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Multiomics analyses of two Leonurus species illuminate leonurine biosynthesis and its evolution

Molecular Plant 2024年第1期17卷 158-177页

作者： Peng Li Meng-Xiao Yan Pan Liu dan-jie yang Ze-Kun He Yun Gao Yan Jiang Yu Kong Xin Zhong Sheng Wu Jun yang Hong-Xia Wang Yan-Bo Huang Le Wang Xiao-Ya Chen Yong-Hong Hu Qing Zhao Ping Xu shanghai Key Laboratory of Plant Functional Genomics and Resources Shanghai Chenshan Botanical GardenShanghai Chenshan Plant Science Research CenterChineseAcademy of SciencesShanghaiChina College of Life Sciences Shanghai Normal UniversityShanghai 200234China State Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghaiChina University of the Chinese Academy of Sciences Beijing 100049China The Research Center of Chiral Drugs Innovation Research Institute of Traditional Chinese MedicineShanghai University of Traditional Chinese Medicine1200 Cailun RoadShanghai 201203China College of Chemistry and Chemical Engineering Shanghai University of Engineering ScienceShanghai 201620China

The Lamiaceae family is renowned for its terpenoid-based medicinal components,but Leonurus,which has traditional medicinal uses,stands out for its alkaloid-rich ***,the principal active compound found in Leonurus,has demonstrated promising effects in reducing blood lipids and treating ***,the biosynthetic pathway of leonurine remains largely ***,we present the chromosome-level genome sequence assemblies of Leonurus japonicus,known for its high leonurine production,and Leonurus sibiricus,characterized by very limited leonurine *** integrating genomics,RNA sequencing,metabolomics,and enzyme activity assay data,we constructed the leonurine biosynthesis pathway and identified the arginine decarboxylase(ADC),uridine diphosphate glucosyltransferase(UGT),and serine carboxypeptidase-like(SCPL)acyltransferase enzymes that catalyze key reactions in this *** analyses revealed that the UGT–SCPL gene cluster evolved by gene duplication in the ancestor of Leonurus and neofunctionalization of SCPL in ***,which contributed to the accumulation of leonurine specifically in ***,our comprehensive study illuminates leonurine biosynthesis and its evolution in Leonurus.

关键词： Lamiaceae Leonurus japonicus Leonurus sibiricus genome sequence alkaloids leonurine gene cluster biosynthetic pathway evolution

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Increased excitatory amino acid transporter 2 levels in basolateral amygdala astrocytes mediate chronic stress–induced anxiety-like behavior

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Neural Regeneration Research 2025年第6期20卷 1721-1734页

作者： Xirong Xu Shoumin Xuan Shuai Chen dan Liu Qian Xiao jie Tu Shenzhen Key Laboratory of Neuroimmunomodulation for Neurological Diseases Shenzhen-Hong Kong Institute of Brain ScienceShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina CAS Key Laboratory of Brain Connectome and Manipulation Brain Cognition and Brain Disease Institute(BCBDI)Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina Guangdong Provincial Key Laboratory of Brain Connectome and Behavior the Brain Cognition and Brain Disease Institute(BCBDI)Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina University of Chinese of Academy of Sciences BeijingChina Faculty of Life and Health Sciences Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina

The conventional perception of astrocytes as mere supportive cells within the brain has recently been called into question by empirical evidence, which has revealed their active involvement in regulating brain function and encoding behaviors associated with ***, astrocytes in the basolateral amygdala have been found to play a role in the modulation of anxiety-like behaviors triggered by chronic stress. Nevertheless, the precise molecular mechanisms by which basolateral amygdala astrocytes regulate chronic stress–induced anxiety-like behaviors remain to be fully elucidated. In this study, we found that in a mouse model of anxiety triggered by unpredictable chronic mild stress, the expression of excitatory amino acid transporter 2 was upregulated in the basolateral amygdala. Interestingly, our findings indicate that the targeted knockdown of excitatory amino acid transporter 2 specifically within the basolateral amygdala astrocytes was able to rescue the anxiety-like behavior in mice subjected to stress. Furthermore, we found that the overexpression of excitatory amino acid transporter 2 in the basolateral amygdala, whether achieved through intracranial administration of excitatory amino acid transporter 2agonists or through injection of excitatory amino acid transporter 2-overexpressing viruses with GfaABC1D promoters, evoked anxiety-like behavior in mice. Our single-nucleus RNA sequencing analysis further confirmed that chronic stress induced an upregulation of excitatory amino acid transporter 2 specifically in astrocytes in the basolateral amygdala. Moreover, through in vivo calcium signal recordings, we found that the frequency of calcium activity in the basolateral amygdala of mice subjected to chronic stress was higher compared with normal *** knocking down the expression of excitatory amino acid transporter 2 in the basolateral amygdala, the frequency of calcium activity was not significantly increased, and anxiety-like behavior was ob

关键词： anxiety astrocytes basolateral amygdala behavior dihydrokainic acid excitatory amino acid transporter 2 fiber photometry glutamate LDN-212320 transporter

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Increased expression of human calcium-activated chloride channel 1 is correlated with mucus overproduction in the airways of Chinese patients with chronic obstructive pulmonary disease

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Chinese Medical Journal 2007年第12期120卷 1051-1057页

作者： WANG Ke FENG Yu-ling WEN Fu-qiang CHEN Xue-rong OU Xue-mei XU dan yang jie DENG Zhi-pin Division of Pulmonary Diseases State Key Laboratory of Biotherapy of China and Department of Respiratory Medicine West China Hospital of Sichuan University Chengdu 610041 China

Background Chronic obstructive pulmonary disease （COPD） is usually complicated with mucus overproduction in airway. Recently the increased expression of the human calcium-activated chloride channel 1 （CaCC1） was found to play an important role in mucus overproduction in the asthmatic airways. To investigate the relationship of CaCC1 and mucus overproduction in the airway of Chinese patients with COPD, the expressions of CaCC1, MUC5AC and mucus in bronchial tissues were examined. Methods Bronchial tissues were obtained from fiberoptic bronchoscopy and bronchial biopsy in West China Hospital from April to July in 2004. Twenty-five patients were diagnosed as the patients with COPD overproduction, and other 20 were the control subjects. The expressions of CaCC1, MUC5AC and mucin in bronchial tissues were detected by reverse transcriptase-polymerase chain reaction （RT-PCR）, in situ hybridization with digoxigenin （DIG）-Iabeled RNA probe, immunohistochemical and alcian blue-periodic acid Schiff （AB-PAS） staining, respectively. Results Compared with the control group, the stronger expressions of CaCC1 were further detected throughout the bronchial tissues from patients with COPD （P〈0.01）. Furthermore, the stronger expressions of the CaCC1 mRNA were related to the severity of airflow obstruction. Samples from COPD showed a stronger staining for MUC5AC than those in control subjects （P〈0.01） and AB-PAS staining revealed more mucins in COPD patients＇ submucosal gland comparing with that in control subjects （P〈0.01）. Expression levels of the CaCC1 mRNA were respectively negatively correlated with the patients＇ forced expiratory volume in one second （FEV~） / forced vital capacity （FVC） data, FEV1% predicted data, V50% predicted data, V25% predicted data （r=-0.43, r=-0.43, r=-0.35, r=-0.36, P〈0.01, P〈0.01, P〈0.05, P〈0.05）. While the expression levels of the CaCC1 mRNA were well correlated with the expression levels of the MUC5AC mRNA of airway epithelium and the PAS-AB staine

关键词： calcium-activated chloride channel 1 mucin 5AC mucin mucus overproduction chronic obstructive pulmonary disease

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Decreased expression of human aquaporin-5 correlated with mucus overproduction in airways of chronic obstructive pulmonary disease

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Acta Pharmacologica Sinica 2007年第8期28卷 1166-1174页

作者： Ke WANG Yu-lin FENG Fu-qiang WEN~2 Xue-rong CHEN Xue-mei OU dan XU jie yang Zhi-ping DENG Division of Pulmonary Diseases,State Key Laboratory of Biotherapy of China,and Department of Respiratory Medicine,West China Hospital of Sichuan University,Sichuan 610041,China Division of Pulmonary Diseases and Department of Respiratory Medicine State Key Laboratory of Biotherapy of China West China Hospital of Sichuan University Sichuan China

Aim:To investigate the relationship between aquaporin-5(AQP5)expression andmucus overproduction in the airways of Chinese patients with chronic obstruc-tive pulmonary disease(COPD)and the correlation with pulmonary ***:Bronchial tissues were obtained from fiberoptic bronchoscopyand bronchial biopsy in West China Hospital from April to July ***-fivepatients were diagnosed as COPD patients,and another 20 were diagnosed as thecontrol *** expressions ofAQP5,mucin 5AC(MUC5AC),and mucin inbronchial tissues were detected by semiquantitative RT-PCR,in situ hybridization,and immunohistochemical and alcian blue-periodic acid-Schiff(AB-PAS)staining,***:Compared with the control group,an attenuated expres-sion of AQP5was detected throughout the bronchial tissues from patients withCOPD(P0.05).Simultaneously,increased staining of MUC5AC and mucus in submucosal glandswere noted(P0.05).The decreased expression of AQP5mRNA was correlatedwith forced expiratory volume in 1 s(FEV1)/forced vital capacity(FVC)(r=0.60,P<0.01),FEV1% predicted value(r=0.60,P<0.01),maximal expiratory flow in 50%vital capacity(V50)% predicted value(r=0.55,P<0.01),and maximal expiratory flowin 25% vital capacity(V25)% predicted value(r=0.45,P<0.01).The decreasedexpression was negatively correlated with MUC5AC mRNA of the epitheliumairways(r=-0.45,P<0.01)and the AB-PAS-stained area of submucosal glands(r=-0.61,P<0.01).The upregulation of MUC5AC mRNA correlated with the posi-tively AB-PAS-stained area of submucosal glands and correlated negativelywith FEV1/FVC(r=-0.53;P<0.01),FEV1% predicted value(r=-0.53;P<0.01),V50%predicted value(r=-0.48;P<0.01),and V25% predicted value(r=-0.4

关键词： aquaporin-5 mucin 5AC mucin mucus overproduction chronic obstructive pulmonary disease

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Constructing 2D bismuth-based heterostructure for highly efficient photocatalytic CO2 reduction

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Tungsten 2025年第1期7卷 71-79页

作者： Ruo-Hang Xu Hao-Yu Jiang dan-dan Cui Kun-Rong Du Hong-Run Zhang Sheng-jie Xu Ya-Qi Li Zhi-Zhen Ren Liang Wang Wei-Chang Hao Yi Du School of Physics and BUAA-UOW Joint Research Centre Beihang University Shenzhen Institute of Beihang University Centre for Catalysis and Clean Energy School of Environment and Science Griffith University

Reforming carbon dioxide（CO2） into fuels and valuable chemicals using solar energy holds significant potential for emission reduction and addressing energy supply *** recombination of photocarriers remains a major obstacle,however,to enhancing solar energy conversion efficiency in the photocatalytic reaction *** two-dimensional materials,constructing heterogeneous structures has emerged as an effective strategy to enhance the separation of photoexcited carriers by leveraging the internal electric field at the heterogeneous ***,we present a facile strategy for preparing Bi4NbO8Br/Bi2WO6plate-on-plate Z-scheme junctions with a strong internal electric field by in situ *** unique heterostructure facilitates efficient separation and transport of photoexcited charges by harnessing the internal electric field induced by chemical bonding at the lateral ***,the Bi4NbO8Br/Bi2WO6heterostructure exhibits enhanced CO2photoreduction properties,along with a CO yield rate of approximately 35 μmol·g-1·h-1under full light *** work offers novel insights into the synthesis of unique hetero structures intended to enhance the efficiency of photocatalytic CO2reduction.

关键词： Bi4NbO8Br/Bi2WO6 CO2 reduction Heterostructures Photocatalysts

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新疆特色食品中优良乳酸菌的筛选及分离鉴定

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中国酿造 2017年第4期36卷 50-53页

作者：杨玉新韦鹏李丹杨婕宋振新疆中亚食品研发中心有限公司新疆乌鲁木齐830000

该研究利用传统培养法从自然发酵的新疆特色食品中分离筛选出3株菌,通过菌株形态观察、生理生化试验及16S rDNA序列分析对菌株进行鉴定。结果表明,菌株L-6、L-7和L-8分别被鉴定为肠膜明串珠菌(Leuconostoc mesenteroides)、植物乳杆菌(L... 详细信息

该研究利用传统培养法从自然发酵的新疆特色食品中分离筛选出3株菌,通过菌株形态观察、生理生化试验及16S rDNA序列分析对菌株进行鉴定。结果表明,菌株L-6、L-7和L-8分别被鉴定为肠膜明串珠菌(Leuconostoc mesenteroides)、植物乳杆菌(Lactobacillus plantarum)及短乳杆菌(Lactobacillus brevis)。产酸试验结果表明,这3株菌均具有较强的产酸能力,发酵12 h后发酵液pH值可达4.0以下,其中菌株L-7产酸能力最强,在15 h后其发酵液pH值在3.5以下,可用于辣椒酱或其他发酵食品发酵剂的研制。

关键词：新疆特色制品乳酸菌筛选鉴定

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数字学术中心对高校图书馆服务创新的启示

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新世纪图书馆 2018年第6期 71-77页

作者：孔丹杨洁王燕平西安建筑科技大学图书馆馆员陕西西安710055

数字时代开启了学术研究的新模式,为适应这种变化,数字学术中心开始出现,并且近几年在国外高校图书馆迅速发展,成为图书馆参与学术研究及实现服务创新的重要方式。成立较早、经验丰富的威斯康星大学数字收藏中心和新近成立代表中心发展... 详细信息

数字时代开启了学术研究的新模式,为适应这种变化,数字学术中心开始出现,并且近几年在国外高校图书馆迅速发展,成为图书馆参与学术研究及实现服务创新的重要方式。成立较早、经验丰富的威斯康星大学数字收藏中心和新近成立代表中心发展新趋势的杜克大学学术研究与协作边缘社区,为我国高校图书馆在空间再造、数字支持、人员结构、开放存取、数字出版等服务模式的转变方面提供了启示和借鉴。

关键词：数字学术中心高校图书馆服务模式创新

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Sandwich教学法结合案例教学法在流行病学教学中的应用

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教育教学论坛 2017年第3期 173-175页

作者：李涛李曼刘文宣杨磊曹丹丹杨洁河北医科大学公共卫生学院流行病与卫生统计学教研室河北石家庄050017

流行病学理论性较强,传统教学法的教学效果欠佳。我们将Sandwich教学法结合案例教学法运用到流行病学的理论教学中,提高了学生的学习兴趣,培养了学生的学习主动性,以及分析问题、解决问题的能力。Sandwich教学法结合案例教学法使学生更... 详细信息

流行病学理论性较强,传统教学法的教学效果欠佳。我们将Sandwich教学法结合案例教学法运用到流行病学的理论教学中,提高了学生的学习兴趣,培养了学生的学习主动性,以及分析问题、解决问题的能力。Sandwich教学法结合案例教学法使学生更容易掌握流行病学的理论知识以及实际应用,取得了较好的教学效果。

关键词： Sandwich教学法案例流行病学教学改革

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服务器无感知计算系统性能优化技术研究综述

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软件学报 2025年第1期36卷 47-78页

作者：杨光刘杰曲慕子王帅叶丹钟华中国科学院大学中国科学院软件研究所软件工程技术研究开发中心计算机科学国家重点实验室(中国科学院软件研究所)

服务器无感知计算是新兴的云计算模式,它基于“函数即服务(FaaS)”的范式,以函数为部署和调度的基本单位,为用户提供大规模并行和自动伸缩的函数执行服务,且无需用户管理底层资源.对于用户,服务器无感知计算能够帮助他们摆脱集群底层基... 详细信息

服务器无感知计算是新兴的云计算模式,它基于“函数即服务(FaaS)”的范式,以函数为部署和调度的基本单位,为用户提供大规模并行和自动伸缩的函数执行服务,且无需用户管理底层资源.对于用户,服务器无感知计算能够帮助他们摆脱集群底层基础设施管理的负担,专注于业务层的开发和创新;对于服务提供商,服务器无感知计算将应用分解为细粒度的函数,极大地提高了调度效率和资源利用率.显著的优势让服务器无感知计算迅速吸引了业界的注意,然而,服务器无感知计算与传统云计算迥然不同的计算模式以及对任务各方面的严格限制给应用的迁移带来了诸多障碍,各种越来越复杂的任务也对服务器无感知计算的性能提出了越来越高的要求,服务器无感知计算的性能优化成为一个重要的研究课题.从4个方面对服务器无感知计算系统性能优化技术的相关研究工作进行梳理和综述,并介绍现有的系统实现.(1)介绍面向典型任务的优化技术,包括任务适配和针对特定任务的系统优化;(2)综述沙箱环境的优化工作,包括沙箱方案和冷启动优化技术,它们是决定函数运行速度的核心;(3)概括I/O和通信技术的优化,它们是服务器无感知计算应用程序的主要性能瓶颈;(4)简述相关的资源调度技术,包括面向平台和面向用户的调度策略,它们决定着系统的资源利用率和任务的执行效率.最后,总结当前服务器无感知计算性能优化技术所面临的问题和挑战,并展望未来可能的发展方向.

关键词：服务器无感知计算函数即服务云函数云计算性能优化

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Cross-stacking crystal structural configuration of integrated cathode materials for proton-conducting solid oxide fuel cells

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Rare Metals 2024年第11期43卷 6088-6095页

作者： Jun-Yi Gong dan-dan yang Wei Liu jie Hou School of Resource Environment and Safety Engineering University of South ChinaHengyang 421001China CAS Key Laboratory of Materials for Energy Conversion and Collaborative Innovation Center of Suzhou Nano Science and Technology University of Science and Technology of ChinaHefei 230026China

The absence of cathode materials with excellent activity and stability has hampered the widespread application of proton-conducting solid oxide fuel cells(H-SOFCs).Sm_(2)Ba_(1.33)Ce_(0.67)Cu_(3)O_(9)(SBCC)is a layered material formed by the cross-stacking of a[CuO_(5)layer][CeO_(2)-like layer][CuO_(5)layer][Sm_(x)Ba_(1-x)CuO_(y)perovskite layer].This complex structure could potentially enhance electrocatalytic performance owing to the integration of differentstructural,chemical and electronic properties.

关键词： structural stacking solid

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