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Distinct structural characteristics define a new subfamily of Mycoplasma ferritin

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Chinese Chemical Letters 2022年第11期33卷 4952-4955页

作者： Wenming Wang Xiaojia Liu Yajie Wang Yuan Wang dan fu Hongfang Xi Yi Zhao Hongfei Wang Institute of Molecular Science Shanxi UniversityTaiyuan 030006China State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy Nankai UniversityTianjin 300350China Shanxi Key Laboratory of Pharmaceutical Biotechnology Taiyuan 030006China

Ferritins can generally be divided into four subfamilies based on their structural characteristics,namely,the classic ferritins(Ftns),bacterioferritins(Bfrs),DNA-binding proteins from starved cells(Dps’),and encapsulated ferritins(Enc Ftns).However,the ferritin from Mycoplasma penetrans(Mpef)possesses a particular ferroxidase center with an extreme low activity and exhibits unusual characteristics,indicating that it could be a member of a quite different subfamily of ***,the crystal structure of the ferritin from Ureaplasma urealyticum(Uurf)is presented,Mpef and Uurf have very similar properties,though they display very low sequence ***,ferritins from Mycoplasma with these unique properties do not belong to any known subfamily,but they should rather be placed in a novel ferritin subfamily,which we term Mycoplasma Ferritin(Mfr).

关键词： Ferritin Ferritin subfamily Ureaplasma Mycoplasma Crystal structure

Insight into the possible mechanism of the summer diapause of Delia antiqua （Diptera： Anthomyiidae） through digital gene expression analysis

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Insect Science 2016年第3期23卷 438-451页

作者： You-Jin Hao Yu-Juan Zhang Feng-Ling Si dan-Ying fu Zheng-Bo He Bin Chen Institute of Entomology and Molecular Biology College of Life Sciences Chongqing Normal University Chongqing China

The onion fly, Delia antiqua, is a major underground agricultural pest that can enter pupal diapause in the summer and winter seasons. However, little is known about its molecular regulation due to the lack of genomic resources. To gain insight into the possible mechanism of summer diapause （SD）, high-throughput RNA-Seq data were generated from non-diapause （ND） and SD （initial, maintenance and quiescence phase） pupae. Three pair-wise comparisons were performed and identified, 1380, 1471 and 435, and were significantly regulated transcripts. further analysis revealed that the enrichment of several functional terms related to juvenile hormone regulation, cell cycle, carbon hydrate and lipid metabolism, innate immune and stress responses, various signalling transductions, ubiquitin-dependent proteosome, and variation in cuticular and cytoskeleton components were found between ND and SD and between different phases of SD. Global characterization oftranscriptome profiling between SD and ND contributes to the in-depth elucidation of the molecular mechanism of SD. Our results also offer insights into the evolution of insect diapause and support the importance of using the onion fly as a model to compare the molecular regulation events of summer and winter diapauses.

关键词： Delia antiqua digital gene expression possible mechanism qPCR RNA-Seq summer diapause

Adhesion of bone marrow mesenchymal stem cells on porous titanium surfaces with strontium-doped hydroxyapatite coating

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2017年第9期18卷 778-788页

作者： dan-li fu Qiao-hong JIANG fu-ming HE Bai-ping fu Department of General Dentistry West Branch of Stomatology Hospital School of Medicine Zhejiang University Department of Oral Implantology Stomatology Hospital School of Medicine Zhejiang University Department of Prosthodontics Stomatology Hospital School of Medicine Zhejiang University

Objective: To determine the adhesion behavior of bone marrow mesenchymal stem cells(MSCs) on a titanium surface with a nanostructured strontium-doped hydroxyapatite(Sr-HA) coating. Methods: Sr-HA coatings were applied on roughened titanium surfaces using an electrochemical deposition method. Primary cultured rat MSCs were seeded onto Sr-HA-, HA-coated titanium, and roughened titanium surfaces, and they were then cultured for 1,6, and 24 h.3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay was used to determine the metabolic condition of the cells. Scanning electron microscopy(SEM) was used to observe the cell morphology. The cytoskeletal structure was analyzed using fluorescence actin staining to characterize cell adherence. Quantitative real-time reverse transcription polymerase chain reaction(RT-q PCR) was used to analyze the gene expression levels of FAK(focal adhesion kinase), vinculin, integrin β1, and integrin β3 after culturing for 24,48, and 72 h. Results: MSCs cultured on the Sr-HA surface showed better cell proliferation and viability. Improvement of cell adhesion and structural rearrangement of the cytoskeleton were observed on the Sr-HA surface. The gene expression of FAK, vinculin, integrin β1, and integrin β3 was also elevated on the Sr-HA surface. Conclusions: Cell viability, adhesion, cell morphology, and the cytoskeletal structure were all upregulated considerably by the titanium surface modified with a Sr-HA coating.

关键词： Cell adhesion Bone marrow mesenchymal stem cell Strontium-doped hydroxyapatite Titanium

Research on Mode and Tendency of Web2.0-based Online Advertising

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Research on Mode and Tendency of Web2.0-based Online Adverti...

Screening of QHF formula for effective ingredients from Chinese herbs and its anti-hepatic cell cancer effect in combination with chemotherapy

2011 International Conference on Computer Science and Information Technology(ICCSIT 2011)

作者： fu dan Liao Bingyi School of Humanities Huazhong University of Science and Technology School of Journalism and Communication Wuhan University

ISBN: (纸本)9781612848334

This paper focuses on the differences of marketing environment in the context of new network technology, and the changes of online advertising marketing mode under the different marketing environment. The authors propose the trends of online advertising in the age of Web2.0, which are the accurate marketing, viral marketing, word of mouth marketing in the internet community and database marketing of online advertising.

关键词： Web2.0 Online advertising Advertising Marketing mode E-business

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Chinese Medical Journal 2008年第4期121卷 363-368页

作者： CHEN Tao LI dan fu Ya-ling HU Wei Department of Traditional Chinese Medicine Three (Jorges University Medical College Yichang Hubei 443002 China

Background Recent studies have shown that effective ingredients of Chinese herbs are used more and more widely in the treatment or co-treatment of cancers, however, they are usually used separately and there has been limited research about joint application of Chinese herbs in multi-modal treatment. The aim of this study was to screen a QHF （Q： Qingrejiedu, H： Huoxuehuayu and F： fuzhengguben） formula for effective ingredients from Chinese medicines and assess its anti-hepatic cell cancer （HCC） effect in combination with chemotherapy.Methods Six effective ingredients from Chinese medicine were selected based on the previous literature and used in the study. The QHF formula and the best ratio of ingredients were evaluated in H22 mouse （KM） models with solid tumors and ascites tumors by uniform design and monitoring inhibition of tumor growth and survival. We then observed the anti-hepatic cell cancer （HCC） effect of QHF when combined with cisplatin （DDP） in H22 mouse （Balb/c） models with solid tumors and ascites tumors. Evaluating of the therapeutic effect included the general condition of the mice, inhibition of tumor growth, survival, changes in body weight, thymus index, spleen index and WBC counts.Results The optimal QHF dose ratio for anti-hepatic cell cancer treatment was： 800 mg/kg Cinobufotalin, 14 mg/kg Ginsenosides Rg3, 5.5 mg/kg PNS and 100 mg/kg Lentinan. Treatment was more efficient in inhibiting the growth of transplanted tumors in H22 mice when using the QHF formula （55.91%） than using Cinobufotalin （33.25%）, Ginsenosides Rg3 （35.11%）, PNS （27.12%） or Lentinan （4.97%） separately. QHF also prolonged the life of H22 ascites hepatic cancer mice more efficiently （38.13%） than Cinobufotalin （25.00%）, Ginsenosides Rg3 （27.27%）, PNS （23.30%） or Lentinan （24.43%）. QHF combined with DDP could reduce DDP-induced leucopenia, spleen and thymus atrophy and other toxic reactions. Combining QHF with DDP the tumor growth inhibition reached 82.54% with a 66.83% incr

关键词： effective ingredients hepatic cell cancer uniform design QHFformula cisplatin

An Empirical Analysis on Mental Health of Agricultural College Students

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Asian Agricultural Research 2017年第6期9卷 110-112页

作者： dan fu Ning MA Shuzhen WANG Northwest A & F University

Based on the empirical survey of 2013-2016 college students in Northwest A&F University,we analyze the 9260 valid samples,and the survey involves emotional state,self-worth,psychological adjustment capacity and students’ attitude towards mental health *** the basis of statistical data,we summarize the general situation of agricultural college students’ mental health,analyze the problems in agricultural college students’ psychological development and bring forward the feasible recommendations.

关键词： College students Mental health Current situation Recommendations

Structure of African swine fever virus p15 reveals its dual role for membrane-association and DNA binding

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Protein & Cell 2020年第8期11卷 606-612页

作者： dan fu Dongming Zhao Wei Zhang Guangshun Zhang Mingyu Li Zheng Zhang Yuhui Wang Dongdong Sun Peng Jiao Cheng Chen Yu Guo Zihe Rao State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy Nankai UniversityTianjin 300350People's Republic of China State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150001People's Republic of China School of Life Sciences Tianjin UniversityTianjin 300071People's Republic of China

Dear Editor, African swine fever (ASF) is a highly contagious disease of domestic pigs caused by African swine fever virus (ASFV),with mortality rates approaching 90%-100%.The first ASF case was described in 1921 in *** the past decades, ASF has spread from Sub-Saharan Africa to Europe and Asia, and pose a huge threat to global food *** the first ASF case reported in August 2018, more than 10 million pigs had been culled in China at an enormous economic *** risk of ASFV mutating to infect humans is extremely ***, ASF not only threatens food security but also severely strikes the world economy in many fields, such as the global supply of the blood thinner heparin,most of which is produced from ***, ASF is a strictly monitored infectious animal disease by OIE (World Organization for Animal Health), unfortunately, there is no approved drug or vaccine against this lethal viral disease.

关键词： swine fever African

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成本管理会计现状分析及发展设想

财经问题研究 2001年第4期 64-65页

作者：傅丹东北财经大学会计学院辽宁大连116025

管理会计引入我国已有 2 0年的时间了 ,虽然在实际工作中已有一些应用 ,但与经济发展和管理需要还存在着相当大的差距。本文对管理会计应用中存在的问题进行了剖析 ,提出了将成本会计与管理会计合并 ,加强成本管理会计在实际工作中的地... 详细信息

管理会计引入我国已有 2 0年的时间了 ,虽然在实际工作中已有一些应用 ,但与经济发展和管理需要还存在着相当大的差距。本文对管理会计应用中存在的问题进行了剖析 ,提出了将成本会计与管理会计合并 ,加强成本管理会计在实际工作中的地位与作用的设想。

关键词：成本管理会计地位作用管理会计

Clustering Gene Expression Data Based on Predicted Differential Effects of GV Interaction

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Genomics, Proteomics & Bioinformatics 2005年第1期3卷 36-41页

作者： Hal-Yah Pan Jun Zhu dan-fu Han Institute of Bioinformatics Zhejiang University Hangzhou 310029 China Department of Mathematics Zhejiang University Hangzhou 310027 China.

Microarray has become a popular biotechnology in biological and medical research. However, systematic and stochastic variabilities in microarray data are expected and unavoidable, resulting in the problem that the raw measurements have inherent “noise” within microarray experiments. Currently, logarithmic ratios are usually analyzed by various clustering methods directly, which may introduce bias interpretation in identifying groups of genes or samples. In this paper, a statistical method based on mixed model approaches was proposed for microarray data cluster analysis. The underlying rationale of this method is to partition the observed total gene expression level into various variations caused by different factors using an ANOVA model, and to predict the differential effects of GV （gene by variety） interaction using the adjusted unbiased prediction （AUP） method. The predicted GV interaction effects can then be used as the inputs of cluster analysis. We illustrated the application of our method with a gene expression dataset and elucidated the utility of our approach using an external validation.

关键词： gene expression clustering analysis predicting G V interaction effects