检索结果-南通市图书馆

Reactive oxygen species-scavenging nanoparticles coated with chondroitin sulfate protect cartilage against osteoarthritis in vivo

在线全文

学校读者我要写书评

暂无评论

Nano Research 2023年第2期16卷 2786-2797页

作者： Zhaoyi Wang Hao Xiong Zihe Zhai Yuejun Yao Tong Zhou Haolan Zhang cunyi fan Changyou Gao MOE Key Laboratory of Macromolecular Synthesis and Functionalization International Research Center for X PolymersDepartment of Polymer Science and EngineeringZhejiang UniversityHangzhou 310027China Department of Orthopedics Shanghai Jiao Tong University Affiliated Sixth People’s HospitalShanghai 200233China Center for Healthcare Materials Shaoxing InstituteZhejiang UniversityShaoxing 312035China Department of Orthopedics The Second Affiliated HospitalZhejiang University School of MedicineHangzhou 310009China Shanghai Engineering Research Center for Orthopaedic Material Innovation and Tissue Regeneration Building 3Langu Science and Technology ParkLane 70Haiji 6th RoadShanghai 201306China

Osteoarthritis(OA)is a prevalent chronic inflammatory disease in joints.Current interventions confront systemic toxicity and insufficient bioavailability.The unbalanced microenvironment of OA joints mainly fosters over-expressed reactive oxygen species(ROS),extracellular matrix disintegration,and apoptosis of chondrocytes.In this study,a kind of ROS-scavenging,biodegradable and drug-free nanoparticles(PP NPs)were constructed by the crosslinking of poly(propylene fumarate)(PPF)and ROS-scavenging poly(thioketal)(PTK).The high content of PTK and high crosslinking density of PPF and PTK innovatively endowed the NPs with slow degradation and prolonged ROS-elimination ability.The NPs were further surface-modified with chondroitin sulfate(CS),one of the dietary supplements for osteoarthritis.The intrinsic properties of resultant PP-CS NPs were excavated in vitro and in vivo.The PP-CS NPs could desirably consume 1,10-diphenyl-2-picrylhydrazyl(DPPH)radicals without toxicity to RAW264.7 cells in vitro.With an average diameter of~300 nm,the PP-CS NPs could be intra-articular administrated in OA rats and showed prolonged joint retention time,allowing only one injection per month.Moreover,the PP-CS NPs possessed a prolonged ROS depletion and M2 macrophage induction effect,down-regulated inflammatory cytokines,and reduced glycosaminoglycans loss.Consequently,the PP-CS NPs protected articular surface erosion,inhibited uneven cartilage matrix,and attenuated OA progression.

关键词： reactive oxygen species polythioketal osteoarthritis nanoparticles chondroitin sulfate

Non-electric bioelectrical analog strategy by a biophysical-driven nano-micro spatial anisotropic scaffold for regulating stem cell niche and tissue regeneration in a neuronal therapy

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bioactive Materials 2023年第2期20卷 319-338页

作者： Xiangyun Yao Lei Zhan Zhiwen Yan Juehong Li Lingchi Kong Xu Wang Huimin Xiao Huiquan Jiang Chen Huang Yuanming Ouyang Yun Qian cunyi fan Department of Orthopedics Shanghai Jiao Tong University Affiliated Sixth People’s HospitalShanghai200233China Shanghai Engineering Research Center for Orthopaedic Material Innovation and Tissue Regeneration Shanghai200233China Youth Science and Technology Innovation Studio of Shanghai Jiao Tong University School of Medicine Shanghai200233China Key Laboratory of Textile Science and Technology College of TextilesDonghua UniversityShanghai201620China College of Fisheries and Life Science Shanghai Ocean UniversityShanghai201306China

The slow regenerating rate and misdirected axonal growth are primary concerns that disturb the curative outcome of peripheral nerve repair.Biophysical intervention through nerve scaffolds can provide efficient,tunable and sustainable guidance for nerve regrowth.Herein,we fabricate the reduced graphene oxide(rGO)/polycaprolactone(PCL)scaffold characterized with anisotropic microfibers and oriented nanogrooves by electrospinning technique.Adipose-derived stem cells(ADSCs)are seeded on the scaffolds in vitro and the viability,neural differentiation efficiency and neurotrophic potential are investigated.RGO/PCL conduits reprogram the phenotype of seeded cells and efficiently repair 15 mm sciatic nerve defect in rats.In summary,biophysical cues on nerve scaffolds are key determinants to stem cell phenotype,and ADSC-seeded rGO/PCL oriented scaffolds are promising,controllable and sustainable approaches to enable peripheral nerve regeneration.

关键词： Reduced graphene oxide Nerve regeneration Biophysical Bioelectrical Nano-micro Adipose-derived stem cell

Dual-engineered cartilage-targeting extracellular vesicles derived from mesenchymal stem cells enhance osteoarthritis treatment via miR-223/NLRP3/pyroptosis axis:Toward a precision therapy

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bioactive Materials 2023年第12期30卷 169-183页

作者： Weixuan Liu Anqi Liu Xujun Li Ziyang Sun Zhenghua Sun Yaru Liu Gang Wang Dan Huang Hao Xiong Shiyang Yu Xintao Zhang cunyi fan Department of Sports Medicine Peking University Shenzhen HospitalShenzhen Peking University-The Hong Kong University of Science and Technology Medical CenterShenzhen518036China Shanghai Engineering Research Center for Orthopedic Material Innovation and Tissue Regeneration Shanghai201306China Department of Orthopedics Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai200233China Department of Orthodontics Shanghai Stomatological Hospital&School of StomatologyFudan UniversityShanghai200001China Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases Fudan UniversityShanghai200001China Minhang Hospital Fudan UniversityShanghai201199China

Osteoarthritis(OA)is the most common disabling joint disease with no effective disease modifying drugs.Extracellular vesicles released by several types of mesenchymal stem cells could promote cartilage repair and ameliorate OA pathology in animal models,representing a novel therapeutic strategy.In this study,we demonstrated that extracellular vesicles derived from human umbilical cord mesenchymal stem cells(hUC-EVs)could maintain chondrocyte homeostasis and alleviate OA,and further revealed a novel molecular mechanism of this therapeutic effect.miR-223,which could directly bind with the 3′UTR of NLRP3 mRNA,was found to be a key miRNA for hUC-EVs to exert beneficial effects on inflammation inhibiting and cartilage protecting.For enhancing the effect on mitigating osteoarthritis,exogenous miR-223 was loaded into hUC-EVs by electroporation,and a collagen II-targeting peptide(WYRGRL)was modified onto the surface of hUC-EVs by genetic engineering to achieve a more targeted and efficient RNA delivery to the cartilage.The dual-engineered EVs showed a maximal effect on inhibiting the NLRP3 inflammasome activation and chondrocyte pyroptosis,and offered excellent results for the treatment of OA.This study provides a novel theoretical basis and a promising therapeutic strategy for the application of engineered extracellular vesicles in OA treatment.

关键词： Osteoarthritis Extracellular vesicles NLRP3 inflammasome Pyroptosis Cartilage-targeting

Functional reconstruction following brachial plexus root avulsion

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2007年第1期2卷 54-57页

作者： Guixin Sun cunyi fan Yudong Gu Department of Trauma Medicine Shanghai East Hospital Affiliated to Shanghai Tongji University Shanghai 200120 China Department of Orthopaedics Sixth Hospital Shanghai Jiao Tong University Shanghai 200233 China Department of Hand Surgery Huashan Hospital Affiliated to Fudan University Shanghai 200040 China

OBJECTIVE： To sum up the treatment of brachial plexus root avulsion and the progress in functional reconstruction and rehabilitation following brachial plexus root avulsion. DATA SOURCES： A search of Medline was performed to select functional reconstruction and rehabilitation following brachial plexus injury-related English articles published between January 1990 and July 2006, with key words of ＂brachial plexus injury, reconstruction and rehabilitation＂. Meanwhile, a computer-based search of CBM was carried out to select the similar Chinese articles published between January 1998 and July 2006, with key words of ＂brachial plexus injury, reconstruction and rehabilitation＂. STUDY SELECTION： The materials were checked primarily, and the literatures of functional reconstruction and rehabilitation of brachial plexus injury were selected and the full texts were retrieved. Inclusive criteria： ①Functional reconstruction following brachial plexus injury. ②Rehabilitation method of brachial plexus injury. Exclusive criteria： Reviews, repetitive study, and Meta analytical papers. DATA EXTRACTION： Forty-six literatures about functional reconstruction following brachial plexus injury were collected, and 36 of them met the inclusive criteria. DATA SYNTHESIS： Brachial plexus injury causes the complete or incomplete palsy of muscle of upper extremity. The treatment of brachial plexus is to displace not very important nerves to the distal end of very important nerve, called nerve transfer, which is an important method to treat brachial plexus injury. Postoperative rehabilitations consist of sensory training and motor functional training. It is very important to keep the initiativeness of exercise. Besides recovering peripheral nerve continuity by operation, combined treatment and accelerating neural regeneration, active motors of cerebral cortex is also the important factor to reconstruct peripheral nerve function. CONCLUSION： Consciously and actively strengthening functional exercise after operation is helpful to form cerebral p

关键词： brachial plexus root avulsion functional reconstruction review literature

基于科技成果转化的概念验证中心研究综述与展望

在线全文

学校读者我要写书评

暂无评论

杭州科技 2024年第2期55卷 46-51页

作者：许彬彬周超范存义上海交通大学医学院附属第六人民医院上海200233

科技成果转化是建设科技强国的着力点,概念验证中心是科技成果转化的重要环节,得到学术界的高度关注和研究。概念验证中心的定义分为三类:新型组织模式、可行性验证平台和成果转化平台。通过对国内外研究的梳理,本文提出概念验证中心的... 详细信息

科技成果转化是建设科技强国的着力点,概念验证中心是科技成果转化的重要环节,得到学术界的高度关注和研究。概念验证中心的定义分为三类:新型组织模式、可行性验证平台和成果转化平台。通过对国内外研究的梳理,本文提出概念验证中心的功能,即技术评估、创新指导、融资助力和教育培训。概念验证中心发挥了加速科学创新到技术创新、助推科技成果转化和夯实科技创新基础的作用。概念验证中心对成果转化方案的技术、生产、投融资和商业可行性进行全面分析,并提供所需的资源和服务,促进方案的优化、迭代和转化。最后,提出进一步发展概念验证中心的建议,希望在政府引领、完善流程、人才培养和组织管理体系创新方面进行强化和突破。

关键词：概念验证中心评估评价科技创新科技成果转化创业指导

热处理条件对IrSn纳米颗粒结构及丙烷脱氢性能影响

在线全文

学校读者我要写书评

暂无评论

山西化工 2024年第6期44卷 1-2,7页

作者：范存毅马瑞梁永襄阳市公共检验检测中心湖北襄阳441058 化学与精细化工广东省实验室广东汕头515041

丙烷脱氢是低碳烯烃生产和页岩气高附加值应用的重要途径之一。通常负载型Pt-Sn双金属催化剂用于该过程面临催化剂失活快、再生流程繁琐的问题。Ir作为配位金属在均相脱氢反应中被证明具有很高的C-H键活化能力,但应用于多相催化的烷烃... 详细信息

丙烷脱氢是低碳烯烃生产和页岩气高附加值应用的重要途径之一。通常负载型Pt-Sn双金属催化剂用于该过程面临催化剂失活快、再生流程繁琐的问题。Ir作为配位金属在均相脱氢反应中被证明具有很高的C-H键活化能力,但应用于多相催化的烷烃脱氢反应未见报道。本文通过改变IrSn双金属催化剂的合成条件,探讨焙烧对Ir-Sn双金属纳米颗粒结构及催化丙烷脱氢性能影响。结果表明,焙烧不仅导致催化剂纳米颗粒严重烧结,而且降低双金属纳米颗粒合金化程度,极大影响催化性能。

关键词：焙烧合金结构元素分布丙烷脱氢

The influence of reduced graphene oxide on stem cells:a perspective in peripheral nerve regeneration

在线全文

学校读者我要写书评

暂无评论

Regenerative Biomaterials 2021年第4期8卷 88-96页

作者： Xiangyun Yao Zhiwen Yan Xu Wang Huiquan Jiang Yun Qian cunyi fan Department of Orthopedics Shanghai Jiao Tong University Affiliated Sixth People’s Hospital600 Yishan RoadShanghai 200233China Shanghai Engineering Research Center for Orthopaedic Material Innovation and Tissue Regeneration 600 Yishan RoadShanghai 200233China Youth Science and Technology Innovation Studio of Shanghai Jiao Tong University School of Medicine 600 Yishan RoadShanghai 200233China College of Fisheries and Life Science Shanghai Ocean University999 Metro loop Road ShanghaiChina

Graphene and its derivatives are fascinating materials for their extraordinary electrochemical and mechanical properties.In recent decades,many researchers explored their applications in tissue engineering and regenerative medicine.Reduced graphene oxide(rGO)possesses remarkable structural and functional resemblance to graphene,although some residual oxygen-containing groups and defects exist in the structure.Such structure holds great potential since the remnantoxygenated groups can further be functionalized or modified.Moreover,oxygen-containing groups can improve the dispersion of rGO in organic or aqueous media.Therefore,it is preferable to utilize rGO in the production of composite materials.The rGO composite scaffolds provide favorable extracellular microenvironment and affect the cellular behavior of cultured cells in the peripheral nerve regeneration.On the one hand,rGO impacts on Schwann cells and neurons which are major components of peripheral nerves.On the other hand,rGO-incorporated composite scaffolds promote the neurogenic differentiation of several stem cells,including embryonic stem cells,mesenchymal stem cells,adipose-derived stem cells and neural stem cells.This review will briefly introduce the production and major properties of rGO,and its potential in modulating the cellular behaviors of specific stem cells.Finally,we present its emerging roles in the production of composite scaffolds for nerve tissue engineering.

关键词： reduced graphene oxide nerve regeneration tissue engineering biomaterials stem cell

Establishment of an animal model of extra-vertebral foramen cervical nerve entrapment by local electrical stimulation and selection of suitable stimulation parameters

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2011年第29期6卷 2284-2289页

作者： Jinwu Wang Juliet Hou Qi Li Jian Tang Biyu Rui Shangchun Guo Haitao Jiang cunyi fan You Wang Zhenan Zhu Kerong Dai Guang H Yue Department of Orthopedic Surgery the Ninth People＇s Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200011 China Department of Physical Medicine ＆ Rehabilitation the Cleveland Clinic Cleveland Ohio 44195 USA Department of Orthopaedic Surgery the Sixth Municipal People＇s Hospital Shanghai Jiao Tong University Shanghai 200233 China Biomedical Engineering the Cleveland Clinic Cleveland Ohio 44195 USA

A rat model of extra-vertebral foramen cervical nerve entrapment was established according to the following parameters： stimulation intensity 20 V; frequency 50 Hz; pulse width 200 μs; duration 333 ms/s for a total of 8 hours. After the electrical stimulation, rats exhibited mild muscle fiber atrophy, mild inflammatory exudates, connective tissue local fibrosis and chondrocyte metaplasia. Mean muscle fiber cross-sectional area was reduced. The nerve myelin sheath continuity was partially demyelinated. The microstructure of nerve cells was disrupted and these symptoms worsened with prolongation of the stimulation. The shoulder, neck and upper extremity muscles on the tested side demonstrated positive sharp waves and fibrillations. The severity increased with continuation of the stimulation. High amplitude and polyphasic motor unit potentials gradually appeared. Similar findings were seen in the contralateral side, but at a less severe level.

关键词： chronic neck-shoulder pain electrical stimulation extra-vertebral foramen cervical nerve entrapment syndrome animal model

玛纳斯县土地制度改革对农用地流转的影响研究

在线全文

学校读者我要写书评

暂无评论

中国资源综合利用 2024年第2期42卷 36-38页

作者：马子凡王保力陈雪茹余晨蕾刘冰范存义新疆农业大学新生学院新疆农业大学公共管理学院乌鲁木齐830000

中国是个农业大国,土地是中国农村最重要的资源,土地问题解决的关键是对现有的土地制度和土地流转制度进行改革创新,构建与时俱进、因地制宜的土地流转模式和支撑体系。本文通过问卷调查法与深度访谈法对玛纳斯县土地制度改革对农用地... 详细信息

中国是个农业大国,土地是中国农村最重要的资源,土地问题解决的关键是对现有的土地制度和土地流转制度进行改革创新,构建与时俱进、因地制宜的土地流转模式和支撑体系。本文通过问卷调查法与深度访谈法对玛纳斯县土地制度改革对农用地流转的影响现状进行调查,然后采用描述性统计法和归纳分析法对玛纳斯县土地制度改革及土地流转情况进行分析,发现问题,提出对策建议。在土地流转过程中,玛纳斯县存在部分突出问题,即流转程序不规范,缺乏有效监督,土地流转信息时效差,缺乏流转交易平台,土地碎片化严重,整合困难。因此,要规范土地流转程序,实施有效监督,加强土地流转政策宣传,建立土地流转平台,优化空间布局,实现土地规模经营。

关键词：土地制度改革土地流转农用地影响因素玛纳斯县

维普期刊数据库博看期刊