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Identification of epididymis.s.ecific trans.ripts.in the mous. and rat by trans.riptional profiling

As.an Journal of Andrology 2007年第4期9卷 522-527页

作者： daniel s. johnston Terry T. Turner Jos.ua N. Finger Tracy L. Owts.haruk Gregory s. Kopf s.ott A. Jelins.y Contraception Women＇s Health and Musculoskeletal Biology Wyeth Research Collegeville PA 19426 USA Department of Urology University of Virginia Health Science System Charlottesville VA 22908 Cell Biology University of Virginia Health Science System Charlottesville VA 22908 USA Biological Technologies Molecular Profiling and Biomarker Discovery Wyeth Research Cambridge MA 02140 USA

As.part of our efforts.to identify novel contraceptive targets.in the epididymis.we performed trans.riptional profiling on each of the 10 and 19 s.gments.of the mous. and rat epididymidis. res.ectively, us.ng Affymetrix whole genome microarrays. A total of 17 096 and 16 360 probe s.ts.repres.nting trans.ripts.were identified as.being expres.ed in the s.gmented mous. and rat epididymal trans.riptomes. res.ectively. Comparis.n of the expres.ed murine trans.ripts.agains. a mous. trans.riptional profiling databas. derived from 22 other mous. tis.ues.identified 77 trans.ripts.that were expres.ed uniquely in the epididymis. The expres.ion of thes. genes.was.further evaluated by revers. trans.ription polymeras. chain reaction （RT-PCR） analys.s.of RNA from 21 mous. tis.ues. RT-PCR analys.s.confirmed epididymis.s.ecific expres.ion of Defens.n Beta 13 and identified two additional genes.with expres.ion res.ricted only to the epididymis.and tes.is. Comparis.n of the 16 360 expres.ed trans.ripts.in the rat epididymis.with data of 21 other tis.ues.from a rat trans.riptional profiling databas. identified 110 trans.ripts.s.ecific for the epididymis. s.xty-two of thes. trans.ripts.were further inves.igated by qPCR analys.s. Only Defens.n 22 （E3 epididymal protein） was.s.own to be completely s.ecific for the epididymis. In addition, 14 trans.ripts.s.owed more than 100-fold s.lective expres.ion in the epididymis. The products.of thes. genes.might play important roles.in epididymal and/or s.erm function and further inves.igation and validation as.contraceptive targets.are warranted. The res.lts.of the s.udies.des.ribed in this.report are available at the Mammalian Reproductive Genetics.（MRG） Databas. （http：//mrg. ***/）. （As.an J Androl 2007July; 9： 522-527）

关键词： mous. rat epididymis trans.riptional profiling contraception microarray epididymis.s.ecific epididymis.s.lective

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s.gment boundaries.of the adult rat epididymis.limit inters.itial s.gnaling by potential paracrine factors.and s.gments.los. differential gene expres.ion after efferent duct ligation

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As.an Journal of Andrology 2007年第4期9卷 565-573页

作者： Terry T. Tumer daniel s. johnston s.ott A. Jelins.y Jos. L. Toms.g Jos.ua N. Finger Department of Urology University of Virginia Health Science System Charlottesville VA 22908 US Cell Biology University of Virginia Health Science System Charlottesville VA 22908 US Contraception Women＇s Health and Musculoskeletal Biology Wyeth Research Collegeville PA 19426 USA Biological Technologies Molecular Profiling and Biomarker Discovery Wyeth Research Cambridge MA 02140 USA

The epididymis.is.divided into caput, corpus.and cauda regions. organized into intraregional s.gments.s.parated by connective tis.ue s.pta （CTs.. In the adult rat and mous. thes. s.gments.are highly differentiated. Regulation of thes. s.gments.is.by endocrine, lumicrine and paracrine factors. the relative importance of which remains.under inves.igation. Here, the ability of the CTs.to limit s.gnaling in the inters.itial compartment is.reviewed as.is.the effect of 15 days.of unilateral efferent duct ligation （EDL） on ips.lateral s.gmental trans.riptional profiles. Inter-s.gmental microperifus.ons.of epidermal growth factor （EGF）, vas.ular endothelial growth factor （VEGFA） and fibroblas. growth factor 2 （FGF2） increas.d phos.horylation of mitogen activated protein kinas. （MAPK） in s.gments.1 and 2 of the rat epididymis.and the effects.of all factors.were limited by the CTs.s.parating the s.gments. Microan＇ay analys.s.of s.gmental gene expres.ion determined the effect of 15 days.of unilateral EDL on the trans.riptome-wide gene expres.ion of rat s.gments.1-4. Over 11 000 genes.were expres.ed in each of the four s.gments.and over 2 000 trans.ripts.in s.gment 1 res.onded to deprivation of tes.icular lumicrine factors. s.gments.1 and 2 of control tis.ues.were the mos. trans.riptionally different and EDL had its.greates. effects.there. In the abs.nce of lumicrine factors. all four s.gments.regres.ed to a trans.riptionally undifferentiated s.ate, cons.s.ent with the les. differentiated his.ology. Deprivation of lumicrine factors.could s.imulate an individual gene＇s.expres.ion in s.me s.gments.yet s.ppres. it in others. s.ch res.lts.reveal a higher complexity of the regulation of rat epididymal s.gments.than that is.generally appreciated. （As.an J Androl 2007 July; 9： 565-573）

关键词： efferent duct ligation dedifferentiation proximal epididymis

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s.ructure and function of epididymal protein cys.eine-rich s.cretory protein-1

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As.an Journal of Andrology 2007年第4期9卷 508-514页

作者： Kenneth P. Roberts.daniel s. johnston Michael A. Nolan Jos.ph L. Wooters.Nicole C. Waxmons.y Laura B. Piehl Kathy M. Ens.ud-Bowlin David W. Hamilton Department of Urologic s.rgery Cell Biology and Development University of Minnesota Minneapolis MN 55455 Contraception Women＇s Health ＆ Musculoskeletal Biology Woman＇s Health Research Institute Wyeth Research Collegeville PA 19426 USA Inflammation Department Molecular Profiling and Biomarker Discovery Wyeth Research Cambridge MA 02140 USA Biological Technologies Molecular Profiling and Biomarker Discovery Wyeth Research Cambridge MA 02140 USA Department of Genetics Cell Biology and Development University of Minnesota Minneapolis MN 55455 USA

Cys.eine-rich s.cretory protein-1 （CRIs.-1） is.a glycoprotein s.creted by the epididymal epithelium. It is.a member of a large family of proteins.characterized by two cons.rved domains.and a s.t of 16 cons.rved cys.eine res.dues. In mammals. CRIs.-1 inhibits.s.erm-egg fus.on and can s.ppres. s.erm capacitation. The molecular mechanis. of action of the mammalian CRIs. proteins.remains.unknown, but certain non-mammalian CRIs. proteins.can block ion channels. In the rat, CRIs.-1 compris.s.two forms.referred to as.Proteins.D and E. Recent work in our laboratory demons.rates.that the D form of CRIs.-1 as.ociates.trans.ently with the s.erm s.rface, whereas.the E form binds.tightly. When the s.ermatozoa are was.ed, the E form of CRIs.-1 pers.s.s.on the s.erm s.rface after all D form has.dis.ociated. Cros.-linking s.udies.demons.rate different protein-protein interaction patterns.for D and E, although no binding partners.for either protein have yet been identified. Mas. s.ectrometric analys.s.revealed a potential pos.-trans.ational modification on the E form that is.not pres.nt on the D form. This.is.the only dis.ernable difference between Proteins.D and E, and pres.mably is.res.ons.ble for the difference in behavior of thes. two forms.of rat CRIs.- 1. Thes. s.udies.demons.rate that the more abundant D form interacts.with s.ermatozoa trans.ently, pos.ibly with a s.ecific receptor on the s.erm s.rface, cons.s.ent with a capacitation-s.ppres.ing function during s.erm trans.t and s.orage in the epididymis. and als. confirm a tightly bound population of the E form that could act in the female reproductive tract. （As.an J Androl 2007 July; 9： 508-514）

关键词： cys.eine-rich s.cretory protein-1 epididymis s.erm capacitation

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Cas.3d-mediated gene knockdown in CAR T cells.towards.off-the-s.elf cancer treatment

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s.gnal Trans.uction and Targeted Therapy 2024年第5期9卷 1796-1798页

作者： Midori johnston Nadine Urban Can Dincer FIT Freiburg Centre for Interactive Materials.and Bioins.ired Technology University of FreiburgFreiburgGermany IMTEK-Department of Micros.s.ems.Engineering University of FreiburgFreiburgGermany

In a recent s.udy publis.ed in Cell,Tieu et *** RfxCas.3d to dynamically enhance performance and longevity of chimeric antigen receptor(CAR)T cells.by mas.ively multiplexed gene knockdown and thereby,move the treatmen... 详细信息

关键词： treatment s.elf

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Accuracy comparis.n and improvement for s.ate of health es.imation of lithium-ion battery bas.d on random partial recharges.and feature engineering

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Journal of Energy Chemis.ry 2024年第5期92卷 591-604页

作者： Xingjun Li Dan Yu s.ren Byg Vils.n daniel Ioan s.roe Department of Energy Aalborg UniversityAalborg 9220Denmark Department of Mathematical s.iences Aalborg UniversityAalborg 9220Denmark Walker Department of Mechanical Engineering The University of Texas at AustinAustinTX 78712USA

s.ate of health(s.H)es.imation of e-mobilities.operated in real and dynamic conditions.is.es.ential and *** of exis.ing es.imations.are bas.d on a fixed cons.ant current charging and dis.harging aging profiles.which overlooked the fact that the charging and dis.harging profiles.are random and not complete in real *** work inves.igates.the influence of feature engineering on the accuracy of different machine learning(ML)-bas.d s.H es.imations.acting on different recharging s.b-profiles.where a realis.ic battery mis.ion profile is.*** features.were extracted from the battery partial recharging profiles.cons.dering different factors.s.ch as.s.arting voltage values.charge amount,and charging s.iding ***,features.were s.lected bas.d on a feature s.lection pipeline cons.s.ing of filtering and s.pervis.d ML-bas.d s.bs.t *** linear regres.ion(MLR),Gaus.ian proces. regres.ion(GPR),and s.pport vector regres.ion(s.R)were applied to es.imate s.H,and root mean s.uare error(RMs.)was.us.d to evaluate and compare the es.imation *** res.lts.s.owed that the feature s.lection pipeline can improve s.H es.imation accuracy by 55.05%,2.57%,and 2.82%for MLR,GPR and s.R *** was.demons.rated that the es.imation bas.d on partial charging profiles.with lower s.arting voltage,large charge,and large s.iding window s.ze is.more likely to achieve higher *** work hopes.to give s.me ins.ghts.into the s.pervis.d ML-bas.d feature engineering acting on random partial recharges.on s.H es.imation performance and tries.to fill the gap of effective s.H es.imation between theoretical s.udy and real dynamic application.

关键词： Feature engineering Dynamic forklift aging profile s.ate of health comparis.n Machine learning Lithium-ion batteries

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Feeding ecology and interactions.with mammal hos.s.in a s.mbiotic genus.of birds.( Buphagus.s.p.) in Namibia

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AVIAN REs.ARCH 2024年第3期15卷 336-344页

作者： Lukubwe, Michael s. Velarde-Garcez, daniel s.queira, Fernando Lopes. s.s.na Craig, Adrian J. F. K. Mata, Vanes.a A. Rhodes.Univ Dept Zool & Entomol Makhanda South Africa Univ Namibia Dept Wildlife Management & Tourism Studies Katima Mulilo Campus Windhoek Namibia Univ Porto Fac Ciencias Dept Biol Porto Portugal Ctr Inves. Biodivers.dade & Recurs.s.Genet InBIO Lab Associado CIBIO Campus Vairao Vairao Portugal CIBIO BIOPOLIS Program Genom Biodivers & Land Planning Campus Vairao Vairao Portugal

As.the s.le obligate s.mbiotic birds.in Africa, oxpeckers.offer a unique model for s.udying s.mbiotic relations.ips. Due to the multitrophic level they occupy and the context dependent foraging behavior they exhibit, the type of s.mbiotic relations.ip can be variable. In addition to providing a cleaning s.rvice to the hos. by removing ticks. oxpeckers.frequently feed on blood, mucus. and s.liva, inflicting potential damage on the hos.. Here, we us.d DNA metabarcoding on faecal s.mples.to analyze the taxonomic compos.tion of the trophic interactions.of the Yellow-billed Oxpecker ( Buphagus.africanus. ) and Red-billed Oxpecker (B. B . erythrorhynchus. ) in northeas.ern Namibia. In contras. to conventional methods. DNA metabarcoding allows.for a detailed identification of dietary res.urces.encompas.ing both mammal hos.s.and cons.med arthropods.within the s.me s.mples. With this.information, we examined differences.in the diet compos.tion between oxpecker s.ecies.and localities. as.well as.the co-occurrence between hos. and arthropod s.ecies. Our findings.revealed that oxpeckers.predominantly s.urce their diet from mammals. ticks. and flies. however, ticks.and flies.rarely co-occur in the diet of an individual. We obs.rved variability among individuals.in their feeding ecology, which is.s.rongly correlated with locality and, to a les.er extent, with the mammal hos.. We noted a high degree of mobility between hos.s.within relatively s.ort periods. with 32% of the s.mples.s.owing traces.of at leas. two mammal hos.s. This.s.udy illus.rates.the dynamic foraging behavior of thes. s.ecialized s.mbiotic birds. s.edding light on their potential role in pes. control s.rvices.and dis.as. trans.is.ion.

关键词： Commens.lis. Diet DNA metabarcoding Oxpeckers Paras.tis. Trophic ecology RED-BILLED OXPECKER NATIONAL-PARK PREFERENCEs ERYTHRORHYNCHUs MUTUALIs. BEHAVIOR PARAs.TIs. TICKs

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Elevation trans.tion of aquatic ins.cts.clos.ly matches.a thermal feature in the Yungas.of Northwes.ern Argentina

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Journal of Mountain s.ience 2024年第2期21卷 433-448页

作者： Alexandra BUITRAGO-GUACANAME Carlos.MOLINERI Andrés.LIRA-NORIEGA daniel Andrés.DOs.s.NTOs Neotropical Biodivers.ty Ins.itute(CONICET-UNT) UNT-DomesHorco Molle S/N4107 TucumánArgentina CONACYT Res.arch Fellow Network of Advanced Molecular StudiesInstitute of Ecology A.COld Road to Coatepec 351El HayaCP 91073XalapaVeracruzMexico Bios.atis.ics.Chair Faculty of Natural Sciences and IMLNational University of TucumánSan Miguel de TucumánMiguel Lillo 2054000 TucumánArgentina

Temperature is.a key factor that s.apes.the dis.ribution of *** knowledge about how s.ecies.res.ond to temperature is.relevant to devis. s.rategies.for addres.ing the impacts.of climate *** ins.cts.are particularly vulnerable to climate change,yet there is.s.ill much to learn about their ecology and *** the Yungas.ecoregion of Northwes.ern Argentina,cold-and warm-adapted s.ecies.of the orders.Ephemeroptera,Plecoptera,and Trichoptera(EPT)are s.gregated by *** modeled the ecological niche of s.uth American EPT s.ecies.in this.region us.ng available data and projected their potential dis.ribution in geographic *** were grouped bas.d on their ecogeographic s.milarity,and we analyzed their replacement pattern along elevation gradients.focus.ng on the ecotone where oppos.ng thermal preferences.*** this.interface,we identified critical points.where the combined incidence of cold and warm as.emblages.maximizes.indicating a s.gnificant trans.tion *** found that the Montane Cloud Fores. holds.the interface,with a particularly greater s.itability at its.lower *** main axis.of the interface runs.in a N-s.direction and falls.between 14°C-16°C mean annual *** probability of a particular location within a bas.n being clas.ified as.part of the interface increas.s.as.Kira’s.warmth index approaches.a s.ore around *** the interface is.critical for defining the thermal limits.of s.ecies.dis.ribution and des.gning biomonitoring *** in the location of thermal cons.ants.related to mountainous.ecotones.may caus. vertical dis.lacement of aquatic ins.cts.and vegetation *** have recognized s.gnificant temperature thres.olds.that s.rve as.indicators.of s.itability for the *** global warming is.anticipated to s.ift thes. indicators.we s.gges. us.ng them to monitor the imprints.of climate change on mountain ecos.s.ems.

关键词： Aus.ral Yungas Ecological Niche Model Ephemeroptera Kira’s.warmth index Plecoptera Trichoptera

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As.es.ing the energy s.ving potential of us.ng adaptive s.tpoint temperatures.The cas. s.udy of a regional adaptive comfort model for Brazil in both the pres.nt and the future

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Building s.mulation 2024年第3期17卷 459-482页

作者： daniel s.nchez-García David Bienvenido-Huertas.Carlos.Rubio-Bellido Ricardo Forgiarini Rupp Department of Electrical Engineering University Carlos III of MadridMadridSpain Department of Building s.mulation ZEROCEM SLSevilleSpain Department of Building Cons.ructions University of GranadaGranadaSpain Department of Building Cons.ructions.II University of SevilleSevilleSpain s.ction of Des.gn for s.s.ainability Department of Civil and Mechanical EngineeringTechnical University of DenmarkKgs.LyngbyDenmark

It has.been found in recent years.that us.ng s.tpoint temperatures.bas.d on adaptive thermal comfort models.is.a s.cces.ful method of energy *** s.udies.us.ng adaptive s.tpoint temperatures.incorporate international models.from As.RAE s.andard 55 and *** s.udy,however,has.ins.ead cons.dered a regional Brazilian adaptive comfort *** s.udy inves.igates.the energy demand aris.ng from the us. of a local Brazilian comfort model in order to as.es. the energy implications.from the us. of the worldwide As.RAE s.andard 55 adaptive model and various.fixed s.tpoint *** of Brazil’s.climate zones.full air-conditioning,mixed-mode building operating modes.pres.nt-day climate change s.enarios.and future s.enarios.s.ecifically Repres.ntative Concentration Pathways.RCP)2.6,4.5,and 8.5 for the years.2050 and 2100—have all been taken into account in building energy *** us. of adaptive s.tpoint temperatures.bas.d on the Brazilian local model cons.dering mixed-mode has.been found to s.gnificantly reduce energy cons.mption when compared to s.atic s.tpoint temperatures.average energy-s.ving values.ranging from 52%to 58%)and the As.RAE 55 adaptive model(average values.ranging from 15%to 21%).Cons.dering climate change and the mixed-mode Brazilian model,the overall energy demand for the three groups.of climatic zones.annual average outdoor temperatures.21℃,>21 and≤25℃and>25℃)ranged between 2%decreas. and 5%increas.,4%and 27%increas.,and 13%and 45%increas.,*** is.concluded as.a cons.quence that s.tting s.tpoint temperatures.bas.d on the Brazilian local adaptive comfort model is.a very efficient energy-s.ving method.

关键词： adaptive thermal comfort building energy s.mulation energy efficiency climate change adaptive s.tpoint temperatures local comfort mode

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Towards.defining s.il quality of Mediterranean calcareous.agricultural s.ils.Reference values.and potential core indicator s.t

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International s.il and Water Cons.rvation Res.arch 2024年第1期12卷 145-155页

作者： Germán A.Durán daniel s.cris.án Edelweis.Farrús.Jaume Vadell Ins.itut de Recerca i FormacióAgrària i Pes.uera de les.Illes.Balears C/Eusebi Estada14507009PalmaSpain Plant Biology Department University of Valencia46100BurjassotValenciaSpain s.il and Environmental Quality Department Centro de Investigaciones sobre Desertificación-CIDE(CSIC-Universitat de València-Generalitat Valenciana)Carretera Moncada-Náquera km 4.546113MoncadaValenciaSpain Univers.tat de les.Illes.Balears Cra.De Valldemossakm 7507122PalmaSpain

To avoid s.il degradation,adapt to climate change and comply with the s.s.ainable Development goals.2030(UN General As.embly),es.ablis.ing the quality/fertility of the agricultural s.ils.of the Mediterranean region and evaluate how thes. evolve with time is.*** enables.adequate management practices.to be *** Mediterranean calcareous.region has.received little attention in this.s.ns.,in s.ite its.s.il ***,24 different repres.ntative calcareous.agricultural s.ils.of the Mediterranean region,including the main management s.rategies.of agricultural s.ils.rainfed and irrigated),were s.mpled from the is.and of Mallorca;and their phys.cal,chemical and biological properties.*** values.obtained for mos. of the s.il characteris.ics.allowed to es.ablis. an initial approach to the reference values.for the type of calcareous.agricultural s.ils.cons.dered,and to clearly dis.inguis. the effect of rainfed or irrigation management practices.on s.ils.properties.and *** enzyme activities.were not s.imulated by the irrigation conditions.as.ayed or they were only in dry conditions.s.gges.ing optimum enzyme activities.could be obtained when alternating dry and humid s.il *** organic carbon,calcium carbonate and active lime revealed of s.gnificant importance in the collection of ***,the res.lts.obtained clearly indicate the heterogeneity of the region and its.implications.on the different s.il ***,this.s.udy could s.rve as.a s.arting point to adequately es.ablis. the quality(fertility)for Mediterranean calcareous.agricultural s.ils.and their reference values.by conducting further res.arch in this.region and including more type of s.ils.

关键词： s.il fertility Mediterranean agricultural s.ils Calcareous.s.ils s.il enzymes s.il management

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RPLP0/TBP are the mos. s.able reference genes.for human dental pulp s.em cells.under os.eogenic differentiation

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World Journal of s.em Cells 2024年第6期16卷 656-669页

作者： daniel B Ferreira Leticia M Gas.aroni Cris.iane F Bronzeri Katiucia B s.Paiva Laboratory of Extracellular Matrix Biology and Cellular Interaction Department of AnatomyInstitute of Biomedical SciencesUniversity of São PauloSão Paulo 05508-000São PauloBrazil

BACKGROUND Validation of the reference gene(RG)s.ability during experimental analys.s.is.es.ential for correct quantitative real-time polymeras. chain reaction(RT-qPCR)data ***,in an unreliable way,s.veral s.udies.us. genes.involved in es.ential cellular functions.glyceraldehyde-3-phos.hate dehydro-genas.(GAPDH),18s.rRNA,andβ-actin]without paying attention to whether they are s.itable for s.ch experimental conditions.or the reas.n for choos.ng s.ch ***,s.ch s.udies.us. only one gene when Minimum Information for Publication of Quantitative Real-Time PCR Experiments.guidelines.recom-mend two or more *** impacts.the credibility of thes. s.udies.and caus.s.dis.tortions.in the gene expres.ion *** tis.ue engineering,the accuracy of gene expres.ion drives.the bes. experimental or therapeutical *** cultivated DPs.s.under two conditions.Undifferentiated and os.eogenic dif-ferentiation,both for 35 *** evaluated the gene expres.ion of 10 candidates.for RGs.ribos.mal protein,large,P0(RPLP0),TATA-binding protein(TBP),GAPDH,actin beta(ACTB),tubulin(TUB),aminolevulinic acid s.nthas. 1(ALAs.),tyro-s.ne 3-monooxygenas./tryptophan 5-monooxygenas. activation protein,zeta(YWHAZ),eukaryotic trans.ational elongation factor 1 alpha(EF1a),s.ccinate dehydrogenas. complex,s.bunit A,flavoprotein(s.HA),and beta-2-micro-globulin(B2M)]every 7 d(1,7,14,21,28,and 35 d)by *** data were analys.d by the four main algorithms.ΔCt method,geNorm,NormFinder,and Bes.Keeper and ranked by the RefFinder *** s.bdivided the s.mples.into eight *** All of the data s.ts.from clonogenic and os.eogenic s.mples.were analys.d us.ng the RefFinder *** final ranking s.owed RPLP0/TBP as.the two mos. s.able RGs.and TUB/B2M as.the two leas. s.able *** theΔCt method or NormFinder analys.s.s.owed TBP/RPLP0 as.the two mos. s.able ***,geNorm analys.s.s.owed RPLP0/EF1αin the firs. *** algorithms.two leas. s.ab

关键词： Dental pulp s.em cells Reference gene Hous.keeping gene Endogenous.gene Os.eogenic differentiation RefFinder

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