells whereas DU145 cells with ZEB1 silencing showed a decrease in those same properties. The results showed that ZEB1 could be a key promoter of tumoral progression toward advanced stages of PC, a.

关键词： epithelial-mesenchymal transition prostate cancer transcriptional repression ZEB1

A Probabilistic Approach to the Evaluation of Seismic Resilience in Road Asset Management

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International Journal of Disaster Risk Science 2022年第1期13卷 114-124页

作者： Vittorio Nicolosi Maria Augeri Mauro D'Apuzzo Azzurra Evangelisti daniela Santilli Department of Enterprise Engineering“Mario Lucertini” University of Rome“Tor Vergata”00133 RomeItaly Department of Civil and Mechanical Engineering University of Cassino and Southern Lazio03043 CassinoItaly

Road networks are classified as critical infrastructure *** loss of functionality not only hinders residential and commercial activities,but also compromises evacuation and rescue after *** with risks to key strategic objectives is not new to asset management,and risk management is considered one of the core elements of asset *** analysis has recently focused on understanding and designing strategies for resilience,especially in the case of seismic events that present a significant hazard to highway transportation *** a review of risk and resilience concepts and metrics,an innovative methodology to stochastically assess the economic resources needed to restore damaged infrastructures,one that is a relevant and complementary element within a wider resilience-based framework,is *** original methodology is based on collecting and analyzing ex post reconstruction and hazard data and was calibrated on data measured during the earthquake that struck central Italy in 2016 and collected in the following recovery *** further improvements are needed,the proposed approach can be used effectively by road managers to provide useful information in developing seismic retrofitting plans.

关键词： Italy Resilience metrics Road asset management Road network resilience Seismic hazards

Methodology and evidence from a case study in Rome to increase pedestrian safety along home-to-school routes

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Journal of Traffic and Transportation Engineering(English Edition) 2020年第5期7卷 715-727页

作者： Maria Vittoria Corazza daniela D'Alessandro Paola Di Mascio Laura Moretti Department of Civil Building and Environmental EngineeringSapienza University of RomeRome 00185Italy

Home-to-school routes are very sensitive areas: they represent, for children, a learning tool for their everyday activities, but if poorly designed, maintained and equipped they can expose them to traffic risks. Sidewalks' inappropriate level of service and poor maintenance, especially, are main factors contributing to walking unsuitability, thus to poor comfort and safety levels for young pedestrians, and more in general for all the vulnerable non-motorized road users. This paper deals with a methodology specifically developed to highlight the quality of the urban environment where the home-to-school routes are located, according to four main criteria: wellbeing, usage, appearance, and safety and security. Each criterion is associated with a checklist including the most relevant features to assess, with a focus on maintenance as a key parameter to create safe and comfortable routes to school. An application, a case study in Rome, where the lack of regular maintenance results into a network of unsafe sidewalks, is also presented, analyzing three different areas where a number of schools are located. Detours when approaching school premises were surveyed, due to the levels of distresses and linked to the poor comfort and safety levels. As the mutual influence of built environment over road safety and maintenance requirements for home-to-school paths is not largely investigated thus far, the paper's goal is to provide advanced knowledge for studies and applications further afield.

关键词： Traffic engineering Road safety Pedestrians Home-to-school routes Sidewalks

Neglected features of lifestyle: Their relevance in nonalcoholic fatty liver disease

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World Journal of Hepatology 2016年第33期8卷 1459-1465页

作者： Francesca M Trovato Giuseppe Fabio Martines daniela Brischetto Guglielmo Trovato daniela Catalano Department of Clinical and Experimental Medicine Postgraduate School of Clinical Echo-graphythe University of Catania Clinical Research and Innovation Project Planning Unit the School of Medicine and AOU Policlinicothe University of Catania Department of Medicine the School of Medicine of the University of Catania

AIMTo investigated in non-alcoholic-fatty-liver-disease (NAFLD), with ultrasound (US)-detected fatty liver, and in a group of non-alcoholic and otherwise healthy subjects, relationship of neglected features of lifestyle with NAFLD and obesity. METHODSFive hundred and thirty-two NAFLD and 667 non-NAFLD healthy subjects, age 21-60 years were studied. Severity of liver steatosis was assessed by US bright liver score. The adherence to mediterranean diet score (AMDS) was assessed on the basis of a 1-wk recall computerized questionnaire which included a detailed physical activity reports (Baecke questionnaire). The western dietary profile score, as a simplified paradigm of unhealthy diet, a questionnaire quantifying sun exposure score and a sleep habits questionnaires provided a further comprehensive lifestyle assessment. RESULTSBody mass index (BMI), insulin resistance (HOMA), and triglycerides, poorer adherence to a mediterranean diet profile, sedentary habits, minor sun exposure and use of “western diet” foods are greater in NAFLD. Multiple linear regression analysis, weighted by years of age, displays BMI, HOMA and AMDS as the most powerful independent predictors of fatty liver severity; however, also the physical activity score, the western diet habit and the sun exposure score are acting inside the model with significant independent effects. CONCLUSIONArticulated clinical intervention, according to our results, are justified in NAFLD and can be pursued addressing by focused intervention nutritional profile, physical exercise mainly in open-air subsets for enhancing sun exposure and healthier sleep duration and rhythm.

关键词： Fatty liver Ultrasound Diet Malnutrition Sleep Clinical risk management Health psychology Sun exposure Obesity

Possible markers of the Jurassic/Cretaceous boundary in the Mediterranean Tethys:A review and state of art

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Geoscience Frontiers 2011年第4期2卷 475-490页

作者： Jozef Michalik daniela Rehkov Slovakian Academy of Sciences Geological InstituteDubravska cesta 9P.O.Box 106840 05 BratislavaSlovak Republic Comenius University Faculty of Natural SciencesDepartment of Geology and PalaeontologyMlynska dolina G-1842 15 BratislavaSlovak Republic

During the last decades,several integrated studies of Tethyan Jurassic/Cretaceous boundary sections from different countries were published with the objective to indicate problems for the selection of biological,chemical or physical markers suitable for identification of the Jurassic/Cretaceous boundary-the only system boundary within the Phanerozoic still not fixed by *** the boundary between the Jurassic and Cretaceous systems is a matter of global scale *** problem of proposing possible J/K boundary stratotypes results from lack of a global index fossils, global sea level drop,paleogeographic changes causing development of isolated facies areas,as well as from the effect of Late Cimmerian *** contribution summarizes and comments data on J/K boundary interval obtained from several important Tethyan sections and shows still existing problems and discrepancies in its determination.

关键词： J/K boundary Interregional correlation Biostratigraphic proxies Calpionellids Nannofossils

Pathological features of tissues and cell populations during cancer cachexia

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Cell Regeneration 2022年第1期11卷 146-162页

作者： daniela Di Girolamo Shahragim Tajbakhsh Stem Cells&Development Department of Developmental&Stem Cell BiologyInstitut Pasteur75015 ParisFrance UMR 3738 CNRSInstitut Pasteur75015 ParisFrance

Cancers remain among the most devastating diseases in the human population in spite of considerable advances in limiting their impact on lifespan and *** multifactorial nature of cancers,as well as the number of tissues and organs that are affected,have exposed a considerable diversity in mechanistic features that are reflected in the wide array of therapeutic strategies that have been *** is manifested in a number of diseases ranging from cancers to diabetes and *** the context of cancers,a majority of patients experience cachexia and succumb to death due to the indirect effects of tumorigenesis that drain the energy reserves of different *** information is available on the pathophysiological features of cancer cachexia,however limited knowledge has been acquired on the resident stem cell populations,and their function in the context of these *** we review current knowledge on cancer cachexia and focus on how tissues and their resident stem and progenitor cell populations are individually affected.

关键词： Cancer cachexia Stem cells Tissue wasting