A novel mutation in ACS11 leads to androecy in cucumber

作     者：WANG Jie LI Shuai CHEN Chen ZHANG Qi-qi ZHANG Hui-min CUI Qing-zhi CAI Guang-hua ZHANG Xiao-peng CHAI Sen WAN Li YANG Xue-yong ZHANG Zhong-hua HUANG San-wen CHEN Hui-ming SUN Jin-jing WANG Jie;LI Shuai;CHEN Chen;ZHANG Qi-qi;ZHANG Hui-min;CUI Qing-zhi;CAI Guang-hua;ZHANG Xiao-peng;CHAI Sen;WAN Li;YANG Xue-yong;ZHANG Zhong-hua;HUANG San-wen;CHEN Hui-ming;SUN Jin-jing

作者机构：College of HorticultureQingdao Agricultural UniversityQingdao 266109P.R.China State Key Laboratory of Vegetable BiobreedingInstitute of Vegetables and FlowersChinese Academy of Agricultural SciencesBeijing 100081P.R.China Hunan Vegetable Research InstituteHunan Academy of Agricultural SciencesChangsha 410125P.R.China Genome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518124P.R.China Chinese Academy of Tropical Agriculture SciencesHaikou 571101P.R.China College of HorticultureHunan Agricultural UniversityChangsha 410128P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2023年第22卷第11期

页      面：3312-3320页

核心收录：

学科分类：09[农学] 0902[农学-园艺学] 090202[农学-蔬菜学] 

基　　金：supported by the National Key Research and Development Program of China (2018YFD1000803) the National Natural Science Foundation of China (31701933 and 32002036) the Shandong Provincial Natural Science Foundation, China (ZR2020QC157) 

主　　题：cucumber androecy ethylene marker-assisted breeding 

摘      要：Sex determination in plants gives rise to unisexual flowers. A better understanding of the regulatory mechanism underlying the production of unisexual flowers will help to clarify the process of sex determination in plants and allow researchers and farmers to harness heterosis. Androecious cucumber(Cucumis sativus L.) plants can be used as the male parent when planted alongside a gynoecious line to produce heterozygous seeds, thus reducing the cost of seed production. The isolation and characterization of additional androecious genotypes in varied backgrounds will increase the pool of available germplasm for breeding. Here, we discovered an androecious mutant in a previously generated ethyl methanesulfonate(EMS)-mutagenized library of the cucumber inbred line ‘406’. Genetic analysis, whole-genome resequencing, and molecular marker-assisted verification demonstrated that a nonsynonymous mutation in the ethylene biosynthetic gene 1-AMINOCYCLOPROPANE-1-CARBOXYLATE SYNTHASE 11(ACS11) conferred androecy. The mutation caused an amino acid change from serine(Ser) to phenylalanine(Phe) at position 301(S301F). In vitro enzyme activity assays revealed that this S301F mutation leads to a complete loss of enzymatic activity. This study provides a new germplasm for use in cucumber breeding as the androecious male parent, and it offers new insights into the catalytic mechanism of ACS enzymes.