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Study of Biofilm Formation and Antibiotic Resistance Pattern of Bacteria Isolated from Diabetic Foot Ulcers in Hôpital de Référence Saint Joseph, Kinshasa, Democratic Republic of Congo

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Advances in Microbiology 2021年第5期11卷 283-295页

作者： Jean-Marie Liesse Iyamba Victoire Marie Hermine Ngo Bassom cyprien mbundu lukukula Joseph Welo Unya Benjamin Kodondi Ngbandani Grégoire Mbusa Vihembo Nelson Nsiata Ngoma José Mulwahali Wambale Paul Tshilumbu Kantola N. B. Takaisi-Kikuni Laboratory of Experimental and Pharmaceutical Microbiology Faculty of Pharmaceutical Sciences University of Kinshasa Kinshasa Democratic Republic of Congo Centre Universitaire de Référence de Surveillance de la Résistance aux Antimicrobiens Faculty of Pharmaceutical Sciences University of Kinshasa Kinshasa Democratic Republic of Congo Service de Microbiologie H&ocirc pital de Référence Saint Joseph Limete-Kinshasa Democratic Republic of Congo

Foot infections resulting from biofilm producers and multi-drug resistant organisms is one of the most important complications of diabetes mellitus, as it can impede the wound healing process. This study was carried out in order to determine the antibiotic resistance pattern and the biofilm production in diabetic foot ulcers isolates. Clinical samples were collected from patients suffering from diabetic foot ulcers by using sterile swabs. Antibiotic susceptibility test was done using disk diffusion method on Mueller Hinton Agar. Biofilm formation was assessed by Crystal Violet Staining Method. Staphylococcus aureus isolates were resistant to ofloxacin (83.3%), ciprofloxacin (75.0%), trimethoprim-sulphamethoxazole (75.0%), and gentamicin (58.8%) but very sensitive to oxacillin (100.0%) and vancomycin (91.7%). Pseudomonas aeruginosa isolates showed resistance to the commonly used antibiotics such as ofloxacin, cefotaxime, ampicillin (81.8%), ceftazidime and imipenem (72.7%). The majority of bacteria studied were biofilm producers. This study showed that bacteria isolated from diabetic foot ulcers were biofilm producers and presented resistance to commonly used antibiotics. Knowledge on antibiotic sensitivity pattern and biofilm phenotype of the isolates will be helpful in determining the drugs for the treatment of diabetic ulcers.

关键词： Biofilm Formation Antibiotic Resistance Diabetic Foot Ulcers Democratic Republic of Congo

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Vaginal Colonization and Antibiotic Susceptibility Pattern of Group B <i>Streptococcus</i>Isolated from Pregnant Women in Maternitéde l’Hôpital Des Soeurs de Pauvres de Bergame de Kimbanseke, Kinshasa, Democratic Republic of Congo

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Advances in Microbiology 2021年第7期11卷 335-341页

作者： Jean-Marie Liesse Iyamba Pascal Murhula Mongane cyprien mbundu lukukula Benjamin Kodondi Ngbandani Junior Disashi Tshimpangila Grégoire Mbusa Vihembo Paul Tshilumbu Kantola José Mulwahaili Wambale Jacques Onokodi Kasongo N. B. Takaisi Kikuni Laboratory of Experimental and Pharmaceutical Microbiology Faculty of Pharmaceutical Sciences University of Kinshasa Kinshasa Democratic Republic of Congo Centre Universitaire de Référence de Surveillance de la Résistance aux Antimicrobien (CURS-RAM) Faculty of Pharmaceutical Sciences University of Kinshasa Kinshasa Democratic Republic of Congo

Group B Streptococcus (GBS) is a Gram-positive bacterium which often colonizes maternal vaginal and rectal epitheliums and can be transmitted to the neonate during delivery. GBS infections may cause significant maternal and neonatal morbidity, including sepsis, pneumonia and meningitis. In Democratic Republic of Congo, few studies have been done on GBS colonization of pregnant women. This study was conducted in Kinshasa, Democratic Republic of Congo in order to determine the prevalence of GBS vaginal colonization among pregnant women at a gestational age of 35 - 37 weeks and the antibiotic susceptibility. Vaginal swabs of 104 pregnant women were inoculated onto Chromatic Strepto B medium. GBS isolates were identified by Gram staining, catalase test, blue-green colonies and confirmed to be GBS by Strepto B latex test kit. Antibiotic susceptibility test was done using the disc diffusion method. The prevalence of GBS vaginal colonization was 23.07%. Of the isolates studied 100%, 75%, 62.5%, 50% were sensitive to vancomycin, clindamycin, cefazolin, and erythromycin respectively. Our findings seem to suggest that maternal GBS colonization rate in this study was higher compared to a previous report from Bukavu in Democratic Republic of Congo. All isolates were found to be sensitive to vancomycin which was the most effective antibiotic for the treatment of GBS infections.

关键词： Group B Streptococcus GBS Prevalence Antibiotic Susceptibility Democratic Republic of Congo

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Microscopic Features, Antioxidant and Antibacterial Capacities of Plants of the Congolese Cosmetopoeia, Raw Materials of Cosmeceuticals

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Journal of Biosciences and Medicines 2020年第9期8卷 149-166页

作者： Isaac Kaba Elaka Paulin Mutwale Kapepula Nadège Kabamba Ngombe Jonathan Mukanya Mpoyi Dorcas Mukundi Lukusa Messie Muipata Muabilwa Carmel Kandhe Gabha’Bey cyprien mbundu lukukula Boniface Nlandu Lukebakio Monique Kandolo Okuna Didi Mana Kialengila Paul Tshilumbu Kantola Centre d’Etudes des Substances Naturelles d’Origine Végétale (CESNOV) Faculty of Pharmaceutical Sciences University of Kinshasa Kinshasa Democratic Republic of Congo Laboratory of Experimental and Pharmaceutical Microbiology Faculty of Pharmaceutical Sciences University of Kinshasa Kinshasa Democratic Republic of Congo Institut National d’Etudes et de Recherches en Agronomie (INERA) University of Kinshasa Kinshasa Democratic Republic of Congo Bolimo SARL Kinshasa Democratic Republic of Congo Laboratory of Drug Analysis Faculty of Pharmaceutical Sciences University of Kinshasa Kinshasa Democratic Republic of Congo

Cosmeceuticals are the fastest growing products in the cosmetics field and represent a hybrid between cosmetics and drugs. The main benefits reported for cosmeceutical herbal extracts, used in skin care, include antioxidant and antimicrobial activities and the tyrosinase inhibiting effect. The aim of this study was to evaluate the antioxidant and antibacterial activities of extracts from Chromolaena odorata, Mitracarpus villosus, Senna alata, Tetradenia riparia, Cannabis sativa and Pterocarpus soyauxii used traditionally in the management of skin and skin annexes pathologies;additionally to determine their microscopical characteristics and their chromatographic fingerprints. Microscopic analyses showed the specific botanical microscopic characters for each plant that constitute the database useful for the identification and authentication of these botanicals. To the best of our knowledge, our work is the first to describe the microscopic histological characters of studied plants except for Cannabis sativa. The results of the TLC chromatographic analysis revealed the richness in phenolic compounds, terpenes in all extracts and Pterocarpus soyauxii contained coumarins. All extracts displayed high ABTS and DPPH radical-scavenging activities connected with their IC50 values at the concentration range of 1 - 125 μg/mL with Cannabis sativa and Pterocarpus soyauxii as the most active. All plant extracts exhibited antibacterial activity on Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus to varying degrees. Cannabis sativa exhibited strong effect on Staphylococcus aureus and Escherichia coli. Chromolaena odorata and Tetradenia riparia showed a marked effect on Escherichia coli. Although certain crude extracts from studied botanicals have been shown to be inactive, fractionation studies are needed to determine the active f

关键词： Cannabis sativa Chromolaena odorata Cosmeceuticals Cosmetopoeia Mitracarpus villosus Pterocarpus soyauxii Senna alata Tetradenia riparia Yelena

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