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Journal of Ocean University of China 2023年第6期22卷 1621-1634页

作者： MEI Yaoping HOU Zhishuai GAO Qinfeng dong shuanglin LI Xueqi XU Yuling Key Laboratory of Mariculture Ministry of EducationOcean University of ChinaQingdao 266003China Function Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and TechnologyQingdao 266235China

Sea cucumber(Apostichopus japonicus)is an excellent model for investigating effects of bottom-dwellers on carbon mig-ration and ***,the molecular mechanism of respiratory metabolism process variation caused by feeding rations is poorly *** this study,treatment groups set as 1%(about 0.63g),3%,and 7%of total body weight(named F1,F3 and F7 groups respectively).The potential molecular mechanisms behind the functions of respiratory tree and body wall were investigated by RNA-Seq.A total of 52411 expressed genes were identified from 89342 expressed *** results showed 759,254 and 334 genes were up-regulated,and 334,445 and 992 genes were down-regulated in respiratory tree of F1 vs.F3,F1 vs.F7 and F3 vs.F7,***,2070,1601 and 896 genes were up-regulated,and 1303,1337 and 1144 genes were down-regulated in body wall between F1 vs.F3,F1 vs.F7 and F3 vs.F7,*** expressed genes were enriched in salivary secretion and ECM-receptor interaction pathways in respiratory tree,and in various types of N-glycan biosynthesis,ribosome and sphingolipid metabolism pathways in body *** results suggested respiratory tree and body wall were involved in activation of respiratory metabolisms in response to different feeding *** research provided valuable knowledge for physiological differences in res-piratory metabolism.

关键词： transcriptomics Apostichopus japonicus feeding ration respiration metabolism

Effect of Temperature on the Carbon, Nitrogen, and Phosphorus Nutrient Budgets of Steelhead Trout (Oncorhynchus mykiss) with Different Sizes

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Journal of Ocean University of China 2022年第5期21卷 1283-1294页

作者： ZHANG Gong GAO Qinfeng ZHOU Yangen dong shuanglin WANG Meijun GAO Yonggang Key Laboratory of Mariculture Ministry of EducationOcean University of ChinaQingdao 266003China Function Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and TechnologyQingdao 266100China Fishery Technology Promotion Station of Rongcheng City Weihai 264300China

This study was performed to evaluate the effect of temperature on the carbon(C),nitrogen(N),and phosphorus(P)nu-trient budgets of adult steelhead trout(Oncorhynchus mykiss)with different body sizes.A complete two-factor experiment was con-ducted at four temperatures,i.e.,9(T9),13(T13),16(T1℃℃℃6),and 19(T19);and three body sizes,℃i.e.,0.65 kg±0.02 kg(S1),1.22 kg±0.06 kg(S2),and 2.05 kg±0.09 kg(S3).The following properties of steelhead trout were determined:C,N,and P nutrient budgets;feed intake rate;fecal production rate;oxygen consumption rate;ammonia excretion rate;phosphorus excretion rate;scope of the growth of C,N,and P;and absorption efficiency of C,N,and *** showed no significant interaction between temperature and body size in terms of the nutrient budgets of steelhead ***,temperature and body size played important separate roles in the nutrient budgets except for the absorption efficiency of *** feed intake rate,fecal production rate,ammonia excretion rate,phosphorus excretion rate,and scope of the growth of C,N,and P were the highest in the T16 *** absorption effi-ciency of C and P decreased as temperature *** multiple regression results indicated that 15 was the approximate℃optimal temperature for the feeding and growth of steelhead *** feed intake rate,fecal production rate,oxygen consumption rate,ammonia excretion rate,phosphorus excretion rate,and scope of the growth of C,N,and P per unit of the body weight of fish decreased as body size *** absorption efficiency of C increased as body weight increased,and the absorption efficiencies of P in the S2 and S3 treatments were significantly higher than those in the S1 *** the culture of steelhead trout,the daily ratio should be adjusted with changes in the temperature and body size of ***,managers should focus on the risk of hypoxia at high water temperatures.

关键词： steelhead trout temperature body size nutrient budget

3种不同添加物对凡纳滨对虾生长、非特异免疫和抗病力的影响

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中国海洋大学学报（自然科学版） 2024年第2期54卷 33-43页

作者：田相利秦光彩罗凯汪仕爽刘杨魏聪王明阳董双林刘清兵海水养殖教育部重点实验室(中国海洋大学) 山东青岛266003 崂山实验室山东青岛266237 青岛瑞滋集团有限公司山东青岛266408

为评估3种不同添加物对凡纳滨对虾(Litopenaeus vannamei)生长性能、非特异免疫反应及抗病力的影响,在基础饲料中分别添加了丁酸梭菌CBG01(Clostridium butyricum CBG01)活菌(CB组)、3%聚β-羟基丁酸酯(PHB组)和1%丁酸钠(BS组)来投喂对... 详细信息

为评估3种不同添加物对凡纳滨对虾(Litopenaeus vannamei)生长性能、非特异免疫反应及抗病力的影响,在基础饲料中分别添加了丁酸梭菌CBG01(Clostridium butyricum CBG01)活菌(CB组)、3%聚β-羟基丁酸酯(PHB组)和1%丁酸钠(BS组)来投喂对虾,对照组投喂基础饲料,养殖6周后测量对虾生长情况、检测血清非特异免疫指标和肝胰腺免疫相关基因表达水平,进行副溶血弧菌(Vibrio parahaemolyticus)攻毒实验。研究表明,CB组和PHB组对虾末体质量和特定生长率显著高于对照组(P0.05)。3个处理组对虾成活率和饲料效率均显著高于对照组(P0.05)。副溶血弧菌攻毒实验表明,3个处理组对虾的累积死亡率均显著低于对照组(P<0.05)。研究结果表明,饲料中添加丁酸梭菌、PHB和丁酸钠均可不同程度地提高凡纳滨对虾的生长性能和非特异免疫能力,能够显著提高对虾对副溶血弧菌感染的抵抗力。总体比较,添加丁酸梭菌和PHB的作用效果基本相当,在一定程度上要优于添加丁酸钠。在饲料生产加工过程中,在难以添加丁酸梭菌活菌的情况下,建议适当添加聚β-羟基丁酸酯来替代丁酸梭菌。

关键词：丁酸梭菌聚β-羟基丁酸酯丁酸钠凡纳滨对虾生长非特异性免疫抗病力副溶血弧菌

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Effects of Starving and Re-feeding Strategies on the Growth Performance and Physiological Characteristics of the Juvenile Tongue Sole(Cynoglossus semilaevis)

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Journal of Ocean University of China 2017年第3期16卷 517-524页

作者： FANG Ziheng TIAN Xiangli dong shuanglin Key Laboratory of Mariculture of Ministry of Education College of Fisheries Ocean University of ChinaQingdao 266003 P. R. China

Starving and re-feeding trials were conducted to evaluate the effects of starvation duration and recovery times on the growth performance and physiological characteristics of juvenile tongue sole Cynoglossus semilaevis, which included growth, body composition, intestinal morphology and digestive enzymes activities. The fish starved for one(D1), two(D2) and four(D4) days, respectively, were recovered for three(R3, R6, R12), seven(R7, R14, R28) and eleven(R11, R22, R44) folds of starvation days while the control fish were fed constantly. The experiment was conducted for 96 days. The fish individuals in D1R11, D2R14 and D2R22 caught up in weight with the control, indicating that complete compensatory growth existed in these fish individuals. The highest body weight gain and total specific growth rates were observed in D2R22. Food intake increased significantly in most treatments in comparison with that of the control except for D1R7 and D2R22(P < 0.05). Food conversion efficiency and apparent digest coefficient in D2R22 were significantly promoted, being higher than that of the control(P < 0.05). The activities of trypsin and lipase were found to be closely related to the growth performance. The activities of liver and hindgut trypsin, also midgut and hindgut lipase in D2R22 were significantly higher than those of the control(P < 0.05). The fold height(HF) of foregut and midgut increased significantly in D2R22, and HF decreased significantly in D1R3, D2R6 and D4R12(P < 0.05). On our findings, we may conclude that the optimum starving and re-feeding strategy is starving for 2 days and re-feeding for 22 days. And in this starving and re-feeding strategy, the compensatory growth could be mostly attributed to the promotion of food conversion efficiency and digestibility coefficiency.

关键词：饥饿重新喂生长性能消化的酶肠的形态学 Cynoglossus semilaevis

Effect of Food on Specific Dynamic Action(SDA) of Green and Red Types of Sea Cucumber(Apostichopus japonicus Selenka)

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Journal of Ocean University of China 2017年第5期16卷 905-910页

作者： BAO Jie JIANG Hongbo dong shuanglin TIAN Xiangli Key Laboratory of Mariculture of Ministry of Education Ocean University of China College of Animal Science and Veterinary Medicine Shenyang Agricultural University

Specific dynamic action(SDA), the energy expended on all physiological processes that is associated with meal digestion and absorption, is strongly affected by food type. Effects of formulated diet(FMD), macroalgae(ALG) and sea mud(SMD) diets on the postprandial metabolic response of the green type and the red type of sea cucumber(Apostichopus japonicus) were studied in order to understand their feeding physiology. Food offered to A. japonicus was different in protein, lipid content and energy but not in meal mass. SDA of A. japonicus resulted in a 1.3–2.7 folds of increase in oxygen consumption that can persist for up to 4.8–31.7 h after digesting three different diets. In a given type of sea cucumber, the magnitude of SDA was the highest when fed with FMD, medium with ALG, and the lowest with SMD, which is probably due to the differences in diet components and protein contents. The red type sea cucumber showed greater SDA magnitude than the green type with each diet treatment, which might result from the difference in factorial scope between the two types of sea cucumber. However, the smallest magnitude or even no difference was observed between the two types of A. japonicus in SMD group, perhaps owing to the poor nutrition and digestion of sea mud.

关键词：特殊动力作用食物消化食物类型 SDA 海参氧气消耗量蛋白质含量生理过程

Effect of Bacillus baekryungensis YD13 Supplemented in Diets on Growth Performance and Immune Response of Sea Cucumber(Apostichopus japonicus)

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Journal of Ocean University of China 2014年第5期13卷 805-810页

作者： YAN Fajun TIAN Xiangli dong shuanglin The Key Laboratory of Mariculture of Ministry of Education Fisheries College Ocean University of China

The effect of a potential probiotic on the growth performance and immune response of sea cucumber(Apostichopus japonicus) was investigated. Bacillus baekryungensis YD13 isolated from sea cucumber culturing ponds was added to sea cucumber basal feed as a probiotic in different doses(0, the control; 1×104(YD134), 1×106(YD136) and 1×108(YD138) CFU g-1 of diet), and administered orally to A. japonicus(initial mean wet weight 5.44 g ± 0.17 g). The sea cucumbers were fed in 20 aquaria, 5 each treatment, for 60 d. At the end of growth trial, 20 sea cucumbers from each treatment were challenged with Vibrio splendidus. A. japonicus in YD134 and YD136 exhibited significantly better growth performance than control(P < 0.05). Five non-specific immune parameters including lysozyme, acid phosphatase, alkaline phosphatase, superoxide dismutase and catalase in coelomic fluid were measured to evaluate the immune response of A. japonicus to the probiotics. Results showed that all parameters were significantly improved when YD11 was supplemented in the dose of 1×106 CFU g-1(P < 0.05). The cumulative incidence and mortality after the Vibrio splendidus challenge decreased significantly in sea cucumbers of YD136. Accordingly, 1×106 CFU g-1 of YD13 in diet was recommended for the growth promotion and immune enhancement of A. japonicus.

关键词：免疫应答生长性能芽孢杆菌海参刺参超氧化物歧化酶日粮加味

An Effective Method of Prompting Juvenile Rainbow Trout (Oncorhynchus mykiss) to Cope with Heat Stress

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Journal of Ocean University of China 2020年第1期19卷 216-224页

作者： JIANG Xuyang dong shuanglin ZHOU Yangen GAO Qinfeng PAN Zhe Key Laboratory of Mariculture of Ministry of Education Ocean University of ChinaQingdao 266100China Function Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and TechnologyQingdao 266235China

In the present study,juvenile rainbow trout(Oncorhynchus mykiss)were acclimated at 5 *** in the control group(C0)were reared at a constant temperature(16℃);trout in four other treatments(A0,A3,A6,and A9)were acclimated to a high temperature(22℃)for 0,3,6,and 9 d,respectively,and then returned to normal temperature(16℃)for *** temperature was then raised to 20℃and the fish were cultured for 40 *** results showed that the growth rates of A3,A6,and A9 were higher than that of A0 but lower than that of *** growth rate of A9 was the highest among the 4 acclimation *** activities of serum transaminase and liver antioxidant enzyme significantly increased during *** the beginning of growth,the activities of enzymes were lower in A3,A6,and A9 with the lowest in *** addition,the transcript abundance of heat shock protein(HSP)60 gene in A9 was not significantly different from that of C0 during ***70 in A9 significantly increased at the beginning and returned to that of C0 at the *** findings indicated that pre-acclimation improved the high temperature tolerance with the best effectiveness observed at 22℃for 9 d.A possible mechanism underlining such phenomenon is the improvement of antioxidant defense system.

关键词： temperature acclimation enzyme activity growth performance heat shock protein rainbow trout

DNA Barcoding and Mini-DNA Barcoding Reveal Mislabeling of Salmonids in Different Distribution Channels in the Qingdao Area

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Journal of Ocean University of China 2021年第6期20卷 1537-1544页

作者： HAN Cui dong shuanglin LI Li GAO Qinfeng ZHOU Yangen Key Laboratory of Mariculture Ministry of EducationOcean University of ChinaQingdao 266003China Function Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and TechnologyQingdao 266235China

There is an increasing demand for salmonid authentication due to the globalization of the salmonid *** barcoding and mini-DNA barcoding are widely used for identifying fish species based on a fragment of the mitochondrial cytochrome c oxidase subunit I(COI)*** this study,rainbow trout(Oncorhynchus mykiss),steelhead trout(***),and Atlantic salmon(Salmo salar)collected from two salmonid aquaculture bases in China were authenticated by DNA barcoding(about 650 bp)and mini-DNA barcoding(127 bp)to evaluate the accuracy of the two methods in the identification of different salmonid *** results revealed that both methods could effectively distinguish *** and *** with 100%***,the two methods failed to separate rainbow trout(***)and steelhead trout(***),which are the same species but cultured in different water ***,salmonid samples from three main distribution channels in the Qingdao area(traditional supermarkets,online supermarkets,and sushi bars)were identified by the two *** of *** with *** was discovered,and the 27.78%overall substitution rate of salmonids in the Qingdao area was higher than those in other regions reported in previous *** addition,the mislabeling rates of salmonids from traditional supermarkets,online supermarkets,and sushi bars were compared in this *** mislabeling rate was significantly greater in sushi bars(50%)than in the other two channels(16.67%),suggesting that stronger monitoring and enforcement measures are necessary for the aquatic food catering industry.

关键词： salmonid DNA barcoding mini-DNA barcoding species authentication mislabeling rate

Growth, Metabolism and Physiological Response of the Sea Cucumber, Apostichopus japonicus Selenka During Periods of Inactivity

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中国海洋大学学报：英文版 2013年第1期12卷 146-154页

作者： DU Rongbin ZANG Yuanqi TIAN Xiangli dong shuanglin School of Oceanography Yantai University The Key Laboratory of Mariculture Ministry of Education of China Ocean University of China Provincial Marine Fisheries Research Institute of Shandong

The growth, metabolism and physiological response of the sea cucumber, Apostichopus japonicus, were investigated during periods of inactivity. The body weight, oxygen consumption rate (OCR), activities of acidic phosphatase (ACP), alkaline phosphatase (AKP), catalase (CAT) and superoxide dismutase (SOD), and content of heat shock protein 70 (Hsp70) in the body wall and coelomic fluid of A. japonicus were measured during starvation, experimental aestivation and aestivation. The results showed that the body weight of sea cucumber in the three treatments decreased significantly during the experimental period (P < 0.05). The OCR of sea cucumber reduced in starvation and experimental aestivation treatments, but increased gradually in natural aestivation treatment. The activities of ACP and AKP of sea cucumber decreased gradually in all treatments, whereas those of SOD and CAT as well as Hsp70 content decreased in the starvation and experimental aestivation treatments and increased in natural aestivation treatment. The sea cucumber entered a state of aestivation at 24℃. To some extent, the animals in experimental aestivation were different from those in natural aestivation in metabolism and physiological response. These findings suggested that the aestivation mechanism of A. japonicus is complex and may not be attributed to the elevated temperature only.

关键词：生理反应闲置期间海参代谢生长热休克蛋白70 超氧化物歧化酶酸性磷酸酶

Virus-phytoplankton adhesion:a new WSSV transmission route to zooplankton

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Acta Oceanologica Sinica 2007年第6期26卷 109-115页

作者： ZHANG Jiasong dong shuanglin TIAN Xiangli dong Yunwei LIU Xiangyi YAN dongchun Maricuhure Research Laboratory Fisheries College Ocean University of China Qingdao 266003 China Key laboratory for Sustainable Utilization of Marine Fisheries Resources of Ministry of Agriculture Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Qingdao 266071 China Science and Technology Bureau of Rizhao Rizhao 276800 China Department of Life Science Ludong University Yantai 264025 China

The pathogenicity of white spot syndrome virus （WSSV） to zooplankton species, rotifer Brachionus urceus （Linnaeus）, copepod Acartia clausi （Giesbrecht） and mysid shrimp Neomysis awatschensis （ Brandt ）, was estimated by immersion challenge and virus - phytoplankton adhesion mute to investigate a potential new transmission mute of WSSV to zooplankton. WSSV succeeded in infecting these zooplankton species and nested-PCR revealed positive results for the virus - phytoplankton adhesion mute, whereas WSSV cannot infect zooplankton by immersion challenge. These results indicated that virus - phytoplankton adhesion route is a successful new transmission mute of WSSV to zooplankton and also implied that phytoplankton could be a carrier in WSSV transmission.

关键词： virus - phytoplankton adhesion WSSV transmission route zooplankton