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Sequence analysis of VP4 genes of wild type and culture adapted human rotavirus G1P[8]strains

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Asian Pacific Journal of Tropical Medicine 2011年第7期4卷 541-546页

作者： ritu Arora Ganesh S dhale Pooja r Patil shobha d Chitambar Enteric Viruses Group National Institute of VirologyPune India

Objective:To conduct a comparative analysis of the VP4 gene sequences of Indian wild type (06361,0613158,061060 and 0715880) and cell culture adapted(06361-CA,0613158-CA.061060- CA and 0715880-CA) G1P[8]rotavirus ***:Full-length VP4 genes of each of the four wild type G1P[8]rotavirus strains and their cell culture adapted counterparts displaying consistent cytopathic effect were subjected to rT-PCr amplification and nucleotide sequencing. results:All four cell culture adapted G1P[8]rotavirus strains showed nucleotide and amino acid substitutions in the VP4 gene as compared to their wild type *** number of substitutions however,varied from 1-64 and 1-13 *** substitutions were distributed in both VP5* and VP8* subunits of VP4 gene respectively of permeabilizalion and hemagglutinaling activity. The presence of unique amino acid substitutions was identified in two of the four wild type(V377G. S387N in 061060 and 1644L in 0715880) and all four cell culture adapted(A46V in 0613158-CA. T60r in 06361-CA,L237V.G389V and Q480H in 061060-CA and S615G and T625P in 0715880-CA) strains for the first time in the VP4 gene of P[8]*** acid substitutions generated increase in the hydrophilicity in the cell culture adapted rotavirus strains as compared to their corresponding wild type ***:Amino acid substitutions detected in the VP4 genes of G1P[8]rotavirus strains from this study together with those from other studies highlight occurrence of only strain and/or host specific substitutions during cell culture adaptation. Further evaluation of such substitutions for their role in attenuation,immunogenicity and conformation is needed for the development of newer rolavirus vaccines.

关键词： rotavirus Cell culture G1P VP4 gene

Molecular characterization of VP4,VP6,VP7 and NSP4 genes of group B rotavirus strains from outbreaks of gastroenteritis

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Asian Pacific Journal of Tropical Medicine 2011年第11期4卷 846-849页

作者： Anismrita Lahon shobha d Chitambar Enteric Viruses Group National Institute of VirologyPune-411001India

Objective:To characterize VP4,VP6,VP7 and NSP4 genes of representative GBr strains(NIV- ***-04622 and NIV-094456) delected as the major eliolngic agenl in the outbreaks of gastroenteritis in western ***:Fecal specimens collected during the outbreaks of gastroenteritis were processed for rNA ***-PCr using GBr ***7 and NSP4 gene specific primers,nucleotide sequencing of the amplicons and phylogenetic analysis of the ***:Phylogenetic analysis of all of the ***7 and NSP4 gene sequences revealed clustering of GBr strains in Indian-Bangladeshi lineage of genotype G2 with 95.8%- 99.4%nucleotide and 97.3%-100.0%amino acid ***,all three strains showed the presence of unique amino acid substitutions in the VP4 protein suggesting alteration in the antigenicity of outbreak strains of *** VP8* and VP5* regions of VP4 proteins showed respectively 0.5%-6.3%and 0.2%-1.1%amino acid divergence from human GBr strains of Indian-Bangladeshi ***:These data confirm the reported variability of VP8* region and suggest the possible role of this region in the perpetuation of GBr infections in the *** is the first study to document the phylogenetic relationship of VP4,***7 and NSP4 genes of GBr strains detected in the outbreaks of gastroenteritis from India with the CBr strains from other parts of world.

关键词： Acute gastroenteritis Group B rotavirus VP4 VP6 VP7 NSP4

An efficient Biginelli one-pot synthesis of new benzoxazole-substituted dihydropyrimidinones and thiones catalysed by trifluoro acetic acid under solvent-free conditions

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Chinese Chemical Letters 2009年第9期20卷 1059-1061页

作者： d.shobha M.Adharvana Chari K.H.Ahn department of Applied Chemistry Kyung Hee UniversitySuwonYongin-siSouth Korea

An efficient synthesis of benzoxazole-substituted 3,4-dihydropyrimidinones （dHPMs） using trifluoro acetic acid as the catalyst for the first time from an aldehyde, 13-keto ester and benzoxazole-substituted urea/thiourea under solvent-free conditions is described. Compared to the classical Biginelli reaction conditions, this new method consistently has the advantage of excellent yields （80-91%） and short reaction time （40-130 min） at reflux temperature. 2009 M. Adharvana Chad. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.

关键词： dihydropyrimidinones Trifluoro acetic acid Benzoxazole-substituted urea and thiourea

Efficiency of Severe recorded rainstorms over Maharashtra State

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Advances in Atmospheric Sciences 1995年第1期12卷 103-108页

作者： B.d.Kulkarni Miss shobha Nandargi O. N. dhar Indian Institute of Tropical Meteorology Pune -411 008 India Indian Institute of Tropical Meteorology Pune -411 008 India Indian Institute of Tropical Meteorology Pune -411 008 India

12 severe rainstorms have been experienced by different meteorological sub-divisions of Maharashtra State during the last 100-year period from 1891-1990. For each of the rainstorms efficiency factors (i.e. P/ M ratios) were worked out for maximum one-day duration and for three standard areas of 1000, 5000, and 10,000 km2. Comparison of these ratios with the past has shown that the most efficient rainstorm over Maharashtra was the rainstorm of June, 1908 over the Vidarbha region whose full dAd data as well as isohyetal pattern have been given.

关键词： rainstonn depth-area -duration (dAd) Efficiency factor of rainstorms Probable Maximum Precipitation (PMP)

Graphene-Based Coronal Hybrids for Enhanced Energy Storage

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Energy Material Advances 2021年第1期2021卷 218-232页

作者： Karthik Kiran Sarigamala shobha Shukla Alexander Struck Sumit Saxena Centre for research in Nanotechnology and Science Indian Institute of Technology Bombay400076MumbaiIndia Nanostructures Engineering and Modelling Laboratory Department of Metallurgical Engineering and Materials ScienceIndian Institute of Technology Bombay400076MumbaiIndia Faculty of Technology and Bionics Rhein-Waal University of Applied SciencesKleveGermany 47533

Functional materials with designer morphologies are anticipated to be the next generation materials for energy storage *** this manuscript,we have developed a holistic approach to enhance the surface area and hence the properties of nanostructures by synthesizing coronal nanohybrids of *** nanohybrids provide distinctive advantages in terms of performance and stability over vertically stacked nanocomposites reported in *** double hydroxide materials self-assembled as coronal lamellae on graphene shells have been synthesized and systematically *** coronal nanohybrids result in about a threefold increase in energy storage capacity as compared to their traditionally synthesized nanocomposite *** 3d graphene-based nanofibrils in the synthesized coronal nanohybrids provide mechanical support and connect the nodes of the double hydroxide lattices to inhibit *** morphologies such as coronal nanostructures increase the interaction surface of the nanostructure *** an approach is also expected to bring a paradigm shift in development of functional materials for various applications such as sensors,energy storage,and catalysis.

关键词： hydroxide hence shells

diagnostic performance of C-reactive protein level and its role as a potential biomarker of severe dengue in adults

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Asian Pacific Journal of Tropical Medicine 2020年第8期13卷 358-365页

作者： raghavendra rao Snehal Nayak Akhilesh K Pandey shobha U Kamath department of Medicine Kasturba Medical CollegeManipalIndia Manipal Academy of Higher Education ManipalIndia department of Biochemistry Kasturba Medical CollegeManipalIndia department of Community Medicine Kasturba Medical CollegeManipalIndia

Objective: To determine the relationship between C-reactive protein(CrP) level and the severity of dengue and the potential use of CrP in predicting acute dengue ***: A prospective observational study was performed on dengue patients admitted to a tertiary care hospital in southern India. All patients of age above 18 years, diagnosed with dengue were included in the study. The detailed laboratory parameters pertaining to dengue were recorded. CrP levels were estimated and compared between groups i.e. severe and non-severe dengue. CrP cut-off value was detected using the receiver-operator ***: Totally 98 patients with a mean age of 40 years were included. Among them, 11.2% of the patients suffered from severe dengue, 54.1% of the cases had non-severe dengue without warning signs, and 34.7% had non-severe dengue with warning signs. The median CrP was significantly higher in patients with severe dengue compared to patients with non-severe dengue(96.2 mg/d L vs. 5.3 mg/d L). Univariate logistic regression analysis showed that the odd’s ratio(Or) of CrP was 1.053(P≤0.001, 95% CI=1.029-1.078). CrP at a cutoff value of 21.6 mg/L(0.929 AUC) had excellent sensitivity(100%) and specificity(81.6%) in predicting severe dengue infection. Multivariate logistic regression analysis showed that CrP(Or=1.089, P=0.013) and ALT(Or=1.010, P=0.034) were statistically significant independent predictors of dengue ***: CrP level could be used as a potential biomarker to predict severity of dengue in adults.

关键词： Vector-borne Arbovirus Inflammation Biochemical Tropical disease

Evaluation of Hyperimmune Hen Egg Yolk derived Anti-Human rotavirus Antibodies (Anti-HrVIgY) against rotavirus Infection

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World Journal of Vaccines 2012年第2期2卷 73-84页

作者： Manika Buragohain Ganesh S. dhale Govind r. Ghalsasi shobha d. Chitambar Enteric Viruses department National Institute of Virology Pune India SPF Eggs division Venky’s (India) Ltd. Pune India

Oral delivery of specific IgY has been reported to be beneficial against rotavirus infection. However, the production of IgYs against globally prevalent human rotavirus (HrV) serotypes and their evaluation detailing the influence on the virological/histopathological consequences have not been reported to date. In the present study, anti-HrVIgY was generated in the eggs of specific pathogen free hens immunized with HrV serotypes G1 - G4 and G9 independently. Purified anti-HrVIgY preparations were tested to determine the ELISA and neutralizing antibody titers respectively in an indirect ELISA and cell culture based neutralization assay. Efficacy of pre and post infection treatment of anti-HrV-3IgY was assessed in an infant BALB/c mouse model of human rotavirus infection by monitoring percent diarrhea, severity and duration of diarrhea, intestinal viral load and histopathology. High (1:64000 - 1:512000) titered anti-HrVIgYs were obtained from the egg yolk of immunized hens with peak titer value (1:256000/1:512000) at 40 - 60 day of immunization. In-vitro, each of the anti-HrVIgY preparations showed the presence of multiserotypic neutralizing activity with high (1:1600 - ≥1:6400) homologous and low (≤1:50 - 1:800) heterologous titers. However, anti-HrV-3IgY neutralized all of the serotypes tested in the study indicating broader in-vitro neutralizing activity. In mice, post exposure treatment with anti-HrV-3IgY significantly reduced the extent of diarrhea and intestinal virus load and inhibited histopathological changes whereas pre exposure anti-HrV-3IgY treatment imparted immediate protection from development of rotavirus gastroenteritis. Thus, the anti-HrVIgY administered orally decreased morbidity and disease incidences in mice suggesting its potential implication in prophylactic and therapeutic usage in human to achieve reduction in rotavirus disease burden.

关键词： IgY rotavirus Mouse Model Oral Immunotherapy

rationally engineered 3d-dendritic cell-like morphologies of LdH nanostructures using graphene-based core-shell structures

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Microsystems & Nanoengineering 2019年第1期5卷 15-23页

作者： Karthik Kiran Sarigamala shobha Shukla Alexander Struck Sumit Saxena Centre for research in Nanotechnology and Science Indian Institute of Technology BombayMumbaiMH 400076India Nanostructures Engineering and Modeling Laboratory Department of Metallurgical Engineering and Materials ScienceIndian Institute of Technology BombayMumbaiMH 400076India Faculty of Technology and Bionics Rhein-Waal University of Applied Sciences47533 KleveGermany

Functionalization of graphene-based materials using chemical moieties not only modify the electronic structure of the underlying graphene but also enable in limited enhancement of targeted *** modification of graphene-based materials using other nanostructures enhances the effective properties by minimally modifying the properties of pristine graphene *** this pursuit,we have synthesized bio-inspired hierarchical nanostructures based on Ni–Co layered double hydroxide on reduced graphene oxide core–shells using template based wet chemical *** material synthesized have been characterized structurally and *** fabricated dendritic morphology of the composite delivers a high specific capacity of 1056 Cg^(−1).A cost effective solid state hybrid supercapacitor device was also fabricated using the synthesized electrode material which shows excellent performance with high energy density and fast charging capability.

关键词： backbone template shells

CHEMICAL COMPOSITION ANd ALLELOPATHIC ACTIVITY OF VOLATILE OIL OF TAGETES PALTULA L

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CHEMICAL COMPOSITION AND ALLELOPATHIC ACTIVITY OF VOLATILE O...