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脂肪量和肥胖相关基因对骨关节炎软骨细胞凋亡和炎症反应的影响

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中华老年医学杂志 2024年第2期43卷 221-227页

作者：董莉妮何浩雨邝磊陈泽军王肖枭王冰吕国华中南大学湘雅二医院老年科长沙410011 中南大学湘雅二医院脊柱外科长沙410011

目的探究脂肪量和肥胖相关基因(FTO)对骨关节炎(OA)软骨细胞凋亡和炎症反应的影响。方法分析人正常软骨组织样本与OA软骨组织样本中FTO的表达差异,分离并培养原代OA软骨细胞,并构建OA大鼠模型,从临床、动物和细胞水平检测FTO表达。利用... 详细信息

目的探究脂肪量和肥胖相关基因(FTO)对骨关节炎(OA)软骨细胞凋亡和炎症反应的影响。方法分析人正常软骨组织样本与OA软骨组织样本中FTO的表达差异,分离并培养原代OA软骨细胞,并构建OA大鼠模型,从临床、动物和细胞水平检测FTO表达。利用FTO敲低慢病毒(sh-FTO)和m^(6)A甲基化抑制剂(cycloleucine)处理细胞,检测细胞中m^(6)A含量和炎症因子白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)的表达水平;流式细胞术检测OA软骨细胞凋亡情况,蛋白质免疫印迹(Western blot)检测凋亡蛋白B细胞淋巴瘤-2(Bcl-2)、Bcl-2相关X蛋白(Bax)的表达水平。结果与正常对照比较,人OA软骨组织、OA大鼠软骨组织及OA软骨细胞中FTO的mRNA和蛋白表达均显著上升(均P<0.05)。敲低FTO后,原代OA软骨细胞中m^(6)A水平上升,IL-6、TNF-α水平显著降低,细胞凋亡率降低,凋亡蛋白Bax表达显著下调,Bcl-2表达显著上调;而cycloleucine干预能显著降低OA软骨细胞中m^(6)A水平,增加IL-6、TNF-α的丰度,促进细胞凋亡和凋亡相关蛋白表达,逆转FTO敲低慢病毒产生的干预效果(均P<0.05)。结论FTO可能介导m^(6)A机制促进OA软骨细胞凋亡和炎症反应,进而加速OA的进展。

关键词：骨关节炎软骨细胞炎症细胞凋亡

PPARγ激动剂对糖尿病大鼠载脂蛋白M分泌和表达的影响

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中南大学学报（医学版） 2012年第8期37卷 796-801页

作者：屈晓冰赵水平高洁胡敏董莉妮张湘瑜中南大学湘雅二医院老年病科长沙410011 中南大学湘雅二医院心内科长沙410011 第二军医大学附属长海医院上海200433 中南大学湘雅二医院检验科长沙410011

目的:研究高选择性过氧化物酶增殖体激活型受体γ(PPARγ)激动剂罗格列酮(rosiglitazone,RSG)对糖尿病大鼠血清载脂蛋白M(apoM)水平和肝、肾、脂肪组织apoM mRNA表达的影响。方法:雄性SD大鼠分为对照组(Con组,n=7)、高脂组(HF组,n=8)、... 详细信息

目的:研究高选择性过氧化物酶增殖体激活型受体γ(PPARγ)激动剂罗格列酮(rosiglitazone,RSG)对糖尿病大鼠血清载脂蛋白M(apoM)水平和肝、肾、脂肪组织apoM mRNA表达的影响。方法:雄性SD大鼠分为对照组(Con组,n=7)、高脂组(HF组,n=8)、糖尿病组(DM组,n=7)和糖尿病RSG干预组(RSG组,n=7)4组。实验模型建立前采血检测各组大鼠空腹血糖(FBG)、空腹胰岛素(FINS)、三酰甘油(TG)、总胆固醇(TC)。糖尿病大鼠模型的建立参照周氏等方法,给予高脂高糖饲料喂养、腹腔注射链脲佐菌素。应用RSG对糖尿病大鼠干预治疗8周。全部动物于实验第15周结束时集中处死,搜集血标本和肝、肾、脂肪组织标本。检测各组大鼠的血FBG,FINS,TG,TC浓度。ELISA检测各组大鼠的血apoM水平,并与血FBG,FINS,TG,TC水平进行相关分析。RT-PCR测定各组大鼠肝、肾、脂肪组织apoM mRNA的表达。结果:与Con组和HF组比较,DM组大鼠血清apoM水平明显降低(P<0.05),RSG组大鼠的血清apoM水平较DM组明显升高(P<0.05)。各组大鼠以肝组织apoM mRNA的表达最高,肾组织次之,脂肪组织的表达最低。与Con组比较,HF组、DM组和RSG组的大鼠肝、肾、脂肪组织apoM mRNA的表达明显减少(P<0.05),RSG组大鼠肝、肾、脂肪组织apoM mRNA的表达较DM组均有明显增加(P<0.05)。大鼠血清apoM水平与血清TG(r=–0.466,P=0.011),TC(r=–0.568,P=0.001),FBG(r=–0.371,P<0.001),FINS(r=–0.768,P=0.048)水平均呈显著负相关。结论:ApoM在血糖和血脂的代谢中起着重要作用并受PPARγ激动剂调节的影响。

关键词：载脂蛋白M PPARγ 糖尿病 SD大鼠

心房颤动老年患者血清半乳糖凝集素3的表达及对其预后的影响

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中华老年医学杂志 2022年第11期41卷 1284-1289页

作者：彭洁婷谭胜玉董莉妮张湘瑜中南大学湘雅二医院老年医学科长沙410011

目的观察心房颤动(房颤)老年患者与非房颤患者血清半乳糖凝集素3的表达水平差异,并探讨其对房颤患者预后的影响。方法回顾性研究,连续入选2018年3—9月中南大学湘雅二医院老年心血管内科年龄≥60岁患者100例,根据心电图及既往房颤病史... 详细信息

目的观察心房颤动(房颤)老年患者与非房颤患者血清半乳糖凝集素3的表达水平差异,并探讨其对房颤患者预后的影响。方法回顾性研究,连续入选2018年3—9月中南大学湘雅二医院老年心血管内科年龄≥60岁患者100例,根据心电图及既往房颤病史分为房颤组73例,非房颤组27例。采用酶联免疫吸附试剂盒检测两组患者半乳糖凝集素3的表达水平,超声心动图测量患者各房室大小指标。采用曼-惠特尼检验比较两组间半乳糖凝集素3水平,二元Logistic回归分析确定老年房颤的危险因素。采用Kaplan-Meier生存曲线、Cox比例风险回归分析生存率及半乳糖凝集素3与预后的关系。结果血清半乳糖凝集素3水平房颤组患者高于非房颤组[(395.13±113.24)ng/L比(328.53±89.11)ng/L,t=2.626,P0.05)。结论房颤老年患者半乳糖凝集素3的表达水平高于非房颤患者,半乳糖凝集素3水平与年龄、房颤时间、C反应蛋白相关。血清半乳糖凝集素3是老年人发生房颤患者的危险因素。

关键词：半乳糖凝集素3 心房颤动 C反应蛋白

他汀类药物对老年良性前列腺增生合并代谢综合征患者的疗效分析

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中华老年医学杂志 2014年第4期33卷 380-384页

作者：曾小芳屈晓冰董莉妮赵晓昆张湘瑜中南大学湘雅二医院老年病科长沙410011 北京清华大学第一附属医院心内科中南大学湘雅二医院泌尿外科长沙410011

目的探讨辛伐他汀和阿托伐他汀对老年良性前列腺增生（BPH）合并代谢综合征（MS）患者BPH临床进展的治疗作用。方法应用随机对照方法，将年龄≥60岁BPH合并MS患者135例随机分为对照组（45例）、辛伐他汀组（45例，40mg／d）和阿托伐他... 详细信息

目的探讨辛伐他汀和阿托伐他汀对老年良性前列腺增生（BPH）合并代谢综合征（MS）患者BPH临床进展的治疗作用。方法应用随机对照方法，将年龄≥60岁BPH合并MS患者135例随机分为对照组（45例）、辛伐他汀组（45例，40mg／d）和阿托伐他汀组（45例，20mg／d）。干预前和干预12个月后分别检测体质指数（BMI）、腰围、血压、空腹血糖（FBS）、糖化血红蛋白（HbAlc）、三酰甘油（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-c）、高密度脂蛋白胆固醇（HDL—C）、高敏c反应蛋白（hs—CRP）、白介素-6（IL-6）、睾酮、雌二醇、前列腺特异性抗原（PSA）水平、国际前列腺症状评分（IPSS）及前列腺体积。结果（1）与对照组比较，12个月后辛伐他汀组和阿托伐他汀组TG、TC、LDL-C水平明显下降（P〈0．05），HDLC水平明显升高（P〈0．05），hs—CRP、IL-6水平明显下降（P〈0．05），IPSS评分及前列腺体积也明显下降（P〈0．05）。前列腺体积缩小程度辛伐他汀组为（10．86±5．65）ml，阿托伐他汀组为（5．91±3．03）ml，差异有统计学意义（P-0．049）。（2）多元逐步回归分析发现，他汀类干预前后TC、IL-6水平的下降及HDL—C水平的升高与前列腺体积缩小明显相关（t=2．437，2．463，-2．952，均P〈0．05），即Tc、IL-6水平的下降程度越大及HDL-C水平的升高程度越大，则前列腺体积缩小越明显。结论辛伐他汀和阿托伐他汀治疗对BPH合并MS老年人有缩小前列腺体积、改善下尿路梗阻症状的作用，其中辛伐他汀缩小前列腺体积的作用较阿托伐他汀明显。他汀药物可能通过调脂、抗炎等机制起到延缓前列腺增生临床进展的作用。

关键词：前列腺增生代谢综合征X 羟甲基戊二酰基COA还原酶抑制剂

Introducing electrolytic electrochemical polymerization for constructing protective layers on Ni-rich cathodes of Li-ion batteries

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Rare Metals 2024年第6期43卷 2536-2545页

作者： Lin-Tao Dou Bei Li Hao-Long Nie dong-dong Xiao Chao-Qun Shang Xiao-Min Wang Zhan-Hui Zhang Katerina E.Aifantis Pu Hu School of Materials Science and Engineering Wuhan Institute of TechnologyWuhan 430205China Huairou Division Institute of PhysicsChinese Academy of SciencesBeijing 101400China Department of Mechanical and Aerospace Engineering University of FloridaGainesvilleFL 32603USA

lini_(0.8)Co_(0.1)Mn_(0.1)O_(2)(NCM811)is the most promising cathode for high-energy Li-ion batteries,despite its poor cycling stability that originates from the reactions that occur with the ***,to solve this interfacial issue,a facile electrolytic electrochemical polymerization process was introduced in this paper,and a uniform conductive electrolyte interface(polyaniline)was successfully constructed on the surface of the NCM811 porous electrode(PANI-NCM),which facilitated the charge transfer during charge/*** side reactions at the interface between the cathode and the electrolyte are suppressed,and thereby,the cycling performance and rate capability are considerably ***-NCM delivers an initial capacity of 157.2 mAh·g^(-1)as well as excellent cyclability(capacity retention of 88%after 500 cycles at 2C),whereas the capacity of the bare NCM811 has dropped to 31.3 mAh·g^(-1).In addition,polypyrrole and polythiophene also can be formed through electrolytic electrochemical polymerization process,which provides a practicable tactic to modify the interfacial stability of cathodes for high-energy Li-ion batteries.

关键词： Lithium-ion battery Ni-rich cathode In-situ polymerization Conductive polymer Electrochemical performance

双源CT评估老年急性ST段抬高型心肌梗死药物洗脱支架治疗后支架内再狭窄的价值

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分子影像学杂志 2021年第4期44卷 648-652页

作者：李灵王鑫王琼董莉妮中南大学湘雅二医院老年医学科湖南长沙410011 中南大学湘雅二医院麻醉科湖南长沙410011

目的探讨双源CT(DSCT)评估老年急性ST段抬高型心肌梗死(STEMI)药物洗脱支架治疗后支架内再狭窄的价值。方法选取2017年5月~2020年5月在我院治疗的老年STEMI药物洗脱支架治疗术后复诊患者210例,分析DSCT诊断支架内再狭窄的价值,比较支架... 详细信息

目的探讨双源CT(DSCT)评估老年急性ST段抬高型心肌梗死(STEMI)药物洗脱支架治疗后支架内再狭窄的价值。方法选取2017年5月~2020年5月在我院治疗的老年STEMI药物洗脱支架治疗术后复诊患者210例,分析DSCT诊断支架内再狭窄的价值,比较支架内再狭窄和通畅患者临床资料差异,采用Logistic回归分析影响因素。结果经冠状动脉血管造影检查确诊发生支架内再狭窄患者32例,支架内再狭窄发生率为15.24%;DSCT诊断支架内狭窄的敏感度、特异性、准确性、阳性预测值和阴性预测值分别为91.07%、95.00%、94.20%、82.26%和97.66%;DSCT判断直径≥3.0 mm支架内情况准确率高于直径<3.0 mm直径(P<0.05);支架内再狭窄患者年龄、糖尿病比例、吸烟史比例、LDL-C、支架数量和Gensini评分高于支架内通畅患者(P<0.05);Logistic回归分析结果显示:年龄、糖尿病、吸烟史和Gensini评分是老年STEMI药物洗脱支架治疗后支架内再狭窄的危险因素(OR=1.409、1.754、1.842和2.512,P<0.05)。结论DSCT评估老年STEMI药物洗脱支架治疗后支架内再狭窄有较高的应用价值,支架内再狭窄受患者年龄、糖尿病及吸烟史、Gensini评分的影响。

关键词：双源CT 老年急性ST段抬高型心肌梗死药物洗脱支架再狭窄应用价值影响因素

A weakly-solvated ether-based electrolyte for fast-charging graphite anode

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Chinese Chemical Letters 2024年第8期35卷 526-532页

作者： Xiao Zhu Yanbing Mo Jiawei Chen Gaopan Liu Yonggang Wang Xiaoli dong Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials Institute of New EnergyiChEM(Collaborative Innovation Center of Chemistry for Energy Materials)Fudan UniversityShanghai 200433China

Weakly-solvated electrolytes(WSEs)utilizing solvents with weak coordination ability offer advantages for low-potential graphite anode owing to their facile desolvation process and anions-derived inorganic-rich solid electrolyte interphase(SEI)***,these electrolytes face challenges in achieving a balance between the weak solvation affinity and high ionic conductivity,as well as between rigid inorganic-rich SEI and flexible SEI for long-term ***,we introduce 1,3-dioxolane(DOL)and lithium bis(trifluoromethanesulfonyl)-imide(LiTFSI)as functional additives into a WSE based on nonpolar cyclic ether(1,4-dioxane).The well-formulated WSE not only preserves the weakly solvated features and anion-dominated solvation sheath,but also utilizes DOL to contribute organic species for stabilizing the SEI *** from these merits,the optimized electrolyte enables graphite anode with excellent fast-charging performance(210 mAh/g at 5 C)and outstanding cycling stability(600 cycles with a capacity retention of 82.0%at room temperature and 400 cycles with a capacity retention of 80.4%at high temper-ature).Furthermore,the fabricated lini_(0.8)Co_(0.1)Mn_(0.1)O_(2)||graphite full cells demonstrate stable operation for 140 cycles with high capacity retention of 80.3%.This work highlights the potential of tailoring solvation sheath and interphase properties in WSEs for advanced electrolyte design in graphite-based lithium-ion batteries.

关键词： Weakly-solvated solvent Bisalt ether-based electrolyte Graphite anode NCM||graphite battery Interfacial optimization

Tuning Li/Ni mixing by reactive coating to boost the stability of cobalt-free Ni-rich cathode

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Journal of Energy Chemistry 2024年第10期97卷 20-29页

作者： Fanghui Du Xitong Zhang Yingchao Wang Lei Ding Pengfang Zhang Lingyang Liu dong Wang Jianzong Man Yuling Chen Yunwu Li Shandong Provincial Key Laboratory of Chemical Energy Storage and Novel Cell Technology and School of Chemistry and Chemical Engineering Liaocheng University School of Materials Science and Engineering Yancheng Institute of Technology

Cobalt-free cathode materials are attractive for their high capacity and low cost,yet they still encounter issues with structural and surface ***4,in particular,has garnered attention as an effective stabilizer for bulk and ***,the impact of interfacial reactions and elemental interdiffusion between AlPO4and lini0.95Mn0.05O2upon sintering on the bulk and surface remains *** this study,we demonstrate that during the heat treatment process,AlPO4decomposes,resulting in Al doping into the bulk of the cathode through elemental ***,PO43-reacts with the surface Li of material to form a Li3PO4coating,inducing lithium deficiency,thereby increasing Li/Ni *** suitable Li/Ni mixing,previously overlooked in AlPO4modification,plays a pivotal role in stabilizing the bulk and surface,exceeding the synergy of Al doping and *** presence of Ni2+ions in the lithium layers contributes to the stabilization of the delithiated structure via a structural pillar ***,suitable Li/Ni mixing can stabilize the lattice oxygen and electrode-electrolyte interface by increasing oxygen removal energy and reducing the overlap between the Ni3+/4+egand O2-2p *** findings offer new perspectives for the design of stable cobalt-free cathode materials.

关键词： Al doping Cobalt-free Ni-rich cathode Li/Ni mixing Li3PO4 coating Lithium-ion batteries

Stabilizing High-Nickel Cathodes via Interfacial Hydrogen Bonding Effects Using a Hydrofluoric Acid-Scavenging Separator

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Engineering 2024年第8期39卷 117-126页

作者： Shijie Zhong Liwei dong Botao Yuan Yueyao dong Qun Li Yuanpeng Ji Yuanpeng Liu Jiecai Han Weidong He National Key Laboratory of Science and Technology on Advanced Composites in Special Environments &Center for Composite Materials and StructuresHarbin Institute of TechnologyHarbin 150080China MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage School of Chemistry and Chemical EngineeringHarbin Institute of TechnologyHarbin 150001China Chongqing Research Institute Harbin Institute of TechnologyChongqing 401151China School of Mechanical Engineering Chengdu UniversityChengdu 610106China

Nickel-rich layered Li transition metal oxides are the most promising cathode materials for high-energydensity Li-ion ***,they exhibit rapid capacity degradation induced by transition metal dissolution and structural reconstruction,which are associated with hydrofluoric acid(HF)generation from lithium hexafluorophosphate *** potential for thermal runaway during the working process poses another *** are promising components to alleviate the aforementioned ***,an ultrathin double-layered separator with a 10 lm polyimide(PI)basement and a 2 lm polyvinylidene difluoride(PVDF)coating layer is designed and fabricated by combining a nonsolvent induced phase inversion process and coating *** PI skeleton provides good stability against potential thermal shrinkage,and the strong PI-PVDF bonding endows the composite separator with robust structural integrity;these characteristics jointly contribute to the extraordinary mechanical tolerance of the separator at elevated ***,unique HF-scavenging effects are achieved with the formation of-CO…H-F hydrogen bonds for the abundant HF coordination sites provided by the imide ring;hence,the layered Ni-rich cathodes are protected from HF attack,which ultimately reduces transition metal dissolution and facilitates long-term cyclability of the Ni-rich ***||NCM811 batteries(where“NCM”indicates lini_(x)Co_(y)Mn_(1-x-y)O_(2))with the proposed composite separator exhibit a 90.6%capacity retention after 400 cycles at room temperature and remain sustainable at 60℃with a 91.4%capacity retention after 200 *** adopting a new perspective on separators,this study presents a feasible and promising strategy for suppressing capacity degradation and enabling the safe operation of Ni-rich cathode materials.

关键词： Nickel-rich cathodes Composite separator HF scavenging Transition metal dissolution Long-term cyclability