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Assessing validation of dual fluoroscopic image matching method for measurement of in vivo spine kinematics

Chinese Medical Journal 2011年第11期124卷 1689-1694页

作者： BAI Jian-qiang HU Yong-cheng duli-qing HE Jing-liang LIU Kai LIU Zhongjun XIA Qun Department of Orthopaedics Peking University Third HospitalBeijing 100191 China Department of Spinal Surgery Tianjin Hospital Tianjin 300211China Institute of Radiation Medicine Chinese Academy of MedicalSciences & Peking Union Medical College Tianjin KeyLaboratory of Molecular Nuclear Medicine Tianjin 300192 China

Background Accurate knowledge of the spinal structural functions is critical to understand the biomechanical factors that affect spinal pathology. Many studies have investigated the human vertebral motion both in vitro and in vivo. However, determination of in vivo motion of the vertebrae under physiologic loading conditions remains a challenge in biomedical engineering because of the limitations of current technology and the complicated anatomy of the spine. Methods For in vitro validation, a human lumbar specimen was imbedded with steel beads and moved to a known distance by an universal testing machine （UTM）.

关键词： lumbar spine validation dual fluoroscopes matching method

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Isolation and oral immunogenicity assessment of porcine epidemic diarrhea virus NH-TA2020 strain:One of the predominant strains circulating in China from 2017 to 2021

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Virologica Sinica 2022年第5期37卷 646-655页

作者： Xiaowen Li Yang Li Jiapei Huang Yali Yao Wenying Zhao Yunjing Zhang Jie qing Jing Ren Zhong Yan Zewei Wang Xiaofang Hu duli Kang Hongqiang Liu Zhichun Yan New Hope Liuhe Co. Ltd.Chengdu610041China National Research Center for Veterinary Medicine Luoyang471000China Shandong New Hope Liuhe Agriculture and Animal Husbandry Technology Co. Ltd.(NHLH Academy of Swine Research)Dezhou253034China Swine Health Data and Intelligent Monitoring Project Laboratory Dezhou UniversityDezhou253011China Pulike Biological Engineering Inc. Luoyang471000China

Porcine epidemic diarrhea(PED)caused by porcine epidemic diarrhea virus(PEDV)is one of the most devastating diseases in the global pig industry due to its high mortality rate in *** vaccines can effectively enhance the gut-mammary gland-secretory IgA axis to boost lactogenic immunity and passive protection of nursing piglets against PEDV *** 2017 to 2021,we collected 882 diarrhea samples from 303 farms in China to investigate the epidemiology of *** result showed that about 52.15%(158/303)of the farms were positive for PEDV with an overall detection rate of 63.95%(564/882)of the *** S1 fragments of S gene from 104 strains were sequenced for the phylogenetic analysis.A total of 71 PEDV strains(68.27%)sequenced in this study were clustered into the predominant G2c subgroup,while the newly-defined G2d strains(9.62%)were identified in three provinces of *** NH-TA2020 strain of G2c subgroup was isolated and cultured,and its infection to piglets caused watery diarrhea within 24 h,indicating its strong *** administration of NH-TA2020 strain to pregnant gilts stimulated high levels of IgA antibody in *** piglets fed by the gilts above were challenged with NH-TA2020 strain or CH-HeB-RY-2020 strain from G2d subgroup,and the clinical symptoms and virus shedding were significantly reduced compared to the mock *** findings suggest that G2c subgroup is the predominant branch circulating in China from 2017 to *** administration of NH-TA2020 enhances maternal IgA and lactogenic immune responses,which confer protection against the homologous and emerging G2d PEDV strains challenges in neonates.

关键词： Porcine epidemic diarrhea virus(PEDV) Molecular epidemiological investigation Virus isolation Lactogenic immune IgA

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