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原发性血小板增多症患者中JAK2、CALR及三阴性驱动突变的临床特征比较

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中国实验血液学杂志 2024年第1期32卷 197-201页

作者：李雨蒙杨二鹏王子卿王德好牛继聪李芋锦明静孙明谦陈卓刘为易吕妍胡晓梅中国中医科学院研究生院北京100700 中国中医科学院西苑医院血液科北京100091 中国中医科学院西苑医院基础医学研究所北京100091

目的:探讨原发性血小板增多症(ET)患者的突变基因与临床特征的关系。方法:对2018年10月至2022年3月69例ET患者的临床资料进行回顾性分析。将患者按突变的驱动基因类型分为JAK2组、CALR组与三阴性组,对3组患者的性别、年龄、心血管危险... 详细信息

目的:探讨原发性血小板增多症(ET)患者的突变基因与临床特征的关系。方法:对2018年10月至2022年3月69例ET患者的临床资料进行回顾性分析。将患者按突变的驱动基因类型分为JAK2组、CALR组与三阴性组,对3组患者的性别、年龄、心血管危险因素、血栓、脾肿大、血常规、凝血状态进行分析。结果:69例ET患者中,46例伴随JAK2基因突变,14例伴随CALR基因突变,8例为三阴性,1例伴随MPL基因突变。3组患者的年龄、性别无显著差异(P>0.05)。JAK2组血栓率最高,为26.09%(12/46),三阴性组为12.5%(1/8),CALR组无血栓事件。JAK2组伴随脾肿大发生率最高,为34.78%(16/46),而三阴性组无脾肿大发生。JAK2组的白细胞数为(9.00±4.86)×10^(9)/L,显著高于CALR组的(6.03±2.32)×10^(9)/L(P<0.05)。JAK2组的血红蛋白含量为(148.43±18.79)g/L,显著高于三阴性组的(131.00±15.17)g/L(P<0.05)。JAK2组的红细胞压积为(0.44±0.06)%,亦显著高于三阴性组的(0.39±0.05)%(P<0.05)。JAK2组血小板数为(584.17±175.77)×10^(9)/L,显著低于CALR组的(703.07±225.60)×10^(9)/L(P<0.05)。JAK2组和三阴性组纤维蛋白原含量分别为(2.64±0.69)g/L和(3.05±0.77)g/L,均显著高于CALR组的(2.24±0.47)g/L(P<0.05,P<0.01)。三阴性组活化部分凝血活酶时间为(28.61±1.99)s,较CALR组的(31.45±3.35)s显著降低(P<0.05)。结论:不同驱动基因突变ET患者的血细胞计数与凝血状态存在差异。与CALR组相比,JAK2组血栓率、白细胞数和纤维蛋白原含量均显著升高,而血小板数显著降低;与三阴性组相比,JAK2组脾大发生率和红细胞压积显著升高;与CALR组相比,三阴性组的纤维蛋白原含量显著升高、活化部分凝血活酶时间显著降低。

关键词：原发性血小板增多症二代基因测序驱动突变凝血

A novel mutation in ACS11 leads to androecy in cucumber

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Journal of Integrative Agriculture 2023年第11期22卷 3312-3320页

作者： wang Jie li Shuai chen chen ZHANG Qi-qi ZHANG Hui-min CUI qing-zhi CAI Guang-hua ZHANG xiao-peng CHAI Sen WAN li yang Xue-yong ZHANG Zhong-hua HUANG San-wen chen Hui-ming SUN Jin-jing College of Horticulture Qingdao Agricultural UniversityQingdao 266109P.R.China State Key Laboratory of Vegetable Biobreeding Institute of Vegetables and FlowersChinese Academy of Agricultural SciencesBeijing 100081P.R.China Hunan Vegetable Research Institute Hunan Academy of Agricultural SciencesChangsha 410125P.R.China Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs Agricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518124P.R.China chinese Academy of Tropical Agriculture Sciences Haikou 571101P.R.China College of Horticulture Hunan Agricultural UniversityChangsha 410128P.R.China

Sex determination in plants gives rise to unisexual flowers. A better understanding of the regulatory mechanism underlying the production of unisexual flowers will help to clarify the process of sex determination in plants and allow researchers and farmers to harness heterosis. Androecious cucumber(Cucumis sativus L.) plants can be used as the male parent when planted alongside a gynoecious line to produce heterozygous seeds, thus reducing the cost of seed production. The isolation and characterization of additional androecious genotypes in varied backgrounds will increase the pool of available germplasm for breeding. Here, we discovered an androecious mutant in a previously generated ethyl methanesulfonate(EMS)-mutagenized library of the cucumber inbred line ‘406’. Genetic analysis, whole-genome resequencing, and molecular marker-assisted verification demonstrated that a nonsynonymous mutation in the ethylene biosynthetic gene 1-AMINOCYCLOPROpanE-1-CARBOXYLATE SYNTHASE 11(ACS11) conferred androecy. The mutation caused an amino acid change from serine(Ser) to phenylalanine(Phe) at position 301(S301F). In vitro enzyme activity assays revealed that this S301F mutation leads to a complete loss of enzymatic activity. This study provides a new germplasm for use in cucumber breeding as the androecious male parent, and it offers new insights into the catalytic mechanism of ACS enzymes.

关键词： cucumber androecy ethylene marker-assisted breeding

An alphavirus replicon particle delivering prefusion-stabilized spike protein provides potent immunoprotection against SARS-CoV-2 Omicron variant

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Signal Transduction and Targeted Therapy 2023年第1期8卷 6-9页

作者： Hong-qing Zhang Ya-Nan Zhang Zhe-rui Zhang xiao-ling chen Yan-Yan Hu Yu-Jia Shi jing wang cheng-lin Deng Bo Zhang xiao-dan li Han-qing Ye Key Laboratory of Special Pathogens and Biosafety Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhanChina University of chinese Academy of Sciences Beijing 100049China Hunan Normal University School of MedicineChangsha 410081China

Dear Editor,The prolonged outbreak and spread of coronavirus disease 2019(COVID-19),caused by SARS-CoV-2 poses a great threat to global economic and public *** protective efficacy of the vaccines based on the spike pr... 详细信息

关键词： protective stabilized particle

A novel mechanism fusion data control method for slab camber in hot rolling

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Journal of Iron and Steel Research International 2023年第5期30卷 960-970页

作者： Le-bao Song Dong Xu Peng-fei liu Jin-hang Zhou Hui-qing Yan jing-dong li Hai-nan He Hai-jun Yu xiao-chen wang Quan yang National Engineering Technology Research Center of Flat Rolling Equipment University of Science and Technology BeijingBeijing 100083China Xinjiang Joinworld Co. Ltd.Urumqi 830000XinjiangChina 2250 Hot Strip Mill Hunan Lianyuan Iron and Steel Co.Ltd.Loudi 417000HunanChina

For asymmetric plate shape,control over the hot rolling process mainly depends on the subjective judgement and personal experience of the operator as there are great deviations and much instability in hot ***,the intrinsic mechanisms and sensitivity affecting characteristic parameters and variables in the asymmetric rolling process remain ***,a novel mechanism fusion data control method for slab camber in hot rolling using dimensional analysis and data-driven technique was *** approach of dimensional analysis was used to establish a mathematical model and analyse the main parameters affecting the slab camber of the rough ***,the established mathematical model combined with the data-driven techniques was employed to accurately predict the slab bending ***,the superiority and effectiveness of the proposed model were demonstrated by a comparison with three regression ***,the proposed control strategy was successfully applied in a 1580 mm hot rolling industrial *** automatic control results show that the hit rate of slab cambers in different sizes from 10 to 30 mm is improved,and the quality stability of intermediate slab is significantly improved.

关键词： Mechanism model Dimensional analysis data-driven technique Camber control Hot rolling

Distribution and isotopic signature of 2-alkyl-1,3,4-trimethylbenzenes in the Lower Paleozoic source rocks and oils of Tarim Basin:Implications for the oil-source correlation

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Petroleum Science 2022年第6期19卷 2572-2582页

作者： Tao-Hua He Wen-Hao li Shuang-Fang Lu Er-Qiang yang Tao-Tao jing Jun-Feng Ying Peng-Fei Zhu Xiu-Zhe wang Wen-qing pan Zhong-Hong chen Key Laboratory of Oil and Gas Resources and Exploration Technology Yangtze UniversityWuhanHubei430100China School of Geosciences China University of Petroleum(East China)QingdaoShandong266580China Key Laboratory of Deep Oil and Gas China University of Petroleum(East China)QingdaoShandong266580China Sanya Offshore Oil&Gas Research Institute Northeast Petroleum UniversitySanyaHainan 572025China Petroleum Exploration&Production Research Institute Tarim Oilfield CompanyPetro ChinaKorlaXinjiang841000China

Series of 2-alkyl-1,3,4-trimethylbenzenes(ATMBs)were detected in most of crude oils and source rocks collected from various strata and locations of the Tarim *** appeared to have heavy carbon isotopic signatures(δ13C,up to~-16‰)compared to those hydrocarbons from oxygenic phototrophic organisms,indicating that the unequivocal source of green sulfur bacteria(GSB)and photic zone euxinia(PZE)existed in the original *** the high paleoproductivity,the PZE may have enhanced the preservation of organic matter,which triggered the formation of extremely organic-rich source rocks with TOC up to 29.8%for the Lower Cambrian Yuertus Formation(€1y).The coexistence of ATMBs and the diagnostic products from secondary alterations(e.g.,abundant 25-norhopanes,thiadiamondoids,and diamondoids)indicated a stronger ability of *** with the results of quantitatively de-convoluting mixed oil,the oil-source correlation based on ATMBs from a large number of Lower Paleozoic samples of the Tarim Basin suggested that the abundant deep crude oil resources co ntained a dominant contribution(>50%)from the€1y source ***,the ATMBs,as diagnostic biomarkers indicating unequivocal precursors under special habitat conditions,might provide important insights for the exploration of deep Lower Paleozoic crude oils in the Tarim Basin.

关键词： 2-alkyl-1,3,4-trimethylbenzenes Carbon isotopes Deep lower paleozoic crude oils Thiadiamondoid Oil-source correlation Tarim Basin

Single-Walled Carbon Nanotube Networked Field-Effect Transistors Functionalized with Thiolated Heme for NO_(2) Sensing

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chinese Physics Letters 2011年第12期28卷 249-251页

作者： WEI Ang li Wei-Wei wang jing-Xia LONG qing wang Zhao XIONG li DONG xiao-chen HUANG Wei Key Laboratory for Organic Electronics and Information Displays and Institute of Advanced MaterialsNanjing University of Posts and TelecommunicationsNanjing 210046

The gas sensing properties of the single-walled carbon nanotube networked field-effect transistors for NO_(2) are *** the modification of the gold contact electrodes of the carbon nanotube transistors with the thiolated heme,the NO_(2) sensing results indicate that the sensing sensitivity of the modified transistors is enhanced greatly and the sensing limit can reach below 100 *** is also proposed that the mechanism of the sensitivity enhancement for NO_(2) detection mainly results from the modulation of the Schottky energy barrier at the Au/CNTs junction upon thiolated heme facilitated NO_(2) adsorption.

关键词： limit Carbon mechanism

Bis(2-butoxyethyl) Phthalate Delays Puberty Onset by Increasing Oxidative Stress and Apoptosis in Leydig Cells in Rats

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Biomedical and Environmental Sciences 2023年第1期36卷 60-75页

作者： liU Miao qing chen Hai Qiong daI Hai Peng li jing jing TIAN Fu Hong wang Yi Yan chen Cong De li xiao Heng li Jun Wei li Zhong Rong GE Ren Shan Department of Pediatric Surgery the Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical UniversityWenzhou 325027ZhejiangChina Key Laboratory of Structural Malformations in children of Zhejiang Province Wenzhou 325000ZhejiangChina

Objective This study investigated the effects of bis(2-butoxyethyl) phthalate(BBOP) on the onset of male puberty by affecting Leydig cell development in *** Thirty 35-day-old male Sprague-dawley rats were randomly allocated to five groups mg/kg bw per day that were gavaged for 21 days with BBOP at 0, 10, 100, 250, or 500 mg/kg bw per day. The hormone profiles;Leydig cell morphological metrics;mRNA and protein levels;oxidative stress;and AKT,mTOR, ERK1/2, and GSK3β pathways were *** BBOP at 250 and/or 500 mg/kg bw per day decreased serum testosterone, luteinizing hormone, and follicle-stimulating hormone levels mg/kg bw per day(P day decreased Leydig cell number mg/kg bw per day and downregulated Cyp11a1, Insl3, Hsd11b1,and Dhh in the testes, and Lhb and Fshb mRNAs in the pituitary gland(P day(P day decreased AKT1/AKT2, mTOR, and ERK1/2 phosphorylation, and GSK3β and SIRT1levels mg/kg bw per day(P dative stress and apoptosis in Leydig cells in *** graphical abstract is available on the website ***.

关键词： Bis(2-butoxyethyl)phthalate Leydig cells Testosterone Oxidative stress Gonadotropin

Downregulation of SL-ZH13 transcription factor gene expression decreases drought tolerance of tomato

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Journal of Integrative Agriculture 2019年第7期18卷 1579-1586页

作者： ZHAO Ting-ting wang Zi-yu BAO Yu-fang ZHANG xiao-chun yang Huan-huan ZHANG Dong-ye JIANG jing-bin ZHANG He li jing-fu chen qing-shan XU Xiang-yang Laboratory of Genetic Breeding in Tomato College of Horticulture and Landscape ArchitectureNortheast Agricultural UniversityHarbin 150030P.R. China College of Agronomy Northeast Agricultural UniversityHarbin 150030P.R. China

Zinc finger-homeodomain proteins(ZF-HDs) are transcription factors that regulate plant growth,development,and abiotic stress *** SL-ZH13 gene was found to be significantly upregulated under drought stress treatment in tomato(Solanum lycopersicum) leaves in our previous *** this study,to further understand the role that the SL-ZH13 gene plays in the response of tomato plants to drought stress,the virus-induced gene silencing(VIGS) method was applied to downregulate SL-ZH13 expression in tomato plants,and these plants were treated with drought stress to analyze the changes in drought *** SL-ZH13 silencing efficiency was confirmed by quantitative real-time PCR(qRT-PCR) *** SL-ZH13-silenced plants,the stems wilted faster,leaf shrinkage was more severe than in control plants under the same drought stress treatment conditions,and the mean stem bending angle of SL-ZH13-silenced plants was smaller than that of control *** analyses showed that the activity of superoxide dismutase(SOD) and peroxidase(POD) and the content of proline(Pro) in SL-ZH13-silenced plants were lower than those in control plants after 1.5 and 3 h of drought stress *** malondialdehyde(Mda) content in SL-ZH13-silenced plants was higher than that in control plants after 1.5 and 3 h of drought stress treatment,and H2O2 and O2^-· accumulated much more in the leaves of SL-ZH13-silenced plants than in the leaves of control *** results suggested that silencing the SL-ZH13 gene affected the response of tomato plants to drought stress and decreased the drought tolerance of tomato plants.

关键词： SL-ZH13 gene VIGS drought stress tomato transcription factor

Trends and Prospects of Stem Cell Research in china

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chinese Medical Sciences Journal 2016年第2期31卷 116-120页

作者： jing-wen Cao Lu Zhang Ye li qing yang Wen-hua Fu xiao-chen wang Wen-long Huang Shool of International Pharmaceutical Business China Pharmaceutical University Office of Science and Technology Management Chinese Academy of Medical Sciences & Peking Union Medical College State Key Laboratory of Experimental Hematology Institute of Hematology and Blood Diseases Hospital Chinese Academy of Medical Sciences & Peking Union Medical College Department of Science and Technology Management National Health and Family Planning Commission of the People's Republic of China

Great progresses have been made in fundamental and clinical stem cell research in china in recent years. The official policy on stem cells, which was announced in 2015, seems as the spring of stem cell therapy in china. However, the regulation, governance, and management of clinical expectations are still challenging. This review summarized the current stem cell research and development in the field, as well as its rapidly evolving commercial, regulatory and ethical environment in china. As expected, the prospects of stem cells in china look prospective.

关键词： stem cell policy regulations china