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Structure-function relationship in viral RNA genomes: The case of hepatitis C virus

World Journal of Medical Genetics 2014年第2期4卷 6-18页

作者： cristina romero-López Alfredo Berzal-Herranz Instituto de Parasitología y Biomedicina López-Neyra IPBLN-CSICPTS GranadaArmilla 18016 Granada Spain

The acquisition of a storage information system beyond the nucleotide sequence has been a crucial issue for the propagation and dispersion of RNA viruses. This system is composed by highly conserved, complex structural units in the genomic RNA, termed functional RNA domains. These elements interact with other regions of the viral genome and/or proteins to direct viral translation, replication and encapsidation. The genomic RNA of the hepatitis C virus(HCV) is a good model for investigating about conserved structural units. It contains functional domains, defined by highly conserved structural RNA motifs, mostly located in the 5'-untranslatable regions(5'UTRs) and 3'UTR, but also occupying long stretches of the coding sequence. Viral translation initiation is mediated by an internal ribosome entry site located at the 5' terminus of the viral genome and regulated by distal functional RNA domains placed at the 3' end. Subsequent RNA replication strongly depends on the 3'UTR folding and is also influenced by the 5' end of the HCV RNA. Further increase in the genome copy number unleashes the formation of homodimers by direct interaction of two genomic RNA molecules, which are finally packed and released to the extracellular medium. All these processes, as well as transitions between them, are controlled by structural RNA elements that establish a complex, direct and long-distance RNARNA interaction network. This review summarizes current knowledge about functional RNA domains within the HCV RNA genome and provides an overview of the control exerted by direct, long-range RNA-RNA contacts for the execution of the viral cycle.

关键词： Functional RNA domain Cis-acting replicating element Hepatitis C virus Internal ribosome entry site RNA-RNA interaction Untranslatable region

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Assessment of the Quality and Shelf-Life in Enriched n3 PUFA Raw Beef Patties Using Dry Soybean Sprouts as Antioxidant

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Food and Nutrition Sciences 2014年第7期5卷 658-670页

作者： Mara cristina romero Oscar Alfredo Garro Ana María romero Ariel Germán Michaluk Mirtha Marina Doval María Alicia Judis Laboratorio de Industrias Alimentarias II Universidad Nacional del Chaco AustralPresidencia Roque Sáenz PenaArgentina CONICET Consejo Nacional de Investigaciones Científicas y TécnicasBuenos AiresArgentina

The appearance of fresh meat is a major determinant of its appeal to consumers. Ground beef in retail display oxidizes at a faster rate than whole muscle products;the use of antioxidants to break the radical chain reaction in the oxidation process is an effective method for slowing oxidation. Soybean is a complex matrix with several bioactive compounds, including peptides and proteins, isoflavones, saponins, and other compounds with antioxidant properties. This study was conducted for 1) to evaluate the antioxidant properties of dry soybeans sprouts (DSS) from Glycine max (L.) Merr., MUNASQA?;2) to evaluate quality characteristics of raw beef patties added with dried soybean sprouts at different levels (0.5%, 1% and 2%) and soybean oil as a replacer of fat;and 3) to estimate color changes and lipid oxidation of n3 fortified raw beef under frozen storage. Results showed that dry soybean sprouts had a high level of superoxide dismutase enzyme, high total phenolics and flavonoids content and appreciable vitamin c content. Although reduction power was low, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azino-bis(3-ethylbenzthiazoline-6-ulphonic acid) (ABTS) radicals scavenging activity were elevated. When different concentrations of DSS were incorporated to enriched beef patties with soybean oil, the nutritional value was slightly affected, while that n3 and n6 fatty acid content were increased around three twice. The beef patties formulated with 1% of natural antioxidant showed significantly (p < 0.05) better color stability than those without antioxidants. The lipid oxidation was inhibited in all cases, and was strongly effective at 1% concentration of DSS being the reduced percent of thiobarbituric acid reactive substances (TBARS) comparable to butilated hydroxianisole (BHA). The results shown in this research suggest that dry soybeans sprouts could also be used as a cheap natural antioxidant source for meat products.

关键词： Fresh Meat Products Natural Antioxidant Nutritional Composition Color Lipid Oxidation

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Perioperative management and early complications after intestinal resection with ileocolonic anastomosis in Crohn’s disease:analysis from the PRACTICROHN study

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Gastroenterology Report 2019年第3期7卷 168-175,I0001,I0002页

作者： Ana Gutierrez Montserrat Rivero Maria Dolores Martin-Arranz Valle Garcia Sanchez Manuel Castro Jesus Barrio Ruth de Francisco Manuel Barreiro-de Acosta Berta Julia Luis Cea-Calvo cristina romero Natalia Borruel Sainz Eugeni Domenech Gastroenterology Department General University Hospital of Alicante and Centro de Investigacion Biomedica en Red de Enfermedades Hepaticas y Digestivas(CIBERehd)CarlosⅢHealth InstituteMadridSpain Gastroenterology Department Marques de Valdecilla General University HospitalSantanderSpain Gastroenterology Department La Paz General University HospitalInstituto de Investigacion Biomedica La Paz(IdiPaz)MadridSpain Clinical Management of Gastrointestinal Tract Diseases Unit Reina Sofia University HospitalMaimonides Institute of Biomedical Research of Cordoba(IMIBIC)Cordoba UniversityCordobaSpain Gastroenterology Department Valme University HospitalSevillaSpain Gastroenterology Department University HospitalRio HortegaValladolidSpain Gastroenterology Department University Hospital Central de AsturiasOviedoSpain Gastroenterology Department Inflammatory Bowel Disease UnitUniversity Hospital Complex of Santiago de CompostelaSantiago de CompostelaSpain Medical Department Merck Sharp and DohmeMadridSpain Crohn-Colitis Care Unit(UACC) Digestive Tract ServiceHospital Universitari Vall d’HebronBarcelonaSpain Gastroenterology Department Germans Trias i Pujol University Hospital and CIBERehdBadalonaSpain

Background:This study is aimed at describing the prevalence of and risk factors associated with early post-operative complications after Crohn’s disease-related intestinal ***:This was a retrospective analysis of data from the PRACTICROHN *** Crohn’s disease patients who underwent ileocolonic resection with ileocolonic anastomosis between January 2007 and December 2010 were *** complications evaluated included death,ileus,anastomotic leak,abscess,wound infection,catheter-related infection,digestive bleeding and other extra-abdominal infections that occurred in the 30 days after ***:A total of 364 patients(median age at surgery 38 years and 50%men)were *** for surgery was:stricturing disease(46.4%),penetrating disease(31.3%),penetrating and stricturing disease(14.0%)or resistance to medical treatment(5.8%).Early complications were recorded in 100(27.5%)patients,with wound infection,intra-abdominal abscess and anastomotic leakage being the most frequent *** hospitalization duration was 16 days for patients with complications vs.9 days without complications(P<0.001).Complications were more common among patients with penetrating disease(36/114,31.6%)and those refractory to treatment(9/21,42.9%)compared with stricturing disease(45/169,26.6%)or stricturingtpenetrating disease(6/51,11.8%)(P=0.040).The rate of complications was higher among patients with diagnosis made at the time of surgery(15/31,48.4%)compared with the rest(85/331,25.7%)(P=0.013).Medication received at the time of surgery did not affect the rate of ***:Almost a quarter of patients developed early complications after intestinal *** disease and urgent surgery were associated with an increased risk of complications.

关键词： Ileocolonic resection Crohn’s disease post-operative complications risk factors

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Acquired Hemophilia A Simulating Retropharyngeal Abscess: Importance of Differential Diagnosis of Neck Masses before Surgery

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International Journal of Otolaryngology and Head & Neck Surgery 2015年第4期4卷 282-285页

作者： Isabel López-Sánchez José-Ramón Alba-García cristina Vázquez-romero Miguel Armengot-Carceller ENT Department General and University Hospital Valencia Spain Rhinology Unit ENT Department General and University Hospital.Valencia University Valencia Spain

The coexistence of acquired hemophilia A with a secondary retropharyngeal hematoma is an extremely unusual condition with important clinical implications. The purpose of this paper is to present a case involving a patient whose first clinical manifestation, namely dysphagia, along with specific clinical examination and imaging findings, led to an incorrect initial diagnosis of a retropharyngeal abscess. However, performance of a more thorough clinical examination led to the correct diagnosis of a hematoma secondary to acquired hemophilia A. This allowed surgery to be avoided in a patient at a high risk of bleeding. Conclusions: Acute neck masses require meticulous differential diagnosis assessing the possible presence of various causative systemic diseases before the most appropriate therapy can be determined.

关键词： Dysphagia Cervical Inflammation Coagulopathy Hematoma APTT Parapharyngeal Spaces

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Manipulation of bicarbonate concentration in sperm capacitation media improves in vitro fertilisation output in porcine species

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Journal of Animal Science and Biotechnology 2019年第2期10卷 375-389页

作者： cristina Soriano-úbeda Jon romero-Aguirregomezcorta Carmen Matás Pablo E.Visconti Francisco A.García-Vázquez Department of Physiology Faculty of Veterinary ScienceInternational Excellence Campus for Higher Education and Research“Campus Mare Nostrum”University of Murcia30100 MurciaSpain Institute for Biomedical Research of Murcia(IMIB-Arrixaca) MurciaSpain Department of Physiology Faculty of Medicine and NursingUniversity of the Basque Country UPV/EHULeioaBizkaiaSpain.4Department of Veterinary and Animal SciencesUniversity of MassachusettsAmherstMAUSA.

Background: The in vivo concentration of bicarbonate(HCO3-), one of the essential sperm capacitating effectors,varies greatly in the different environments sperm go through from cauda epididymis to the fertilisation site. On the contrary, porcine in vitro sperm capacitation and fertilisation media usually contains a standard concentration of25 mmol/L, and one of the main problems presented is the unacceptable high incidence of polyspermy. This work hypothesised that by modifying the HCO3-concentration of the medium, the output of in vitro sperm capacitation and fertilisation could be ***: Once exposed to the capacitation medium, the intracellular pH(pHi) of spermatozoa increased immediately even at low concentrations of HCO3-, but only extracellular concentrations of and above 15 mmol/L increased the substrates protein kinase A phosphorylation(pPKAs). Although with a significant delay, 15 mmol/L of HCO3-stimulated sperm linear motility and increased other late events in capacitation such as tyrosine phosphorylation(Tyr-P) to levels similar to those obtained with 25 mmol/L. This information allowed the establishment of a new in vitro fertilisation(IVF)system based on the optimization of HCO3-concentration to 15 mmol/L, which led to a 25.3% increment of the viable zygotes(8.6% in the standard system vs. 33.9%).Conclusions: Optimising HCO3-concentrations allows for establishing an IVF method that significantly reduced porcine polyspermy and increased the production of viable zygotes. A concentration of 15 mmol/L of HCO3-in the medium is sufficient to trigger the in vitro sperm capacitation and increase the fertilisation efficiency in porcine.

关键词： Adcy10 Boar Monospermy PKA

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COVID-19 ocular findings in children: a case series

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World Journal of Pediatrics 2021年第3期17卷 329-334页

作者： Celia Fernandez Alcalde Maria Granados Fernandez Maria Nieves Moreno cristina Calvo Rey Iker Fakes romero Susana Noval Martin Department of Ophthalmology Hospital Universitario La PazPaseo de la Castellana 26128046 Madrid28046 MadridMadridEspana Paediatric and Infectious Diseases Department Hospital Universitario La Paz and Fundacion IdipazMadridSpain Translational Research Network in Pediatric Infectious Diseases(RITIP) MadridSpain Microbiology and Parasitology Department Hospital Universitario La PazMadridSpain Universidad Autonoma de Madrid MadridSpain

Children account for 1%of cases of COVID-19 in most published series,with significantly reduced risk of developing severe forms of disease or death[1-3].Because most children present with mild forms of acute respiratory infections,interest has been focused initially on children with underly-ing/comorbid diseases and concentrated rare fatalities[1,4].

关键词： acute ocular respiratory

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Uncovering the multifaceted roles played by neutrophils in allogeneic hematopoietic stem cell transplantation

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Cellular & Molecular Immunology 2021年第4期18卷 905-918页

作者： cristina Tecchio Marco Antonio Cassatella Department of Medicine Section of Hem atology and Bone Marrow Transplant UnitUniversity of VeronaVeronaItaly Department of Medicine Section of General PathologyUniversity of VeronaVeronaItaly

Allogeneic hematopoietic stem cell transplantation(alloHSCT)is a life-saving procedure used for the treatment of selected hematological malignancies,inborn errors of metabolism,and bone marrow *** role of neutrophils in alloHSCT has been traditionally evaluated only in the context of their ability to act as a first line of defense against ***,recent evidence has highlighted neutrophils as key effectors of innate and adaptive immune responses through a wide array of newly discovered ***,neutrophils are emerging as highly versatile cells that are able to acquire different,often opposite,functional capacities depending on the microenvironment and their differentiation ***,we review the current knowledge on the multiple functions that neutrophils exhibit through the different stages of alloHSCT,from the hematopoietic stem cell(HSC)mobilization in the donor to the immunological reconstitution that occurs in the recipient following HSC *** also discuss the influence exerted on neutrophils by the immunosuppressive drugs delivered in the course of alloHSCT as part of graft-versushost disease(GVHD)***,the potential involvement of neutrophils in alloHSCT-related complications,such as transplant-associated thrombotic microangiopathy(TA-TMA),acute and chronic GVHD,and cytomegalovirus(CMV)reactivation,is also *** on the data reviewed herein,the role played by neutrophils in alloHSCT is far greater than a simple antimicrobial ***,much remains to be investigated in terms of the potential functions that neutrophils might exert during a highly complex procedure such as alloHSCT.

关键词： neutrophils allogeneic hematopoietic stem cell transplantation(alloHSCT) alloHSCT-associated complications lowdensity neutrophils(LDNs) normal-density neutrophils(NDNs)

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Serum response factor:Look into the gut

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World Journal of Gastroenterology 2010年第18期16卷 2195-2201页

作者： cristina Modak Department of Research(09-151) VA Long Beach Healthcare System

Serum response factor(SRF) is a transcription factor that regulates many genes involved in cellular activities such as proliferation,migration,differentiation,angiogenesis,and *** it has only been known for about two decades,SRF has been studied *** date,over a thousand SRF studies have been published,but it still remains a hot *** to its critical role in mesoderm-derived tissues,most of the SRF studies focused on muscle structure/function,cardiovascular development/maintenance,and smooth muscle generation/***,SRF has received more attention in the digestive field and several important discoveries have been *** review will summarize what we have learned about SRF in the gastrointestinal tract and provide insights into possible future directions in this area.

关键词： Angiogenesis Cell invasion Myofibroblast differentiation Smooth muscle contraction Serum response factor Wound healing

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S-adenosyl-methionine decreases ethanol-induced apoptosis in primary hepatocyte cultures by a c-Jun N-terminal kinase activity-independent mechanism

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World Journal of Gastroenterology 2006年第12期12卷 1895-1904页

作者： Maria del Pilar Cabrales-romero Lucrecia Márquez-Rosado Samia FatteI-Fazenda cristina Trejo-Solis Evelia Arce-Popoca Leticia Alemán-Lazarini Saúl Villa-Trevineo Department of Cell Biology Centro de Investigacién y Estudios Avanzados IPN Av. IPN No. 2508 México DF México Department of Neuroimmunology Instituto National de Neurologia y Neurocirugia MVS Insurgentes Sur 3877 Col La Fama DF México

AIM： To determine the role of c-Jun N-terminal kinase （JNK） activity in ethanol-induced apoptosis and the modulation of this signaling cascade by S-Adenosylmethionine （AdoMet）. METHODS： Primary hepatocyte cultures were pretreated with 100 IJmol/L SP600125, a selective JNK inhibitor, 1 mL/L DMSO or 4 mmol/L AdoMet and then exposed to 100 mmo/L ethanol. Hepatocyte apoptosis was determined by the TUNEL and DNA ladder assays. JNK activity and its inhibition by SP600125 and AdoMet were determined by Western blot analysis of c-jun phosphorylation and Bid fragmentation. SP600125 and AdoMet effects on the apoptotic signaling pathway were determined by Western blot analysis of cytochrome c release and pro-caspase 3 fragmentation. The AdoMet effect on glutathione levels was measured by EIIman＇s method and reactive oxygen species （ROS） generation by cell cytometry. RESULTS： The exposure of hepatocytes to ethanol induced JNK activation, c-jun phosphorylation, Bid fragmentation, cytochrome c release and pro-caspase 3 cleavage; these effects were diminished by SP600125, and caused a significant decrease in ethanol-induced apoptosis （P〈 0.05）. AdoMet exerted an antioxidant effect maintaining glutathione levels and decreasing ROS generation, without a significant effect on JNK activity, and prevented cytochrome c release and pro-caspase 3 cleavage.CONCLUSION： The JNK signaling cascade is a key component of the proapoptotic signaling pathway induced by ethanol. JNK activation may be independent from ROS generation, since AdoMet which exerted antioxidant properties did not have a significant effect on JNK activity. JNK pathway modulator agents and AdoMet may be components of promising therapies for alcoholic liver disease （ALD） treatment.

关键词： Alcoholic liver disease c-Jun N-terminal kinase Apoptosis SP600125 S-Adenosyl methionine Bid Reactive oxygen species

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Recent results from NA48/2

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Chinese Physics C 2010年第6期34卷 749-752页

作者： cristina Morales Morales Institut für Physik Universitt Mainz

Recent results on radiative K^± decays from the NA48/2 experiment are reported. From the full NA48/2 data set, about a million K^±→π^±π^γ decays were reconstructed. Based on this sample, the first measurement of the interference between direct photon emission and inner bremsstrahlung in K^±→π^±π^γ decays was performed. Stringent limits on CP violation in this decay were also set. In addition, a precise measurement of the branching fraction of K^±→π^±γγ is presented. This measurement was based on a data sample of more than 1000 event candidates. Also the related decay K^±→π^±e^＋e^-γ has first been observed. Results of the measurement of the decay rate and the decay parameter 5 are summarized.

关键词： radiative K^± decays NA48/2 CP violation

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