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生命科学世纪呼唤酶工程教学改革深入发展

河北农业大学学报（农林教育版） 2009年第3期11卷 326-328页

作者：窦世娟侯春燕侯名语魏凤菊刘刚河北农业大学生命科学学院河北保定071001

酶工程的发展是迅猛的,无论在理论上和应用上都取得了很大的革命性成效。《酶工程》是高校生物技术专业的重要专业课之一,如何使酶工程的教学跟上国际步伐、符合国内新需求,培养优秀的技术人才,是目前《酶工程》教学环节急需解决的问题... 详细信息

酶工程的发展是迅猛的,无论在理论上和应用上都取得了很大的革命性成效。《酶工程》是高校生物技术专业的重要专业课之一,如何使酶工程的教学跟上国际步伐、符合国内新需求,培养优秀的技术人才,是目前《酶工程》教学环节急需解决的问题。结合工作实践,从合理选择教材、优化教学内容、讨论式教学、强化实验课建设等多方面进行了深入探讨。

关键词：酶工程教学改革讨论式教学实验课建设

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赞皇大枣采后成熟衰老期生理生化变化

河北农业科学 2004年第2期8卷 13-16页

作者：及华关军锋窦世娟安永芳孙玉龙河北省农林科学院遗传生理研究所河北石家庄050051

以 2种成熟度 (全红和半红 )的赞皇大枣为试材 ,研究了采后常温下贮藏时果实的生理生化变化。结果表明 ,果实的褐变度、SOD活性、MDA和糖的含量随贮藏期的延长而增加 ,与半红果相比 ,全红果的褐变度、MDA和糖的含量较高 ,SOD活性差异较... 详细信息

以 2种成熟度 (全红和半红 )的赞皇大枣为试材 ,研究了采后常温下贮藏时果实的生理生化变化。结果表明 ,果实的褐变度、SOD活性、MDA和糖的含量随贮藏期的延长而增加 ,与半红果相比 ,全红果的褐变度、MDA和糖的含量较高 ,SOD活性差异较小 ;贮藏前期 ,全红果的POD和PPO活性、蛋白质和总酚含量高于半红果 ,而在贮藏后期又低于半红果。

关键词：赞皇大枣采后成熟衰老期生理变化生化变化贮藏保鲜技术

Method for maneuvering target video frequency tracking based on inductive factor of posture information

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Journal of Systems Engineering and Electronics 2010年第2期21卷 261-267页

作者： Xun Chen Lihua dou juan Zhang Department of Automatic Control School of Information Science and Technology Beijing Institute of Technology Beijing 100081 P. R. China

A method for maneuvering target tracking based on in- ductive factor of posture information is proposed. A distinguished charactedstic of video frequency tracking is that it can capture the target posture changes from its picture easily, and the posture change means the motive model of the target will change. This information is very important to predict the trace of maneuvering target. Based on this idea, the quantified values of the target pos- ture change are obtained using Hough algorithm, this key values are defined as inductive factor of posture information, and then, the multiple grey trace predict models are established and the degrees of fuzzy subordinate values for every model are calculated with the inductive factor, the maneuvering extent values are determined by a new analysis method of stochastic differential equations for each model used to modify the degree of fuzzy subordinate values, these constitute the weighted values for every grey predict collec- tion. Finally, the synthesis predicting weighted result is obtained. The experimental results show that the new method is superior to the conventional algorithm.

关键词： target posture grey prediction hough algodthm de- gree of fuzzy subordinate.

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我国微藻生物柴油的研究背景与发展战略

生物技术进展 2015年第2期5卷 85-88页

作者：吴立柱窦世娟从均广杨国军刘建凤河北农业大学生命科学学院河北保定071000 河北公安警察职业学院石家庄050091 河北省唐山市丰润区农业畜牧水产局河北唐山063000 河北大学生命科学学院河北保定071002

生物柴油是可再生能源开发利用的重要发展方向。藻类制备生物柴油具有产油量高、生长速度快、环境适应能力强、不与农作物争夺农田和淡水资源等优势。从微藻制备生物柴油着手,简要介绍了生物柴油的生产原料与发展历程、微藻油脂的组成... 详细信息

生物柴油是可再生能源开发利用的重要发展方向。藻类制备生物柴油具有产油量高、生长速度快、环境适应能力强、不与农作物争夺农田和淡水资源等优势。从微藻制备生物柴油着手,简要介绍了生物柴油的生产原料与发展历程、微藻油脂的组成与生物合成途径、微藻制备柴油的工艺与瓶颈及解决策略,最后对微藻制备生物柴油技术提出了近中远期发展目标及展望。

关键词：生物柴油可再生能源微藻发展战略

过表达OsMPK17激酶蛋白质增强了水稻的耐旱性

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作物学报 2020年第1期46卷 20-30页

作者：马金姣兰金苹张彤陈悦郭亚璐刘玉晴燕高伟魏健窦世娟杨明李莉云刘国振河北农业大学生命科学学院河北保定071001 河北北方学院生命科学研究中心河北张家口075000 中国农业科学院农业基因组研究所广东深圳518116

促分裂原活化蛋白质激酶(mitogen-activated protein kinase, MAPK)在真核生物中高度保守,在水稻逆境应答反应中也发挥着重要作用。本研究表达纯化了水稻OsMPK17蛋白质,制备了特异性抗体,对多种非生物逆境胁迫下的蛋白质样品进行免疫印... 详细信息

促分裂原活化蛋白质激酶(mitogen-activated protein kinase, MAPK)在真核生物中高度保守,在水稻逆境应答反应中也发挥着重要作用。本研究表达纯化了水稻OsMPK17蛋白质,制备了特异性抗体,对多种非生物逆境胁迫下的蛋白质样品进行免疫印迹分析,发现OsMPK17蛋白质在干旱胁迫下诱导表达,提示该蛋白质在干旱胁迫应答中发挥作用。对脱落酸和茉莉酸甲酯处理的离体叶片蛋白质分析发现,OsMPK17蛋白质表达丰度下降,提示该蛋白质的功能发挥可能受激素调控。为此,构建了过表达OsMPK17蛋白质的载体,转化水稻后筛选获得了OsMPK17蛋白质过表达的纯合株系。田间种植鉴定结果表明,转基因株系的株高变矮、穗长变短、结实率降低。种子萌发期拟旱(PEG-6000)处理条件下,过表达OsMPK17株系的种子长势明显比野生型好,根长与芽长均显著大于野生型。幼苗期失水试验表明,转基因植株的失水率低于野生型。在土培干旱胁迫后恢复浇水的试验中,过表达OsMPK17蛋白质的转基因水稻生长也好于野生型。综上,过表达OsMPK17蛋白质提高了水稻的耐旱性。本研究增进了对水稻OsMPK17基因功能的了解。

关键词：水稻 MAPK蛋白质免疫印迹逆境胁迫过表达基于抗体的蛋白质组学

Net zero emission goals demand worldwide cooperation

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National Science Review 2023年第11期10卷 93-95页

作者： shi Xue dou Wei-Hong Lai Chris Cook Institute of Energy Materials Science University of Shanghai for Science and Technology Institute for Superconducting and Electronic Materials University of Wollongong Faculty of Engineering and Information Sciences University of Wollongong

The serious threat of climate change has become a reality of daily life all over the world. There is no priority facing humans at present more urgent than climate change. The goal of achieving net zero emissions (NZEs) within a time frame short enough to avoid catastrophic changes can only be accomplished through worldwide cooperation.

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Research on Cognitive Cooperation in Multi-Agent Systems

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Wuhan University Journal of Natural Sciences 2006年第1期11卷 278-282页

作者： SUN juan dou Wan-chun State Key Laboratory for Novel Software Technology Nanjing University Nanjing 210093 JiangsuChina Department of Computer Science and Technology Nanjing University Nanjing 210093Jiangsu China

The accomplishment of a complex problem usually involves cooperation between participators with different knowledge background concerned. This paper identifies interdependency between different sub problems （through problem decomposition） as the major factor that influences cooperative relations in multi-Agent systems, based on which we propose an efficient means to measure cooperation coefficient （degree） between different Agents. Then cognitive cooperation between Agents is analyzed which aims at collecting the wisdom of the cognitive community for a systematic solution to the overall problem.

关键词： multi-Agent systems cognitive cooperation knowledge flow

Effect of advanced paternal age on reproductive outcomes in IVF cycles of non-male-factor infertility: a retrospective cohort study

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Asian Journal of Andrology 2023年第2期25卷 245-251页

作者： Xin-Mei Lu Yu-Bing Liu dou-dou Zhang Xiang Cao Tian-Cheng Zhang Miao Liu Hui-juan shi Xi Dong Su-Ying Liu Reproductive Medicine Center Zhongshan HospitalFudan UniversityNo.180 Fenglin RoadShanghai 200032China NHC Key Lab of Reproduction Regulation Shanghai Institute for Biomedical and Pharmaceutical TechnologiesPharmacy SchoolFudan UniversityNo.2140 Xietu RoadShanghai 200032China Center for Reproductive Medicine Tenth People’s Hospital of Tongji University301 Middle Yanchang RoadShanghai 200072China

Advanced paternal age has been overlooked,and its effect on fertility remains *** studies have focused mainly on intracytoplasmic sperm injection(ICSI)cycles in men with ***,few studies have reported on men with semen parameters within reference ***,we conducted a retrospective cohort study analyzing the reproductive outcomes of couples with non-male-factor infertility undergoing in vitro fertilization(IVF)*** total,381 cycles included were subgrouped according to paternal age(0.05).The pregnancy rate was not significantly different in the 35–39-year-old group(42.0%;P>0.05),but was significantly lower in the≥40-year-old group(26.1%;P0.05),but showed a declining *** miscarriage rate significantly increased in the 35–39-year-old group(44.8%)compared with that in the<35-year-old group(21.0%;P<0.05).No abnormality in newborn birth weight was *** results indicated that paternal age over 40 years is a key risk factor that influences the assisted reproductive technology success rate even with good semen parameters,although it has no impact on embryo development.

关键词： embryo development implantation in vitro fertilization paternal age pregnancy sperm

Synthesis and Crystal Structure of 1,3-Trans-1,4-cis- 3,4-di(4-bromophenyl)-1',2',3'-trihydro-1-hydroxy- benzo[g]bicyclo[3.3]octane-2-spiro-2'-indene-1'-one

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Chinese Journal of Structural Chemistry 2006年第2期25卷 187-191页

作者： shi Da-Qing LI Zheng-Yi shi Chun-Ling dou Guo-Lan Department.of Chemistry Xuzhou Normal University Xuzhou Jiangsu 221116 China Key Laboratory of Biotechnology on Medical Plants of Jiangsu Province Xuzhou 221116 China

The title compound C35H30Br2O3 （C32H24Br2O2·CH3COCH3） has been synthesized by the dimerization of 2-（4＇-bromobenzal）-1-indenone induced by low-valent titanium reagent, and characterized by elemental analysis, IR,^1H NMR and X-ray single-crystal diffraction. The crystal belongs to triclinic, space group P1^- with a = 10.273（7）, b = 11.036（7）, c = 13.944（9） ,A°, a = 73.972（9）, β =76.577（10）,γ= 88.871（10）°, V = 1476.3（17）A°^3, Mr = 658.41, Z = 2, Dr = 1.481 g/cm^3, μ（MoKa） = 2.780 mm^-1, F（000） = 668, the final R = 0.0452 and wR = 0.0975. X-ray analysis reveals that the two cyclopentane rings spiro-fused each other adopt envelope conformations, while the other cyclopentane ring （C（1）--C（2）-C（7）-C（9）） is coplanar. In addition, there is a classical intermolecular hydrogen bond O（1）-H（1）...O（2） connecting the adjacent molecules into dimers.

关键词： benzo[g]bicyclo[3.3]octane-2-spiro-2'-indene-1'-one synthesis crystal structure,low-valent titanium