检索结果-南通市图书馆

Advanced technologies for single-cell in situ protein profiling

在线全文

学校读者我要写书评

暂无评论

Science China Chemistry 2022年第1期65卷 48-67页

作者： Wen Zhou Xiaoyue Ni Chen Xie Quli Fan dingbin Liu State Key Laboratory of Organic Electronics and Information Displays and Jiangsu National Synergetic Innovation Center for Advanced Materials(SICAM)Institute of Advanced Materials(IAM)Nanjing University of Posts&TelecommunicationsNanjing 210023China State Key Laboratory of Medicinal Chemical Biology Research Center for Analytical SciencesTianjin Key Laboratory of Biosensing and Molecular RecognitionCollege of ChemistryNankai UniversityTianjin 300071China

Cells are inherently heterogeneous to achieve a diverse spectrum of biological *** understand the underlying protein machinery that achieves these fascinating functions,it is important to develop advanced analytical methods that can profile proteins in their native environment,as protein expression,aggregation,degradation,and regulation define both normal physiology as well as *** and transcriptome sequencing have seen major advances at the single-cell levels,but comprehensive proteomic profiling is still *** conventional proteomic methods,such as enzyme-linked immunosorbent assay(ELISA),western blot,and protein chips,can characterize biomarkers of ***,these ensemble techniques are unsuitable for single-cell *** evidence has shown the significance of in situ,sensitive,quantitative,and multiplexed profiling of biomarkers in single cells for diagnosis and treatment ***,we review the recent development of advanced imaging and spectroscopy techniques,including mass cytometry,immunofluorescence,and surfaceenhanced Raman spectrometry(SERS)for single-cell proteomic *** also provide our view on the challenges and the outlook.

关键词： proteomics single-cell CyTOF immunofluorescence SERS

在线全文

学校读者我要写书评

暂无评论

大型四足机器人腿部结构设计与运动分析

机械设计与研究 2024年第3期40卷 46-50页

作者：梅贤慧张文典黄家才张杨桂孙丁斌南京工程学院机械工程学院南京211167 江苏省仿生控制技术与装备工程研究中心南京211167 长春汽车工业高等专科学校电气工程学院长春130013

基于大型四足机器人在行走时出现的运动稳定性不足问题,设计了一种基于5杆机构的串联腿部结构,该结构具有结构简单、运动精度高、承载能力强的特点。建立了单腿简化结构模型,利用CoppeliaSim与matlab搭建的联合仿真平台对结构进行运动... 详细信息

基于大型四足机器人在行走时出现的运动稳定性不足问题,设计了一种基于5杆机构的串联腿部结构,该结构具有结构简单、运动精度高、承载能力强的特点。建立了单腿简化结构模型,利用CoppeliaSim与matlab搭建的联合仿真平台对结构进行运动学分析、步态分析和足端轨迹规划,通过分析大腿与小腿关节的位移、角速度的变化特点并与样机反馈数据进行比对。结果表明,该结构在所设计的步态与足端轨迹下运动时,满足四足机器人在行走时对稳定性的要求,使得大型四足机器人在行走时具有更好得运动稳定性。

关键词：四足机器人运动学分析步态分析轨迹规划运动稳定性

2019-2021年海南省海口市45-74岁城市居民肺癌筛查结果及成本-效果分析

在线全文

学校读者我要写书评

暂无评论

中国肿瘤 2024年第6期33卷 492-497页

作者：张旭华婧范康琼王定彬苏良苹刘韦淞田冶海南省肿瘤防治中心肿瘤登记及早诊早治办公室海南海口570100 海南医学院公共卫生与全健康国际学院海南海口571199

[目的]分析2019-2021年海口市城市居民肺癌筛查项目筛查结果和成本效果,为我国城市人群癌症筛查和相关卫生决策提供多角度参考依据。[方法]基于2019-2021年海口市肺癌筛查项目数据及成本资料,分析45~74岁城市居民肺癌高危率、依从性和... 详细信息

[目的]分析2019-2021年海口市城市居民肺癌筛查项目筛查结果和成本效果,为我国城市人群癌症筛查和相关卫生决策提供多角度参考依据。[方法]基于2019-2021年海口市肺癌筛查项目数据及成本资料,分析45~74岁城市居民肺癌高危率、依从性和病变检出率;测算直接成本、间接成本及疑似肺癌的早期发现成本系数(EDCI)等指标,评价肺癌筛查的成本效果。[结果]2019-2021年共31058人参与海口市高危风险评估问卷初筛,其中11594人评估为肺癌高危人群,高危率为37.33%,高危人群中7164人接受低剂量螺旋CT(LDCT)检查,依从率为61.79%。检出肺阳性结节372例(检出率为5.19%),疑似肺癌77例(检出率为1.07%)。2019-2021年肺癌筛查的总费用为2820868元,每例肺癌的平均筛查费用为393.76元。45~74岁居民中每检出1例肺阳性结节费用为7582.98元,每检出1例疑似肺癌的费用为36634.65元,EDCI为0.48;各年龄组中65~74岁组检出1例疑似肺癌的费用最低(24376.21元),其EDCI为0.32。[结论]LDCT筛查肺癌高危人群具有良好的成本效用价值,尤其在65~74岁年龄组更具筛查价值。

关键词：肺癌筛查低剂量螺旋CT成本-效果分析

In-situ monitoring of nitrile-bearing pesticide residues by background-free surface-enhanced Raman spectroscopy

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2022年第1期33卷 519-522页

作者： Guorui Wu Wenshuai Li Weijun Du Aiqin Yue Jinzhong Zhao dingbin Liu College of Agronomy Shanxi Agricultural UniversityTaigu 030801China College of Arts and Sciences Shanxi Agricultural UniversityTaigu 030801China State Key Laboratory of Medicinal Chemical Biology Research Center for Analytical Sciencesand Tianjin Key Laboratory of Biosensing and Molecular RecognitionCollege of ChemistryNankai UniversityTianjin 300071China

In-situ monitoring of pesticide residues during crop growth or/and in related products is of great significance in avoiding the abuse of pesticides but remains challenging thus *** this report,we proposed a background-free surface-enhanced Raman spectroscopy(bf-SERS)platform to non-destructively track the nitrile-bearing pesticide residues in soybean leaves with high sensitivity and *** outstanding feature of the assay stems from the dramatic Raman enhancement effect of the 50 nm-sized gold nanoparticles(AuNPs)towards the pesticides and simultaneously the background-free Raman signal of the nitrile group in the so-called Raman-silent region(1800-2800 cm^(-1)).This bf-SERS assay was applied to evaluate the penetration effects of nitrile-bearing pesticides and monitor their residues in soybean leaves after rinsing with various solutions,providing a reliable tool for guiding the safe use of nitrile-bearing pesticides in agriculture.

关键词： Nitrile-bearing pesticide residues Background-free SERS In-situ detection Chemical sensing Food safety

在线全文

学校读者我要写书评

暂无评论

强震作用下特高拱坝动力响应及损伤特征研究

工程地球物理学报 2024年第1期21卷 22-34页

作者：高志良黄会宝沈定斌彭涛国能大渡河流域水电开发有限公司四川成都610041 四川大学水利水电学院四川成都610065

我国的特高拱坝多建于西南地区,西南地区处于地震多发区,且震级大、烈度高,因此特高拱坝的抗震安全一直是重点关注问题。本文以位于强震区的大岗山特高拱坝为研究对象,采用真实破裂过程分析软件RFPA 3D-Dynamics开展地震动力响应数值模... 详细信息

我国的特高拱坝多建于西南地区,西南地区处于地震多发区,且震级大、烈度高,因此特高拱坝的抗震安全一直是重点关注问题。本文以位于强震区的大岗山特高拱坝为研究对象,采用真实破裂过程分析软件RFPA 3D-Dynamics开展地震动力响应数值模拟。研究了地震作用下坝体变形、应力变化规律,根据损伤演化及声发射变化规律提出大岗山拱坝地震作用下的潜在失稳损伤区域。研究结果表明:拱坝坝体内部随地震波扰动发生局部应力集中、应力释放现象,拱坝在设计地震波(地震峰值加速度0.5575 g)作用下能够依靠自身结构调整,保持变形协调,应力分布合理,无大规模损伤破坏。在1.5倍设计地震波情况下,拱坝顺河向位移突变量超过240 mm,远超坝体正常位移变形范围,此时拱坝发生大规模损伤开裂现象,且主要损伤开裂区域为拱冠梁顶部及左右半拱中上部区域。通过研究拱坝地震荷载作用下的动态响应特征,分析损伤演化规律,能够较为直观地判断拱坝易受损区域和掌握拱坝工作性态。

关键词：拱坝地震动力响应坝体损伤

维普期刊数据库博看期刊

Peripheral origin exosomal micro RNAs aggravate glymphatic system dysfunction in diabetic cognitive impairment

在线全文

学校读者我要写书评

暂无评论

药学学报:英文版 2023年第7期13卷 2817-2825页

作者： Lin Zhang Dongna Li Pengrong Yi Jiangwei Shi Mengqing Guo Qingsheng Yin dingbin Liu Pengwei Zhuang Yanjun Zhang State Key Laboratory of Component-based Chinese Medicine Tianjin University of Traditional Chinese MedicineTianjin 301617China Haihe Laboratory of Modern Chinese Medicine Tianjin 301617China First Teaching Hospital of Tianjin University of Traditional Chinese Medicine Tianjin 300193China National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion Tianjin 300193China State Key Laboratory of Medicinal Chemical Biology Research Center for Analytical Sciencesand Tianjin Key Laboratory of Molecular Recognition and BiosensingCollege of ChemistryNankai UniversityTianjin 300071China

Cognitive dysfunction is one of the common central nervous systems(CNS)complications of diabetes mellitus,which seriously affects the quality of life of patients and results in a huge economic *** glymphatic system dysfunction mediated by aquaporin-4(AQP4)loss or redistribution in perivascular astrocyte endfeet plays a crucial role in diabetes-induced cognitive impairment(DCI).However,the mechanism of AQP4 loss or redistribution in the diabetic states remains *** evidence suggests that peripheral insulin resistance target tissues and CNS communication affect brain homeostasis and that exosomal miRNAs are key *** and lipid metabolism disorder is an important pathological feature of diabetes mellitus,and skeletal muscle,liver and adipose tissue are the key target insulin resistance *** this review,the changes in exosomal miRNAs induced by peripheral metabolism disorders in diabetes mellitus were systematically *** focused on exosomal miRNAs that could induce low AQP4 expression and redistribution in perivascular astrocyte endfeet,which could provide an interorgan communication pathway to illustrate the pathogenesis of ***,the mechanisms of exosome secretion from peripheral insulin resistance target tissue and absorption to the CNS were summarized,which will be beneficial for proposing novel and feasible strategies to optimize DCI prevention and/or treatment in diabetic patients.

关键词： Diabetic cognitive impairment AQP4 Peripheral-central communication Exosomal miRNAs Central nervous system Glymphatic system Diabetes mellitus Astrocyte

Synthesis and Crystal Structure of Copper(Ⅱ) Compound with Didentate Schiff-base Ligand

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Structural Chemistry 2004年第1期23卷 25-28页

作者： YILong dingbin CHENGPeng DepartmentofChemistry NankaiUniversityTianjin300071China DepartmentofChemistry NankaiUniversityTianjin300071ChinaTel：022-23509957Fax：022-23502458E-mail：pcheng@***

The title complex [Cu(L)2] (HL=4-salicylideneamino-3,5-dimethanol-1,2,4-triazole) was synthesized by the reaction of Cu(CH3COO)22H2O with 4-salicylideneamino-3,5-dimethanol- 1,2,4-triazole in the methanol. The single-crystal X-ray study reveals that the compound crystallizes in monoclinic, space group P21/n with a = 11.746(5), b = 8.145(3), c = 12.786(5) ? b = 106.64(1), V = 1172(1) ?, Z = 2, Dc = 1.581 g/cm3, m = 0.989 mm-1, F(000) = 574, C22H22Cu- N8O6, Mr = 558.02, T = 293(2) K, R = 0.0394 and Rw = 0.0835 for 2376 observed reflections with I > 2s(I). The complex comprises a four-coordinated copper(II) center, with an N2O2 planar coordi- nation environment. The molecules are connected by hydrogen bonds to form one-dimensional chains which are further linked to form a two-dimensional plane.

关键词： crystal structure copper(Ⅱ) complex hydrogen bond

117例肺泡灌洗液阳性老年下呼吸道感染患者病原菌分布及耐药性分析

在线全文

学校读者我要写书评

暂无评论

检验医学与临床 2024年第12期21卷 1806-1810页

作者：夏珺李泉张吉曾建涛吴永琴刘钉宾重庆市长寿区人民医院检验科重庆401220

目的分析肺泡灌洗液(BALF)阳性的老年下呼吸道感染患者相关病原菌的分布和耐药性,为老年下呼吸道感染的预防及合理用药提供依据。方法对该院2020年1月1日至2023年6月30日送检的高度怀疑下呼吸道感染的664例住院老年患者BALF标本进行培养... 详细信息

目的分析肺泡灌洗液(BALF)阳性的老年下呼吸道感染患者相关病原菌的分布和耐药性,为老年下呼吸道感染的预防及合理用药提供依据。方法对该院2020年1月1日至2023年6月30日送检的高度怀疑下呼吸道感染的664例住院老年患者BALF标本进行培养,采用微量肉汤稀释法进行药敏试验。结果BALF标本中分离出病原菌147株,BALF标本阳性率为22.1%(147/664)。排除来自同一患者的重复分离菌株后,分离的病原菌为117株。117株病原菌中革兰阳性菌占7.7%,革兰阴性菌占72.6%,真菌占19.7%。其中革兰阴性菌以鲍曼不动杆菌、铜绿假单胞菌和肺炎克雷伯菌为主;革兰阳性菌以肺炎链球菌、金黄色葡萄球菌为主;真菌以白色假丝酵母菌为主。分离出117株病原菌的下呼吸道感染患者中,医院获得性肺炎46例(39.3%),社区获得性肺炎71例(60.7%)。药敏试验结果显示:鲍曼不动杆菌对呋喃妥因和头孢唑啉耐药率最高,为100.0%,对亚胺培南、美罗培南耐药率均为84.0%,对多黏菌素B、米诺环素、头孢噻肟耐药率均为0.0%;铜绿假单胞菌对氨苄西林/舒巴坦耐药率最高,为85.7%,对复方磺胺甲噁唑、氯霉素、米诺环素耐药率分别为64.3%、78.6%、45.5%;肺炎克雷伯菌除对氨苄西林天然耐药外,对其他测试药物的耐药率在0.0%~35.3%;白色假丝酵母菌对伏立康唑、两性霉素B均敏感。结论老年下呼吸道感染患者BALF标本检出病原菌以革兰阴性菌为主,其中鲍曼不动杆菌的耐药情况较严重,真菌感染也应该引起重视。

关键词：下呼吸道感染肺泡灌洗液病原菌耐药性老年患者

同方期刊数据库

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

肛周乳头状汗腺腺瘤

临床皮肤科杂志 2024年第4期53卷 193-194页

作者：杨定彬毛玉戚欣雨郭梦露朱仁衡彭露杨学军任林黄淑琼乐山市人民医院皮肤科四川乐山614000 乐山市人民医院病理科四川乐山614000

患者女,38岁主诉:肛周左侧淡红色结节10年。现病史:患者10年前无明显诱因肛门左侧出现一绿豆大淡红色结节,无痛痒,未予诊治。皮损一直未消退,曾自行外用痔疮膏治疗后皮损未缓解,逐渐增大至花生米大结节。为明确诊断,遂于2022年9月13日... 详细信息

患者女,38岁主诉:肛周左侧淡红色结节10年。现病史:患者10年前无明显诱因肛门左侧出现一绿豆大淡红色结节,无痛痒,未予诊治。皮损一直未消退,曾自行外用痔疮膏治疗后皮损未缓解,逐渐增大至花生米大结节。为明确诊断,遂于2022年9月13日来我院皮肤科就诊。

关键词：汗腺腺瘤,乳头状肛周