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Effects of ultrasonic-assisted extraction on bioactive compounds,volatile flavors and antioxidant activities of vine tea water extracts

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Traditional Medicine Research 2025年第1期10卷 73-81页

作者： xiao-long Zhou Wei-Jin Jiang Ji yu Mao-Jun Yao yun-Tong Li Hunan Provincial Key Laboratory of Research Resource Mining and High-valued Utilization on Edible&Medicinal PlantCollege of Chemistry and Chemical EngineeringJishou UniversityJishou 416000China Hunan Gaoge Dairy Co. Ltd.Changsha 410000China

Background:Ampelopsis grossedentata,vine tea,which is the tea alternative beverages in *** vine tea processing,a large amount of broken tea is produced,which has low commercial ***:This study investigates the influence of different extraction methods(room temperature water extraction,boiling water extraction,ultrasonic-assisted room temperature water extraction,and ultrasonic-assisted boiling water extraction,referred to as room temperature water extraction(RE),boiling water extraction(BE),ultrasonic assistance at room temperature water extraction(URE),and ultrasonic assistance in boiling water extraction(UBE))on the yield,dihydromyricetin(DMY)content,free amino acid composition,volatile aroma components,and antioxidant properties of vine tea ***:A notable influence of extraction temperature on the yield of vine tea extracts(P<0.05),with BE yielding the highest at 43.13±0.26%,higher than that of RE(34.29±0.81%).Ultrasound-assisted extraction significantly increased the DMY content of the extracts(P<0.05),whereas DMY content in the RE extracts was 59.94±1.70%,that of URE reached 66.14±2.78%.Analysis revealed 17 amino acids,with L-serine and aspartic acid being the most abundant in the extracts,nevertheless ultrasound-assisted extraction reduced total free amino acid *** chromatography-mass spectrometry analysis demonstrated an increase in the diversity and quantity of compounds in the vine tea water extracts obtained through ultrasonic-assisted ***,69 and 68 volatile compounds were found in URE and UBE extracts,which were higher than the number found in RE and BE *** vitro,antioxidant activity assessments revealed varying antioxidant capacities among different extraction methods,with RE exhibiting the highest DPPH scavenging rate,URE leading in ABTS•+free radical scavenging,and BE demonstrating superior ferric ion reducing antioxidant ***:The findings suggest that extraction methods signi

关键词： vine tea dihydromyricetin ultrasonic-assisted extraction volatile aroma components

miRNA-21-5p is an important contributor to the promotion of injured peripheral nerve regeneration using hypoxia-pretreated bone marrow-derived neural crest cells

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Neural Regeneration Research 2025年第1期20卷 277-290页

作者： Meng Cong Jing-Jing Hu Yan yu xiao-Li Li xiao-Ting Sun Li-Ting Wang Xia Wu Ling-Jie Zhu xiao-Jia Yang Qian-Ru He Fei Ding Hai-Yan Shi School of Medicine Nantong UniversityNantongJiangsu ProvinceChina Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education and Coinnovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina Department of Physiology Jiangsu Health Vocational CollegeNanjingJiangsu ProvinceChina Department of Pathology Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina

Our previous study found that rat bone marrow–derived neural crest cells(acting as Schwann cell progenitors)have the potential to promote long-distance nerve ***-based therapy can enhance peripheral nerve repair and regeneration through paracrine bioactive factors and intercellular ***,the complex contributions of various types of soluble cytokines and extracellular vesicle cargos to the secretome remain *** investigate the role of the secretome and extracellular vesicles in repairing damaged peripheral nerves,we collected conditioned culture medium from hypoxia-pretreated neural crest cells,and found that it significantly promoted the repair of sensory neurons damaged by oxygen-glucose *** mRNA expression of trophic factors was highly expressed in hypoxia-pretreated neural crest *** performed RNA sequencing and bioinformatics analysis and found that miR-21-5p was enriched in hypoxia-pretreated extracellular vesicles of neural crest ***,to further clarify the role of hypoxia-pretreated neural crest cell extracellular vesicles rich in miR-21-5p in axonal growth and regeneration of sensory neurons,we used a microfluidic axonal dissociation model of sensory neurons in vitro,and found that hypoxia-pretreated neural crest cell extracellular vesicles promoted axonal growth and regeneration of sensory neurons,which was greatly dependent on loaded ***,we constructed a miR-21-5p-loaded neural conduit to repair the sciatic nerve defect in rats and found that the motor and sensory functions of injured rat hind limb,as well as muscle tissue morphology of the hind limbs,were obviously *** findings suggest that hypoxia-pretreated neural crest extracellular vesicles are natural nanoparticles rich in ***-21-5p is one of the main contributors to promoting nerve regeneration by the neural crest cell *** helps to explain the mechanism of action of the secretome and e

关键词： axotomy cell-free therapy conditioned medium extracellular vesicles hypoxic preconditioning microRNA oxygen-glucose deprivation peripheral nerve injury Schwann cell precursors

Reversible Chain Transfer Catalyzed Polymerization with Alkyl Iodides Generated from Alkyl Bromides by in Situ Halogen Exchange

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Chinese Journal of Polymer Science 2021年第9期39卷 1161-1168页

作者： Dan-Ni Gao yu-Lai Zhao Jing-yu cai Lin-Xi Hou long-Qiang xiao Department of Materials-Oriented Chemical Engineering School of Chemical EngineeringFuzhou UniversityFuzhou 350116China Qingyuan Innovation Laboratory Quanzhou 362801China Fujian Key Laboratory of Advanced Manufacturing Technology of Specialty Chemicals Fuzhou UniversityFuzhou 350116China

Reversible chain transfer catalyzed polymerization(RTCP)is a practical and efficient process for the precise synthesis of polymers with special architecture by using simple phenols(2,4,6-trimethylphenol,TMP)or hydrocarbons(xanthene,XT)as efficient ***,alkyl iodide(R-1),which was gen erated from in situ bromine-iodine transformation of alkyl bromide(R-Br)with sodium iodide(Nal),was served as initiator to mediate RTCP with TMP or *** and other functional methacrylates,including GMA,DEAM,DMAEMA and BzMA,were successfully initiated by combining orga no catalysts and azo in itiators to yield polymers with low-polydispersity(M_(w)/M_(n)=1.1-1.5)and ideal mono mer conversions(50%-90%)at moderate *** over,3-armstar polymers were also obtained by this *** high chain-end fidelity of the obtained poly(methyl methacrylate)with iodine as chain-end group(PMMA-I)was confirmed by chain-extension *** en vironme ntally frie ndly initiators and orga no catalysts exhibit powerful polymerization properties toward RTCP,providing a sign ificant method to synthesize functional polymers.

关键词： Reversible chain transfer catalyzed polymerization Organocatalyst Halogen exchange

Exploration of the structural and optical properties of a red-emitting phosphor K_2TiF_6:Mn^(4+)

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Chinese Physics B 2019年第1期28卷 544-549页

作者： Xi-long dou xiao-yu Kuang Xin-Xin Xia Meng Ju Institute of Atomic and Molecular Physics Sichuan University School of Physical Science and Technology Southwest University

The exploration of the appropriate red phosphor with good luminescence properties is an important issue in the development of current white light-emitting diode(WLED) devices. Transition metal Mn-doped compounds are fascinating luminescent materials. Herein, we performed a systematic theoretical study of the microstructure and optical properties of K_2TiF_6:Mn^(4+) using the CALYPSO structure search method in combination with first-principles calculations. We uncovered a novel structure of K_2TiF_6:Mn^(4+) with space group P-3m1 symmetry, where the impurity Mn_(4+) ions are accurately located at the center of the MnF_6 octahedra. Based on our developed complete energy matrix diagonalization(CEMD)method, we calculated transition lines for ~2E_g →~4A_2,~4A_2→~4T_2, and^4A_2→T2at 642 nm, 471 nm, and 352 nm,respectively, which are in good agreement with the available experimental data. More remarkably, we also found another transition(~4A_2→~2T_2) that lies at 380 nm, which should be a promising candidate for laser action.

关键词： crystal structures first-principles calculations K2TiF6

Structural and magnetic properties of ZnFe_2O_4 films deposited by low sputtering power

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International Journal of Minerals,Metallurgy and Materials 2012年第10期19卷 964-968页

作者： Jin-long Li Zhong yu Ke Sun xiao-na Jiang Zhong-wen lan State Key Laboratory of Electronic Thin Films and Integrated Devices University of Electronic Science and Technology of China Chengdu 610054 China No. 24 Research Institute of China Electronics Technology Group Corporation Chongqing 400060 China

To validate the correctness of the Hartman-Perdok Theory （HPT）, which indicates that the { 111 } planes have the lowest surface energy in spinel ferrites, the {111} plane orientated ZnFe204 thin films on Si（100）, Si（111）, and SIO2（500 nm）/Si（111） substrates were obtained through a radio frequency （RF） magnetron sputtering method with a low sputtering power of 80 W. All of the experiments prove that the atom energy determined by sputtering power plays an important role in the orientated growth of the ZnFe204 thin films, and it matches well with HPT. The ZnFe2O4 thin films exhibit ferromagnetism with a magnetization of 84.25 ld/mol at room temperature, which is different from the bulk counterpart （antiferromagnetic as usual）. The ZnFe204 thin films can be used as high-quality oriented inducing buffer layers for other spinel （Ni, Mn）Zn ferrite thin films and may have high potential in magnetic thin films-based devices.

关键词： thin films magnetron sputtering crystal orientation film growth surface energy ferromagnetism

基于O'NET半结构化访谈建构大学生职业适应力故事

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高校辅导员学刊 2025年第1期17卷 34-42,97页

作者：林振余权肖龙福建商学院传媒与会展学院福建福州350012

职业适应力是大学生转型为职场人士的关键能力,其中职业兴趣是核心要素。然而,当前对大学生职业兴趣的分析主要局限于定量评估,导致学生对自身职业兴趣的理解较为表面,甚至开始怀疑其价值。研究采用O'NET半结构化访谈,结合主题演绎驱动... 详细信息

职业适应力是大学生转型为职场人士的关键能力,其中职业兴趣是核心要素。然而,当前对大学生职业兴趣的分析主要局限于定量评估,导致学生对自身职业兴趣的理解较为表面,甚至开始怀疑其价值。研究采用O'NET半结构化访谈,结合主题演绎驱动理论,深入分析了大学生在职业适应力故事中的关注、控制、好奇、自信、合作五个维度,旨在探讨半结构化访谈如何定性地将职业兴趣代码与这五个维度的故事关联起来,并揭示其在提升大学生职业适应力方面的作用。研究结果显示,通过半结构化访谈建构的职业适应力故事,成功整合了定量与定性职业兴趣评估,这种方法不仅能帮助大学生更深入地理解自身的霍兰德职业兴趣代码,还能促使他们系统地叙述职业适应力故事,从而有效提升其职业适应力。

关键词：故事建构半结构化访谈职业适应力定性 O'NET

Fuzzy Zorn’s lemma with applications

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Applied Mathematics(A Journal of Chinese Universities) 2021年第4期36卷 521-536页

作者： XIN xiao-long FU yu-long School of Science Xi’an Polytechnic UniversityXi’an 710048China School of Mathematics Northwest UniversityXi’an 710127China School of Cyber Engineering Xidian UniversityXi’an 710071China

We introduced the fuzzy axioms of choice,fuzzy Zorn’s lemma and fuzzy well-ordering principle,which are the fuzzy versions of the axioms of choice,Zorn’s lemma and well-ordering principle,and discussed the relations among *** an application of fuzzy Zorn’s lemma,we got the following results:(1)Every proper fuzzy ideal of a ring was contained in a maximal fuzzy ideal.(2)Every nonzero ring contained a fuzzy maximal ideal.(3)Introduced the notion of fuzzy nilpotent elements in a ring R,and proved that the intersection of all fuzzy prime ideals in a commutative ring R is the union of all fuzzy nilpotent elements in R.(4)Proposed the fuzzy version of Tychonoff Theorem and by use of fuzzy Zorn’s lemma,we proved the fuzzy Tychonoff Theorem.

关键词： fuzzy axioms of choice fuzzy Zorn’s lemma fuzzy well-ordering principle fuzzy ideal of a ring fuzzy Tychonoff Theorem

Modified laparoscopic Sugarbaker repair of parastomal hernia with a three-point anchoring technique

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World Journal of Clinical Cases 2018年第14期6卷 759-766页

作者： Di-yu Huang long Pan Qi-long Chen xiao-Yan cai Jie Fang Institute of Minimally Invasive Surgery Zhejiang University Hangzhou 310016 Zhejiang Province China Clinical Medicine Research Center for Minimally Invasive Diagnosis and Treatment of Abdominal Viscera of Zhejiang Province Hangzhou 310016 Zhejiang Province China Department of General Surgery Sir Run Run Shaw Hospital Zhejiang University Hangzhou 310016 Zhejiang Province China Department of General Medicine Sir Run Run Shaw Hospital Zhejiang University Hangzhou 310016 Zhejiang Province China

AIMTo retrospectively evaluate the safety and feasibility of a new modified laparoscopic Sugarbaker repair in patients with parastomal *** retrospective study was performed to analyze eight patients who underwent parastomal hernia repair between June 2016 and January 2018. All of these patients received modified laparoscopic Sugarbakerhernia repair treatment. This modifed technique included an innovative three-point anchoring and complete su-turing technique to fix the mesh. All procedures were performed by a skilled hernia surgeon. Demographic data and perioperative outcomes were collected to eva-luate the safety and effcacy of this modifed *** these eight patients, two had concomitant incisional hernias. All the hernias were repaired by the modifed laparoscopic Sugarbaker technique with no conversion to laparotomy. Three patients had in-situ reconstruc-tion of intestinal stoma. The median mesh size was 300 cm2, and the mean operative time was 205.6 min. The mean postoperative hospitalization time was 10.4 d, with a median pain score of 1 （visual analog scale method） at postoperative day 1. Two patientsdeveloped postoperative complications. One patient had a pocket of effusion surrounding the biologic mesh, and one patient experienced an infection around the reconstructed stoma. Both patients recovered after conservative management. There was no recurrence during the follow-up period （6-22 mo, average 13 mo）.CONCLUSIONThe modifed laparoscopic Sugarbaker repair could fx the mesh reliably with mild postoperative pain and a low recurrence rate. The technique is safe and feasible for parastomal hernias.

关键词： Parastomal hernia Three-point anchoring and suturing Sugarbaker repair Mesh Enterostomy

Expression and characterization of a CALB-type lipase from Sporisorium reillianum SRZ2 and its potential in short-chain flavor ester synthesis

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Frontiers of Chemical Science and Engineering 2020年第5期14卷 868-879页

作者： Jiang-Wei Shen Xue cai Bao-Juan dou Feng-yu Qi xiao-Jian Zhang Zhi-Qiang Liu yu-Guo Zheng The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chermicals Zhejiang University of TechnologyHangzhou 310014China Key Laboratory of Bioorganic Synthesis of Zhejiang Province College of Biotechnology and Bioengineering.Zhejiang University of TechnologyHangzhou 310014China

A lipase from Sporisorium reilianum SRZ2(SRL)with 73%amino acid sequence identity to Candida antarctica lipase B(CALB)was cloned and overexpressed in Pichia *** recombinant SRL showed a preference for short-chain p-nitrophenyl *** achieved maximum activity at pH 8.0 and 65°C for p-nitrophenyl hexanoate(C6)with Km and kcar/Km values of 0.14 mmol·L-1 and 1712 min ***·L-1 at 30℃,*** displayed excellent thermostability and pH stability,retaining more than 79%of its initial activity after incubation at 60℃ for 72h and 75%atpH 3to 11 for 72 *** also maintained most of its activity in the presence of inhibitors and detergents except sodium dodecyl sulfate,and it tolerated organic *** was covalently immobilized and successfully used for ethyl hexanoate synthesis in cyclohexane or in a solvent-free system with a high conversion yield(>95%).Furthermore,high con-version yield was also achieved for the synthesis of various short-chain flavor esters when high substrate concentra-tions of2 *** were *** study indicated that ***-type lipase from *** SRZ2 showed great potential in organic ester synthesis.

关键词： lipase Sporisorium reiliamum biochemical characterization short-chain flavor ester solvent-free system