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Estrogen inhibits lipid peroxidation after hypoxic-ischemic brain damage in neonatal rats

Neural Regeneration Research 2012年第31期7卷 2424-2431页

作者： Hui Zhu Xiao Han Dafeng Ji Guangming Lv Meiyu Xu Department of Pediatrics Affiliated Hospital of Nantong University Nantong 226001 Jiangsu Province China Department of Human Anatomy Institute of Neurobiology Medical School of Nantong University Jiangsu Key Laboratory ofNeuroregeneration Nantong 226001 Jiangsu Province China

Sprague-Dawley neonatal rats within 7 days after birth were used in this study. The left common carotid artery was occluded and rats were housed in an 8% O2 environment for 2 hours to establish a hypoxic-ischemic brain damage model. 17β-estradiol （1 × 10-5 M） was injected into the rat abdominal cavity after the model was successfully established. The left hemisphere was obtained at 12, 24, 48, 72 hours after operation. Results showed that malondialdehyde content in the left brain of neonatal rats gradually increased as modeling time prolonged, while malondialdehyde content of 17β-estrodial-treated rats significantly declined by 24 hours, reached lowest levels at 48 hours, and then peaked at 72 hours after injury. Nicotinamide-adenine dinucleotide phosphate histochemical staining showed the nitric oxide synthase-positive cells and fibers dyed blue/violet and were mainly distributed in the cortex, hippocampus and medial septal nuclei. The number of nitric oxide synthase-positive cells peaked at 48 hours and significantly decreased after 17β-estrodial treatment. Our experimental findings indicate that estrogen plays a protective role following hypoxic-ischemic brain damage by alleviating lipid peroxidation through reducing the expression of nitric oxide synthase and the content of malondialdehyde.

关键词： hypoxic-ischemic encephalopathy hypoxic-ischemic brain damage estrogen malondialdehyde free radical nitric oxide synthase lipid peroxidation neonatal rats neuroprotection neural regeneration

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Biotinylated dextran amine anterograde tracing of the canine corticospinal tract

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Neural Regeneration Research 2012年第11期7卷 805-809页

作者： Xiao Han Guangming Lv Huiqun Wu Dafeng Ji Zhou Sun Yaofu Li Lemin Tang Department of Human Anatomy Institute of Neurobiology Jiangsu Key Laboratory of Neuroregeneration Medical School Nantong University Nantong 226001 Jiangsu Province China Department of Medical Informatics Institute of Digital Medicine Medical School Nantong University Nantong 226001 Jiangsu Province China Department of Medical Image Engineering Medical School Nantong University Nantong 226001 Jiangsu Province China

In this study, biotinylated dextran amine （BDA） was microinjected into the left cortical motor area of the canine brain. Fluorescence microscopy results showed that a large amount of BDA-labeled pyramidal cells were visible in the left cortical motor area after injection. In the left medulla oblongata, the BDA-labeled corticospinal tract was evenly distributed, with green fluorescence that had a clear boundary with the surrounding tissue. The BDA-positive corticospinal tract entered into the right lateral funiculus of the spinal cord and descended into the posterior part of the right lateral funiculus, close to the posterior horn, from cervical to sacral segments. There was a small amount of green fluorescence in the sacral segment. The distribution of BDA labeling in the canine central nervous system was consistent with the course of the corticospinal tract. Fluorescence labeling for BDA gradually diminished with time after injection. Our findings indicate that the BDA anterograde tracing technique can be used to visualize the localization and trajectory of the corticospinal tract in the canine central nervous system.

关键词： biotinylated dextran amine corticospinal tract anterograde tracing fluorescence canine

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Three-dimensional reconstruction of human spinal cord based on histological serial sections

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Neural Regeneration Research 2008年第7期3卷 697-700页

作者： Guangming Lü Huiqun Wu Lemin Tang Xiao Han Dafeng Ji Xiaosong Gu Department of Anatomy Institute of Neurobiology Jiangsu Province Key Laboratory of Neural Regeneration Medical School of NantongUniversity Nantong 226001 Jiangsu Province China Laboratory of lmage Engineering Medical School of Nantong University Nantong 226001 Jiangsu Province China

BACKGROUND： It is not possible to reconstruct the inner structure of the spinal cord, such as gray matter and spinal tracts, from the Visual Human Project database or CT and MRI databases, due to low image resolution and contrast in macrosection images. OBJECTIVE： To explore a semi-automatic computerized three-dimensional （3D） reconstruction of human spinal cord based on histological serial sections, in order to solve issues such as low contrast. DESIGN, TIME AND SETTING： An experimental study combining serial section techniques and 3D reconstruction, performed in the laboratory of Human Anatomy and Histoembryology at the Medical School of Nantong University during January to April 2008. SETTING： Department of Anatomy, Institute of Neurobiology, jiangsu Province Key Laboratory of Neural Regeneration, Laboratory of Image Engineering. MATERIALS： A human lumbar spinal cord segment from fresh autopsy material of an adult male. METHODS： After 4% paraformaldehyde fixation for three days, serial sections of the lumbar spinal cord were cut on a Leica cryostat and mounted on slides in sequence, with eight sections aligned separately on each slide. All sections were stained with Luxol Fast Blue to reveal myelin sheaths. After gradient dehydration and clearing, the stained slides were coverslipped. Sections were observed and images recorded under a light microscope using a digital camera. Six images were acquired at x25 magnification and automatically stitched into a complete section image. After all serial images were obtained, 96 complete serial images of the human lumbar cord segment were automatically processed with ＂Curves＂, ＂Autocontrast＂, ＂Gray scale 8 bit＂, ＂Invert＂, ＂Image resize to 50%＂ steps using Photoshop 7.0 software. All images were added in order into 3D-DOCTOR 4.0 software as a stack, where serial images were automatically realigned with neighboring images and semi-automatically segmented for white matter and gray matter. Finally, simple surface and volume reconstruction were completed on a person

关键词： Luxol Fast Blue reconstruction serial sections surface three-dimensional visible human project volume

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Fluoro-ruby retrograde tracing and three-dimensional visualization of the corticospinal tract in the guinea pig

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Neural Regeneration Research 2012年第5期7卷 363-367页

作者： Xiao Han Lulian Xu Yue Wu Hua Xun jinxiu Pan Yingying Huang Dafeng Ji Huiqun Wu Guangming Lv Lemin Tang Department of Human Anatomy Institute of Neurobiology Jiangsu Key Laboratory of Neuroregeneration Medical School NantongUniversity Nantong 226001 Jiangsu Province China Medical School Nantong University Nantong 226001 Jiangsu Province China Department of Medical Informatics Medical School Nantong University Nantong 226001 Jiangsu Province China Department of Imaging Engineering Medical School Nantong University Nantong 226001 Jiangsu Province China

Fluoro-ruby was injected into the posterior funiculus of the spinal cord in the cervical （C5-T2） and lumbar （L3-6） segments of adult guinea pigs. The spinal cord was cut into serial frozen sections. The Fluoro-ruby labeling was clearly delineated from the surrounding structure. The labeling traversed the cervical, thoracic and lumbar segments, and was located on the ventral portion of the posterior funiculus on the injected side, proximal to the intermediate zone of the dorsal gray matter. The fluorescence area narrowed rostro-caudally. The spinal cord, spinal cord gray matter and corticospinal tract were reconstructed using 3D-DOCTOR 4.0 software, resulting in a robust three-dimensional profile. Using functionality provided by the reconstruction software, free multi-angle observation and sectioning could be conducted on the spinal cord and corticospinal tract. Our experimental findings indicate that the Fluoro-ruby retrograde fluorescent tracing technique can accurately display the anatomical location of corticospinal tract in the guinea pig and that three-dimensional reconstruction software can be used to provide a three-dimensional image of the corticospinal tract.

关键词： corticospinal tract Fluoro-ruby retrograde fluorescent tracing technique three-dimensional reconstruction guinea pig

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Application of Customized Navigated Template for Percutaneous Radiofrequency Thermocoagulation Treatment of Primary Trigeminal Neuralgia

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Chinese Medicine 2015年第3期6卷 175-180页

作者： Peng Wang Tiebao Gu Zefeng Zhang Huiqun Wu Dafeng Ji Department of Pediartic Orthopaedic Nantong Rich Hospital Affiliated to Yangzhou University Yangzhou China jiangsu ZhouKe Medical Instrument Technological Co. Ltd. Nantong China Department of Pain Nantong Convalescence Hospital Affiliated to Nantong University Nantong China Medical College Affiliated to Nantong University Nantong China Digital Medical Center Fudan University Shanghai China

Objective: To investigate the successful rate and accuracy of percutaneous radiofrequency thermocoagulation (PRT) for treatment of primary trigeminal neuralgia (PTN) with customized navigated template via three dimensional (3D) printing technique. Methods: 65 patients with PTN were recruited from January 2014 to March 2015 and randomly divided into two groups: template group (n = 28) and traditional group (n = 37). The patients in traditional group received PRT under guidance of C-arm fluoroscopy, while the ones in template group were treated with customized navigated templates. The data of time, depth and accuracy rate of puncture, the average effective dose equivalent of radiation, complications after operation were collected and analyzed. Results: No intra-operative failures occurred in the template group: the pain was alleviated immediately after operation. Accuracy rate of the template group was 100% while 96% was achieved in traditional group. However, the average time of puncture by the template was significantly reduced compared with traditional group (2.37 ± 0.64 minutes and 24.2 ± 6.55 minutes, respectively;P 0.05). No complications were observed in template group while several complications such as blooding, leakage of cerebrospinal fluid and dizziness were observed in traditional group. Conclusion: The application of customized template is advocated for improving the accuracy of PRT.

关键词： Primary Trigeminal Neuralgia 3D Printing Radiofrequency Customized Template

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三维二值掩模零化法结合体素绘制法切割及渲染CT与MRI体积数据

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中国医学影像技术 2023年第11期39卷 1721-1725页

作者：田野戴燕飞季达峰南通大学医学院医学信息学系江苏南通226001 南通大学附属医院分院影像科江苏南通226001

目的观察三维二值掩模零化法联合体素绘制法对于切割及渲染CT与MRI体积数据的作用。方法利用Matlab软件将DICOM形式头颈部CT、腹部平扫CT、正常头部MR T1WI及脑胶质瘤T1WI转化为体积数据,以半自动或手动方法获得相应掩模图像;基于原... 详细信息

目的观察三维二值掩模零化法联合体素绘制法对于切割及渲染CT与MRI体积数据的作用。方法利用Matlab软件将DICOM形式头颈部CT、腹部平扫CT、正常头部MR T1WI及脑胶质瘤T1WI转化为体积数据,以半自动或手动方法获得相应掩模图像;基于原始掩模以零化法在x、y、z轴方向对体积数据进行正向与反向切割,结合三维体素绘制法对切割后的模型进行渲染。结果所获目标结构原始掩模可在体素绘制后突出显示CT及MRI数据中的相应解剖结构。基于体素绘制法实现了以三维二值掩模零化法与原体数据点积后切割模型,结合手动输入不同阈值进行切割可显示脑胶质瘤及其毗邻结构。结论三维二值掩模零化法联合体素绘制法可结合不同层面CT与MRI体积数据显示其内部结构。

关键词：体层摄影术 X线计算机磁共振成像零化法三轴切割体素渲染

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CT及MR血管造影图像融合评估原发性三叉神经痛患者三叉神经入脑干区血管、神经及骨性结构的关系

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中国医学影像技术 2022年第7期38卷 995-999页

作者：徐嘉季达峰吕广明南通大学医学院江苏南通226001 无锡市第二人民医院神经外科江苏无锡214000

目的观察CT及MR血管造影(MRA)双模态图像融合评估原发性三叉神经痛(PTN)患者三叉神经入脑干区(REZ)血管、神经及骨性结构的关系的价值。方法回顾性分析10例PTN患者头部CT及MRA,将数据三维可视化后,分别于CT与MRA图中选取9个解剖标记点... 详细信息

目的观察CT及MR血管造影(MRA)双模态图像融合评估原发性三叉神经痛(PTN)患者三叉神经入脑干区(REZ)血管、神经及骨性结构的关系的价值。方法回顾性分析10例PTN患者头部CT及MRA,将数据三维可视化后,分别于CT与MRA图中选取9个解剖标记点进行配准,根据配准后标记点的欧氏距离选出5个融合标记点用于图像融合;计算融合标记点的配准误差(FRE),评估图像融合精度。基于MRA数据重建脑底动脉及神经模型,观察REZ处血管与三叉神经根的空间位置关系,判定PTN责任血管;测量并比较患侧与对侧责任血管与三叉神经根、三叉神经压迹及卵圆孔之间距离的差异。结果10例图像融合精度均良好,标记点FRE为1.862~3.156 mm,PTN责任血管均为小脑上动脉。患侧小脑上动脉与三叉神经压迹距离[(0.531±0.153)mm]及与卵圆孔的距离[(20.955±1.147)mm]均小于对侧[(2.573±1.050)mm、(24.864±1.807)mm,P均<0.001],与三叉神经根的距离[(5.024±1.063)mm]与对侧差异无统计学意义[(5.971±1.292)mm,P=0.081]。结论CT及MRA图像融合有助于定量观察PTN患者REZ血管与骨性结构及三叉神经根的毗邻关系。

关键词：三叉神经痛体层摄影术,X线计算机磁共振成像图像融合

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In situ growth of polydopamine on surface of covalent organic frameworks under the catalysis of acid phosphatase for dopamine detection

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Chinese Chemical Letters 2021年第11期32卷 3368-3371页

作者： Fei Qu Ziwei Guo Dafeng Jiang Xian-En Zhao The Key Laboratory of Life-Organic Analysis Qufu Normal UniversityQufu 273165China The Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine Qufu Normal UniversityQufu 273165China Department of Physical and Chemical Testing Shandong Center for Disease Control and PreventionJi'nan 250014China

Dopamine(DA)is easy to be oxidized and polymerizes to form polydopamine(pDA)in alkaline conditions,while the synthesis is usually time-consuming(48 h).Herein,the polymerization of DA is completed with 4 h under the catalysis of acid phosphatase(ACP).The high efficiency makes the detection of DA feasibility based on the self-polymerization of DA.In this assay,pDA is grown in situ on the surface of covalent organic frameworks(COFs),and then the fluorescence of COFs is quenched significantly.The linear range of DA is achieved from 0.5-50μmol/L with a detection limit of 0.16μmol/L.The detection of DA is not interfered with uric acid,ascorbic acid,and some phenolic compounds,because these substances cannot polymerize in the presence of ACP.Moreover,benefiting from the good sensitivity and selectivity,DA has been successfully determined by this strategy in human urine samples with satisfactory recoveries.

关键词： Fluorescence Enzyme Polydopamine Dopamine Acid phosphatase

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基于网络药理学及动物实验研究温肾宣痹汤抗骨质疏松的机制

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中国药理学通报 2024年第2期40卷 344-351页

作者：王海平袁兆丰夏天卫张超沈计荣南京中医药大学附属医院/江苏省中医院骨伤科江苏南京210029 盐城市大丰中医院骨伤科江苏盐城224100

目的应用网络药理学、分子对接及体内实验技术,预测以及验证温肾宣痹汤(wenshen xuanbi tang,WSXBT)抗骨质疏松(osteoporosis,OP)的作用机制。方法利用网络药理学筛选WSXBT治疗OP的关键成分及核心靶点,Metascape数据库对核心靶点进行基... 详细信息

目的应用网络药理学、分子对接及体内实验技术,预测以及验证温肾宣痹汤(wenshen xuanbi tang,WSXBT)抗骨质疏松(osteoporosis,OP)的作用机制。方法利用网络药理学筛选WSXBT治疗OP的关键成分及核心靶点,Metascape数据库对核心靶点进行基因本体论(gene ontology,GO)功能分析和京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)通路富集分析,AutoDockTools 1.5.7软件进行分子对接模拟关键活性成分与核心靶点的结合活性。切除大鼠双侧卵巢建立OP大鼠模型,研究WSXBT对OP大鼠的药效及验证相关靶点通路。酶联免疫吸附(ELISA)法检测血清中OPG、PINP、RANKL水平,生物力学测试仪测定大鼠股骨生物力学,Micro-CT检测股骨骨密度,进而通过Western blot法检测PI3K和Akt的蛋白表达水平。结果筛选出WSXBT活性成分156个,涉及229个潜在作用靶点,筛选核心靶点23个,共涉及145条信号通路;分子对接结果显示,槲皮素、异补骨脂黄酮、β-谷甾醇等关键成分与PIK3R1、AKT1核心靶点均拥有良好的结合能力。体内实验结果表明,WSXBT可明显升高OP大鼠骨密度,改善OP大鼠骨组织微结构,提高大鼠股骨生物力学,增加大鼠PINP表达及OPG/RANKL比值,Western blot结果显示,WSXBT可明显升高p-PI3K/PI3K、p-Akt/Akt比值。结论WSXBT可能通过PI3K/Akt信号通路及升高OPG/RANKL比值改善绝经后OP大鼠骨密度。

关键词：网络药理学分子对接温肾宣痹汤骨质疏松 p-PI3K/PI3K

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基于MATLAB的微创骨科穿刺规划平台构建与临床验证

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中国数字医学 2023年第2期18卷 86-92页

作者：季达峰张长青张愈峰南通大学医学院数字医学中心江苏南通226001 河北医科大学南通大学附属医院分院骨科

目的:探讨MATLAB的影像微创骨科穿刺规划平台建立所需技术及临床应用指标。方法:利用MATLAB建立图像导入、三维重建与定位平台。选取重度椎间盘脱出患者40例,随机分为试验组与对照组各20例。试验组所得CT数据导入三维骨科微创定向系统(3... 详细信息

目的:探讨MATLAB的影像微创骨科穿刺规划平台建立所需技术及临床应用指标。方法:利用MATLAB建立图像导入、三维重建与定位平台。选取重度椎间盘脱出患者40例,随机分为试验组与对照组各20例。试验组所得CT数据导入三维骨科微创定向系统(3DDisplay2.0)中进行三维重建,利用平台进行穿刺点定位、角度与穿刺深度测量;对照组CT数据打印成醋酸酯胶片,在术中进行定位、角度与深度测量。利用C臂机进行术中透视确认穿刺效果,记录穿刺耗时与术中并发症并做统计学处理。结果:建立起图像导入,三维显示与断层定位3个主要模块;试验组与对照组穿刺时程卡方值为14.546>10.828(P=0.001),透视次数卡方值为28.972>10.828(P=0.001);穿刺时程与透视次数的t值分别为6.022>4.144(P=0.001),10.314>4.144(P=0.001);试验组术中出现酸胀与疼痛为2例(10%),低于对照组的15例(75%)。结论:以MATLAB为基础的模块设计可有效对CT术前影像进行重构、定位与三维重建;病灶、棘突与穿刺点的三角关系可对穿刺方向进行有效修正;基于三维可视与夹角修正的骨科微创引导平台在椎间孔镜穿刺中可显著降低穿刺时程,减少透视次数与术中并发症。

关键词：医学影像骨科微创穿刺穿刺技术指标计算机辅助手术临床验证

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