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In vivo femtosecond laser nanosurgery of the cell wall enabling patch-clamp measurements on filamentous fungi

Microsystems & Nanoengineering 2024年第2期10卷 251-267页

作者： Tanja Pajić katarina Stevanović Nataša v.Todorović Aleksandar J.krmpot Miroslav Živić Svetlana Savić-Šević Steva M.Lević Marina Stanić dejan Pantelić Brana Jelenković Mihailo d.Rabasović Institute of Physiology and Biochemistry“Ivan djaja” Faculty of BiologyUniversity of BelgradeStudentski trg 1611158BelgradeSerbia Institute for Biological Research“Siniša Stanković” University of BelgradeNational Institute of the Republic of SerbiaBulevar Despota Stefana 14211000BelgradeSerbia Institute of Physics Belgrade University of BelgradeNational Institute of the Republic of SerbiaPregrevica 11811080BelgradeSerbia University of Belgrade Faculty of AgricultureNemanjina Street 611080BelgradeSerbia Institute for Multidisciplinary Research University of BelgradeKneza Višeslava 111030BelgradeSerbia

Studying the membrane physiology of filamentous fungi is key to understanding their interactions with the environment and crucial for developing new therapeutic strategies for disease-causing ***,their plasma membrane has been inaccessible for a micron-sized patch-clamp pipette for pA current recordings due to the rigid chitinous cell ***,we report the first femtosecond IR laser nanosurgery of the cell wall of the filamentous fungi,which enabled patch-clamp measurements on protoplasts released from hyphae.A reproducible and highly precise(diffraction-limited,submicron resolution)method for obtaining viable released protoplasts was *** release from the nanosurgery-generated incisions in the cell wall was achieved from different regions of the *** plasma membrane of the obtained protoplasts formed tight and high-resistance(GΩ)contacts with the recording *** entire nanosurgical procedure followed by the patch-clamp technique could be completed in less than 1 *** to previous studies using heterologously expressed channels,this technique provides the opportunity to identify new ionic currents and to study the properties of the ion channels in the protoplasts of filamentous fungi in their native environment.

关键词： patch recording laser

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Extracellular vesicles-coupled miRNAs from oviduct and uterus modulate signaling pathways related to lipid metabolism and bovine early embryo development

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Journal of Animal Science and Biotechnology 2024年第4期15卷 1528-1549页

作者： Rosane Mazzarella karina Canon‑Beltran Yulia N.Cajas Meriem Hamdi Encina M.Gonzalez Juliano C.da Silveira Claudia L.v.Leal d.Rizos department of Animal Reproduction INIA-CSICMadridSpain department of Biochemistry and Molecular Biology Veterinary FacultyComplutense University of Madrid(UCM)MadridSpain department Agrarian Production Technical University of MadridUPMMadridSpain departamento de Ciencias Biologicas Universidad Tecnica Particular de LojaUTPLLojaEcuador department of Anatomy and Embryology FV-UCMMadridSpain department of veterinary Medicine FZEA-USPPirassunungaBrazil

Background Extracellular vesicles(Evs) present in oviductal(OF) and uterine fluid(UF) have been shown to enhance bovine embryo quality during in vitro culture by reducing lipid contents and modulating lipid metabolism-related genes(LMGs), while also influencing cell proliferation, suggesting their involvement on the regulation of different biological pathways. The regulation of signaling pathways related to cell differentiation, proliferation, and metabo-lism is crucial for early embryo development and can determine the success or failure of the pregnancy. Bioactive molecules within Evs in maternal reproductive fluids, such as micro RNAs(miRNAs), may contribute to this regulatory process as they modulate gene expression through post-transcriptional *** This study evaluated miRNA cargo in OF-Evs from the early luteal phase and UF-Evs from the mid-luteal phase, coinciding with embryo transit within oviduct and uterus in vivo, and its possible influence on LMGs and sign-aling pathways crucial for early embryo development. A total of 333 miRNAs were detected, with 11 exclusive to OF, 59 to UF, and 263 were common between both groups. From the 20 differentially expressed miRNAs, 19 up-regulated in UF-Evs(bta-miR-134, bta-miR-151-3p, bta-miR-155, bta-miR-188, bta-miR-181b, bta-miR-181d, bta-miR-224, bta-miR-23b-3p, bta-miR-24-3p, bta-miR-27a-3p, bta-miR-29a, bta-miR-324, bta-miR-326, bta-miR-345-3p, bta-miR-410, bta-miR-652, bta-miR-677, bta-miR-873 and bta-miR-708) and one(bta-miR-148b) in OF-Evs. These miRNAs were predicted to modulate several pathways such as Wnt, Hippo, MAPk, and lipid metabolism and degradation. differ-ences in miRNAs found in OF-Evs from the early luteal phase and UF-Evs from mid-luteal phase may reflect differ-ent environments to meet the changing needs of the embryo. Additionally, miRNAs may be involved, particularly in the uterus, in the regulation of embryo lipid metabolism, immune system, and *** Our study su

关键词： Embryo-maternal interaction Epigenetic Exosomes Mammalian Preimplantation Reproductive fluids

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A study on wildfire impacts on greenhouse gas emissions and regional air quality in South of Orléans,France

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Journal of Environmental Sciences 2024年第1期135卷 521-533页

作者： Chaoyang Xue Gisèle krysztofiak Yangang Ren Min Cai Patrick Mercier Frédéric Le Fur Corinne Robin Benoit Grosselin véronique daele Max RMcGillen Yujing Mu valéry Catoire Abdelwahid Mellouki Laboratoire de Physique et Chimie de l’Environnement et de l’Espace(LPC2E) CNRS–UniversitéOrléans–CNES(UMR 7328)Orléans Cedex 245071France Institut de Combustion AérothermiqueRéactivitéEnvironnement(ICARE)CNRSOrléans Cedex 245071France Research Centre for Eco-Environmental Sciences Chinese Academy of SciencesBeijing 100085China Lig’Air-Association de surveillance de la qualitéde l’air en région Centre-val de Loire Saint-Cyr-en-Val 45590France Mohammed v University RabatMorocco

Wildfire events are increasing globally which may be partly associated with climate change,resulting in significant adverse impacts on local,regional air quality and global *** September 2020,a small wildfire(burned area:36.3 ha)event occurred in Souesmes(Loiret-Cher,Sologne,France),and its plume spread out over 200 km on the following day as observed by the MOdIS *** on measurements at a suburban site(~50 km northwest of the fire location)in Orléans and backward trajectory analysis,young wildfire plumes were *** increases in gaseous pollutants(CO,CH_(4),N_(2)O,vOCs,etc.)and particles(including black carbon)were found within the wildfire plumes,leading to a reduced air *** factors,defined as EF(X)=ΔX/ΔCO(where,X represents the target species),of various trace gases and black carbon within the young wildfire plumes were determined accordingly and compared with previous *** in the ambient ions(such as ammonium,sulfate,nitrate,chloride,and nitrite in the particle-and gasphase)and aerosol properties(e.g.,aerosol water content,aerosol p H)were also quantified and ***,we estimated the total carbon and climate-related species(e.g.,CO_(2),CH_(4),N_(2)O,and BC)emissions and compared them with fire emission *** biomass burning emission inventories have uncertainties in estimating small fire burned areas and *** instance,we found that the Global Fire Assimilation System(GFAS)may underestimate emissions(e.g.,CO)of this small wildfire while other inventories(GFEd and FINN)showed significant *** that it is the first time to record wildfire plumes in this region,related atmospheric implications are presented and discussed.

关键词： Wildfire Air quality Emission factor Emission inventory vOLTAIRE supersite

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Energy-efficient joint UAv secure communication and 3d trajectory optimization assisted by reconfigurable intelligent surfaces in the presence of eavesdroppers

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defence Technology（防务技术） 2024年第1期31卷 537-543页

作者： Huang Hailong Mohsen Eskandari Andrey v.Savkin Wei Ni The department of Aeronautical and Aviation Engineering The Hong Kong Polytechnic UniversityHong KongChina School of Electrical Engineering and Telecommunications University of New South WalesSydney2052Australia data61 CSIROAustralia

We consider a scenario where an unmanned aerial vehicle(UAv),a typical unmanned aerial system(UAS),transmits confidential data to a moving ground target in the presence of multiple *** friendly reconfigurable intelligent surfaces(RISs) help to secure the UAv-target communication and improve the energy efficiency of the *** formulate an optimization problem to minimize the energy consumption of the UAv,subject to the mobility constraint of the UAv and that the achievable secrecy rate at the target is over a given *** present an online planning method following the framework of model predictive control(MPC) to jointly optimize the motion of the UAv and the configurations of the *** effectiveness of the proposed method is validated via computer simulations.

关键词： Unmanned aerial systems(UASs) Unmanned aerial vehicle(UAv) Communication security Eaves-dropping Reconfigurable intelligent surfaces(RIS) Autonomous navigation and placement Path planning Model predictive control

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Outcomes of long-acting injectable antipsychotics use in pregnancy:A literature review

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World Journal of Psychiatry 2024年第4期14卷 582-599页

作者： Ana v Pejčić Srdjan M Stefanović MilošN Milosavljević vladimir S Janjić Marko M Folić Nevena d Folić Jovana Z Milosavljević department of Pharmacology and Toxicology Faculty of Medical SciencesUniversity of KragujevacKragujevac 34000Serbia department of Pharmacy Faculty of Medical SciencesUniversity of KragujevacKragujevac 34000Serbia department of Clinical Pharmacology University Clinical Center KragujevacKragujevac 34000Serbia department of Psychiatry Faculty of Medical SciencesUniversity of KragujevacKragujevac 34000Serbia Clinic for Psychiatry University Clinical Center KragujevacKragujevac 34000Serbia Center for Pharmaceutical and Pharmacological Research Faculty of Medical SciencesUniversity of KragujevacKragujevac 34000Serbia department of Pediatrics Faculty of Medical SciencesUniversity of KragujevacKragujevac 34000Serbia Pediatric Clinic University Clinical Center KragujevacKragujevac 34000Serbia department of Anatomy Faculty of Medical SciencesUniversity of KragujevacKragujevac 34000Serbia

BACkGROUNd Women with a history of serious psychotic disorders are at increased risk of disease relapse during ***-acting injectable(LAI)antipsychotics have been widely used to improve adherence and prevent relapse in patients with various severe psychotic disorders,but there is a lack of high-quality data from previous research on the safety of LAI antipsychotics during *** To summarize relevant data on maternal,pregnancy,neonatal,and developmental outcomes from published cases of LAI antipsychotic use in *** A literature search was performed through November 11,2023,using three online databases:PubMed/MEdLINE,Scopus,and Web of *** reports or case series that reported information about the outcomes of pregnancy in women who used LAI antipsychotics at any point in pregnancy,with available full texts,were *** statistics,narrative summation,and tabulation of the extracted data were *** A total of 19 publications satisfied the inclusion criteria:3 case series,15 case reports,and 1 conference *** reported the outcomes of LAI antipsychotic use in 74 women and 77 *** use of secondgeneration LAI antipsychotics was reported in the majority(n=47;61.0%)of ***-generation LAI antipsychotics were administered during 30 pregnancies(39.0%).Most of the women(approximately 64%)had either satisfactory control of symptoms or no information about relapse,while approximately 12%of them had developed gestational diabetes mellitus.A minority of cases reported adverse outcomes such as stillbirth,spontaneous abortion,preterm birth,low birth weight,congenital anomalies,and neurological manifestations in ***,there were no reports of negative long-term developmental *** Currently available data seem reassuring,but further well-designed studies are required to properly evaluate the risks and benefits of LAI antipsychotic use during pregnancy.

关键词： Antipsychotic agents Long-acting injectable Pregnancy Outcome Review

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Exercise training improves long-term memory in obese mice

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Life Metabolism 2024年第1期3卷 37-50页

作者： Oliver k.Fuller Casey L.Egan Tina L.Robinson Nimna Perera Heidy k.Latchman Lauren v.Terry Emma d.McLennan Carolina Chavez Emma L.Burrows John W.Scott Robyn M.Murphy Henriette van Praag Martin Whitham Mark A.Febbraio Monash Institute of Pharmaceutical Sciences Monash UniversityMelbourneVictoria3052Australia La Trobe Institute for Molecular Science La Trobe UniversityMelbourneVictoria3086Australia Florey Institute of Neuroscience and Mental Health University of MelbourneMelbourneVictoria3052Australia department of Biomedical Sciences Charles E.Schmidt College of Medicineand Stiles-Nicholson Brain InstituteFlorida Atlantic UniversityJupiterFL 33458United States College of Life and Environmental Sciences University of BirminghamEdgbastonBirmingham B152TTUnited Kingdom

Obesity has been linked to a range of pathologies,including *** contrast,regular physical activity is associated with the prevention or reduced progression of ***,physical activity can improve memory and spatial cognition,reduce age-related cognitive decline,and preserve brain volume,but the mechanisms are not fully ***,we investigated whether any detrimental effects of high-fat diet(HFd)-induced obesity on cognition,motor behavior,adult hippocampal neurogenesis,and brain-derived neurotrophic factor(BdNF)could be mitigated by voluntary exercise training in male C57Bl/6 ***-induced impairment of motor function was not reversed by ***,voluntary wheel running improved long-term memory and increased hippocampal neurogenesis,suggesting that regular physical activity may prevent cognitive decline in obesity.

关键词： obesity exercise cognition

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Genetic factors of reproductive disorders in female

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Reproductive and developmental Medicine 2024年第3期8卷 186-195页

作者： Inna I.Mordovina Alina k.Chebanu Maksim Y.donnikov Tatiana A.Sinyukova Lyudmila v.kovalenko Larisa d.Belotserkovtseva department of Obstetrics Gynecologyand PerinatologyMedical Institute of Surgut State UniversitySurgutRussian Federation Scientific and Educational Center Medical Institute of Surgut State UniversitySurgutRussian Federation department of Pathophysiology and General Pathology Director of the Medical InstituteSurgut State UniversitySurgutRussian Federation Surgut Regional Clinical Center for Maternity and Childhood Protection SurgutRussian Federation

Successful reproduction in mammals requires gamete development,fertilization,and early embryonic *** in any of these processes can lead to infertility,recurrent miscarriages,and congenital *** clinical recognition of the genetic causes of female reproductive insufficiency using increasingly advanced genetic technologies poses a serious challenge for reproductive medicine in the 21st ***,the current literature on genetic factors involved in reproductive losses was summarized.A literature search was conducted using Web of Science,MEdLINE,and PubMed databases for articles written in English on the genetic causes of women’s reproductive health *** implementation of whole-exome and wholegenome sequencing is expected to identify numerous genetic factors responsible for oocyte *** will aid in increasing the likelihood of successful female reproductive function,improve the outcomes of assisted reproductive technologies,optimize treatment,and facilitate genetic diagnosis of patients.

关键词： Genetics Infertility Reproductive disorders Premature ovarian insufficiency

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HISP-1 Inhibits HSv-1 Infection in Cultured vero Cells

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Advances in Bioscience and Biotechnology 2024年第4期15卷 269-288页

作者： Sandra d. Adams kevin T. Bilyk Modukuri v. Ramani Gottumukkala v. Subbaraju department of Biology Montclair State University Montclair USA department of Chemistry Andhara University Visakhapatnam India

Herpes simplex virus-1 (HSv-1) remains a leading cause of viral disease worldwide and is spread by direct contact with infected lesions. There is no vaccine against HSv-1 infections and there remains a need to identify therapeutics that could reduce the spread. In this study various hispolon compounds were analyzed to determine their antiviral potential against HSv-1 infections in cultured vero cells. To determine the effects on infectivity and possible mechanisms of inhibition, the following assays were conducted. In vitro cytotoxicity assays were conducted to determine the effect of the compounds on cell viability and the maximum non-cytotoxic concentrations. Antiviral assays measured cell viability, percent inhibition of infection following treatment with the compounds, and the effect on the viral infection cycle. These effects were visualized using inverted light and fluorescent microscopy. Of the 24 hispolons tested, only hispolon pyrazole-1 (HISP-1) demonstrated antiviral effects. HISP-1 was demonstrated to effect early stages in HSv-1 infection in cultured vero cells (attachment, penetration, and post-penetration). In silico modeling analyses were conducted to analyze the interactions between HISP-1 and viral glycoprotein d (gd). HISP-1 is safe at concentrations tested and is effective in inhibiting infection of HSv-1 in cultured cells. HISP-1 has potential for therapeutic use as an antiviral against HSv-1 infection, could work in synergy with other antivirals that work be a different modality, and could be developed as a component of a topical agent to reduce the spread of HSv-1 infections.

关键词： Hispolon Herpes Simplex virus-1 Antiviral Curcumin

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Core needle biopsy for thyroid nodules assessment-a new horizon?

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World Journal of Clinical Oncology 2024年第5期15卷 580-586页

作者： david d dolidze Serghei Covantsev Grigorii M Chechenin Natalia v Pichugina Anastasia v Bedina Anna Bumbu department of Clinical Research and development Botkin HospitalMoscow 125284Russia department of Surgery Russian Medical Academy of Continuous Professional EducationMoscow 125445Russia Emergency Surgery No.76 Botkin HospitalMoscow 125284Russia department of Surgery Botkin HospitalMoscow 125284Russia department of Medical Ultrasonography Botkin HospitalMoscow 125284Russia Medicine Moscow State Medical University I.M.SechenovMoscow 119048Russia department of Oncology Botkin HospitalMoscow 125284Russia

Ultrasound-guided fine-needle aspiration is the standard for evaluating thyroid nodules with a high safety profile and a relatively low number of non-diagnostic cytological ***,this diagnostic method traditionally has its weak *** diagnostic categories such as BethesdaⅠ,ⅢandⅣare not reliable for thyroid carcinoma risk *** advancements in a core needle biopsy made it possible to use this tool as a new method for thyroid nodules *** main feature of this method is the use of thin needles(18-21G)and guns with an automatic trigger *** histological material collected with the use of a core needle biopsy is usually superior to ***,the core needle biopsy can be used as a complementary technique to a standard fine needle aspiration in difficult and dubious cases of thyroid neoplasia with uncertain malignant potential.

关键词： Core-needle biopsy Thyroid Follicular tumor Fine-needle aspiration Thyroid cancer

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Biomaterial engineering strategies for modeling the Bruch's membrane in age-related macular degeneration

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Neural Regeneration Research 2024年第12期19卷 2626-2636页

作者： Blanca Molins Andrea Rodríguez víctor Llorenç Alfredo Adán Group of Ocular Inflammation:Clinical and Experimental Studies Institut d’Investigacions Biomèdiques AgustíPi I Sunyer(IDIBAPS)BarcelonaSpain Institut Clínic d’Oftalmologia(ICOF) Hospital Clínic BarcelonaSpain

Age-related macular degeneration,a multifactorial inflammatory degenerative retinal disease,ranks as the leading cause of blindness in the ***,there is a scarcity of curative therapies,especially for the atrophic advanced form of age-related macular degeneration,likely due to the lack of models able to fully recapitulate the native structure of the outer blood retinal barrier,the prime to rget tissue of age-related macular *** in vitro systems rely on 2d monocultures unable to adequately reproduce the structure and function of the outer blood retinal barrier,integrated by the dynamic interaction of the retinal pigment epithelium,the Bruch's membrane,and the underlying *** Bruch's membrane provides structu ral and mechanical support and regulates the molecular trafficking in the outer blood retinal barrier,and therefo re adequate Bruch's membrane-mimics are key for the development of physiologically relevant models of the outer blood retinal barrie *** the last years,advances in the field of biomaterial engineering have provided novel approaches to mimic the Bruch's membrane from a variety of *** review provides a discussion of the integrated properties and function of outer blood retinal barrier components in healt hy and age-related macular degeneration status to understand the requirements to adequately fabricate Bruch's membrane biomimetic ***,we discuss novel materials and techniques to fabricate Bruch's membrane-like scaffolds for age-related macular degeneration in vitro modeling,discussing their advantages and challenges with a special focus on the potential of Bruch's membrane-like mimics based on decellularized tissue.

关键词： age-related macular degeneration Bruch's membrane decellularization retinal pigment epithelium scaffold

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