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Next Generation Transcriptome Sequencing and Quantitative Real-Time PCR Technologies for Characterisation of the Bemisia tabaci Asia 1 mtCOI Phylogenetic Clade

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Journal of Integrative Agriculture 2012年第2期11卷 281-292页

作者： Susan Seal Mitulkumar V Patel Carl Collins John Colvin david Bailey Natural Resources Institute University of GreenwichChatham Maritime Kent ME4 4TBUnited Kingdom

A programme of functional genomics research is underway at the University of Greenwich,UK,to develop and apply genomics technologies to characterise an economically-important but under-researched Bemisia tabaci（Hemiptera：Aleyrodidae）,the Asia 1 mtCOI phylogenetic group.A comparison of this putative species from India with other important *** populations and insect species may provide targets for the development of more effective whitefly control *** a first step,next-generation sequencing（NGS）has been used to survey the transcriptome of adult female whitefly,with high quality RNA preparations being used to generate cDNA libraries for NGS using the Roche 454 Titanium DNA sequencing *** assemblies constructed from the resultant sequences（301 094 reads）using the software program CLC Genomics Workbench generated 3 821 core *** of a selection of these contigs with related sequences from other *** genetic groups has revealed good alignment for some genes（e.g.,HSP90）but misassemblies in other datasets（e.g.,the vitellogenin gene family）,highlighting the need for manual curation as well as collaborative international efforts to obtain accurate assemblies from the existing next generation sequence ***,data emerging from the NGS has facilitated the development of accurate and reliable methods for analysing gene expression based on quantitative real-time RT-PCR,illustrating the power of this approach to enable rapid expression analyses in an organism for which a complete genome sequence is currently lacking.

关键词： Bemisia tabaci whitefly transcriptome next generation sequencing quantitative real-time (QRT)-PCR Asia 1 mtCOI

Assessment of the predictive capabilities of NIGTEC model over Nigeria during geomagnetic storms

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Geodesy and Geodynamics 2021年第6期12卷 413-423页

作者： Paul Obiakara Amaechi Ibifubara Humphrey david Adeyinka Adewoyin Department of Physical Sciences Chrisland UniversityOwodeAbeokutaOgun StateNigeria Department of Physics University of LagosLagosNigeria

The Nigerian Total Electron Content(NIGTEC)is a regional neural netwo rk-based model developed by the Nigerian Centre for Atmospheric Research to predict the Total Electron Content(TEC)at any location over *** addition of the disturbance storm time(Dst)index as one of NIGTEC’s input layer neurons raises a question of its accuracy during geomagnetic *** this paper,the capability of NIGTEC in predicting the variability of TEC during geomagnetic storms has been *** data predicted by NIGTEC is compared with those derived from Global Navigation Satellite System(GNSS)over Lagos(6.5°N,3.4°E)and Toro(10.1°N,9.120 E)during the intense storms in March 2012 and *** model’s predictive capability is evaluated in terms of Root Mean Square Error(RMSE).NIGTEC reproduced a fairly good storm time morphology in VTEC driven by the prompt penetration electric field and the increase in thermospheric O/***,it failed to predict the increase in TEC after the intense sudden impulse of 60 nT on 8 March *** it could not capture the changes in VTEC driven by the storm time equatorward neutral wind especially during 18:00-24:00 ***,the RMSEs were higher during this time window,and the highest RMSE value was obtained during the most intense storm in March 2012.

关键词： Global navigation satellite system Total electron content Geomagnetic storm Nigerian Total Electron Content(NIGTEC)

Male sexual signaling and expected effects of hatchery-induced sperm competition vary with water depth at which whitefish are caught

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Current Zoology 2021年第3期67卷 337-340页

作者： Giulia Perroud david Nusbaumer Christian de Guttry Claus Wedekind Department of Ecology and Evolution University of Lausanne1015 LausanneSwitzerland

Salmonids like whitefish(Coregonus spp.)are often propagated in supportive *** are caught from their spawning locations,their gametes mixed,and the resulting offspring reared in a protected environment before being released into the *** procedure can affect sexual selection,for example,by enhancing the importance of sperm competition or by reducing the relevance of sexual *** it is often unclear how sperm competitiveness is affected by a male's overall genetic quality,there is accumulating evidence that sexual signals reveal good genes and that mate choice based on such signals can increase offspring viability(Auld et al.2019).Therefore,supportive breeding may affect the genetic variance and the mean genetic quality of next generations.

关键词： sexual signaling sperm competition whitefish

Evaluation of different crosslinking methods in altering the properties of extrusion-printed chitosan-basedmulti-material hydrogel composites

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Bio-Design and Manufacturing 2023年第2期6卷 150-173页

作者： Suihong Liu Haiguang Zhang Tilman Ahlfeld david Kilian Yakui Liu Michael Gelinsky Qingxi Hu Centre for Translational Bone Joint and Soft Tissue ResearchFaculty of Medicine and University Hospital Carl Gustav CarusTechnische Universität Dresden01307 DresdenGermany Rapid Manufacturing Engineering Center School of Mechatronic Engineering and AutomationShanghai UniversityShanghai 200444China Shanghai Key Laboratory of Intelligent Manufacturing and Robotics School of Mechatronic Engineering and AutomationShanghai UniversityShanghai 200444China National Demonstration Center for Experimental Engineering Training Education Shanghai UniversityShanghai 200444China

Three-dimensional printing technologies exhibit tremendous potential in the advancing fields of tissue engineering and regenerative medicine due to the precise spatial control over depositing the *** their widespread utilization and numerous advantages,the development of suitable novel biomaterials for extrusion-based 3D printing of scaffolds that support cell attachment,proliferation,and vascularization remains a ***-material composite hydrogels present incredible potential in this ***,in this work,a multi-material composite hydrogel with a promising formulation of chitosan/gelatin functionalized with egg white was developed,which provides good printability and shape *** addition,a series of comparative analyses of different crosslinking agents and processes based on tripolyphosphate(TPP),genipin(GP),and glutaraldehyde(GTA)were investigated and compared to select the ideal crosslinking strategy to enhance the physicochemical and biological properties of the fabricated *** of the results indicate that the composite hydrogel and the resulting scaffolds utilizing TPP crosslinking have great potential in tissue engineering,especially for supporting neo-vessel growth into the scaffold and promoting angiogenesis within engineered tissues.

关键词： Multi-material composite hydrogel Crosslinking mechanism Chitosan Gelatin Egg white 3D printing

A good gut feeling on Sarm1-mediated axon degeneration

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Protein & Cell 2021年第8期12卷 596-598页

作者： Victoria E.von Saucken david J.Simon Department of Biochemistry Weill Cornell Medical College Tri-Institutional MD-Ph D Program Weill Cornell Medicine Rockefeller University Memorial Sloan Kettering Cancer Center

Axons,the cable-l ike projections of neurons,experience a wide range of stressors throughout their *** result of any one of these challenges can be catastrophic,promoting degeneration of the *** degeneration is an active signaling process observed in traumatic brain injury,chemotherapy-induced peripheral neuropathies,and neurodegen erative disease (Simon and Watkins,2018;Coleman and Hoeke,2020).As a result,tremendous efforts have been made to identify biological targets that can be harnessed to block axon degeneration to treat these *** promising candidate is Sarm1 (sterile alpha and TIR motif containing protein 1),a member of the Myd88 immune adaptor protein family that itself has no clear role in innate immunity.

关键词： Biochemistry general Protein Science Cell Biology Stem Cells Human Genetics Developmental Biology

Gate length dependent transport properties of in-plane core-shell nanowires with raised contacts

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Nano Research 2020年第1期13卷 61-66页

作者： Alexandre Bucamp Christophe Coinon david Troadec Sylvie Lepilliet Gilles Patriarche Xavier Wallart Ludovic Desplanque Univ.Lille CNRSCentrale LilleYncréa ISENUniv.Polytechnique Hauts-de-FranceUMR 8520-IEMNF-59000LilleFrance C2N-UMR 9001 CNRS UniversitéParis-Sud-UniversitéParis-Saclay91120PalaiseauFrance

Three-dimensional(3D)nanoscale crystal shaping has become essential for the precise design of advanced electronic and quantum devices based on electrically gated *** this context,Ⅲ-Ⅴ semiconductor-based nanowires with low electron effective mass and strong spin-orbit coupling are particularly investigated because of their exceptional quantum transport properties and the good electrostatic control they *** the main challenges involved in the processing of these nanodevices are(i)the management of the gate stack which requires ex-situ passivation treatment to reduce the density of traps at the oxide/semiconductor interface,(ii)the ability to get good ohmic contacts for source and drain electrodes and(iii)the scalability and reliability of the process for the fabrication of complex architectures based on nanowire *** this paper,we show that selective area molecular beam epitaxy of in-plane InGaAs/InP core-shell nanowires with raised heavily doped source and drain contacts can address these different *** characterization of the devices down to 4 K reveals the positive impact of the InP shell on the gate electrostatic control and effective electron *** comparable to the best reported values for In(Ga)As nanostructures grown on InP,this latter is severely reduced for sub-100 nm channel highlighting remaining issue to reach the ballistic regime.

关键词： molecular beam epitaxy core-shell nanowire selective area growth effective electron mobility

A NEW TWO-WAY AUTHENTICATION AND PRIVACY MECHANISM FOR WLAN SECURITY

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Journal of Pharmaceutical Analysis 2003年第2期15卷 111-115页

作者：郑庆华 david Pepyne Computer Department of Xi an Jiaotong University

Objective Aiming at lots of vulnerabilities in the Wired Equivalent Privacy (WEP) which threats the authentication and confidentiality in wireless communication, a new kind of mutual authentication and privacy mechanism named MWEP(Modified WEP) is proposed. Methods MWEP is based on pseudo random number generator (PRNG) and asymmetric cryptograph approach, it generates a unique session key like “One Time Password” for each data frame transmission between any two mobile stations. Results Using this session key to encrypt the transmission data, not only can it avoid replay attack, but also provide a good secure virtual channel for the sender and receiver. MWEP can be incorporated into IEEE 802.11. Conclusion It shows that the proposed mechanism is effective and practical after comparison with WEP and simulation.

关键词： wireless LAN security, authentication privacy WEP PRNG session key

Identification of phytochemical compounds in Calophyllum inophyllum leaves

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Asian Pacific Journal of Tropical Biomedicine 2017年第9期7卷 773-781页

作者： david Febrilliant Susanto Hakun Wirawasista Aparamarta Arief Widjaja Setiyo Gunawan Department of Chemical Engineering Institut Teknologi Sepuluh NopemberSurabaya 60111Indonesia

Objectives: To investigate the proximate composition, mineral content, and phytochemical compounds in Calophyllum inophyllum(C. inophyllum) leaves. Moreover,isolation and identification of pyrene were also ***: C. inophyllum leaves were extracted with methanol by percolation *** proximate composition of C. inophyllum leaves was analyzed by standard *** contents in this plant were analyzed by using atomic absorption spectrophotometer. Phytochemical screening and analysis of this plant were performed by spectrophotometric method. Washing method with carbon disulfide was used for isolating dihydropyrene compound from C. inophyllum leaves ***: The result revealed that C. inophyllum leaves contained 11.24% moisture,4.75% ash, 6.43% crude protein, 23.96% crude fiber, 9.91% carbohydrate, and energy(79.17 kcal/100 g). The leaves also contained 0.007% iron, 1.240% calcium, 0.075%sodium, 0.195% magnesium, 0.100% ppm potassium, and 0.040% phosphorus. Moreover, 11.51% alkaloid, 2.48% triterpenoid, 2.37% flavonoid, 7.68% tannin, 2.16%saponin, 2.53% polyphenol, were identified in the methanolic crude extracts of C. inophyllum leaves. It was found that trans-2-[2-(trifluoromethyl)phenyl]-10 b,10 c-dimethyl-10 b,10 c-dihydropyrene was obtained at purity of 79.18%(22.17% yield)from C. inophyllum ***: C. inophyllum leaves may be used as a good source of fiber. It was found that C. inophyllum leaves have the potential as herbal drugs due to their phytochemical content. The separation, isolation, and purification of bioactive compounds from this methanolic crude extract and their biological activity are under further investigation.

关键词： Calophyllum inophyllum leaves Crude extracts Proximate composition Mineral composition Phytochemical

Evaluating the impact of auxiliary fan practices on localised subsurface ventilation

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International Journal of Mining Science and Technology 2019年第6期29卷 933-941页

作者： david J.de Villiers Marc J.Mathews Philip Maré Marius Kleingeld Deon Arndt Centre for Research and Continued Engineering Development North-West University

Mines are continually expanding in size and depth,leading to an increased reliance on localised subsurface ventilation *** use of underground auxiliary fans is a favoured method to increase and control airflow in working ***,the effectiveness of auxiliary fans in this regard is not *** paper evaluated the performance of these underground fan systems in four different South African deeplevel gold mines.A total auxiliary fan system efficiency of 5%was found across six systems,with the average fan efficiency of 33 fans at 38%.The results showed that these fans deviate significantly from their design operating ***,there are significant shortcomings in current underground fan *** detailed investigations led to the conclusion that the assemblage of underground auxiliary fan systems results in significant energy ***,maintaining good underground fan practice such as optimal fan selection,ducting design and maintenance is crucial for the efficacy of a mine ventilation network.

关键词： Mine ventilation Underground fan assemblage Fan system performance