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Drug target discovery by magnetic nanoparticles coupled mass spectrometry

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Journal of Pharmaceutical Analysis 2021年第1期11卷 122-127页

作者： dandan xia Baoling Liu xiaowei Xu Ya Ding Qiuling Zheng Department of Pharmaceutical Analysis School of PharmacyChina Pharmaceutical UniversityNanjing210009China State Key Laboratory of Natural Medicines China Pharmaceutical UniversityNanjing210009China Key Laboratory of Drug Metabolism and Pharmacokinetics China Pharmaceutical UniversityNanjing210009China

Drug target discovery is the basis of drug *** elucidates the cause of disease and the mechanism of drug action,which is the essential of drug *** discovery performed in biological systems is complicated as proteins are in low abundance and endogenous compounds may interfere with drug ***,methods to track drug-target interactions in biological matrices are urgently *** this work,a Fe_(3)O_(4) nanoparticle-based approach was developed for drug-target screening in biofluids.A known ligand-protein complex was selected as a principle-to-proof example to validate the *** incubation in cell lysates,ligand-modified Fe_(3)O_(4) nanoparticles bound to the target protein and formed complexes that were separated from the lysates by a magnet for further *** large surface-to-volume ratio of the nanoparticles provides more active sites for the modification of chemical *** enhances the opportunity for ligand-protein interactions,which is beneficial for capturing target proteins,especially for those with low ***,a one-step magnetic separation simplifies the pre-processing of ligand-protein complexes,so it effectively reduces the endogenous ***,the present nanoparticle-based approach has the potential to be used for drug target screening in biological systems.

关键词： Drug-target discovery Nanoparticle Mass spectrometry

Magnesium alloys in tumor treatment:Current research status, challenges and future prospects

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Journal of Magnesium and Alloys 2023年第10期11卷 3399-3426页

作者： Yuchien Hsu Yupu Lu Siyi Wang Yufeng Zheng dandan xia Yunsong Liu Department of Prosthodontics Peking University School and Hospital of StomatologyBeijing100081China National Center for Stomatology National Clinical Research Center for Oral DiseasesNational Engineering Research Center of Oral Biomaterials and Digital Medical DevicesBeijing Key Laboratory of Digital StomatologyResearch Center of Engineering and Technology for Computerized Dentistry Ministry of HealthNMPA Key Laboratory for Dental MaterialsBeijing100081China Department of Dental Materials Peking University School and Hospital of StomatologyBeijing100081China Department of Prosthodontics Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineCollege of StomatologyShanghai Jiao Tong UniversityNational Center for StomatologyNational Clinical Research Center for Oral DiseasesShanghai Key Laboratory of StomatologyShanghai Research Institute of StomatologyChina Department of Materials Science and Engineering College of EngineeringPeking UniversityBeijing 100871China

Cancer is a major threat to human life worldwide. Traditional cancer treatments, such as chemotherapy and surgery, have major limitations and can cause irreversible damage to normal tissues while killing the cancer cells. Magnesium(Mg) alloys are widely reported novel potential biomedical materials with acceptable mechanical properties and good osteogenic and angiogenic properties. In this review, we summarize the Mg alloys for antitumor applications, including pure Mg and Mg alloys(Mg-Ag, Mg-Gd, Mg-Li-Zn, Mg-Ca-Sr-Zn, et al.) fabricated by casting and extruding, selective laser melting methods. Mg alloys can exhibit antitumor effect on bone tumor, breast cancer, and liver tumor,etal. What's more, after tumor tissue is eliminated, Mg alloys prevent tumor recurrence, fill tissue defects and promote tissue *** antitumor effects of Mg alloys are mainly due to their degradation products. Overall, Mg alloys show great potential in tumor treatments due to the dual function of antitumor and tissue regeneration.

关键词： Antitumor Cancer Magnesium alloys Mechanism Tissue regeneration

Research status of biodegradable metals designed for oral and maxillofacial applications:A review

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Bioactive Materials 2021年第11期6卷 4186-4208页

作者： dandan xia Fan Yang Yufeng Zheng Yunsong Liu Yongsheng Zhou Department of Prosthodontics Peking University School and Hospital of StomatologyBeijing100081China National Engineering Laboratory for Digital and Material Technology of Stomatology National Clinical Research Center for Oral DiseasesBeijing Key Laboratory of Digital StomatologyNational Medical Products AdministrationKey Laboratory for Dental MaterialsResearch Center of Engineering and Technology for Digital DentistryMinistry of HealthBeijing100081China Department of Materials Science and Engineering College of EngineeringPeking UniversityBeijing100871China

The oral and maxillofacial regions have complex anatomical structures and different tissue types,which have vital health and aesthetic *** metals(BMs)is a promising bioactive materials to treat oral and maxillofacial *** review summarizes the research status and future research directions of BMs for oral and maxillofacial ***-based BMs and Zn-based BMs for bone fracture fixation systems,and guided bone regeneration(GBR)membranes,are discussed in ***-based BMs with a moderate degradation rate and superior mechanical properties for GBR membranes show great potential for clinical ***-based BMs have a relatively low degradation rate and insoluble degradation products,which greatly limit their application and clinical ***,we proposed potential future research directions for BMs in the oral and maxillofacial regions,including 3D printed BM bone scaffolds,surface modification for BMs GBR membranes,and BMs containing hydrogels for cartilage regeneration,soft tissue regeneration,and nerve *** together,the progress made in the development of BMs in oral and maxillofacial regions has laid a foundation for further clinical translation.

关键词： Biodegradable metals Oral and maxillofacial region Bone fracture Bone fixation Bone defect Guided bone regeneration

Hydrogen selenide,a vital metabolite of sodium selenite,uncouples the sulfilimine bond and promotes the reversal of liver fibrosis

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Science China(Life Sciences) 2021年第3期64卷 443-451页

作者： Dongrui Luan Zengteng Zhao dandan xia Qiuling Zheng xiaonan Gao Kehua Xu Bo Tang College of Chemistry Chemical Engineering and Materials ScienceCollaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of ShandongKey Laboratory of Molecular and Nano ProbesMinistry of EducationInstitute of Molecular and Nano ScienceShandong Normal UniversityJinan 250014China Department of Pharmaceutical Analysis School of PharmacyState Key Laboratory of Natural MedicinesChina Pharmaceutical UniversityNanjing 210009China

Sodium selenite has alleviating effects on liver fibrosis;however,its therapeutic molecular mechanism remains ***,hydrogen selenide,a major metabolite of Na_(2)SeO_(3),was tested to uncouple the sulfilimine bond in collagen IV,the biomarker of liver fibrosis.A mouse model of liver fibrosis was constructed via a CCl_(4)-induced method,followed by the administration of 0.2 mg kg−1 Na_(2)SeO_(3)via gavage three times per week for 4 *** in H2Se,NADPH,and H_(2)O_(2)levels were monitored in real time by using NIR-H2Se,DCI-MQ-NADPH,and H_(2)O_(2)probes in vivo,respectively.H_(2)Se continuously accumulated in the liver throughout the Na_(2)SeO_(3)treatment period,but the levels of NADPH and H_(2)O_(2)*** expression of collagen IV was analyzed through Western blot and liquid chromatography-mass *** confirmed that the sulfilimine bond of collagen IV in the fibrotic mouse livers could be broken by H2Se with the Na_(2)SeO_(3)***,the therapeutic effect of Na_(2)SeO_(3)on liver fibrosis could be mainly attributed to H_(2)Se that uncoupled the sulfilimine bond to induce collagen IV *** study provided a reasonable explanation for the molecular mechanism of the in vivo function of Na_(2)SeO_(3)and the prevention of liver fibrosis by administering inorganic selenium.

关键词： hydrogen selenide sodium selenite sulfilimine bond liver fibrosis reversal collagen IV

Plasma spray of biofunctional(Mg, Sr)-substituted hydroxyapatite coatings for titanium alloy implants

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Journal of Materials Science & Technology 2019年第5期35卷 719-726页

作者： Lei Cao Ihsan Ullah Na Li Shiyu Niu Rujie Sun dandan xia Rui Yang Xing Zhang Shenyang National Laboratory for Materials Science Institute of Metal Research Chinese Academy of Sciences Shenyang 110016 China University of the Chinese Academy of Sciences Shijingshan District Beijing 100049 China School of Materials Science and Engineering University of Science and Technology of China. Hefei 239056 China Shenyang Pharmaceutical University Shenyang 110016 China University of Utah Salt Lake City UT 84112 USA

Plasma-sprayed hydroxyapatite (HA) coatings have been widely utilized in load-bearing titanium alloy implants. In this study, Mg, Sr co-substituted HA ((Mg,Sr)-HA) nano-scale powders have been synthesized, which are further used to prepare (Mg,Sr)-HA coatings on Ti-6A1-4V alloys in order to improve the biological functions. The average size of (Mg,Sr)-HA nano particles is ~75nm. The average bonding strength for (Mg,Sr)-HA coating and samples after heat treatment at 500℃ or 600℃ for 3h are 26.17±2.11 MPa, 36.07±4.48 MPa and 37.07 ±2.95 MPa, respectively. There is a significantly increase of bonding strength likely due to low residual stress after heated ***3T3-E1 cells show a high proliferation rate when cultured with (Mg,Sr)-HA coating extract compared to the normal culture medium, which also exhibit large extension and depositi on of extracellular matrices when adhered on the coating surfaces. Thus, these (Mg,Sr)-HA coatings show high bonding strength and improved biological functions, which offer promising future applications in the fields of orthopedics and dentistry.

关键词： Plasma spray Hydroxyapatite Titanium alloys Biological functions Bon ding strength

Biomimetic AgNPs@antimicrobial peptide/silk fibroin coating for infection-trigger antibacterial capability and enhanced osseointegration

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Bioactive Materials 2023年第2期20卷 64-80页

作者： Wenhao Zhou Tian Bai Lan Wang Yan Cheng dandan xia Sen Yu Yufeng Zheng Shaanxi Key Laboratory of Biomedical Metallic Materials Northwest Institute for Non-ferrous Metal ResearchXi’an710016China Department of Dental Materials Peking University School and Hospital of StomatologyBeijing100081China School of Materials Science and Engineering Peking UniversityBeijing100871China

Endowing implant surfaces with combined antibacterial and osteogenic properties by drug-loaded coatings has made great strides,but how to achieve the combined excellence of infection-triggered bactericidal and in vivo-proven osteogenic activities without causing bacterial resistance still remains a formidable ***,antimicrobial peptides(AMPs)with osteogenic fragments were designed and complexed on the surface of silver nanoparticle(AgNP)through hydrogen bonding,and the collagen structure-bionic silk fibroin(SF)was applied to carry AgNPs@AMPs to achieve infection-triggered antibacterial and *** verified by TEM,AMPs contributed to the dispersion and size-regulation of AgNPs,with a particle size of about 20 nm,and a clear protein corona structure was observed on the particle *** release curve of silver ion displayed that the SF-based coating owned sensitive pH-responsive *** the antibacterial test against *** for up to 21 days,the antibacterial rate had always remained above 99%.Meanwhile,the underlying mechanism was revealed,originating from the destruction of the bacterial cell membranes and ROS *** SF-based coating was conducive to the adhesion,diffusion,and proliferation of bone marrow stem cells(BMSCs)on the surface,and promoted the expression of osteogenic genes and collagen *** in vivo implantation results showed that compared with the untreated Ti implants,SF-based coating enhanced osseointegration at week 4 and ***,the AgNPs@AMPs-loaded SF-based coating presented the ability to synergistically inhibit bacteria and promote osseointegration,possessing tremendous potential application prospects in bone defects and related-infection treatments.

关键词： Antimicrobial peptides AgNPs Antibacterial Osteointegration Titanium

Systematic in vitro and in vivo study on biodegradable binary Zn-0.2 at%Rare Earth alloys(Zn-RE:Sc,Y,La-Nd,Sm-Lu)

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Bioactive Materials 2023年第6期24卷 507-523页

作者： Shaokang Du Yunong Shen Yufeng Zheng Yan Cheng xiaoxue Xu Dafu Chen dandan xia Academy for Advanced Interdisciplinary Studies Peking UniversityBeijing100871China School of Materials Science and Engineering Peking UniversityBeijing100871China School of Biomedical Engineering Faculty of Engineering and Information TechnologyUniversity of Technology SydneyNSW2007Australia Laboratory of Bone Tissue Engineering Beijing Laboratory of Biomedical MaterialsBeijing Research Institute of Traumatology and OrthopaedicsBeijing Jishuitan HospitalBeijing100035China Department of Dental Materials Peking University School and Hospital of Stomatology&National Center of Stomatology&National Clinical Research Center for Oral Diseases&National Engineering Research Center of Oral Biomaterials and Digital Medical Devices&Beijing Key Laboratory of Digital Stomatology&Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health&NMPA Key Laboratory for Dental MaterialsBeijing 100081China

Biomedical implants and devices for tissue engineering in clinics,mainly made of polymers and stiff metallic materials,require possibly secondary surgery or life-long *** metals for biomedical implants and devices exhibit huge potential to improve the prognosis of *** this work,we developed a new type of biodegradable binary zinc(Zn)alloys with 16 rare earth elements(REEs)including Sc,Y,La to Nd,and Sm to Lu,*** effects of REEs on the alloy microstructure,mechanical properties,corrosion behavior and in vitro and in vivo biocompatibility of Zn were systematically investigated using pure Zn as *** Zn-RE alloys generally exhibited improved mechanical properties,and biocompatibilities compared to pure Zn,especially the tensile strength and ductility of Zn-RE alloys were dramatically *** the Zn-RE alloys,different REEs presented enhancement effects at varied extent.Y,Ho and Lu were the three elements displaying greatest improvements in majority of alloys properties,while Eu,Gd and Dy exhibited least ***,the Zn-RE alloys were comparable with other Zn alloys and also exhibited superior properties to Mg-RE *** in vivo experiment using Zn-La,Zn-Ce,and Zn-Nd alloys as tibia bone implants in rabbit demonstrated the excellent tissue biocompatibility and much more obvious osseointegration than the pure Zn control *** work presented the significant potential of the developed Zn-RE binary alloys as novel degradable metal for biomedical implants and devices.

关键词： Biodegradable metals Zn-Rare Earth alloys Mechanical property Biodegradation behavior Biocompatibility

Advances in the detec

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Food Science and Human Wellness 2020年第1期9卷 40-44页

作者： Yingying Zhao dandan xia Panpan Ma xiaofeng Gao Wenyi Kang Jinfeng Wei National R&D Center for Edible Fungus Processing Technology Henan UniversityKaifeng 475004China Joint International Research Laboratory of Food&Medicine Resource Function Henan ProvinceKaifeng 475004China Kaifeng Key Laboratory of Functional Components in Health Food Kaifeng 475004China Minsheng College Henan UniversityKaifengHenan 475004China

Human health is threatened by foodborne illness and Staphylococcus aureus is a common foodborne pathogenic *** can cause food poisoning when we are infected,therefore,it is necessary to detect pathogenic *** virulence genes and detection methods of *** are summarized from *** detection methods are simple but need bacteria enrichment and to prolong detection *** technology has high specificity,but false positive results are easy to occur in its *** recent years,molecular biology methods have developed rapidly,and various PCR techniques have been applied to detect foodborne ***-time fluorescent quantitative PCR is more effective than ordinary PCR,while price is expensive when use it,the scope of use is *** is a flexible detection method,biosensor and flow cytometry are fast but the cost of detection is high and the price is relatively expensive.

关键词： Staphylococcus aureus Virulence genes Conventional methods Rapid detection methods

3D-printed Mg-1Ca/polycaprolactone composite scaffolds with promoted bone regeneration

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Journal of Magnesium and Alloys 2024年第3期12卷 966-979页

作者： xiao Zhao Siyi Wang Feilong Wang Yuan Zhu Ranli Gu Fan Yang Yongxiang Xu dandan xia Yunsong Liu Department of Prosthodontics Peking University School and Hospital of StomatologyBeijing 100081China National Center of Stomatology National Engineering Laboratory for Digital and Material Technology of StomatologyNational Clinical Research Center for Oral DiseasesBeijing Key Laboratory of Digital StomatologyResearch Center of Engineering and Technology for Computerized Dentistry Ministry of HealthNMPA Key Laboratory for Dental MaterialsBeijing 100081China Department of Dental Materials Peking University School and Hospital of StomatologyBeijing 100081China

In bone tissue engineering,polycaprolactone(PCL)is a promising material with good biocompatibility,but its poor degradation rate,mechanical strength,and osteogenic properties limit its *** this study,we developed an Mg-1Ca/polycaprolactone(Mg-1Ca/PCL)composite scaffolds to overcome these *** used a melt blending method to prepare Mg-1Ca/PCL composites with Mg-1Ca alloy powder mass ratios of 5,10,and 20 wt%.Porous scaffolds with controlled macro-and microstructure were printed using the fused deposition modeling *** explored the mechanical strength,biocompatibility,osteogenesis performance,and molecular mechanism of the Mg-1Ca/PCL *** 5 and 10 wt%Mg-1Ca/PCL composites were found to have good ***,they promoted the mechanical strength,proliferation,adhesion,and osteogenic differentiation of human bone marrow stem cells(hBMSCs)of pure *** vitro degradation experiments revealed that the composite material stably released Mg_(2)+ions for a long period;it formed an apatite layer on the surface of the scaffold that facilitated cell adhesion and *** tomography and histological analysis showed that both 5 and 10 wt%Mg-1Ca/PCL composite scaffolds promoted bone regeneration bone *** results indicated that the Wnt/β-catenin pathway was involved in the osteogenic ***,Mg-1Ca/PCL composite scaffolds are expected to be a promising bone regeneration material for clinical *** of significance:Bone tissue engineering scaffolds have promising applications in the regeneration of critical-sized bone ***,there remain many limitations in the materials and manufacturing methods used to fabricate *** study shows that the developed Ma-1Ca/PCL composites provides scaffolds with suitable degradation rates and enhanced boneformation ***,the fused deposition modeling method allows precise control of the macroscopic morphology and microsc

关键词： 3D printing Bone tissue engineering Magnesium Osteogenic Polycaprolactone Scaffold.