Isobaric tags for relative and absolute quantitation-based quantitative proteomic analysis of X-linked inhibitor of apoptosis and H2AX in etoposide-induced renal cell carcinoma apoptosis

作     者：Tian-Shu Liu Chao Chen Biao Zhou Bo-Wen Xia Zong-Ping Chen Yong Yan Liu Tian-Shu;Chen Chao;Zhou Biao;Xia Bo-Wen;Chen Zong-Ping;Yan Yong

作者机构：Department of UrologyBeijing Shijitan HospitalCapital Medical UniversityBeijing 100038China Department of Vascular SurgeryPeople's Hospital of Peking UniversityBeijing 100044China Department of UrologyThe Affiliated Hospital of Zunyi Medical CollegeZunyiGuizhou 563000China 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2019年第132卷第24期

页      面：2941-2949页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：This work was supported by grants from the National Natural Science Foundation of China(No.71432002) the Beijing Municipal Commission of Education,Science and Technology plan projects(No.KM 201310025017) 

主　　题：Apoptosis H2AX iTRAQ Renal cell carcinoma X-linked inhibitor of apoptosis 

摘      要：Background:X-linked inhibitor of apoptosis(XIAP)is a vital factor in the anti-apoptosis mechanism of tumors and is highly expressed in renal cell carcinoma(RCC).However,the mechanism through which XIAP regulates DNA damage repair is *** study investigated the regulatory mechanism of XIAP in etoposide-induced apoptosis in two Caki-1 cell lines with high or low XIAP ***:The two cell lines were established using RNA interference *** differentially expressed proteins in the two cell lines were globally analyzed through an isobaric tags for relative and absolute quantitation-based quantitative proteomics *** analysis revealed 255,375,362,and 5 differentially expressed proteins after 0,0.5,3,and 12 h of drug stimulation,respectively,between the two cell *** identified differentially expressed proteins were involved in numerous biological *** addition,the expression of histone proteins(H1.4,H2AX,H3.1,H3.2,and H3.3)was drastically altered,and the effects of XIAP silencing were accompanied by the marked downregulation of ***-protein interactions were assessed and confirmed through immunofluorescence and Western blot ***:The results suggested that XIAP may act as a vital cell signal regulator that regulates the expression of DNA repair-related proteins,such as H2AX,and influences the DNA repair ***:Given these functions,XIAP may be the decisive factor in determining the sensitivity of RCC cell apoptosis induction in response to chemotherapeutic agents.