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Synthesis, Characterization and Remedial Action of Biogenic Silver Nanoparticles and Chitosan-Silver Nanoparticles against Bacterial Pathogens

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Journal of Renewable Materials 2022年第12期10卷 3093-3105页

作者： Piyush kumar Gupta d.karthik kumar M.Thaveena Soumya Pandit Somya Sinha R.Ranjithkumar Walaa F.Alsanie Vijay kumar Thakur department of Life Sciences School of Basic Sciences and ResearchSharda UniversityGreater Noida201310Uttar PradeshIndia Viyen Biotech LLP CoimbatoreTamil Nadu641031India department of Biotechnology Kongunadu Arts and Science CollegeCoimbatoreTamil Nadu641029India department of Biotechnology Graphic Era Deemed to be UniversityDehradunUttarakhand248002India department of Clinical Laboratories Sciences The Faculty of Applied Medical SciencesTaif UniversityTaif21944Saudi Arabia Biorefining and Advanced Materials Research Center SRUCEdinburghEH93JGUK department of Mechanical Engineering School of EngineeringShiv Nadar UniversityNoidaGreater Noida201314India School of Engineering University of Petroleum&Energy Studies(UPES)DehradunUttarakhand248007India

Custard apple is a dry land *** leaves exhibit different pharmacological *** the present study,both silver(Ag)nanoparticles and chitosan-coated Ag(Chi-Ag)nanoparticles were fabricated using the aqueous leaf extract of the custard apple *** preliminary phytochemical analysis,various types of phytocompounds were found in the aqueous leaf extract of the same ***,both nanoparticles were physiochemically *** analysis exhibited the fingerprint vibrational peaks of active bioactive compounds in plant extract,Ag nanoparticles,and Chi-Ag ***/Visible spectral analysis revealed the highest absorbance peak at 419 nm,indicating the presence of Ag *** analysis presented the face-centered cubic(FCC)structure of both prepared ***,the average crystalline size of both Ag nanoparticles and Chi-Ag nanoparticles was calculated to be 23 and 74 nm,*** analysis showed the spherical and cubical shapes of Ag nanoparticles and Chi-Ag nanoparticles,*** analysis indicated a peak around 3.29 keV,conforming to the binding energies of Ag *** biogenic nanomaterial also showed strong antibacterial activity against all tested bacterial pathogens.

关键词： Custard apple chitosan Ag nanoparticles Chi-Ag nanoparticles antibacterial

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Polyethylene glycol fusion repair of severed rat sciatic nerves reestablishes axonal continuity and reorganizes sensory terminal fields in the spinal cord

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Neural Regeneration Research 2025年第7期20卷 2095-2107页

作者： Emily A.Hibbard Liwen Zhou Cathy Z.Yang karthik Venkudusamy Yessenia Montoya Alexa Olivarez George d.Bittner dale R.Sengelaub Program in Neuroscience Indiana UniversityBloomingtonINUSA department of Neuroscience University of Texas at AustinAustinTXUSA

Peripheral nerve injuries result in the rapid degeneration of distal nerve segments and immediate loss of motor and sensory functions;behavioral recovery is typically *** used a plasmalemmal fusogen,polyethylene glycol(PEG),to immediately fuse closely apposed open ends of severed proximal and distal axons in rat sciatic *** have previously reported that sciatic nerve axons repaired by PEG-fusion do not undergo Wallerian degeneration,and PEG-fused animals exhibit rapid(within 2–6 weeks)and extensive locomotor ***,our previous report showed that PEG-fusion of severed sciatic motor axons was non-specific,i.e.,spinal motoneurons in PEG-fused animals were found to project to appropriate as well as inappropriate target *** this study,we examined the consequences of PEG-fusion for sensory axons of the sciatic *** adult male and female rats(Sprague–dawley)received either a unilateral single cut or ablation injury to the sciatic nerve and subsequent repair with or without(Negative Control)the application of *** action potentials recorded immediately after PEG-fusion repair confirmed conduction across the injury *** success of PEG-fusion was confirmed through Sciatic Functional Index testing with PEG-fused animals showing improvement in locomotor function beginning at 35 days *** 2–42 days postoperatively,we anterogradely labeled sensory afferents from the dorsal aspect of the hindpaw following bilateral intradermal injection of wheat germ agglutinin conjugated horseradish ***-fusion repair reestablished axonal *** to unoperated animals,labeled sensory afferents ipsilateral to the injury in PEG-fused animals were found in the appropriate area of the dorsal horn,as well as inappropriate mediolateral and rostrocaudal ***,despite having intact peripheral nerves,similar reorganizations of labeled sensory afferents were also observed contralateral to the injury and repa

关键词： axotomy dorsal horn peripheral nerve injury plasticity polyethylene glycol(PEG) sciatic nerve sensory terminals wheat germ agglutinin horseradish peroxidase

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Polyethylene glycol fusion repair of severed sciatic nerves accelerates recovery of nociceptive sensory perceptions in male and female rats of different strains

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Neural Regeneration Research 2025年第9期20卷 2667-2681页

作者： Liwen Zhou karthik Venkudusamy Emily A.Hibbard Yessenia Montoya Alexa Olivarez Cathy Z.Yang Adelaide Leung Varun Gokhale Guhan Periyasamy Zeal Pathak dale R.Sengelaub George d.Bittner department of Neuroscience University of TexasAustinTXUSA Program in Neuroscience Indiana UniversityBloomingtonINUSA department of Molecular Biosciences University of TexasAustinTXUSA

Successful polyethylene glycol fusion(PEG-fusion)of severed axons following peripheral nerve injuries for PEG-fused axons has been reported to:(1)rapidly restore electrophysiological continuity;(2)prevent distal Wallerian degeneration and maintain their myelin sheaths;(3)promote primarily motor,voluntary behavioral recoveries as assessed by the Sciatic Functional Index;and,(4)rapidly produce correct and incorrect connections in many possible combinations that produce rapid and extensive recovery of functional peripheral nervous system/central nervous system connections and reflex(e.g.,toe twitch)or voluntary *** preceding companion paper describes sensory terminal field reo rganization following PEG-fusion repair of sciatic nerve transections or ablations;howeve r,sensory behavioral recovery has not been explicitly explored following PEG-fusion *** the current study,we confirmed the success of PEG-fusion surgeries according to criteria(1-3)above and more extensively investigated whether PEG-fusion enhanced mechanical nociceptive recovery following sciatic transection in male and female outbred Sprague-dawley and inbred Lewis *** nociceptive responses were assessed by measuring withdrawal thresholds using von Frey filaments on the dorsal and midplantar regions of the *** von Frey filament tests were a more reliable method than plantar von Frey filament tests to assess mechanical nociceptive sensitivity following sciatic nerve *** withdrawal thresholds of the sciatic-mediated lateral dorsal region differed significantly across strain but not *** thresholds did not change significantly from baseline in chronic Unoperated and Sham-operated *** sciatic transection,all rats exhibited severe hyposensitivity to stimuli at the lateral dorsal region of the hindpaw ipsilateral to the ***,PEG-fused rats exhibited significantly earlier return to baseline withdrawal thresholds than Negative

关键词： autophagia axotomy collateral sprouting neuropathic pain peripheral nerve repair polyethylene glycol fusion(PEG-fusion) saphenous nerve sensory neurons sex and strain Wallerian degeneration

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Evaluating the effectiveness of marine actinobacterial extract and its mediated titanium dioxide nanoparticles in the degradation of azo dyes

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Journal of Environmental Sciences 2014年第4期26卷 775-782页

作者： S Priyaragini S Veena d Swetha L karthik G kumar K V Bhaskara Rao Molecular and Microbiology Research Laboratory Environmental Biotechnology Division School of Bio Sciences and Technology VIT University

Aim of the present study was to synthesize titanium dioxide nanoparticles （YiO2 NPs） from marine actinobacteria and to develop an eco-friendly azo-dye degradation method. A total of five actinobacterial isolates were isolated from Chennai marine sediments, Tamilnadu, India and analyzed for the synthesis of TiO2 NPs using titanium hydroxide. Among these, the isolate PSV 3 showed positive results for the synthesis of TiO2 NPs, which was confirmed by UV analysis. Further characterization of the synthesized TiO2 NPs was done using XRd, AFM and FI＇-IR analysis. Actinobacterial crude extract and synthesized TiO2 NPs was found efficient in degrading azo dye such as Acid Red 79 （AR-79） and Acid Red 80 （AR-80）. degradation percentage was found to be 81% for AR-79, 83% for AR-80 using actinobacterial crude extract and 84% for AR-79, 85% for AR-80 using TiO2 NPs. Immobilized actinobacterial ceils showed 88% for AR-79 and 81% for AR- 80, dye degrading capacity. degraded components were characterized by FT-IR and GC-MS analysis. The phytotoxicity test with 500 μg/mL of untreated dye showed remarkable phenotypic as well as cellular damage to Tagetes erecta plant. Comparatively no such damage was observed on plants by degraded dye components. In biotoxicity assay, treated dyes showed less toxic effect as compared to the untreated dyes.

关键词： actinobacteria azo dye degradation ZiO2 NPs biotoxicity Tagetes erecta

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A Study on the Protective Effect of Cynodon dactylon Leaves Extract in diabetic Rats

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Biomedical and Environmental Sciences 2011年第2期24卷 190-199页

作者： karthik d Ravikumar S department of Biotechnology PRIST University

Objective To investigate the antidiabetic, antioxidant and hypolipidemic efficacy of Cynodon dactylon in diabetic rats. Methods The experimental rats were randomly divided into three groups： Group I： control; Group II： Alloxan diabetic, untreated; and Group III： Alloxan diabetic treated with ethanolic extract of C. dactylon leaves （450 mg/kg bw）. Experimental diabetes was induced by alloxan in a single dose of 150 mg/kg bw. Results A Significant diminution of fasting blood sugar level was observed and also significant increase in HdL and decrease （P0.05） in cholesterol, triglyceride, LdL and VLdL were observed after 15 days of treatment. The investigation also revealed, the activities of AST, ALT, ALP, AP, LdH, and CPK （P0.05） were decreased in the extract‐supplemented group. The significant decrease in protein content and SOd, CAT, GPx, and GSH （P0.05） activity and increase in LPO in plasma were found to be ameliorated after treatment. Conclusion Our result supports the fact that administration of extract of C. dactylon leave is able to reduce hyperglycemia and hyperlipidemia risk and also reduced the oxidative stress in diabetic rats.

关键词： Cynodon dactylon Ethanolic extract GC‐MS Alloxan diabetes Antidiabetic Antioxidant Lipid profile Enzyme profile

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