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Effects of interleukin-10 on activation and apoptosis of hepatic stellate cells in fibrotic rat liver

World Journal of Gastroenterology 2006年第12期12卷 1918-1923页

作者： Li-Juan Zhana wei-da zheng Mei-Na Shi Department of Gastroenterology Union Hospital of Fujian Medical University.Fuzhou 350001.Fujian Province.China

AIM： TO study the effects of interleukin-10 （IL-10） on the expression of o-smooth muscle actin （α-SMA）, nuclear factor-κB（NF-κB） and Fas/Fas ligand （FasL） in hepatic stellate cells of experimental rats with hepatic fibrosis. METHODS： Sixty clean SD rats were randomly divided into control group （group N）, liver fibrotic group （group C） and IL-10 treatment group （group I）. Control group received intraperitoneal injection of saline （2ml·kg^-1）, twice a week. Fibrotic group was injected intraperitoneally with 50% carbon tetrachloride （CCh）（2 ml·kg^-1）, twice a week. IL-10 treatment group was given IL-10 at a dose of 4 pg·kg^-1 20 minutes before CCl4 administration from the third week. Hepatic stellate cells （HSCs） were isolated from these rats at the seventh and eleventh weeks during the course of liver fibrosis, respectively. The expression of α-SMA and NF-κB in HSCs was measured by S-P immunohistochemistry. The expression of Fas and FasL mRNA was measured by RT-PCR. Furthermore, liver tissues were harvested from three groups at the same time. RESULTS： The CCh- induced experimental rat hepatic fibrosis model was established successfully. The purity of extracted hepatic stellate cells was about 95% and the yield of hepatic stellate cells was 1.2-2.3×10^6/g liver tissue averagely. The positive expression of α-SMA and NF-κB was 36.5% and 28.5% respectively in group N. The positive levels of α-SMA and NF-κB were increased significantly in group C compared to group N （P〈0.01）. The positive signals decreased significantly （P〈0.05） in group I. In the 11^th week, the HSCs of group I became round with visible pyknotic nuclei. The expression of NF-κB in group C was significantly increased in a timedependentmanner （P〈0.01）, but there was no difference in the α-SMA expression （P〉0.05）. The mRNA of Fas and FasL in group C was significantly increased in a timedependent manner compared to that in control group. After treated with IL-10, the expression level of Fas and FasL was h

关键词： Liver fibrosis Hepatic stellate cell Znterleuldn-10 α-smooth muscle actin Nuclear factor-κB Rat

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Effect of IL-10 on the expression of HSC growth factors in hepatic fibrosis rat

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World Journal of Gastroenterology 2005年第31期11卷 4788-4793页

作者： Mei-Na Shi wei-da zheng Li-Juan Zhang Zhi-Xin Chen Xiao-Zhong Wang Department of Gastroenterology Union Hospital of Fujian Medical University Fuzhou 350001 Fujian Province China

AIM： To study the effect of IL-10 on the expression of growth factors - transforming growth factor-β1 （TGF-β1）, epidermal growth factor （EGF）, hepatocyte growth factor （HGF）and platelet-derived growth factor （PDGF） of hepatic stellate cells （HSCs） of hepatic fibrosis rat and the anti-fibrogenic role of exogenous IL-10. METHODS： Hepatic fibrosis was induced by CCI4 administration intra-peritoneally. Sixty clean male Sprague-dawley （SD） rats were randomly divided into three groups： normal control group （GN, 8 rats）, hepatic fibrosis model group （GC, 28 rats） and IL-10 treated group （GI, 24 rats）. At the beginning of the 7^th and 11^th wk, rats in each group were routinely perfused with pronase E and type IV collagenase through a portal vein catheter and the suspension obtained from the liver was spun by centrifugation with 11% Nycodenz density gradient to isolate HSCs. Histological examination was used to determine the degree of hepatic fibrosis. RT-PCR was employed to analyze mRNA expression from freshly isolated cells. Immunocytochemistry was performed to detect protein expression in primary cultured HSCs. RESULTS： Rat hepatic fibrosis was developed with the increase of injection frequency of CCl4, and HSCs were successfully isolated. At the 7^th and 11^th wk, TGF-β1, EGF, and HGF mRNA in GC increased obviously compared with GN （P = 0.001/0.042, 0.001/0.001, 0.001/0.001） and GI （P= 0.001/0.007, 0.002/0.001, 0.001/0.001）. For TGF-β1, no difference was observed between GI and GN. For EGF, mRNA level in GI increased compared with GN during the 7^th wk （P= 0.005） and 11^th wk （P= 0.049）. For HGF, mRNA level in GI decreased compared with GN at the 7^th wk （P = 0.001） and 11^th wk （P= 0.021）. Between these two time points, TGF-β1 expression at the 7^th wk was higher than that of the 11^th wk （P = 0.049）, but for EGF, the former was lower than the latter （P = 0.022）. As for PDGF mRNA, there was no significant difference between thesegroups, but difference seemed to exist i

关键词： Hepatic fibrosis Hepatic stellate cells Interleukin-10 Transforming growth factor-131 Epidermal growth factor Hepatocyte growth factor Platelet-derived growth factor

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Fabrication of alginate-based microspheres with cellular structure for tuning ammonium dinitramide performance

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Defence Technology（防务技术） 2023年第10期28卷 111-120页

作者： Dun-ju Wang Xu Zhou Yao-feng Mao Xin Wang Ye-ming Huang Rui-hao Wang da-wei zheng Co-Innovation Center for New Energetic Materials Southwest University of Science and TechnologyMianyang621010China China Academy of Engineering Physics Institute of Chemical MaterialsMianyang621900PR China

Recently,an emerging category green of energetic material ammonium dinitramide(ADN)has exhibited promising application in propellants due to its outstanding merits in energy release and environmental *** can be considered to substitute traditional oxidizer of ammonium perchlorate(AP)in military systems and *** this paper,a novel spherical energetic composite ADN/copper alginate(CA)with a microporous structure was designed and prepared by the W/O gel emulsion method,and a desirable porous microsphere structure was *** characterization techniques were used to investigate the structure and properties of ADN/CA *** results showed that ADN crystals were homogeneously encapsulated in an alginate-gel *** decomposition temperature was reduced to 151.7℃compared to ADN,while the activation energy of them was reduced from 129.73 k J/mol(ADN)to 107.50 k J/mol(ADN/CA-4).In addition,as-prepared samples had lower impact and frictional sensitivity than *** mechanism of sensitivity reduction and decomposition are also ***-volume combustion tests show that peak pressure of the ADN/CA-4 achieves 253.4 k Pa and pressurization rate of 2750.4 k Pa/***,this has a promising application in improving the combustion performance and safety performance of solid propellants.

关键词： Ammonium dinitramide Sodium alginate Microspheres High reactivity Energetic materials

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Relationship between transforming growth factorβ1 and antifibrotic effect of interleukin-10

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World Journal of Gastroenterology 2006年第15期12卷 2357-2362页

作者： Mei-Na Shi Yue-Hong Huang wei-da zheng Li-Juan Zhang Zhi-Xin Chen Xiao-Zhong Wang Department of Gastroenterology Union Hospital of Fujian Medical UniversityFuzhou 350001 Fujian Province China

AIM： To study the effect of interleukin-10 （IL-10） on the expression of transforming growth factor β1 （TGF-β1） in hepatic fibrosis rats and the anti-fibrotic role of exogenous IL-10. METHODS： Hepatic fibrosis was induced by carbon tetrachloride administered （CCh） intraperitoneally. The experiment was performed in two stages. In the first stage, 60 SD rats were divided randomly into normal control group I（GNI, n = 8）, hepatic fibrosis group（GC, n = 28）and IL-10 intervened group（GI, n = 24）. At the beginning of the 7^th and 11^th wk, hepatic stellate cells （HSCs） were isolated, reverse transcription-polymerase chain reation （RT-PCR） and immunocytochemistry were performed to detect the expression of TGF-β1 in HSCs. Histological examination was used to determine the degree of hepatic fibrosis. In the second stage, 47 SD rats were divided randomly into normal control group 2 （GN2, n = 6）and CCh group（GZ, n = 41）. At the end of the 9th week, rats in GZ group were allocated randomly into model group（GM, n = 9）, IL-10 treatment group（GT, n = 9） and recovered group （GR, n = 9）. At the end of the 12^th week, all rats were sacrificed. RT-PCR and immuno- histochemistry were performed to detect the expression of TGF-β1 in liver tissue. ELISA was used to assay serum TGF-β1 levels. RESULTS： Hepatic fibrosis developed in rats with the increase of the injection frequency of CCI4. In the first stage, hepatic fibrosis developed and HSCs were isolated successfully. At the 7^th and 11^th week, TGF-β1 mRNA in GC group increased significantly compared with that in GN1（P = 0.001/0.042） and GI groups（P = 0.001/0.007）, whereas there was no significant difference between the two groups. The levels of TGF-β1 at the beginning of the 7^th wk was higher than that of the 11^th wk （P = 0.049）.Immunocytochemistry results of TGF-β1 were consistent with the above findings. In the second stage, TGF-β1 increased significantly in GM group compared to GN2. Alter treatment with IL-10, TGF-β1 declined obvio

关键词： Hepatic fibrosis Hepatic stellate cells Interleukin-10 Transforming growth factor-β1

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Down-regulation of eIF5A-2 prevents epithelial-mesenchymal transition in non-small-cell lung cancer cells

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第6期14卷 460-467页

作者： Guo-dong XU Xin-bao SHI Le-bo SUN Qing-yun ZHOU da-wei zheng Huo-shun SHI Yong-liang CHE Zi-shan WANG Guo-feng SHAO Department of Thoracic & Cardiovascular Surgery Lihuili HospitalNingbo Medical CenterAffiliated Hospital of Medical SchoolNingbo University Department of Thoracic & Cardiovascular Surgery Ningbo First HospitalAffiliated Hospital of Medical SchoolNingbo University

Background:Epithelial-mesenchymal transition(EMT) is believed to be the critical process in malignant tumor invasion and metastases,and has a great influence on improving the survival rate in non-small-cell lung cancer(NSCLC) *** studies suggested that eukaryotic initiation factor 5A-2(eIF5A-2) might serve as an adverse prognostic marker of *** detected eIF5A-2 in NSCLC A549 cells,and found that the invasive capability correlates with the eIF5A-2 ***:Transforming growth factor(TGF)-β1 was used to induce EMT in A549 *** blotting,immunofluorescence,wound healing assay,and transwell-matrigel invasion chambers were used to identify phenotype *** blotting was also used to observe changes of the expression of *** down-regulated the eIF5A-2 expression using an eIF5A-2 siRNA and identified the phenotype changes by western blotting and *** tested the change of migration and invasion capabilities of A549 cells by the wound healing assay and transwell-matrigel invasion ***:After stimulating with TGF-β1,almost all A549 cells changed to the mesenchymal phenotype and acquired more migration and invasion *** cells also had higher eIF5A-2 protein ***-regulation of eIF5A-2 expression with eIF5A-2 siRNA transfection could change the cells from mesenchymal to epithelial phenotype and decrease tumor cell migration and invasive capabilities ***:The expression of eIF5A-2 was up-regulated following EMT phenotype changes in A549 cells,which correlated with enhanced tumor invasion and metastatic ***,in the A549 cell line,the process of EMT phenotype change could be reversed by eIF5A-2 siRNA,with a consequent weakening of both invasive and metastatic capabilities.

关键词： Non-small-cell lung cancer(NSCLC) Epithelial-mesenchymal transition(EMT) Eukaryotic initiation factor 5A-2(eIF5A-2) Transforming growth factor(TGF)-β1 A549

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Therapeutic effect of interleukin-10 on CCI_4-induced hepatic fibrosis in rats

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World Journal of Gastroenterology 2006年第9期12卷 1386-1391页

作者： Yue-Hong Huang Hei-Na Shi wei-da zheng Li-Juan Zhang Zhi-Xin Chen Xiao-Zhong Wang Department of Gastroenterology Union Hospital of Fujian Medical University Fuzhou 350001 Fujian Province China

AIM： To study the therapeutic effect of exogenous interleuldn-10 on CCl4-induced hepatic fibrosis in rats and its passible mechanisms. METHODS： Fourty-seven SD rats were randomly divided into control group （group N） and CCl4-induced hepatic fibrosis model group （group C）. After CCl4 was given for 9 wk, the model group was divided into three groups. Rats in group H were put to death immediately, rats in group T were treated with IL-10 for another three wk and then put to death, rats in group R recovered after three weeks and were then killed. The degree of hepatic fibrosis was measured by HE staining and histological activity index （HAI）. Histological activity index （HAI）, change of collagen types Ⅰ and Ⅲ were measured by Picrosirius staining. The expression of TNF-α, HHP-2 and TIMP-1 in liver tissue was measured by S-P immunohis tochemistry.RESULTS： CCl4- induced experimental rat hepatic fibrosis model was established successfully. The degree of hepatic fibrosis was markedly lower in group T than in groups H and R, and there was no difference between the two groups. The expression of collagen types I and III was significantly suppressed in group T and was slightly suppressed in groups H and R. The positive levels of TNF-α, HHP-2 and TIHP-1 in group H increased significantly compared to those in group N （P〈0.01）. The positive signals decreased significantly in groups T and R （P〈0.01）, but positive score was significantly lower in group T than in group R （P〈 0.01）. CONCLUS10N： Exogenous IL-10 can reverse CCl4-induced hepatic fibrosis in rats. IL-10 may exert its reversible effects on hepatic fibrosis by blocking CCl4-induced inflammation, inhibiting expression of HHP-2 and TIMP-1 and promoting resolution of collagen types Ⅰ and Ⅲ.

关键词： Animals Carbon Tetrachloride Collagen Type I Collagen Type III Immunohistochemistry Interleukin-10 Liver Liver Cirrhosis Male Matrix Metalloproteinase 2 Rats Rats, Sprague-dawley Tissue Inhibitor of Metalloproteinase-1 Tumor Necrosis Factor-alpha

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Effect of a Carbon Fibre-steel Fibre-graphite Conductive Filler on the Electrothermal Properties of Cementitious Materials

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Journal of Wuhan University of Technology(Materials Science) 2025年第1期40卷 141-151页

作者： FAN Yanan wei Hong zheng Hongyong DU Hongxiu Department of Architecture and Environmental Engineering Taiyuan UniversityTaiyuan 030032China College of Civil Engineering Taiyuan University of TechnologyTaiyuan 030032China

Carbon fibre,steel fibre and graphite were used as conductive fillers to prepare cementitious materials with excellent electrothermal *** electrically conductive cementitious materials with different volume dosages were analysed through compressive and flexural strength,electrochemical impedance spectroscopy and temperature rise *** equivalent circuit model was established to study the electrically conductive heat generation mechanism in the electrically conductive cementitious *** results indicate that the mechanical properties of cementitious composite materials with a ternary conductive phase are better than those of pristine cementitious materials because the fibrous filler improves their mechanical ***,the incorporation of graphite in the material reduces its *** fibrous and point-like conductive phase materials into the cementitious material enhances the overall conductive pathway and considerably reduces the electrical resistance of the cementitious material,enhancing its conductive *** volume ratios of carbon fibre,steel fibre and graphite that achieve an optimal complex doping in the cementitious material were 0.35%,0.6%and 6%,*** was determined using the mutation point of each circuit element parameter as the percolation *** addition,at a certain safety voltage,there is a uniform change between the internal and surface temperatures of the conductive cementitious material,and the heating effect in this materialis is considerably better than that in the pristine cementitious material.

关键词： cementitious composites conductive fillers electrothermal properties electrochemical impedance spectroscopy

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纤维支气管镜肺泡灌洗治疗支气管扩张合并感染的疗效

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中国内镜杂志 2015年第5期21卷 491-493页

作者：郑大炜河南省南阳市中心医院呼吸内科河南南阳473009

目的分析纤维支气管镜肺泡灌洗治疗支气管扩张合并感染的疗效及对血清TNF-α和hs-CRP含量的影响。方法选择在该院接受住院治疗的支气管扩张合并感染患者作为研究对象,随机分为接受常规治疗的对照组以及接受纤维支气管镜肺泡灌洗治疗的... 详细信息

目的分析纤维支气管镜肺泡灌洗治疗支气管扩张合并感染的疗效及对血清TNF-α和hs-CRP含量的影响。方法选择在该院接受住院治疗的支气管扩张合并感染患者作为研究对象,随机分为接受常规治疗的对照组以及接受纤维支气管镜肺泡灌洗治疗的观察组,比较两组患者的临床症状缓解时间,血清TNF-α和hs-CRP水平与血气水平等差异。结果观察组患者接受纤维支气管镜肺泡灌洗治疗后的发热、咳嗽咳痰及肺部湿啰音等症状体征缓解时间明显短于对照组患者(P<0.05)。观察组患者接受治疗后的血清TNF-α和hs-CRP水平值明显低于对照组患者(P<0.05)。观察组患者治疗后的Pa O2和Pa O2/Fi O2水平高于对照组;Pa CO2水平低于对照组患者(P<0.05)。结论纤维支气管镜肺泡灌洗治疗可以促进支气管扩张合并感染患者的早期康复,优化血清炎症因子水平,改善有效通气状态。

关键词：支气管扩张纤维支气管镜肺泡灌洗炎症因子水平

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AI-Enhanced Secure data Aggregation for Smart Grids with Privacy Preservation

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Computers, Materials & Continua 2025年第1期82卷 799-816页

作者： Congcong Wang Chen Wang Wenying zheng wei Gu School of Software Nanjing University of Information Science and TechnologyNanjing210044China School of Information Science and Engineering Zhejiang Sci-Tech UniversityHangzhou310018China State Key Laboratory of Public Big data Guizhou UniversityGuiyang550025China School of Computer Science and Technology(School of Artificial Intelligence) Zhejiang Sci-Tech UniversityHangzhou310018China School of Computer Science(School of Cyber Science and Engineering) Nanjing University of Information Science and TechnologyNanjing210044China

As smart grid technology rapidly advances,the vast amount of user data collected by smart meter presents significant challenges in data security and privacy *** research emphasizes data security and user privacy concerns within smart ***,existing methods struggle with efficiency and security when processing large-scale *** efficient data processing with stringent privacy protection during data aggregation in smart grids remains an urgent *** paper proposes an AI-based multi-type data aggregation method designed to enhance aggregation efficiency and security by standardizing and normalizing various data *** approach optimizes data preprocessing,integrates Long Short-Term Memory(LSTM)networks for handling time-series data,and employs homomorphic encryption to safeguard user *** also explores the application of Boneh Lynn Shacham(BLS)signatures for user *** proposed scheme’s efficiency,security,and privacy protection capabilities are validated through rigorous security proofs and experimental analysis.

关键词： Smart grid data security privacy protection artificial intelligence data aggregation

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Small modifications of endo-functionalized cavities lead to large changes in molecular interaction in water

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Science China Chemistry 2025年第1期68卷 369-376页

作者： Song-Meng Wang Hao Nian Yan-Fang Wang Li-Shuo zheng Yu-Tao zheng Yi-wei Dong Liping Huang Xiaoping Wang wei Jiang Liu-Pan Yang Department of Chemistry Southern University of Science and Technology School of Chemistry and Pharmaceutical Sciences State Key Laboratory for Chemistry and Molecular Engineering of Medicinal ResourcesGuangxi Normal University School of Pharmaceutical Science Hengyang Medical School University of South China

Studying molecular recognition in aqueous environments is crucial for understanding biological processes. Herein, we present a series of N-doped endo-functionalized anthracene-based cavities by introducing inward-directed pyridine units. The subtle modifications can significantly affect the binding properties of these receptors, which is similar to the mutations in biological systems. Combining theoretical calculations and molecular recognition experiments demonstrated that these “mutations” have an impact on the hydrophobicity and charge distribution of the hosts, consequently significantly influencing molecular *** is crucial for understanding the intricate mechanisms of molecular-level interactions in biological receptors.

关键词： host-guest interaction hydrophobic effect endo-functionalized cavity mutation pyridine

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