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Isobaric tags for relative and absolute quantitation-based quantitative proteomic analysis of X-linked inhibitor of apoptosis and H2AX in etoposide-induced renal cell carcinoma apoptosis

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Chinese Medical Journal 2019年第24期132卷 2941-2949页

作者： Tian-Shu Liu Chao Chen biao Zhou Bo-Wen xia Zong-Ping Chen Yong Yan Department of Urology Beijing Shijitan HospitalCapital Medical UniversityBeijing 100038China Department of Vascular Surgery People's Hospital of Peking UniversityBeijing 100044China Department of Urology The Affiliated Hospital of Zunyi Medical CollegeZunyiGuizhou 563000China

Background:X-linked inhibitor of apoptosis(xiaP)is a vital factor in the anti-apoptosis mechanism of tumors and is highly expressed in renal cell carcinoma(RCC).However,the mechanism through which xiaP regulates DNA damage repair is *** study investigated the regulatory mechanism of xiaP in etoposide-induced apoptosis in two Caki-1 cell lines with high or low xiaP ***:The two cell lines were established using RNA interference *** differentially expressed proteins in the two cell lines were globally analyzed through an isobaric tags for relative and absolute quantitation-based quantitative proteomics *** analysis revealed 255,375,362,and 5 differentially expressed proteins after 0,0.5,3,and 12 h of drug stimulation,respectively,between the two cell *** identified differentially expressed proteins were involved in numerous biological *** addition,the expression of histone proteins(H1.4,H2AX,H3.1,H3.2,and H3.3)was drastically altered,and the effects of xiaP silencing were accompanied by the marked downregulation of ***-protein interactions were assessed and confirmed through immunofluorescence and Western blot ***:The results suggested that xiaP may act as a vital cell signal regulator that regulates the expression of DNA repair-related proteins,such as H2AX,and influences the DNA repair ***:Given these functions,xiaP may be the decisive factor in determining the sensitivity of RCC cell apoptosis induction in response to chemotherapeutic agents.

关键词： Apoptosis H2AX iTRAQ Renal cell carcinoma X-linked inhibitor of apoptosis

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Expression and localization of Wolfram syndrome 1 gene in the developing rat pancreas

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World Journal of Gastroenterology 2009年第43期15卷 5425-5431页

作者： Rong Xu biao xia Jie Geng Jing Shi Hui Shi Li Yuan Wei De Department of Biochemistry and Molecular Biology Nanjing Medical University Nanjing 210029 Jiangsu Province China First School-of Clinical Medicine Nanjing Medical University Nanjing 210029 Jiangsu Province China

AIM： To investigate the expression and function of Wolfram syndrome 1 gene （WFS1） during the development of normal pancreas. METHODS： Pancreas from Sprague-dawley rat fetuses, embryos, young and adult animals were used in this study. Expression levels of WFS1 in pancreas of different development stages were detected by reverse transcription-polymerase chain reation （RT-PCR） and Western blotting. To identify the cell location of WFS1 in the developing rat pancreas, double-immunofluorescent staining was performed using antibodies to specific cell markers and WFS1, respectively. RESULTS： Compared to E15.5, the highest level of WFSl mRNA was detected at E18.5, the level of WFSl mRNA in E15.5 and P0 was less, and at a lowest at adult （P 〈 0.05 vs P0 and adult）, respectively. Compare to E15.5, the highest level of WFS1 was at P14 and lowest at P21 （P 〈 0.05 vs P14 and P21）, respectively. The WFSl positive staining is expressed in the normal developing rat pancreas mainly in the islet beta-cells and mesenchyme at each stage tested. CONCLUSION： These results indicate that WFSl may be involved in some aspects of pancreatic development and further research on WFS1 may provide new evidences to prove the interactions between mesenchyma and epithelia at the same time.

关键词： Wolfram syndrome 1 Pancreas Morphogenesis Mesenchyme Pancreatic beta cells

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基于跟踪微分器的离散滑模控制器

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控制与决策 2009年第10期24卷 1517-1521页

作者：孙彪孙秀霞空军工程大学工程学院西安710038

利用跟踪微分器设计了一种新的离散滑模控制器,可以从不连续的指令信号中合理提取连续信号及微分信号,且不需要利用线性外推的方法预测指令信号下一时刻的值及其微分.与基于趋近律的离散滑模控制器进行的对比仿真表明,所设计的控制器在... 详细信息

利用跟踪微分器设计了一种新的离散滑模控制器,可以从不连续的指令信号中合理提取连续信号及微分信号,且不需要利用线性外推的方法预测指令信号下一时刻的值及其微分.与基于趋近律的离散滑模控制器进行的对比仿真表明,所设计的控制器在保持传统滑模控制固有强鲁棒性的同时,控制器的输出几乎不存在抖振现象,而且在跟踪不连续的指令信号时,系统表现出良好的动态品质.

关键词：跟踪微分器离散滑模控制趋近律鲁棒性抖振

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The expression and prognostic value of protein tyrosine kinase 6 in early-stage cervical squamous cell cancer

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Chinese Journal of Cancer 2016年第7期35卷 374-381页

作者： xiao-Jing Wang Ying Xiong Ze-biao Ma Jian-Chuan xia Yan-Fang Li Sun Yat-Sen University Cancer Center State Key Laboratory of Oncology in South ChinaCollaborative Innovation Center of Cancer Medicine Guangzhou 510060Guangdong P.R.China Department of Gynecologic Oncology Sun Yat-Sen University Cancer CenterGuangzhou 510060GuangdongP.R. China Department of Biotherapy Sun Yat-Sen University Cancer CenterGuangzhou 510060GuangdongP.R. China

Background:Protein tyrosine kinase 6(PTK6) is overexpressed in many epithelial tumors and predicts poor ***,PTK6 expression status and its role in cervical squamous cell cancer are *** study aimed to investigate the expression level and clinical significance of PTK6 in early-stage cervical squamous cell ***:Quantitative reverse transcription-polymerase chain reaction(qRT-PCR) and western blotting analysis were performed to detect PTK6 mRNA and protein expression levels in 10 freshly frozen,early-stage cervical squamous cell cancer specimens and adjacent non-tumorous cervical *** expression of PTK6 was detected using immunohistochemical staining in 150 formalin-fixed,paraffin-embedded,early-stage cervical squamous cell cancer sections and 10 normal cervical tissue ***:The mRNA and protein levels of PTK6 in cancer tissues were higher than those in adjacent non-tumorous cervical *** analysis showed that PTK6 was not expressed in normal cervical tissues but was overexpressed in the cytoplasm of cervical squamous cell cancer *** level of PTK6 expression was significantly associated with tumor grade(P = 0.020).The 5-year overall survival rate of patients with high PTK6 expression was lower than that of patients with low PTK6 expression(81.3%vs.96.2%,P = 0.008).Multivariate Cox regression analysis showed that the expression level of PTK6 in cervical squamous cell cancer was an independent prognostic factor for patient survival(hazard ratio = 5.999,95%confidence interval 1.622-22.191,P< 0.05).Conclusions:PTK6 is overexpressed in cervical squamous ceil *** PTK6 expression is associated with reduced 5-year overall ***6 expression is an independent prognostic predictor for cervical cancer.

关键词：蛋白酪氨酸激酶鳞状细胞癌子宫颈预后早期反转录聚合酶链反应免疫组化染色组织切片

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离散系统最速控制综合函数

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控制与决策 2010年第3期25卷 473-477页

作者：孙彪孙秀霞空军工程大学工程学院西安710038

针对自抗扰控制技术中的fhan()函数,指出其只是离散系统最速控制综合函数的一种简化.详细推导了离散系统真正的最速控制综合函数——fsun(),完善了自抗扰控制技术的理论框架.理论分析和数值仿真表明,采用fsun()函数后的二阶离散系统,状... 详细信息

针对自抗扰控制技术中的fhan()函数,指出其只是离散系统最速控制综合函数的一种简化.详细推导了离散系统真正的最速控制综合函数——fsun(),完善了自抗扰控制技术的理论框架.理论分析和数值仿真表明,采用fsun()函数后的二阶离散系统,状态变量达到稳态时位置和速度曲线均不会出现超调,且达到稳态时所用的步数少于函数fhan().

关键词：最速控制综合函数离散系统抖振超调

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Research on Identification and Evaluation Technology and Management and Control System of Enterprise Major Risk Based on Preventing Serious Accidents

Research on Identification and Evaluation Technology and Man...

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作者： Wang xianhua xia Shuiguo Wang biao Sinosteel Wuhan Safety and Environmental Protection Research Institute

Some serious accidents happened in China in recent years show that,the management idea of taking the industry as the key to prevent serious accidents can not adapt to the current practice of safe production and how to set up a precise,forward-looking,systematic and comprehensive prevention and control system for preventing serious accidents has been a major task facing *** curb serious accidents,we must move forward the threshold,pre-control risks,close loop management and make continuous improvements and control risks before hidden dangers emerged and nip serious accidents in the *** systems are in motion,and the motion of the system is the result of contradictions and struggles in the *** is a universal principle in the universe,and the safety system obeys *** the safety system is complex in structure,it can be summarized as the result of the interaction between "risk" and "risk prevention and control".In this paper,curbing serious accidents and prevent major production safety risks as the goal,the system theory,cybernetics and information theory and risk management theory are used in production safety risk control,the accident risk point and risk unit are taken as objects,and the accident risk points higher risks(5 high-risks) identification as the foundation,to build a "1" + "5" index system of risk monitoring and control,set up serious risk assessment model,determine the serious risk determination threshold and grading standards,provide technical guidance for enterprises and governments to implement production safety risk control at different levels,and also provide technical support for the development of local production safety risk monitoring and warning *** realistic risk indicators"1"Unit real risk indicators are composed of risk severity aggregation indicators of risk points in the unit and risk control indicators of the *** severity aggregation indicators of risk points in the unit represent the severity of risk occu

关键词： safety production risk accident risk point risk assessment model significant risk determination threshold

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Dissemination of Antibiotic Resistance Genes Among Patients with Diarrhea — Freetown, Sierra Leone, 2018

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China CDC weekly 2022年第49期4卷 1093-1096页

作者： xia Chen Enmin Zhang Michael K Abdulai Alie Brima Tia Esther Debora Ngegba Jianhai Yin Songtao Xu Lili Wang xiaoping Dong Doris Harding biao Kan State Key Laboratory of Infectious Disease Prevention and Control National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing MunicipalityChina Sierra Leone-China Friendship Biological Safety Laboratory Chinese Center for Disease Control and PreventionFreetownSierra Leone and Beijing MunicipalityChina National Institute of Parasitic Diseases Chinese Center for Disease Control and PreventionChinese Center for Tropical Diseases ResearchShanghai MunicipalityChina National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijing MunicipalityChina Center for Global Public Health Chinese Center for Disease Control and PreventionBeijing MunicipalityChina Ministry of Health and Sanitation FreetownSierra Leone.

What is already known about this topic?Antibiotic resistance(AR)is a serious public health threat ***,the AR and antibiotic resistance genes(ARGs)data from West Africa,especially from Sierra Leone,are *** is added by this report?The study revealed ARGs’common dissemination,and multiplex antibiotic resistance genes in one *** blaNDM and blaOXA-48-like were first discovered in Sierra *** are the implications for public health practice?Basic information is provided for AR research and surveillance and highlights that effective AR surveillance among diarrhea patients is necessary for Sierra Leone and West Africa.

关键词： Antibiotic diarrhea Patients

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磁各向异性色散介质散射的Pad啨时域有限差分方法分析

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物理学报 2006年第4期55卷 1751-1758页

作者：杨利霞葛德彪西安电子科技大学理学院西安710071

根据矩阵Pad啨逼近理论,把磁化色散介质的相对磁导率张量表示成以jω为自变量的矩阵函数形式,用/t代替jω后过渡到时域,再引入离散时域移位算子代替时间微分算子.进而导出磁化色散介质中的磁感应强度B和磁场强度H在离散时域的色散关... 详细信息

根据矩阵Pad啨逼近理论,把磁化色散介质的相对磁导率张量表示成以jω为自变量的矩阵函数形式,用/t代替jω后过渡到时域,再引入离散时域移位算子代替时间微分算子.进而导出磁化色散介质中的磁感应强度B和磁场强度H在离散时域的色散关系,并将其具体应用于旋磁介质,得到了这种磁化色散介质的Pad啨时域有限差分方法的递推表达式.作为验证,用这种方法计算了磁化铁氧体球的后向雷达散射截面,所得结果与文献结果一致.理论推导及算例表明,该方法是正确和有效的.

关键词：各向异性介质色散介质矩阵Padé逼近时域有限差分方法

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Electro-red-luminescence from silicon oxide films embedded nm carbon particles and nm silicon particles

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兰州大学学报（自然科学版） 2005年第3期41卷 123-124页

作者： MAShu-yi CHENHai-xia ZHANGKai-biao SUNAi-min DepartmentofPhysics NorthwestNormalUniversityLanzhou730070China

Luminescence study from the silicon oxide filmsembedded nm carbon particles (CSO) and nm silicon particles (SSO) is of great importance in integrated optoelectronics. The pioneering work on electroluminescence (EL) fr... 详细信息

关键词：电镀红光氧化硅薄膜植入碳粒子硅粒子

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民乐县农民用水者协会运行实践与探讨

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节水灌溉 2006年第1期 53-54页

作者：夏彪甘肃省民乐县水务局甘肃民乐734500

农民用水者协会是实现农业灌溉民主管理和民主参与的一种有效形式。通过协会制度建设,明确工作职责,规范运行程序,提高管理水平,解决具体问题,开创了独具特色的运行模式,形成了公开、透明、广泛参与的民主决策机制和“总量控制,定额管理... 详细信息

农民用水者协会是实现农业灌溉民主管理和民主参与的一种有效形式。通过协会制度建设,明确工作职责,规范运行程序,提高管理水平,解决具体问题,开创了独具特色的运行模式,形成了公开、透明、广泛参与的民主决策机制和“总量控制,定额管理,明晰水权,配水到户,公众参与,水量交易”的运行管理机制。用水效益显著提高,协会运行模式和经验值得探讨和推广。

关键词：农村用水用水者协会民乐县

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