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Pathology Isolation and Identification of Canine Herpesvirus (CHV-1) in Mexico

Open Journal of Pathology 2016年第3期6卷 111-121页

作者： E. Guillermo Valdivia Lara Blanca Lilia Barrón Romero Laura Cobos Marín Jessica Ileana Ángeles Solis Sofía gonzález Gallardo Cesar Cuenca Verde Juan Antonio Montaraz crespo Guillermo Valdivia Anda Facultad de Estudios superiores Cuautitlán Universidad Nacional Autónoma de México Estado de México México Escuela Nacional de Ciencias Biológicas Instituto Politécnico Nacional Ciudad de México México Universidad Nacional Autónoma de México Ciudad Universitaria Ciudad de México México Facultad de Estudios superiores Cuautitlán Universidad Nacional Autónoma de México Estado de México México

This work presents the pathology description, isolation and identification of canine herpesvirus (CHV-1) in Mexico, a virus that causes a generalized hemorrhagic infection in puppies from the canidae family. Methods: Isolates were obtained from puppies that died within the first four weeks of life and had lesions consistent with canine herpesvirus. Results: The main gross lesions were petechial and ecchymotic hemorrhages in kidneys, liver and lungs;proliferative interstitial nephritis;multifocal necrosis in liver and kidneys;and encephalitis with intranuclear inclusion bodies. Herpesvirus was confirmed through direct immunofluorescence, electron microscopy and polymerase chain reaction for DNA polymerase and glycoprotein B genes. Discussion: Eight strains were isolated and identified as canine herpesvirus corresponding to three of the working cases with gross and microscopic lesions very similar to those described in the literature;then, isolates were confirmed by PCR gene amplification, positive reactions on immunofluorescence and observations from electron microscopy. This work represents the first report of this disease, including gross and histological lesions, and confirmation by isolation and identification of the canine herpesvirus in Mexico.

关键词： Pathology of Puppies Canine Herpesvirus PCR Electron Microscopy

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Lichenicolous fungi of the genus Abrothallus (Dothideomycetes: Abrothallales ordo nov.) are sister to the predominantly aquatic Janhulales

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Fungal Diversity 2014年第1期64卷 295-304页

作者： Sergio Pérez-Ortega Ave Suija Ana crespo Asunción de los Ríos Departamento Biología Ambiental Museo Nacional de Ciencias Naturales(CSIC)c/Serrano 115 dpdo28006 MadridSpain Institute of Ecology and Earth Sciences University of Tartu40 Lai str.51005 TartuEstonia Facultad de Farmacia Departamento Biología Vegetal IIUniversidad Complutense de MadridPlaza Ramón y Cajal s/n28040 MadridSpain

This study provides new insights on the phylogenetic position of the lichenicolous fungal genus Abrothallus based on six molecular markers(nuSSU,nuLSU,mtSSU,RPB1,RPB2 and TEF-α).In a broad-scale analysis,we detected high support for inclusion of the genus within Dothideomycetes.A further analysis provided support for Abrothallus as a member of the subclass Pleosporomycetidae as a sister group of Jahnulales,an order of aquatic *** the exclusive characters of this group of apotheciate fungi within the Dothidiomycetes,a new monotypic order Abrothallales is here introduced together with the new family *** a multi-locus analysis(based on the six loci indicated above plus ITS)restricted to 12 putative Abrothallus species,two clearly separated clades were observed:one comprising species growing on lichens of the families Parmeliaceae and Ramalinaceae,and the second including species that live on lichens of the order Peltigerales and the family Cladoniaceae.

关键词： Lichenicolous Multi-locus analysis Taxonomy Pleosporomycetidae Jahnulales

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New approach to assess sperm DNA fragmentation dynamics： Fine-tuning mathematical models

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Journal of Animal Science and Biotechnology 2017年第3期8卷 592-600页

作者： Isabel Ortiz Jesus Dorado Jane Morrell Jaime Gosalvez Francisco crespo Juan M.Jimenez Manuel Hidalgo Veterinary Reproduction Group Department of Animal Medicine and Surgery Faculty of Veterinary Medicine University of Cordoba 14071 Cordoba Spain Department of Clinical Sciences Division of Reproduction Swedish University of Agricultural Sciences Box 7054 SE-75007 Uppsala Sweden Department of Biology Universidad Aut6noma de Madrid 28049 Madrid Spain Department of Reproduction Centro Militar de Cria Caballar (FESCCR-Ministry of Defense) 05005 Avila Spain Department of Chemistry and Physics of Materials University of Salzburg Hellbrunner StraBe 34/111 A-5020 Salzburg Austria

Background： Sperm DNA fragmentation（sDF） has been proved to be an important parameter in order to predict in vitro the potential fertility of a semen sample. Colloid centrifugation could be a suitable technique to select those donkey sperm more resistant to DNA fragmentation after thawing. Previous studies have shown that to elucidate the latent damage of the DNA molecule, sDF should be assessed dynamically, where the rate of fragmentation between treatments indicates how resistant the DNA is to iatrogenic damage. The rate of fragmentation is calculated using the slope of a linear regression equation. However, it has not been studied if s DF dynamics fit this model. The objectives of this study were to evaluate the effect of different after-thawing centrifugation protocols on sperm DNA fragmentation and elucidate the most accurate mathematical model（linear regression, exponential or polynomial） for DNA fragmentation over time in frozen-thawed donkey ***： After submitting post-thaw semen samples to no centrifugation（UDC）, sperm washing（SW） or single layer centrifugation（SLC） protocols, sD F values after 6 h of incubation were significantly lower in SLC samples than in SW or *** of determination（R-2） values were significantly higher for a second order polynomial model than for linear or exponential. The highest values for acceleration of fragmentation（aSDF） were obtained for SW, fol owed by SLC and ***： SLC after thawing seems to preserve longer DNA longevity in comparison to UDC and SW. Moreover,the fine-tuning of models has shown that sDF dynamics in frozen-thawed donkey semen fit a second order polynomial model, which implies that fragmentation rate is not constant and fragmentation acceleration must be taken into account to elucidate hidden damage in the DNA molecule.

关键词： Colloid centrifugation Dynamics Fine-tuning Mathematical models Sperm DNA fragmentation

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Vitamin D deficiency in chronic liver disease

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World Journal of Hepatology 2014年第12期6卷 901-915页

作者： Paula Iruzubieta lvaro Terán Javier crespo Emilio Fábrega Gastroenterology and Hepatology Unit Marqués de Valdecilla University Hospital Instituto de Investigación Marqués de Valdecilla 39008 Santander Cantabria Spain

Vitamin D is an important secosteroid hormone with known effect on calcium homeostasis,but recently there is increasing recognition that vitamin D also is involved in cell proliferation and differentiation,has immunomodulatory and anti-inflammatory *** D deficiency has been frequently reported in many causes of chronic liver disease and has been associated with the development and evolution of non-alcoholic fatty liver disease(NAFLD)and chronic hepatitis C(CHC)virus *** role of vitamin D in the pathogenesis of NAFLD and CHC is not completely known,but it seems that the involvement of vitamin D in the activation and regulation of both innate and adaptive immune systems and its antiproliferative effect may explain its importance in these liver *** studies provide evidence for routine screening for hypovitaminosis D in patients with liver *** prospectives studies demonstrating the impact of vitamin D replacement in NAFLD and CHC are required.

关键词： Cholecalciferol Vitamin D Hepatitis C Liver fibrosis Liver disease Interferon Sustained virological response Nonalcoholic fatty liver disease Nonalcoholic steatohepatitis

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Clinical significance of donor-specific human leukocyte antigen antibodies in liver transplantation

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World Journal of Gastroenterology 2015年第39期21卷 11016-11026页

作者： Antonio Cuadrado David San Segundo Marcos López-Hoyos Javier crespo Emilio Fábrega Gastro- enterology and Hepatology Unit Marqués de Valdecilla University Hospital Instituto de Investigación Marqués de Valdecilla Immunology Unit Marqués de Valdecilla University Hospital. Instituto de Investigación Marqués de Valdecilla

Antibody-mediated rejection(AMR) caused by donorspecific anti-human leukocyte antigen antibodies(DSA) is widely accepted to be a risk factor for decreased graft survival after kidney transplantation. This entity also plays a pathogenic role in other solid organ transplants as it appears to be an increasingly common cause of heart graft dysfunction and an emerging issue in lung transplantation. In contrast, the liver appears relatively resistant to DSA-mediated injury. This "immune-tolerance" liver property has been sustained by a low rate of liver graft loss in patients with preformed DSA and by the intrinsic liver characteristics that favor the absorption and elimination of DSA; however, alloantibody-mediated adverse consequences are increasingly being recognized, and several cases of acute AMR after ABO-compatible liver transplant(LT) have been reported. Furthermore, the availability of new solid-phase assays, allowing the detection of low titers of DSA and the refinement of objective diagnostic criteria for AMR in solid organ transplants and particularly in LT, have improved the recognition and management of this entity. A cost-effective strategy of DSA monitoring, avoidance of class Ⅱ human leukocyte antigen mismatching, judicious immunosuppression attached to a higher level of clinical suspicion of AMR, particularly in cases unresponsive to conventional antirejection therapy, can allow a rational approach to this threat.

关键词： Donor-specific anti-human leukocyte antigen antibo

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Carbon isotopes of graphite:Implications on fluid history

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Geoscience Frontiers 2012年第2期3卷 197-207页

作者： F.J.Luque E.crespo-Feo J.F.Barrenechea L.Ortega Departamento de cristalografía y Mineralogía Facultad de Geología Universidad Complutense de Madrid 28040 MadridSpain Instituto de Geociencias(UCM-CSIC) Jos Antonio Novais 228040 MadridSpain Departamento de cristalografia y Mineralogia Facultad de Geología Universidad Complutense de Madrid28040 MadridSpain Departamento de cristalografía y Mineralogía Facultad de Geología Universidad Complutense de Madrid28040 MadridSpain

Stable carbon isotope geochemistry provides important information for the recognition of funda- mental isotope exchange processes related to the movement of carbon in the lithosphere and permits the elab- oration of models for the global carbon cycle. Carbon isotope ratios in fluid-deposited graphite are powerful tools for unravelling the ultimate origin of carbon （organic matter, mantle, or carbonates） and help to constrain the fluid history and the mechanisms involved in graphite deposition. Graphite precipitation in fluid-deposited occurrences results from C02- and/or CH4-bearing aqueous fluids. Fluid flow can be considered as both a closed （without replenishment of the fluid） or an open system （with renewal of the fluid by successive fluid batches）. In closed systems, carbon isotope systematics in graphite is mainly governed by Rayleigh precipi- tation and/or by changes in temperature affecting the fractionation factor between fluid and graphite. Such processes result in zoned graphite crystals or in successive graphite generations showing, in both cases, isotopic variation towards progressive 13C or 12C enrichment （depending upon the dominant carbon phase in the fluid, C02 or CH4, respectively）. In open systems, in which carbon is episodically introduced along the fracture systems, the carbon systematics is more complex and individual graphite crystals may display oscillatory zoning because of Rayleigh precipitation or heterogeneous variations of 613C values when mixing of fluids or changes in the composition of the fluids are the mechanisms responsible for graphite precipitation.

关键词： Graphite Carbon isotopes Crust C-O-H fluids

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Out of Africa: Miocene Dispersal, Vicariance, and Extinction within Hyacinthaceae Subfamily Urgineoideae

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Journal of Integrative Plant Biology 2013年第10期55卷 950-964页

作者： Syed Shujait Ali Martin Pfosser Wolfgang Wetschnig Mario Martínez Azorín Manuel B.crespo Yan Yu Institute of Plant Sciences Karl Franzens University Graz Biocenter Linz Institute of Biotechnology and Microbiology University of Swat CIBIO (Instituto Universitario de la Biodiversidad) Universidad de Alicante Key Laboratory of Bio Resources and Eco Environment of Ministry of Education College of Life Sciences Sichuan University

Disjunct distribution patterns in plant lineages are usually explained according to three hypotheses： vicariance, geodispersal, and long-distance dispersal. The role of these hypotheses is tested in Urgineoideae （Hyacinthaceae）, a subfamily disjunctly distributed in Africa, Madagascar, India, and the Mediterranean region. The potential ancestral range, dispersal routes, and factors responsible for the current distribution in Urgineoideae are investigated using divergence time estimations. Urgineoideae originated in Southern Africa approximately 48.9 Mya. Two independent dispersal events in the Western Mediterranean region possibly occurred during Early Oligocene and Miocene （29.9-8.5 Mya） via Eastern and Northwestern Africa. A dispersal from Northwestern Africa to India could have occurred between 16.3 and 7.6 Mya. Vicariance and extinction events occurred approximately 21.6 Mya. Colonization of Madagascar occurred between 30.6 and 16.6 Mya, after a single transoceanic dispersal event from Southern Africa. The current disjunct distributions of Urgineoideae are not satisfactorily explained by Gondwana fragmentation or dispersal via boreotropical forests, due to the younger divergence time estimates. The flattened winged seeds of Urgineoideae could have played an important role in long- distance dispersal by strong winds and big storms, whereas geodispersal could have also occurred from Southern Africa to Asia and the Mediterranean region via the so-called arid and high-altitude corridors.

关键词： Bayesian binary method Bayesian divergence estimates biogeography disjunct distribution time-event curve Urgineoideae.

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A model to determining the remaining useful life of rotating equipment,based on a new approach to determining state of degradation

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Journal of Central South University 2020年第8期27卷 2291-2310页

作者： Saeed RAMEZANI Alireza MOINI Mohamad RIAHI Adolfo crespo MARQUEZ Department of Industrial Engineering Iran University of Science and TechnologyTehranIran Department of Mechanical Engineering Iran University of Science and TechnologyTehranIran Department of Industrial Management University of SevilleSpain

Condition assessment is one of the most significant techniques of the equipment’s health ***,in PHM methodology cycle,which is a developed form of CBM,condition assessment is the most important step of this *** this paper,the remaining useful life of the equipment is calculated using the combination of sensor information,determination of degradation state and forecasting the proposed health *** combination of sensor information has been carried out using a new approach to determining the probabilities in the Dempster-Shafer combination rules and fuzzy c-means clustering *** the simulation and forecasting of extracted vibration-based health index by autoregressive Markov regime switching(ARMRS)method,final health state is determined and the remaining useful life(RUL)is *** order to evaluate the model,sensor data provided by FEMTO-ST Institute have been used.

关键词： remaining useful life(RUL) prognostics and health management(PHM) autoregressive markov regime switching(ARMRS) health index(HI) Dempster-Shafer theory fuzzy c-means(FCM) Kurtosis-entropy degradation

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Risk Stratification in Obstetrics: An Integrated Approach to Maternal Health

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Open Journal of Obstetrics and Gynecology（妇产科期刊） 2024年第6期 903页

作者： Taina crespo Mendonca Bianca Beatriz Soares dos Reis Júlia Cruz Coelho Antônio de Oliveira Battistini Pestana Vitória Carolina Barreto Negri Alvaro Augusto Trigo

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Using a temporal phylogenetic method to harmonize familyand genus-level classification in the largest clade of lichen-forming fungi

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Fungal Diversity 2017年第3期84卷 101-117页

作者： Pradeep K.Divakar Ana crespo Ekaphan Kraichak Steven D.Leavitt Garima Singh Imke Schmitt H.Thorsten Lumbsch Departamento de Biología Vegetal II Facultad de FarmaciaUniversidad Complutense de Madrid28040 MadridSpain Department of Botany Faculty of ScienceKasetsart UniversityBangkokThailand Department of Biology and M.L.Bean Life Science Museum Brigham Young University4102 Life Science BuildingProvoUT 84602USA Department of Biological Sciences Institute of EcologyEvolution and DiversityGoethe Universitat and Senckenberg Biodiversity and Climate Research Centre(BiK-F)Frankfurt am MainGermany Science&Education The Field MuseumChicagoILUSA

Although classification at supra-specific ranks is inherently arbitrary,comparable taxonomic ranks within clades can facilitate more consistent classifications and objective comparisons among *** circumscriptions of the hyper-diverse lichen-forming fungal family Parmeliaceae and widely different generic circumscriptions among authors have been *** this study,we use a recently developed temporal approach that uses timecalibrated chronograms to identify temporal bands for specific ranks in Parmeliaceae and allied groups with the overarching goal of establishing a consistent,stable classification.A data set of 330 species,representing 73 genera in the family and 52 species of related families was used to address the circumscription of Parmeliaceae and its genera following the proposed temporal *** on the results of this study,we propose a revised,temporal-based classification for Parmeliaceae,including all clades that share a common ancestor 102.13–112.88 Ma for families and a time window of 29.45–32.55 Ma for *** of the currently accepted genera in Parmeliaceae were supported in their current *** subfamilies are accepted within Parmeliaceae:Protoparmelioideae Divakar et ***.,including Protoparmelia and the resurrected genus Maronina,and Parmelioideae,including the bulk of genera in the *** new genus Austromelanelixia Divakar et *** proposed to accommodate a clade of southern Hemisphere species previously included in *** and tentatively Dolichousnea are resurrected as genera separate from *** following genera are reduced to synonymy:Allocetraria,Cetrariella,Usnocetraria,and Vulpicida with Cetraria;Arctocetraria,Cetreliopsis,Flavocetraria,Kaernefeltia,Masonhalea,Tuckermanella,and Tuckermannopsis with Nephromopsis;and the lichenicolous genera Nesolechia and Raesaenenia with the lichen-forming genera Punctelia and Protousnea,respectively.A total of 47 new combinations and

关键词： Ascomycota Gypsoplacaceae Lecanorales Lichenized fungi Parmeliaceae Phylogeny Protoparmelia Taxonomy Taxonomicranks

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