Enhance Stability and in vitro Cell Response to a Bioinspired Coating on Zr Alloy with Increasing Chitosan Content
Enhance Stability and in vitro Cell Response to a Bioinspired Coating on Zr Alloy with Increasing Chitosan Content作者机构：Faculty of Applied Chemistry and Materials Science University Politehnica of Bucharest 1-7 Polizu 011061 Bucharest Romania Department of Biochemistry and Molecular Biology Faculty of Biology University of Bucharest 91-95 Splaiullndependentei 050095 Bucharest Romania Academy of Romanian Scientist Splaiullndependentei 54 050094 Bucharest Romania
出 版 物：《Journal of Bionic Engineering》 (仿生工程学报（英文版）)
年 卷 期：2017年第14卷第3期
页 面：459-467页
核心收录：
学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 07[理学] 08[工学] 080502[工学-材料学] 0805[工学-材料科学与工程（可授工学、理学学位）] 070102[理学-计算数学] 0836[工学-生物工程] 0701[理学-数学] 0702[理学-物理学]
主 题：ZrTi alloy bioinspired coating cells response electrochemical stability
摘 要：The aim of the present paper is to characterize bioinspired chitosan （CS） ＋ hydroxyapatite （HA） coatings with various components ratio on a zirconium alloy with titanium. The coatings were characterized by FT-IR, SEM, hydrophilic/hydrophobic balance, adherence, roughness, electrochemical stability and in vitro cell response. Electrochemical tests, including potentio- dynamic polarization curves and electrochemical impedance spectroscopy, were performed in normal saline physiological solution. Cell viability of MC3T3-E1 osteoblasts, lactate dehydrogenase, nitric oxide, and Reactive Oxygen Species （ROS） levels, as well as actin cytoskeleton morphology, were evaluated as biological in vitro tests. The results on in vitro cell response indicated good cell membrane integrity and viability for all samples, but an increased cell number, a decreased ROS level and a better cytoskeleton organization were noticed for the sample with a higher CS content. The coating with highest CS concen- tration indicated the best performance based on the experimental data. The highest hydrophilic character, highest resistance to corrosion and best biocompatibility as well recommend this coating for bioapplications in tissue engineering.
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