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Commanding the Commander:structure of a key protein machinery in endosomal trafficking

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Signal Transduction and Targeted Therapy 2023年第9期8卷 3877-3879页

作者： Xin Yong Chunzhuang Zhou daniel d.billadeau da Jia Key Laboratory of Birth defects and Related diseases of Women and Children Department of PaediatricsWest China Second University HospitalState Key Laboratory of BiotherapySichuan UniversityChengdu 610041China division of Oncology Research and Schulze Center for Novel Therapeutics Mayo ClinicRochesterMN 55905USA

In a recent study published in Cell1 and two bioRxiv pre-prints,2,3 three studies investigate the structure of the endosomal Commander complex through a combination of X-ray crystal-lography,cryogenic-electron microscopy(Cryo-EM),Alphafold predictions and extensive site-directed mutagenesis(Fig.1a),which sheds lights on the molecular characteristics of this evolutionarily-conserved protein machinery,and enables the mapping of mutations causing X-linked intellectual disability(XILd)and Ritscher-Schinzel syndrome(RSS).When proteins enter the endosomal network,they are either transported to lysosome for degradation,or recycled to the plasma membrane or the trans-Golgi network for *** protein machineries are essential for the process,including Retromer,Commander,and several members of the sorting nexin(SNX)*** genes are highly conserved in metazoa and can be found in almost all tissues and cells.

关键词： Commander Command sorting

NKG7 mRNA therapy increases the anti-tumor cytotoxicity of Cd8+ T cells and improves response to immune checkpoint inhibitors

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NKG7 mRNA therapy increases the anti-tumor cytotoxicity of C...

Crispr/Cas9核酸酶系统在体外培养细胞的基因敲除及无痕编辑中的应用

2021年中国肿瘤标志物学术大会暨第十五届肿瘤标志物青年科学家论坛

作者： Ti Wen Whitney Barham Ying Li Henan Zhang Joanina K.Gicobi Jacob B.Hirdler Xin Liu Hyoungjun Ham Fabrice Lucien daniel d.billadeau Haidong dong The First Hospital of China Medical University Mayo Clinic Mayo Clinic College of Medicine and Science

Translating information gained from single cell RNA-sequencing(scRNA-seq) analyses into clinical therapeutics remains a challenge. However, with the advent of technology that makes mRNA-based modulation of primary cells possible, targets identified in scRNA-seq datasets can now be manipulated directly. Here we model this approach in the context of cancer immunotherapy. We performed scRNA-seq analysis of peripheral Cd8 T cells from patients who received anti-Pd-1 checkpoint blockade and found that natural killer cell granule protein-7(NKG7) gene expression was decreased in non-responding patients. Functional assays revealed that reduced NKG7 levels compromised cytolytic granule localization and release. Furthermore, transfection of Cd8 T cells with NKG7 mRNA promoted tumor cell killing both in vitro and in vivo. Thus, we identify NKG7 as critical for response to anti-Pd-1 treatment, demonstrate the efficacy of NKG7 mRNA as a therapeutic strategy for improving cancer immunotherapy, and provide a model for translating scRNA-seq findings into mRNA-based therapy.

关键词： cancer immunotherapy anti-Pd-1 treatment scRNA-seq NKG7

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Crispr/Cas9核酸酶系统在体外培养细胞的基因敲除及无痕编辑中的...

Polyhydroxyurethanes from Biobased Monomers and CO_(2):A Bridge between Sustainable Chemistry and CO_(2) Utilization

中国生物工程学会2014年学术年会暨全国生物技术大会

作者：张金三邓志会谭晓华 daniel d.billadeau 李校堃温州医科大学药学院

作为研究基因功能的重要手段,基因的定点修饰、编辑技术一直为现代分子生物学的研究热点。近十年来基于靶向核酸酶(targeted nucleases) 的基因组编辑技术取得了里程碑式的突破与进展。锌指核酸内切酶(zinc finger endonuclease,ZFN)、... 详细信息

作为研究基因功能的重要手段,基因的定点修饰、编辑技术一直为现代分子生物学的研究热点。近十年来基于靶向核酸酶(targeted nucleases) 的基因组编辑技术取得了里程碑式的突破与进展。锌指核酸内切酶(zinc finger endonuclease,ZFN)、类转录激活因子效应物核酸酶 (transcription activator-like effector nuclease,TALEN) 及基于细菌的一种获得性免疫系统改造而成的核酸酶系统(Clustered regularly interspaced short palindromic repeats, CRISPR/CRISPR-associated,Cas9)等相关技术的应用已可对包括哺乳动物在内的几乎所有种属,细胞种类及任何基因进行编辑、修饰,并使人类遗传性疾病等的治疗成为可能。

关键词：核酸酶系统体外培养细胞基因敲除基因编辑

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Chinese Journal of Chemistry 2024年第6期42卷 652-685页

作者： Tharinee Theerathanagorn Tanika Kessaratikoon Hafeez Ur Rehman Valerio d'Elia daniel Crespy VISTEC Advanced Laboratory for Environment-Related Inorganic and Organic Syntheses Department of Materials Science and EngineeringVidyasirimedhi Institute of Science and Technology21210Wang ChanRayongThailand department of Materials Science and Engineering Vidyasirimedhi Institute of Science and Technology21210Wang ChanRayongThailand

Polyhydroxyurethanes(PHUs)have received considerable attention in the last decade as potential alternatives to traditional phosgene-based polyurethanes(PUs).The development of suitable 5CC(five membered-ring cyclic carbonate)precursors bearing multiple carbonate moieties(multi-5CCs)is a key requisite for preparing PHUs by polyaddition reaction with bis-or *** sustainable PHUs from CO_(2)-based five-membered cyclic carbonates(5CCs)obtained from biobased epoxides is a valuable strategy to bridge CO_(2) utilization and the upcycling of renewable *** this context,while many multi-5CC monomers reported in the literature are oil-based,recent efforts have led to the development of a large variety of multifunctional 5CCs that are produced by the combination of CO_(2) and renewable resources such as fatty acids and vegetable oils,lignin,terpenes,and *** this work,recent crucial advances(2019—2023)on PHUs prepared from bis-and multi-5CCs produced from CO_(2) and(partially/potentially)biobased substrates are reviewed with respect to their synthesis,thermal and mechanical properties,and their recent,emerging applications.

关键词： CO_(2) conversion Polyhydroxyurethanes Cyclic carbonates Biobased compounds Fatty acids Functional polymers Monomers Renewable resources

Charting the course to solid-state dual-ion batteries

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Carbon Energy 2024年第3期6卷 132-177页

作者： Habtom d.Asfaw Antonia Kotronia Nuria Garcia-Araez Kristina Edström daniel Brandell department of Chemistry Ångström LaboratoryUppsala UniversityUppsalaSweden department of Chemistry University of SouthamptonSouthamptonUK

An electrolyte destined for use in a dual-ion battery(dIB)must be stable at the inherently high potential required for anion intercalation in the graphite electrode,while also protecting the Al current collector from anodic dissolution.A higher salt concentration is needed in the electrolyte,in comparison to typical battery electrolytes,to maximize energy density,while ensuring acceptable ionic conductivity and operational *** recent years,studies have demonstrated that highly concentrated organic electrolytes,ionic liquids,gel polymer electrolytes(GPEs),ionogels,and water-in-salt electrolytes can potentially be used in *** can help reduce the use of solvents and thus lead to a substantial change in the Coulombic efficiency,energy density,and long-term cycle life of ***,GPEs are suited to manufacture compact dIB designs without separators by virtue of their mechanical strength and electrical *** this review,we highlight the latest advances in the application of different electrolytes in dIBs,with particular emphasis on GPEs.

关键词： anion intercalation concentrated electrolytes dual-ion battery graphite ionic liquids polymer electrolyte

Coffee Leaf Rust(Hemileia vastatrix)disease in Coffee Plants and Perspectives by the disease Control

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Phyton-International Journal of Experimental Botany 2024年第5期93卷 923-949页

作者： Alexis Salazar-Navarro Victor Ruiz-Valdiviezo Jose Joya-dávila daniel Gonzalez-Mendoza Instituto de Ciencias Agrícolas de la Universidad Autónoma de Baja California(ICA-UABC) Nuevo LeónBaja California21705México Laboratorio de Biología Molecular del Instituto Tecnológico Nacional de Tuxtla Gutierrez(TecNM) Tuxtla GutierrezChiapas29050México Facultad de Ciencias Agronómicas Campus V.Universidad Autónoma de Chiapas(UNACH) VillafloresChiapas30470México

Coffee Leaf Rust(CLR)is caused by Hemileia vastatrix in Coffea *** is one of the most dangerous phytopathogens for coffee plantations in terms of coffee productivity and coffee cup *** this review,we resume the problem of CLR in Mexico and the pathogenesis of *** review abord plant-pathogen interactions which lead a compatible or incompatible interactions and result in CLR disease or resistance,*** review abord Coffea *** response pathways involved in *** ***,current measures to control *** proliferation and germination were aborded focused on phytosanitary actions,and biological and chemical ***,new trendlines to reduce the impact of CLR as nanoparticles and nanotechnology were analyzed.

关键词： Coffee leaf rust Coffea arabica pathogenesis nanoparticles biological control

determining minimum sample size for the conditioned Latin hypercube sampling algorithm

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Pedosphere 2024年第3期34卷 530-539页

作者： daniel d.SAURETTE Asim BISWAS Richard J.HECK Adam W.GILLESPIE Aaron A.BERG School of Environmental Sciences University of Guelph50 Stone Rd EastGuelph ON N1G 2W1(Canada) Ontario Ministry of Agriculture Food and Rural Affairs1 Stone Rd WestGuelph ON N1G 2Y4(Canada) Geography Environment&GeomaticsUniversity of Guelph50 Stone Rd EastGuelph ON N1G 2W1(Canada)

In digital soil mapping(dSM),a fundamental assumption is that the spatial variability of the target variable can be explained by the predictors or environmental *** to adequately sample the predictors have been well documented,with the conditioned Latin hypercube sampling(cLHS)algorithm receiving the most attention in the dSM *** advances in sampling design,a critical gap remains in determining the number of samples required for dSM *** propose a simple workflow and function coded in R language to determine the minimum sample size for the cLHS algorithm based on histograms of the predictor variables using the Freedman-diaconis rule for determining optimal bin *** preprocessing was included to correct for multimodal and non-normally distributed data,as these can affect sample size determination from the *** on a user-selected quantile range(QR)for the sample plan,the densities of the histogram bins at the upper and lower bounds of the QR were used as a scaling factor to determine minimum sample *** technique was applied to a field-scale set of environmental covariates for a well-sampled agricultural study site near Guelph,Ontario,Canada,and tested across a range of *** results showed increasing minimum sample size with an increase in the QR *** sample size increased from 44 to 83 when the QR increased from 50% to 95% and then increased exponentially to 194 for the 99%*** technique provides an estimate of minimum sample size that can be used as an input to the cLHS algorithm.

关键词： bin width digital soil mapping normal distribution quantile range sampling design

Beyond the code:the role of histone methylation in cancer resistance and therapy

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Signal Transduction and Targeted Therapy 2024年第7期9卷 2837-2839页

作者： daniel Noerenberg Frederik damm Charité–Universitätsmedizin Berlin corporate member of Freie Universität BerlinHumboldt Universität zu Berlinand Berlin Institute of HealthDepartment of HematologyOncologyand Cancer ImmunologyBerlinGermany German Cancer Consortium(deutsches Konsortium für Translationale Krebsforschung DKTK)Partner SiteBerlinGermany

In a recently published paper in Nature,Yamagishi et *** a comprehensive exploration on the mechanistic basis and subsequent emergence of resistance of targeting histone H3 lysine trimethylation(H3K27me3)using the EZH... 详细信息

关键词： EZH2 resistance

Plant-microbial fuel cell for electrical generation through living plants: an internal resistance insight into the plant species used

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Clean Energy 2024年第5期8卷 45-53页

作者： Raymond daniel Rodriguez Martinez department of Industrial Production Faculty of EngineeringUniversidad Metropolitana1073 CaracasVenezuela

Climate change is a global threat,the presence of which has encouraged the development and implementation of renewable energies,including plant–microbial fuel cells,which could generate 6629568–33147840 MWh per year due to their large-scale *** of the main challenges associated with microbial cells is internal resistance—a parameter whose magnitude is influenced by sev-eral *** the case of plant–microbial fuel cells,adding a plant positively affects the mitigation of internal ***,the species employed is expected to play an important *** the present study,the objective was to determine as a general reference the internal resistance of cells using various plant species,among which were tomato(Solanum lycopersicum),black beans(Phaseolus vulgaris),aloe vera(Aloe vera),corn(Zea mays),and moss(dicranidae).For this purpose,an experimental procedure was carried out to compare the manual voltage measurements with a free cell concerning the voltage obtained by adding an external resistor in *** internal resistance values were determined as a function of the measured voltage,obtaining 177,179,175,324,and 233 kΩfor aloe vera,corn,tomato,black beans,and moss,*** this,it was shown that the roots of the plants represent an essen-tial addition to the internal resistance of the cell in the short *** contrast,plants with a C4 photosynthetic metabolism are more favourable,while C3 plants can also benefit from internal resistance during a more extended period of rhizodeposition.

关键词： climate change energy microorganism renewable source voltage