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Genome-wide and candidate gene association studies identify BnPAP17 as conferring the utilization of organic phosphorus in oilseed rape

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Journal of Integrative Agriculture 2024年第4期23卷 1134-1149页

作者： Ping Xu Hao Li Haiyuan Li Ge Zhao Shengjie dai Xiaoyu Cui Zhenning Liu Lei shi Xiaohua Wang College of Agriculture and Forestry Science Linyi UniversityLinyi 27600China National Key Laboratory of Crop Genetic Improvement/Microelement Research Centre/Key Laboratory of Arable Land Conservation(Middle and Lower Reaches of Yangtze River) Ministry of Agriculture and Rural Affairs/Huazhong Agricultural UniversityWuhan 430070China

Phosphorus(P)is essential for living plants,and P deficiency is one of the key factors limiting the yield in rapeseed production *** the most important organ for plants,root morphology traits(RMTs)play a key role in P *** investigate the genetic variability of RMT under low P availability,we dissected the genetic structure of RMTs by genome-wide association studies(GWAS),linkage mapping and candidate gene association studies(CGAS).A total of 52 suggestive loci were associated with RMTs under P stress conditions in 405 oilseed rape *** purple acid phosphatase gene BnPAP17 was found to control the lateral root number(LRN)and root dry weight(RDW)under low P *** expression of BnPAP17 was increased in shoot tissue in P-efficient cultivars compared to root tissue and P-inefficient cultivars in response to low P ***,the haplotype of BnPAP17^(Hap3)was detected for the selective breeding of P efficiency in oilseed ***-expression of the BnPAP17^(Hap3)could promote the shoot and root growth with enhanced tolerance to low P stress and organic phosphorus(Po)utilization in oilseed ***,these findings increase our understanding of the mechanisms underlying BnPAP17-mediated low P stress tolerance in oilseed rape.

关键词： genome-wide association studies(GWAS) root morphology traits(RMTs) organic phosphorus(Po) oilseed rape BnPAP17

Study on One-Dimensional Model of Traffic Flow with Stochastic Deceleration via Lattice Boltzmann Method

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Journal of Shanghai University(English Edition) 2001年第2期5卷 104-106页

作者： XUE Yu, DONG Li yun, dai shi qiang Shanghai Institute of Applied Mathematics and Mechanics, Shanghai University, Shanghai 200072, China Shanghai Institute of Applied Mathematics and Mechanics Shanghai University Shanghai China

One dimensional traffic model was studied with the consideration of stochastic deceleration by using the lattice Boltzmann method. The evolution equation for vehicle density was derived, which has the form of the Burgers equation exhibiting the effect of viscosity corresponding to the deceleration. The simulation with the model shows that the variation of vehicle density in space tends to a periodic one, which implies the existence of kinematic waves in the 1D traffic flow and coincides with theoretical prediction.

关键词： lattice Boltzmann method traffic flow Burgers equation

Maternal supplementation with mulberry-leaf flavonoids improves the development of skeletal muscle in the offspring of chickens

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Animal Nutrition 2024年第3期18卷 72-83页

作者： Zhenwu Huang Hongjian dai Simeng Li Zhe Wang Quanwei Wei Zhonghua Ning Yuming Guo Fangxiong shi Zengpeng Lv State Key Laboratory of Animal Nutrition and Feeding College of Animal Science and TechnologyChina Agricultural UniversityBeijing100193China College of Animal Science and Technology Nanjing Agricultural UniversityNanjing210095China State Key Laboratory of Animal Nutrition and Feeding Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing100193China School of Biotechnology Aksu Vocational and Technical CollegeAksu843000China

The development of skeletal muscle is a crucial factor in determining the meat yield and economic benefits of broiler *** research has shown that mulberry leaves and their extracts can be used to significantly improve the growth performance of livestock and *** present study aims to elucidate the mechanisms involved in the regulation of skeletal muscle development in broiler offspring by dietary mulberry-leaf flavonoids(MLF)supplementation from the perspective of maternal effect theory.A total of 270 Qiling broiler breeder hens were randomly assigned to 3 treatments with different doses of MLF(0,30,60 mg/kg)for 8 weeks before collecting their fertilized *** chicken offspring at 13 and 19 d of embryonic stage,and from 1 to 28 d old after hatching were included in this *** results showed that maternal supplementation increased the breast muscle weight and body weight of the offspring at the embryo and chick stages(P<0.05).This was followed by increased cross-sectional area of pectoral muscle fibres at 14 d(P<0.05).Further determination revealed a tendency towards increased serum levels of insulin-like growth factor 1(IGF-1)(P=0.092)and muscle fibre count(P=0.167)at 1 d post-hatching following maternal MLF treatment,while serum uric acid(UA)was decreased at 14 d after hatching(P<0.05).Moreover,maternal MLF supplementation significantly up-regulated the mRNA expression of the myogenic regulatory factor Myf5 in skeletal muscle at the both embryonic and growth stages(P<0.05).The relative abundance of the downstream protein of BMPR2,Smad1 and p-Smad1/5/9 in the TGFβsignalling pathway was significantly increased by maternal MLF ***,the increased expression of the target protein p-mTOR in the breast muscle of the offspring chicks is in accordance with the improved growth rate of the breast and the *** conclusion,maternal MLF supplementation can promote muscle protein metabolism and muscle fibre development of chick embryos

关键词： Broiler breeder hen Mulberry-leaf flavonoids Offspring Protein metabolism Skeletal muscle

基于项目化学习方式的博物馆教育路径探索——以福建博物院的实践为例

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中国博物馆 2023年第4期 81-86页

作者：戴石福建博物院福州350001

福建博物院近年来尝试以项目化学习方式开展博物馆教育课程。以“寻味丝路——大家来策展”教育项目为例,通过设计真实世界中具有挑战性的驱动性问题,配合适当的“支架式”教学支持,保障学生高阶思维和综合素养的提升,最后利用有效练习... 详细信息

福建博物院近年来尝试以项目化学习方式开展博物馆教育课程。以“寻味丝路——大家来策展”教育项目为例,通过设计真实世界中具有挑战性的驱动性问题,配合适当的“支架式”教学支持,保障学生高阶思维和综合素养的提升,最后利用有效练习、及时反馈和多元化评估进一步优化学生项目化学习成果。

关键词：博物馆博物馆教育 PBL项目化学习驱动性问题 “支架式”教学支持

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PLK方法与符号运算

应用数学和力学 2001年第3期22卷 221-227页

作者：戴世强上海市应用数学和力学研究所上海大学上海200072

阐述将PLK方法与符号运算相结合的途径和有效性·　首先简述PLK方法的思路和发展简史 :其次 ,概述运行符号运算时经常遇到的“中间表达式爆炸”困难 ,为克服这一困难 ,作者提出一种半逆序算法 :通过以符号形式“冻结”中间表达式中冗长... 详细信息

阐述将PLK方法与符号运算相结合的途径和有效性·　首先简述PLK方法的思路和发展简史 :其次 ,概述运行符号运算时经常遇到的“中间表达式爆炸”困难 ,为克服这一困难 ,作者提出一种半逆序算法 :通过以符号形式“冻结”中间表达式中冗长的部分 ,到最后阶段再予“解冻” ;并且通过综述作者在一系列非线性波动和非线性振动方面的工作 ,讨论PLK_符号运算方法的具体应用 ,其中 ,Duffing方程的摄动解的计算机延伸表明 ,用PLK方法导得的渐近级数解的收敛半径为 1,从而大大拓广了解的适用范围 ;分层流体中内孤立波和超弹性杆中孤立波对撞的研究表明 ,用所提出的方法可以进行手工计算难以进行的复杂运算 ,借此可得出高阶演化方程和高阶渐近解 ,正确地解释实验结果 ;并说明采用半逆序算法后 ,可在微机上实现繁复的符号运算·　最后得出结论 :借助于符号运算 ,可大大增强PLK方法的生命力 ,至少对保守系统的振动和波动问题的求解。

关键词： PLK方法摄动方法符号运算中间表达式爆炸边界层非线性KLEIN-GORDON方程

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美日欧应对关键原材料供应短缺的政策与动向

国土资源科技管理 2014年第3期31卷 113-121页

作者：时代国土资源部信息中心北京100812

综述近年美国、日本和欧盟在关键原材料方面的战略布局、资金支持、勘探开采和技术研发情况。美日欧在积极挖掘内部潜力,重启老矿山和开发新矿产地的同时,不断寻求海外矿产资源,促进原材料可持续供应,并且都启动了有关替代材料、降低消... 详细信息

综述近年美国、日本和欧盟在关键原材料方面的战略布局、资金支持、勘探开采和技术研发情况。美日欧在积极挖掘内部潜力,重启老矿山和开发新矿产地的同时,不断寻求海外矿产资源,促进原材料可持续供应,并且都启动了有关替代材料、降低消耗及循环利用的多领域高层次合作的综合型科研项目,一些成果已投入实际生产。

关键词：关键原材料战略金属稀土替代材料循环利用

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近50年来长江水资源特征变化分析

自然资源学报 2006年第4期21卷 501-506页

作者：戴仕宝杨世伦华东师范大学河口海岸学国家重点实验室

对长江水资源的特征如总量、用水量、水污染等的变化进行了分析,结果为:①1954—2004年流域平均降水量呈现下降趋势;长江上中游降水量的减少是导致宜昌、汉口水文站的径流量呈现下降趋势的主要原因,而大通水文站径流量呈上升趋势与汉口... 详细信息

对长江水资源的特征如总量、用水量、水污染等的变化进行了分析,结果为:①1954—2004年流域平均降水量呈现下降趋势;长江上中游降水量的减少是导致宜昌、汉口水文站的径流量呈现下降趋势的主要原因,而大通水文站径流量呈上升趋势与汉口—大通间降水量增加有关;大通站1950到2004年的最小、最大月均径流量均呈上升趋势;长江水资源总量的变化主要受控于气候的变化。②水污染逐渐加重,水质有恶化的趋势,工业污染的增加是主要原因。③年均用水量占入海径流量的5.7%左右,人类活动尚未对长江流域总的水量构成很大的影响。结论:①长江水资源总量尚未发生巨大的变化,但由人类活动所造成的污染已对水资源的利用构成了影响;②南水北调工程的实施和长江三角洲工业的发展,使得中国东部地区对长江干流水资源的依赖性增加,加大了长江水资源利用的风险。

关键词：水资源径流量水污染南水北调长江流域

Involvement of connexin 43 in acupuncture analgesia

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Chinese Medical Journal 2009年第1期122卷 54-60页

作者： HUANG Guang-ying ZHENG Cui-hong YU Wei-chang TIAN dai-shi WANG Wei Institute of Integrated Traditional and Western Medicine Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei 430030 China Department of Neurology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei 430030 China

Background Connexin 43 （Cx43） is one of the major components of human keratinocyte gap junctions. To study whether gap junctional intercellular communication participates in the transfer of acupoint signals and acupuncture analgesia, the expression of Cx43 was studied in Zusanli （ST36） acupoints compared with control non-acupoint regions in rats after acupuncture. In addition, Cx43 heterozygous gene knockout mice were used to further explore the relationship between Cx43 and acupuncture analgesia.Methods The expression of Cx43 was detected by immunohistochemistry, immunoblotting, and RT-PCR for the Cx43 protein and mRNA. The influence of the Cx43 gene knockout on acupuncture analgesia was measured by a hot plate and observing the writhing response on Cx43 heterozygous gene knockout mice. Results Immunohistochemistry showed abundant Cx43 expression in some cells in the skin and subcutaneous tissue of rat ST36 acupoints. The mRNA and protein levels of Cx43 in acupoints were significantly higher than those in the control points in the non-acupuncture group, and even more so after acupuncture. The hot plate and writhing response experiments showed that partial knockout of the Cx43 gene decreased acupuncture analgesia. Conclusion Cx43 expression and acupuncture analgesia showed a positive correlation.

关键词： connexin 43 gene knockout acupuncture analgesia hot plate test writhing response

Synthesis, characterization r）oly [LA-co-（GIc-alt-Lys）] for and biological evaluation of nerve regeneration scaffold

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Frontiers of Materials Science 2014年第1期8卷 95-101页

作者： Yi-Xia YIN Ji-Ling YI Li-Juan XIE Qiong-Jiao YAN Hong-Lian dai shi-Pu LI Biomedical Materials and Engineering Research Center Wuhan University of Technology Wuhan 430070 China

A novel nerve repairing material poly [LA-co.（GIc-alt-Lys）] （PLGL） was synthesized. The viability and growth of Schwann cells （SCs） co-cultured With poly （D, L- lactic acid）（PDLLA） films （control group） and PLGL films were evaluated by MTT assay and SEM observation. Then, contact angle measurement, histological assessment and enzyme-linked immunosorbent assay （ELISA） testing on inflammatory-related cyto- kines such as IL-10 and TGF-β1 were performed. The results showed that, compared with PDLLA, PLGL films possesses better hydrophilicity, biocompatibility, degradation property and less inflammatory reaction. The present study indicated that PLGL scaffolds would meet the requirements of artificial nerve scaffold and have a potential application in the fields of nerve regeneration.

关键词： Schwann cell (SC) poly [LA-co-(GIc, alt-Lys)] (PLGL) implantation inflammatory factor biocompatibility