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自交系BU-21/3——甜瓜(Cucumis meloL.)转基因育种的有效工具

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中国西瓜甜瓜 2003年第4期 26-27页

作者： M.Galperin L.Patlis A.Ovdia d.wolf A.Zelcer d.Keingsbuch 李名旺龙茂华朱宗河孔令传以色列拉马特甘巴尔宜兰大学生命科学学院以色列贝特达干农业研究院植物遗传系安徽省农作物新品种引育中心合肥230051

甜瓜转化率低的问题仍具有挑战性。用1个新特质甜瓜自交系BU-21/3作材料研究其再生和转化能力,表明这个自交系的再生能力优于以往研究过的基因型。用含有报告基因GUS或GFP的根癌农杆菌浸染子叶外植体,研究瞬时表达和稳定表达效率,观察... 详细信息

甜瓜转化率低的问题仍具有挑战性。用1个新特质甜瓜自交系BU-21/3作材料研究其再生和转化能力,表明这个自交系的再生能力优于以往研究过的基因型。用含有报告基因GUS或GFP的根癌农杆菌浸染子叶外植体,研究瞬时表达和稳定表达效率,观察到了大量瞬时表达,稳定表达时每个外植体可产生0.4～1.5个转基因芽,在转化后的8～10周,将转基因植株移入可控温室,发现表型和育性正常。再生能力的遗传分析表明它受单个显性位点控制,因此,这种新特质的基因型有望成为甜瓜转基因育种的有效工具。

关键词：甜瓜转基因育种自交系BU-21/3 再生能力报告基因根癌农杆菌

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Flupirtine对癌症的镇痛功能

药学研究 1986年第2期 36-36页

作者： W.Scheef d.wolf-Gruber 王卫

在双育临床试验中,用100mg/胶囊的Flupirtine与50mg/胶囊的镇痛新做对照试验,检验Flupirtine的药效性和安全性。试验对象为52名严重的癌症患者,试验周期为七天,每天给药6个胶囊。实验程序可分4个等级进行。

关键词：镇痛新戊唑辛解热镇痛药 Flupirtine 癌症患者

data-driven prediction of plate velocities and plate deformation of explosive reactive armor

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defence Technology（防务技术） 2022年第12期18卷 2141-2149页

作者： Marvin Becker Andreas Klavzar Thomas wolf Melissa Renck Institute of Saint-Louis 5 Rue du Général Cassagnou68330Saint LouisCedexFrance Ecole Centrale de Lille CitéScientifique59651Villeneuve d'AscqCedexFrance

Explosive reactive armor(ERA)is currently being actively developed as a protective system for mobile devices against ballistic threats such as kinetic energy penetrators and shaped-charge *** mobility,the aim is to design a protection system with a minimal amount of required *** efficiency of an ERA is sensitive to the impact position and the timing of the ***,different designs have to be tested for several impact scenarios to identify the best *** analytical models are not predicting the behavior of the ERA accurately enough and experiments,as well as numerical simulations,are too time-consuming,a data-driven model to estimate the displacements and deformation of plates of an ERA system is proposed *** ground truth for the artificial neural network(ANN)is numerical simulation results that are validated with *** ANN approximates the plate positions for different materials,plate sizes,and detonation point positions with sufficient accuracy in *** a future investigation,the results from the model can be used to estimate the interaction of the ERA with a given ***,a measure for the effectiveness of an ERA can be ***,an optimal ERA can be designed and analyzed for any possible impact scenario in negligible time.

关键词： Artificial neural network Explosive reactive armor Finite element simulation Particle simulation Flash X-ray

A GIS tool for infinite slope stability analysis(GIS-TISSA)

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Geoscience Frontiers 2021年第2期12卷 756-768页

作者： Rüdiger Escobar-wolf Jonathon d.Sanders C.L.Vishnu Thomas Oommen K.S.Sajinkumar department of Geological and Mining Engineering and Sciences Michigan Technological UniversityHoughtonMIUSA department of Geology University of KeralaThiruvananthapuram 695581KeralaIndia

Landslides are one of the most common and a destructive natural hazard in mountainous terrain and thus evaluating their potential locations and the conditions under which they may occur is crucial for their hazard *** landslide occurrence in soil and regolith covered slopes are often modeled using the infinite slope model,which characterizes the slope stability in terms of a factor of safety(FS) *** approaches have been followed to also assess and propagate uncertainty through such ***(2004) introduced the use of the First Order Second Moment(FOSM) method to propagate input uncertainty through the infinite slope model,further developing the model and implementing it in the PISA-m software package(Haneberg,2007).Here we present an ArcPy implementation of PISA-m algorithms,which can be run from ESRI ArcMap in an entirely consistent georeferenced framework,and which we call "GIS Tool for Infinite Slope Stability Analysis"(GIS-TISSA).Users can select between different input options,e.g.,following a similar input style as for PISA-m,i.e., using an *** parameters input file,or providing each input parameter as a raster or constant value,through the program graphic user *** outputs can include FS mean and standard deviation estimates,the probability of failure(FS d a reliability index(RI) calculation for *** the same seismic analysis approach as in PISA-m, the Newmark acceleration can also be done,for which raster files of the mean,standard deviation,probability of exceedance,and RI are also *** of the code is done by replicating the results obtained with the PISA-m code for different input options,within a 10-5 relative *** Carlo modeling is also applied to validate GIS-TISSA outputs,showing a good overall correspondence.A case study was performed for Kannur district,Kerala,India,where an extensive landslide databa se for the year 2018 was available.81.19% of the actual

关键词： Landslides GIS-TISSA PISA-m FOSM Factor of Safety

A comparison of approaches in fitting continuum SEds

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Research in Astronomy and Astrophysics 2013年第4期13卷 420-434页

作者： Yao Liu david Madlener Sebastian wolf Hong-Chi Wang Purple Mountain Observatory and Key Laboratory for Radio Astronomy Chinese Academy of Sciences Graduate University of Chinese Academy of Sciences Institut fr Theoretische Physik und Astrophysik Universitt zu Kiel

We present a detailed comparison of two approaches, the use of a precalculated database and simulated annealing （SA）, for fitting the continuum spectral energy distribution （SEd） of astrophysical objects whose appearance is dominated by surrounding dust. While pre-calculated databases are commonly used to model SEd data, only a few studies to date employed SA due to its unclear accuracy and convergence time for this specific problem. From a methodological point of view, different approaches lead to different fitting quality, demand on computational resources and calculation time. We compare the fitting quality and computational costs of these two approaches for the task of SEd fitting to provide a guide to the practitioner to find a compromise between desired accuracy and available resources. To reduce uncertainties inherent to real datasets, we introduce a reference model resembling a typical circumstellar system with 10 free parameters. We derive the SEd of the reference model with our code MC3 d at 78 logarithmically distributed wavelengths in the range [0.3 μm, 1.3 mini and use this setup to simulate SEds for the database and SA. Our result directly demonstrates the applicability of SA in the field of SEd modeling, since the algorithm regularly finds better solutions to the optimization problem than a precalculated database. As both methods have advantages and shortcomings, a hybrid approach is preferable. While the database provides an approximate fit and overall probability distributions for all parameters deduced using Bayesian analysis, SA can be used to improve upon the results returned by the model grid.

关键词： methods numerical - radiative transfer - protoplanetary disks

Regular nicotine intake increased tooth movement velocity,osteoclastogenesis and orthodontically induced dental root resorptions in a rat model

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International Journal of Oral Science 2017年第3期9卷 174-184页

作者： christian kirschneck michael maurer michael wolf claudia reicheneder peter proff department of orthodontics university medical centre of regensburgRegensburgGermany department of oral and maxillofacial surgery university medical centre of regensburgRegensburgGermany department of orthodontics rheinische friedrich wilhelm university of bonnBonnGermany

Orthodontic forces have been reported to significantly increase nicotine-induced periodontal bone loss. At present, however, it is unknown, which further （side） effects can be expected during orthodontic treatment at a nicotine exposure corresponding to that of an average European smoker. 63 male Fischer344 rats were randomized in three consecutive experiments of 21 animals each （A/B/C） to 3 experimental groups （7 rats, 112/3）. （A） cone-beam-computed tomography （CBCT）; （B） histology/serology; （C） reverse- transcription quantitative real-time polymerase chain reaction （RT-qPCR）/cotinine serology--（1） control; （2） orthodontic tooth movement （OTM） of the first and second upper left molar （NiTi closed coil spring, 0.25 N）; （3） OTM with 1.89 mg-kg- 1 per day s.c. of L（- ）-nicotine. After 14 days of OTM, serum cotinine and IL-6 concentration as well as orthodontically induced inflammatory root resorption （OIIRR）, osteoclast activity （histology）, orthodontic tooth movement velocity （CBCT, within 14 and 28 days of OTM） and relative gene expression of known inflammatory and osteoclast markers were quantified in the dental-periodontal tissue （RT-qPCR）. Animals exposed to nicotine showed significantly heightened serum cotinine and IL-6 levels corresponding to those of regular European smokers. Both the extent of root resorption, osteoclast activity, orthodontic tooth movement and gene expression of inflammatory and osteoclast markers were significantly increased compared to controls with and without OTM under the influence of nicotine. We conclude that apart from increased periodontal bone loss, a progression of dental root resorption and accelerated orthodontic tooth movement are to be anticipated during orthodontic therapy, if nicotine consumption is present. Thus patients should be informed about these risks and the necessity of nicotine abstinence during treatment.

关键词： dental research inbred Fischer344 nicotine orthodontics rats root resorption tooth movement

Complications of newborn enterostomies

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World Journal of Clinical Cases 2018年第16期6卷 1101-1110页

作者： Lea wolf Stefan Gfroerer Henning Fiegel Udo Rolle department of Pediatric Surgery University Hospital Frankfurt Goethe-University Frankfurt am Main

AIM To evaluate the occurrence and severity of enterostomy complications in newborns suffering from different intestinal *** A 10-year retrospective cohort study(2008-2017) investigated newborns that underwent enterostomy formation and reversal for different intestinal disorders. Only infants less than 28 d old at the time of enterostomy creation were included in the study(corrected age was applied in the cases of preterm neonates). The patients were divided into two groups according to their underlying diseases. Group 1 included infants suffering from necrotizing enterocolitis(NEC), whereas Group 2 included newborns diagnosed with intestinal disorders other than NEC, such as meconium obstruction, anorectal malformation, focal intestinal perforation, ileus, intestinal atresia and volvulus. The primary outcome measure was enterostomy-related morbidity. The data were analyzed statistically using Pearson's χ2 test or Fisher's exact test for categorical variables and the Wilcoxon-Mann-Whitney U-Test for continuous variables. RESULTS In total, 76 infants met the inclusion criteria and were evaluated for enterostomy-related complications. Neither group showed significant differences regarding gender, gestational age, weight at birth or weight at enterostomy formation. Infants suffering from NEC(Group 1) were significantly older at enterostomy for-mation than the neonates of Group 2 [median(range), 11(2-75) d vs 4(1-101) d, P = 0.004)]. Significantly more ileostomies were created in Group 1 [47(92.2%) vs 16(64.0%), P = 0.007], whereas colostomies were performed significantly more often in Group 2 [2(3.9%) vs 8(32.0%), P = 0.002]. The initiation of enteral nutrition after enterostomy was significantly later in Group 1 infants than in Group 2 infants [median(range), 5(3-13) vs 3(1-9), P d 68.0% in Group 2(P =

关键词： Enterostomy closure Enterostomy Stoma Necrotizing enterocolitis Neonates Complications Enterostomy formation

Kaede for detection of Protein Oligomerization

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Molecular Plant 2013年第5期6卷 1453-1462页

作者： Heike wolf B. George Barisas KarI-Josef dietz Thorsten Seidel dynamic Cell Imaging Faculty of Biology Bielefeld University D-33501 Bielefeld Germany department of Chemistry Colorado State University Fort Collins CO 80523 usa Biochemistry and Physiology of Plants Faculty of Biology Bielefeld University D-33501 Bielefeld Germany

Photoconvertible fluorescent proteins such as Kaede are routinely used for tracking proteins, organelles, and whole cells. Kaede was the first identified photoconvertible fluorescent protein and has since become the most commonly used photoconvertible fluorescent protein in vertebrates. Kaede can be irreversibly converted from a green to a red fluo- rescent form upon UV/blue light irradiation and fluorescence of each form can be isolated separately by appropriate filter sets. Spectral properties of the Kaede forms allow F6rster resonance energy transfer （FRET） from the green form as donor to the red form as acceptor. As a sample containing oligomerized Kaede-containing proteins is exposed to UV or blue light, FRET first increases as green Kaede is converted to red and then decreases as the green donor becomes depleted. Thus, FRET information is potentially obtained from a number of independent measurements taken as photoconversion proceeds. We demonstrate here the application of this approach to detect homo-aggregation and conformational dynamics of plant pro- tein constructs. Structural alterations of 2-cys peroxiredoxin-Kaede were successfully detected depending on the redox state in living plant cells. Photoconversion was performed gradually and donor emission, acceptor emission, and FRET-derived sensitized acceptor emission were measured at each step of conversion. Since photoconvertible proteins have not been rou- tinely used in plants, two plasmids have been designed to facilitate plant applications. The plasmids allow either transient expression of Kaede-containing protein constructs in plant cells or Gateway cloning and stable transformation of plants.

关键词： protein structure fluorescence imaging protein-protein interaction.

Estimate of oxygen consumption and intracellular zinc concentration of human spermatozoa in relation to motility

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Asian Journal of Andrology 2003年第1期5卷 3-8页

作者： Ralf R.Henkel Kerstin defosse Hans-Wilhelm Koyro Norbert Weissmann wolf-Bernhard Schill Center for dermatology and Andrology University D-35385 Giessen GermanyCenter for Dermatology and Andrology University D-35385 Giessen GermanyInstitute for Plant Ecology University D-35385 Giessen GermanyCenter for Internal Medicine Justus Liebig University D-35385 Giessen GermanyCenter for Dermatology and Andrology University D-35385 Giessen Germany

Aim: To investigate the human sperm oxygen/energy consumption and zinc content in relation to motility. Methods: In washed spermatozoa from 67 ejaculates, the oxygen consumption was determined. Following calculation of the total oxygen consumed by the Ideal Gas Law, the energy consumption of spermatozoa was calculated. In addition, the zinc content of the sperm was determined using an atomic absorption spectrometer. The resulting data were correlated to the vitality and motility. Results: The oxygen consumption averaged 0.24μmol/106 sperm×24 h, 0.28μmol/106 live sperm×24 h and 0.85μmol/106 live & motile sperm×24 h. Further calculations revealed that sperm motility was the most energy consuming process (164.31 mJ/106 motile spermatozoa×24 h), while the oxygen consumption of the total spermatozoa was 46.06 mJ/106 spermatozoa×24 h. The correlation of the oxygen/ energy consumption and zinc content with motility showed significant negative correlations (r= -0.759; Pd r=-0.441; Pd. Conclusion: Poorly motile sperm are actually wasting the available energy. Moreover, our data clearly support the 'Geometric Clutch Model' of the axoneme function and demonstrate the importance of the outer dense fibers for the generation of sperm motility, especially progressive motility.

关键词： oxygen energy consumption human spermatozoa sperm motility zinc