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基因编辑技术在棉花种质创新和遗传改良中的应用

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生物技术通报 2024年第7期40卷 68-77页

作者：侯文婷孙琳张艳军董合忠山东师范大学生命科学学院济南250000 山东省农业科学院经济作物研究所济南250000

棉花是全球重要的经济作物和纺织工业原料,选育优良新品种是提高棉花产量、品质和植棉效益的主要途径。然而,传统育种方法在改良作物遗传特性方面存在很大的局限性,而基因编辑技术为加快棉花种质创新和遗传改良提供了契机。基因编辑技... 详细信息

棉花是全球重要的经济作物和纺织工业原料,选育优良新品种是提高棉花产量、品质和植棉效益的主要途径。然而,传统育种方法在改良作物遗传特性方面存在很大的局限性,而基因编辑技术为加快棉花种质创新和遗传改良提供了契机。基因编辑技术是一种利用人工设计的核苷酸酶,对生物体特定DNA序列的基因组片段进行删除、修改、插入或替换单个或多个核苷酸,从而实现对目标基因精确编辑的技术方法。本文首先综述了ZFNs、TALENs和CRISPR三种主要基因编辑系统的原理,以更好地理解如何利用基因编辑技术改良棉花的生长发育、抗逆性等性状;其次,重点评述了当前备受关注的CRISPR基因编辑系统在改良棉花抗逆性、纤维与种子品质性状等方面的应用;最后分析了基因编辑技术在应用过程中存在的不足和局限,并指出未来应进一步优化开发有知识产权的基因编辑系统,提高其精确性、安全性,促进其在棉花种质创新和遗传改良中的应用。

关键词：基因编辑棉花遗传改良 CRISPR/Cas9

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Cotton cultivation technology with Chinese characteristics has driven the 70-year development of cotton production in China

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Journal of Integrative Agriculture 2022年第3期21卷 597-609页

作者： FENG Lu CHI Bao-jie dong he-zhong Zhengzhou Research Base State Key Laboratory of Cotton Biology School of Agricultural Sciences Zhengzhou University Cotton Research Center Shandong Academy of Agricultural Sciences College of Agricultural Science and Technology Shandong Agriculture and Engineering University

Since the founding of the People’s Republic of China in 1949,significant achievements have been made in cotton production in *** has maintained its position as the world’s largest cotton producer for 33 years(1983–2015),with average annual increases of 3.5 and 3.9% in the unit yield and total output of cotton,*** production has played an extremely important role in the development of the national economy and the improvement of living *** the cotton planting area has been reduced in recent years,the total output has remained relatively unchanged due to the continuous increase in the unit ***’s dominant position in global cotton production is undoubtedly attributed to the progress and development of cotton cultivation *** the past 70 years,China has established a high-yielding and high-efficiency cotton cultivation mode that corresponds to its national conditions,including a large population and a limited land ***,cotton cultivation technology is constantly being innovated and developed to keep pace with the *** this paper,we review the development of cotton production and cultivation in China over the past 70 years,with a particular focus on the innovation and development of cotton cultivation technology with Chinese *** review is intended to provide guidance for the sustainable development of China’s cotton production in the future and to provide a reference for global cotton production.

关键词： China cotton cultivation technology outlook

Effects of chemical topping on cotton development,yield and quality in the Yellow River Valley of China

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Journal of Integrative Agriculture 2022年第1期21卷 78-90页

作者： ZHU Ling-xiao LIU Lian-tao SUN Hong-chun ZHANG Yong-jiang ZHANG Ke BAI Zhi-ying LI An-chang dong he-zhong LI Cun-dong College of Agronomy Hebei Agricultural University/State Key Laboratory of North China Crop Improvement and Regulation/Key Laboratory of Crop Growth Regulation of Hebei ProvinceBaoding 071001P.R.China Cotton Research Center Shandong Academy of Agricultural SciencesJinan 250100P.R.China

Topping is a cultivation method that is widely practiced due to the indeterminate growth character of cotton(Gossypium hirsutum L.). Among the different methods of accomplishing topping, manual topping is common in the Yellow River Valley of China, although it is time-and labor-intensive. The objective of this study was to characterize the responses of cotton to different topping treatments with respect to development, yield and quality. This study included field experiments from 2015 to 2016 with three different topping methods: manual topping(MT), chemical topping(CT) using mepiquat chloride,and a non-decapitation treatment(NT). We found that the plant height, the number of fruiting branches and the length of upper fruiting branches of cotton treated with CT were significantly lower than NT. The chlorophyll content of cotton treated with CT was not significantly different from NT, but was higher than that of MT in the later season. CT enhanced plant development with reduced endogenous gibberellic acid and abscisic acid contents, and the apical development of the main stem was inhibited. Compared with MT, CT significantly increased the biomass of the vegetative parts. Most importantly,there were no significant differences in the yield or fiber quality between MT and CT. These findings suggested that CT, a simplified and effective topping method, could be utilized as an alternative in the Yellow River Valley of China.

关键词： cotton topping development yield fiber quality

Stabilizing High-Nickel Cathodes via Interfacial Hydrogen Bonding Effects Using a Hydrofluoric Acid-Scavenging Separator

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Engineering 2024年第8期39卷 117-126页

作者： Shijie zhong Liwei dong Botao Yuan Yueyao dong Qun Li Yuanpeng Ji Yuanpeng Liu Jiecai Han Weidong he National Key Laboratory of Science and Technology on Advanced Composites in Special Environments &Center for Composite Materials and StructuresHarbin Institute of TechnologyHarbin 150080China MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage School of Chemistry and Chemical EngineeringHarbin Institute of TechnologyHarbin 150001China Chongqing Research Institute Harbin Institute of TechnologyChongqing 401151China School of Mechanical Engineering Chengdu UniversityChengdu 610106China

Nickel-rich layered Li transition metal oxides are the most promising cathode materials for high-energydensity Li-ion ***,they exhibit rapid capacity degradation induced by transition metal dissolution and structural reconstruction,which are associated with hydrofluoric acid(HF)generation from lithium hexafluorophosphate *** potential for thermal runaway during the working process poses another *** are promising components to alleviate the aforementioned ***,an ultrathin double-layered separator with a 10 lm polyimide(PI)basement and a 2 lm polyvinylidene difluoride(PVDF)coating layer is designed and fabricated by combining a nonsolvent induced phase inversion process and coating *** PI skeleton provides good stability against potential thermal shrinkage,and the strong PI-PVDF bonding endows the composite separator with robust structural integrity;these characteristics jointly contribute to the extraordinary mechanical tolerance of the separator at elevated ***,unique HF-scavenging effects are achieved with the formation of-CO…H-F hydrogen bonds for the abundant HF coordination sites provided by the imide ring;hence,the layered Ni-rich cathodes are protected from HF attack,which ultimately reduces transition metal dissolution and facilitates long-term cyclability of the Ni-rich ***||NCM811 batteries(where“NCM”indicates LiNi_(x)Co_(y)Mn_(1-x-y)O_(2))with the proposed composite separator exhibit a 90.6%capacity retention after 400 cycles at room temperature and remain sustainable at 60℃with a 91.4%capacity retention after 200 *** adopting a new perspective on separators,this study presents a feasible and promising strategy for suppressing capacity degradation and enabling the safe operation of Ni-rich cathode materials.

关键词： Nickel-rich cathodes Composite separator HF scavenging Transition metal dissolution Long-term cyclability

Control of cotton pests and diseases by intercropping:A review

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Journal of Integrative Agriculture 2021年第12期20卷 3089-3100页

作者： CHI Bao-jie ZHANG dong-mei dong he-zhong Cotton Research Center Shandong Academy of Agricultural SciencesJinan 250100P.R.China College of Life Sciences Shandong Normal UniversityJinan 250014P.R.China College of Agronomy Hebei Agricultural UniversityBaoding 071000P.R.China

Cotton(Gossypium hirsutum L.)is a globally important crop that is often damaged by pests and *** cotton pests and diseases management is dependent on chemical *** chemical pesticides are usually effective,long-term application of these pesticides often leads to increased insecticide resistance in the pests,fewer natural enemies,reduced natural control,and a degraded *** of increased environmental awareness and the need for sustainable cotton production,the control of cotton pests and diseases using biological means like intercropping is increasingly receiving *** of cotton with other crops can often boost the total yield and output of the intercropping system and provide significant economic benefits without sacrificing cotton *** also increases the number of natural enemies,and reduces the occurrence of cotton pests and diseases by altering the ecological structure and environmental conditions in the ***-based intercropping is an effective strategy to reduce the competition between cotton and grain or other economic crops for arable *** is also an important way to increase the populations of natural enemies in cotton fields for the management of pests and ***,inappropriate intercropping can also increase labor requirements and even result in inadequate control of pests and *** review focuses on the performance and the mechanisms of intercropping for reducing cotton pests and disease as well as on the effective management of intercropping *** risks and limitations,as well as the study approaches needed and the prospects of intercropping for the control of cotton pests and diseases,are also *** information is intended to aid researchers and growers in designing economically viable and ecologically friendly pest and disease management strategies that will reduce the use of chemicals and the cost of cotton production.

关键词： cotton-based intercropping pest management risk and limitation

Energy partition in laser-induced cavitation bubbles near the rigid wall with a gas-containing hole

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Journal of Hydrodynamics 2024年第3期36卷 435-443页

作者： dong-qiao he Hai-gang Wen Si-yuan Geng Chen-xi Yang Qiang zhong Zhi-feng Yao College of Water Resources and Civil Engineering China Agricultural UniversityBeijing100083China PowerChina Beijing Engineering Corporation Limited Beijing100024China State Key Laboratory of Efficient Utilization of Agricultural Water Resources Beijing100083China Beijing Engineering Research Center of Safety and Energy Saving Technology for Water Supply Network System China Agricultural UniversityBeijing100083China

To investigate the energy partition in laser-induced cavitation bubbles near the rigid wall with a gas-containing hole,we utilized a nanosecond resolution photography system based on a Q-switched Nd:YAG laser and conventional industrial camera to carefully observe the transient process of bubble collapse near the rigid wall with a gas-containing *** analyzed the generation of collapse microjets and the emission of collapse shock *** found that the cavitation bubble near the rigid wall with a gas-containing hole collapsed at different times and space,and produced various types of shock *** on the far field pressure information of the shock waves measured by hydrophone,the energy of the shock waves generated by the bubble collapse near the rigid wall with a gas-containing hole is calculated for the first *** results show that the ratio of collapse shock wave energy to bubble energy is approximately between 0.7 and 0.8.

关键词： Energy partition bubble collapse laser-induced shock wave gas-containing hole

Multistimuli-Responsive Luminescent Porous Organic Polymers with Chiroptical Properties and Acid-Induced Degradation

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Chinese Journal of Chemistry 2024年第16期42卷 1886-1894页

作者： Youling he dongyang Fan Liang Zhang dong Wang Ting Han Rongrong Hu Ben zhong Tang Center for AIE Research Shenzhen Key Laboratory of Polymer Science and TechnologyGuangdong Research Center for Interfacial Engineering of Functional MaterialsCollege of Materials Science and EngineeringShenzhen UniversityShenzhenGuangdong 518060China College of Physics and Optoelectronic Engineering Shenzhen UniversityShenzhenGuangdong 518060China Department of Chemistry Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and ReconstructionThe Hong Kong University of Science and TechnologyClear Water BayKowloonHong Kong999077China State Key Laboratory of Luminescent Materials and Devices Guangdong Provincial Key Laboratory of Luminescence from Molecular AggregatesSouth China Universityof TechnologyGuangzhouGuangdong 510640China School of Science and Engineering Shenzhen Institute of Aggregate Science and TechnologyThe Chinese University of Hong KongShenzhen(CUHK-Shenzhen)Guangdong 518172China

Porous organic polymers(POPs)have attracted great attention in past *** diverse functional POPs have been developed,multistimuli-responsive POPs with excellent aggregate-state luminescence together with good chiroptical properties have rarely been ***,two pairs of Salen-type enantiomeric PoPs with multistimuli-responsive luminescence and chiral features were designed and synthesized by facile polycondensation reactions between polyfunctional aggregation-induced emission luminogen(AlEgen)-containing salicylaldehyde derivatives and chiral *** Salen units in polymer backbones as tetradentate ligands,a series of POP-metal complexes were further *** obtained POPs and metal complexes show good porosity,high thermal stability,and obvious circular dichroism ***,benefiting from the coexistence of AlEgen and Salen units in polymer structures,these POPs exhibit excellent luminescence performance in aggregate states and tunable fluorescence behaviors in response to external stimuli of Zn^(2+)ion,mechanical forces,organic solvent,and *** to the dynamic feature of Schiff base C=N bonds,the present POPs can efficiently undergo hydrolysis reactions under strong acidic conditions to reproduce the AlEgencontaining monomers,and such an acid-induced degradation process can be directly visualized and dynamically monitored via fluorescence *** properties collectively make the POPs candidate materials for applications in heterogeneous asymmetric catalysis,fluorescence sensing,biomedicine,etc.

关键词： Polymers Stimuli-responsive luminescence Porous polymers Chirality Aggregation-induced emission Fluorescence Schiff bases Degradation

New Results in Cooperative Adaptive Optimal Output Regulation

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Journal of Systems Science & Complexity 2024年第1期37卷 253-272页

作者： dong Yuchen GAO Weinan JIANG zhong-Ping State Key Laboratory of Synthetical Automation for Process Industries Northeastern UniversityShenyang 110819China Department of Electrical and Computer Engineering Tandon School of EngineeringNew York UniversityBrooklynNY 11201USA

This paper investigates the cooperative adaptive optimal output regulation problem of continuous-time linear multi-agent *** the multi-agent system dynamics are uncertain,solving regulator equations and the corresponding algebraic Riccati equations is challenging,especially for high-order *** this paper,a novel method is proposed to approximate the solution of regulator equations,i.e.,gradient descent *** is worth noting that this method obtains gradients through online data rather than model information.A data-driven distributed adaptive suboptimal controller is developed by adaptive dynamic programming,so that each follower can achieve asymptotic tracking and disturbance ***,the effectiveness of the proposed control method is validated by simulations.

关键词： Adaptive dynamic programming cooperative output regulation gradient descent method multi-agent systems.

Syrosingopine,an anti-hypertensive drug and lactate transporter(MCT1/4)inhibitor,activates hepatic stellate cells and exacerbates liver fibrosis in a mouse model

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Genes & Diseases 2024年第4期11卷 28-30页

作者： Meichun Guo Yannian Gou Xiangyu dong Jiamin zhong Aohua Li Ailing Hao Tongchuan he Jiaming Fan Ministry of Education Key Laboratory of Diagnostic Medicine and the School of Laboratory Diagnostic MedicineChongqing Medical UniversityChongqing 400016China Molecular Oncology Laboratory Department of Orthopaedic Surgery and Rehabilitation MedicineThe University of Chicago Medical CenterChicagoIL 60637USA

Syrosingopine is an anti-hypertensive drug and can cause high intracellular lactate levels and end-product inhibition of lactate dehydrogenase by inhibiting the lactate transporters MCT1 and *** studies have shown that syrosingopine plays an essential role in the process of glycolytic blockade,ATP depletion,and cell death in cancer due to high intracellular levels of lactate.

关键词： hypertensive MCT1 drug