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Crystallization and preliminary X-ray analysis of an anti-insect toxin BmK 16 from Buthus martensii Karsch scorpion

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Chinese Science Bulletin 2000年第16期45卷 1489-1492页

作者： Rongjin Guan Miao wang Xinqi Liu dacheng wang Acad Sinica Inst Biophys Beijing 100101 Peoples R China

An excitatory anti-insect toxin from BmK scorpion, named BmK 16, has been purified and crystallized recently. Two crystal forms have been obtained by using ammonium sulfate as precipitant at different pH, one of which is single crystal. Diffraction data have been collected for the single crystal on a Mar Research 345 Image Plate. The analyses of the data show that the crystal can diffract to 0.28 nm resolution and belongs to the space group C2 with unit cell parameters a = 4.65 nm, b = 8.52 nm, c= 3.26 nm, α= γ= 90oooooooo, β= 110.5°. There are 2 molecules per asymmetric unit according to its unit cell volume and molecular weight.

关键词： scorpion neurotoxin anti-insect toxin crystallization crystal data.

Empowering magnetic strong coupling and its application for nonlinear refractive index sensing

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Nano Research 2022年第8期15卷 7604-7613页

作者： Song Sun Dong Li dacheng wang Zheng Feng Wei Tan Lin Wu Microsystem and Terahertz Research Center China Academy of Engineering PhysicsChengdu 610200China Institute of Electronic Engineering China Academy of Engineering PhysicsMianyang 621999China Science Mathematics and Technology(SMT)Singapore University of Technology and Design(SUTD)8 Somapah RoadSingapore 487372Singapore Institute of High Performance Computing A*STAR(Agency for ScienceTechnology and Research)1 Fusionopolis Way#16-16 ConnexisSingapore 138632Singapore

In the context of quantum strong coupling,the magnetic dipole(MD)emitters are largely overlooked due to the rarity of MD source and the non-magnetic nature of matters at high *** on a semi-classic model,we theoretically demonstrate magnetic strong coupling between an MD cluster(Er^(3+):4 I13/2→4 I15/2 transition at 1,550 nm)and an antenna-in-cavity *** is found that placing the plasmonic diabolo/s-diabolo nanoantenna,which supports strong electric/magnetic dipole mode,inside a dielectric cavity could largely improve the strong coupling coefficient while suppressing the cavity loss rate compared to the bare nanoantenna counterparts,empowering the magnetic quantum strong coupling at a level of 104 emitters,which is remarkable considering the weak MD dipole momentum and small hotspot region at high ***,the two Rabi resonance branches undergo highly asymmetrical changes upon a small variation on the environmental refractive index,which leads to an exotic exponential sensitivity profile by tracing the ratio between the two resonances *** proposed magnetic strong coupling for nonlinear refractive index sensing may add a new category to quantum plasmonic sensors.

关键词： nanoplasmonic cavity magnetic dipole emitter strong coupling Rabi-splitting sensing

Crystallization and preliminary crystallographic studies of insulin T_3R_3 states created in different conditions

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Chinese Science Bulletin 1998年第1期43卷 59-62页

作者： AI Xi ZENG Zonghao HUANG Peitang Jan Markussen wang dacheng Institute of Biophysics Chinese Academy of SciencesBeijing 100101China Institute of Biotechnology Academy of Military Medical SciencesBeijing 100071China Novo Research Insti tute Novo AlleDK_2880 BagsvaerdDenmark

In the presence of a series of small organic molecules including 1, 4 dioxane, DMF, isopropanol, rDNA human insulin, mutants A21 Asn→Ser and (A21 Asn→Ser, B27 Thr→Arg) human insulin have been crystallized in both Zn 2+ containing and Zn 2+ free system. Preliminary crystallographic analyses show that they all belong to T 3R 3 structural type. The results indicate that, except phenolic derivatives and lyotropic anions reported before, some other appropriate organic molecules can also induce the T 3R 3 conformation transition of insulin.

关键词： insulin T_3R_3 structure effector crystallization preliminary crystallographic study cell constants.

Crystallization and preliminary crystallographic analyses of human insulin mutant B9(Ser→Asp)in different aggregation forms

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Chinese Science Bulletin 1999年第9期44卷 796-799页

作者： Xinqi Liu Lei Jin Ying Zhang Markussen Jan dacheng wang Institute of Biophysics Chinese Acndemy of Sciences Beijins China Novo Research Institute Novo Alle Denmark

B9(Ser→Asp) insulin, one of the fast-acting insulin mutants produced by protein engineering, has been crystallized. Three crystal forms have been obtained, which belong to orthorhombic, tetragonal and rhombohedral system, respectively. Each crystal form contains different aggregation units of the mutant, i.e. dimer or hexamer. The diffraction data of the former two have been collected beyond 0.2 nm resolution. The structural analyses and comparisons will provide some information about insulin’s self-association and the structural basis of the fast absorption produced from the mutation.

关键词： insulin mutant. B9Asp insulin crystallization crystallographic parameter.

Structural basis of the ultrasensitive calcium indicator GCaMP6

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Science China(Life Sciences) 2014年第3期57卷 269-274页

作者： DING JingJin LUO Andrew F. HU LiYan wang dacheng SHAO Feng National Laboratory of Biomacromolecules Institute of BiophysicsChinese Academy of Sciences National Institute of Biological Sciences

GCaMP is one of the most widely used calcium indicators in neuronal imaging and calcium cell biology. The newly developed GCaMP6 shows superior brightness and ultrasensitivity to calcium concentration change. In this study, we determined crystal structures of CaZ＋-bound GCaMP6 monomer and dimer and presented detailed structural analyses in comparison with its par- ent version GCaMP5G. Our analyses reveal the structural basis for the outperformance of this newly developed Ca2＋ indicator. Three substitution mutations and the resulting changes of local structure and interaction explain the ultrasensitivity and in- creased fluorescence intensity common to all three versions of GCaMP6. Each particular substitution in the three GCaMP6 is also structurally consistent with their differential sensitivity and intensity, maximizing the potential of using GCaMP6 in solving diverse problems in neuronal research and calcium signaling. Our studies shall also be beneficial to further structure-guided optimization of GCaMP and facilitate the design of novel calcium indicators.

关键词： GCaMP6 calcium indicator structural basis ultrasensitivity

Middle Triassic(Anisian)Conodont Biostratigraphy at the Shaiwa Section,Guizhou,South China

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Journal of Earth Science 2021年第3期32卷 592-615页

作者： Binxian Qin Martyn Lee Golding Haishui Jiang Yan Chen Muhui Zhang Li Kang dacheng wang Jinling Yuan State Key Laboratory of Biogeology and Environmental Geology China University of GeosciencesWuhan 430078China School of Earth Sciences China University of GeosciencesWuhan 430074China Geological Survey of Canada Vancouver V6B 5J3Canada Hubei Institute of Geosciences Wuhan 430034China

The Nanpanjiang Basin is a key area for paleontological and biostratigraphical study of the Middle *** we studied Middle Triassic conodonts from a well-exposed section,the Shaiwa Section,which is located at the northwest end of the Nanpanjiang Basin.A total of six Anisian conodont zones are recognized;in ascending order,they are:the Nicoraella germanica Zone,the Nicoraella kockeli Zone,the Paragondolella bulgarica Zone,the Neogondolella constricta Zone,the Neogondolella cornuta Zone,and the Paragondolella excelsa Zone,*** first occurrence of Nicoraella kockeli defines the Bithynian-Pelsonian *** Pelsonian-Illyrian boundary is defined by the first occurrence of Neogondolella *** Anisian-Ladinian boundary cannot be recognized at the Shaiwa Section due to the absence of conodont indicative of the ***,the new conodont data indicate that the uppermost strata could be very close to the *** abrasion of conodont surfaces provides evidence for demonstrating reworking at the Shaiwa Section,which makes some conodonts possess a longer stratigraphic range than previously *** variation in relative abundance between blade-shaped conodonts and platform conodonts indicates that segminiplanate elements probably preferred deeper and oxygenated environments whereas a restricted marine environment is more suitable for segminate elements.

关键词： conodont biostratigraphy Middle Triassic Shaiwa Section Nanpanjiang Basin

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Suppression of ETI by PTI priming to balance plant growth and defense through an MPK3/MPK6-WRKYs-PP2Cs module

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Molecular Plant 2023年第5期16卷 903-918页

作者： dacheng wang Lirong Wei Ting Liu Jinbiao Ma Keyi Huang Huimin Guo Yufen Huang Lei Zhang Jing Zhao Kenichi Tsuda Yiming wang Key Laboratory of Biological Interactions and Crop Health Department of Plant PathologyNanjing Agricultural UniversityNanjing 210095China Key Laboratory of Integrated Management of Crop Disease and Pests Ministry of EducationNanjing Agricultural UniversityNanjingChina State Key Laboratory of Agricultural Microbiology Hubei Hongshan LaboratoryHubei Key Lab of Plant PathologyCollege of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanChina

Pattern-triggered immunity(PTI)and effector-triggered immunity(ETI)are required for host defense against *** PTI and ETI are intimately connected,the underlying molecular mechanisms remain *** this study,we demonstrate that flg22 priming attenuates Pseudomonas syringae *** DC3000(Pst)AvrRpt2-induced hypersensitive cell death,resistance,and biomass reduction in ***-activated protein kinases(MAPKs)are key signaling regulators of PTI and *** absence of MPK3 and MPK6 significantly reduces pre-PTI-mediated ETI suppression(PES).We found that MPK3/MPK6 interact with and phosphorylate the downstream transcription factor WRKY18,which regulates the expression of AP2C1 and PP2C5,two genes encoding protein ***,we observed that the PTI-suppressed ETI-triggered cell death,MAPK activation,and growth retardation are significantly attenuated in wrky18/40/60 and ap2c1 pp2c5 *** together,our results suggest that the MPK3/MPK6-WRKYs-PP2Cs module underlies PES and is essential for the maintenance of plant fitness during ETI.

关键词： PAMP-triggered immunity effector-triggered immunity MAPK,WRKY18 protein phosphatase

Farrerol as an Innovative Antivirulence Agent Disrupting Virulence Factors to Combat Foodborne Pathogenicity in Staphylococcus aureus

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Food Science and Human Wellness 2024年

作者： Hangqian Yu Yuxin Zhang Yuxin Yang Xiaoping Zhou dacheng wang State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases， Key Laboratory for Zoonosis Research of the Ministry of Education， College of Veterinary Medicine， Jilin University College of Animal Science， Jilin University College of Basic Medical Sciences， Jilin University School of Pharmaceutical Science， Jilin University

The overuse of antibiotics has accelerated the emergence of resistant bacteria， posing critical threats to food safety and public health. This challenge underscores the urgent need for alternative strategies， such as antivirulence therapy， which disarms pathogens by targeting virulence factors rather than inhibiting bacterial growth. In this study， we identified farrerol， a flavonoid， as a potent inhibitor of the caseinolytic peptidase P （ClpP） protease， a key virulence regulator in Staphylococcus aureus， with an IC50of 19.33 μg/mL. Farrerol significantly disrupts virulence factor secretion and reduces pathogenicity， demonstrating its potential to control Staphylococcus aureus-induced foodborne illnesses. Mechanistic studies using fluorescence resonance energy transfer （FRET）， thermal shift assay （TSA）， cellular thermal shift assay （CETSA）， fluorescence quenching， and molecular docking revealed that farrerol interacts with ClpP via key residues （GLY-127， GLN-132， and ASP-170）. In vivo， farrerol reduced the bacterial load and improved survival in a Galleria mellonella infection model. Our findings demonstrate that farrerol is an effective antivirulence agent， offering an innovative approach for the food industry to control Staphylococcus aureus-induced foodborne infections. This approach holds great potential for enhancing food safety and mitigating antibiotic resistance.

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Complementary bilayer metasurfaces for enhanced terahertz wave amplitude andphase manipulation

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光电工程 2017年第1期44卷 77-81页

作者： dacheng wang Yandong Gong Minghui Hong Department of Electrical and Computer Engineering National University of Singapore

Manipulation of terahertz wave by metasurfaces has shown tremendous potential in developing compact and functional terahertz optical ***,we propose complementary bilayer metasurfaces for enhanced terahertz wave amplitude and phase *** metasurfaces are composed of one layer of metal cut-wire arrays and one layer of their complementary aperture arrays separated by a dielectric *** the near-field coupling between transverse magnetic resonances in the metal apertures and electric resonances in the metal cut-wires,the structures can manipulate the cross polarization conversion and phase dispersion of terahertz ***,the designed metasurfaces demonstrate a phase delay of 180°between two orthogonal axes with the same transmission amplitude between 0.70 and 1.0 THz,enabling a 45°broadband polarization *** the metal cut-wires are rotated with respect to the apertures or the thickness of the dielectric spacer is changed,the amplitude and phase dispersion of the transmitted terahertz wave can be *** complementary coupled bilayer metasurfaces offer a new method to control the amplitude and phase dispersion of terahertz wave and promise great potential for applications in terahertz meta-devices.

关键词： metasurfaces terahertz polarization wave manipulation