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An Efficient Approach to 6, 7-Disubstituted-1H-quinoxalin-2-ones

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Chinese Chemical Letters 2004年第12期15卷 1400-1402页

作者： Xun LI Qing Ping HU Xue Gui CUI Dong Hua WANG college of pharmacy, Shandong University, Jinan 250100 college of Pharmaceuticals and Biotechnology, Tianjin University, Tianjin 300072 college of pharmacy Shandong University Jinan 250100 China Coll. of Pharmaceut. and Biotech. Tianjin University Tianjin 300072 China

A novel approach to the synthesis of 6, 7-disubstituted-1H-quinoxalin-2-ones is *** title compounds were regioselectively prepared by starting from substituted phenylamines andchloroacetyl chloride through the efficie... 详细信息

关键词： 6 7 -Disubstituted-1H-quinoxalin-2-ones regioselective substituted phenylamine.

Immune tolerance induced by adoptive transfer of dendritic cells in an insulin-dependent diabetes mellitus murine model

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Acta Pharmacologica Sinica 2007年第1期28卷 98-104页

作者： Cheng-liang ZHANG Xiao-lei ZOU Jia-bei PENG Ming XIANG~2 Department of Pharmacology college of pharmacy Tongji Medical college,Huazhong University of Science and Technology,Wuhan 430030,China Department of Pharmacology College of Pharmacy Tongji Medical College Huazhong University of Science and Technology Wuhan China

Aim:To investigate the effect and underlying mechanisms of immune-toleranceinduced by the adoptive transfer of bone marrow(BM)-derived dendritic cells(DC)in insulin-dependent diabetes mellitus(IDDM)***:The IDDMmodel was established by a low close of streptozotocin(STZ)in Balb/c *** subpopulations were generated from the BM cells with granulocyte-macroph-age colony-stimulating factor with or without *** purity and the Tcell stimulatory capability of DC were *** cells were used to modu-late autoimmune response in pre-diabetic *** glucose was examinedweekly;pancreas tissues were taken for histopathological analysis,and CD4~+ Tcells were isolated to detect lymphocyte proliferation by MTT assay and the ratioof CD4~+CD25+ T cells by fluorescence-activated cell sorting(FACS).The cytokinesecretion was determined by ELISA ***:Two DC subsets weregenerated from BM,which have phenotypes of mature DC(mDC)and immatureDC(iDC),*** level of blood glucose decreased significantly bytransferring iDC(P<0.01)rather than *** lymphocyte infiltration was ob-served in the islets,and pancreatic structure was *** vitro,proliferation oflymphocytes decreased and the proportion of CD4~+CD25~+ T cells increasedremarkably,compared with the mDC-treated groups(P<0.05),which were associ-ated with increased level of the Th2 cytokine and reduced level of the Th 1 cytokineafter iDC ***:Our data showed that iDC transfer was able toconfer protection to mice from STZ-induced *** immune-tolerance toIDDM may be associated with promoting the production of CD4~+CD25~+ T cellsand reducing regulatory Th2 responses in vivo.

关键词： dendritic cells insnlin-dependent diabetes mellitus streptozotocin immune tolerance regulatory T cells

Metabolites of Icariin in Urine Following Oral Administration

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Chinese Chemical Letters 1998年第4期9卷 393-395页

作者： Feng QIU Ying Jie CHEN Yoshihiro KANO Xin Sheng YAO(Shenyang Pharmaceutical University, Shenyang 110015 (Hokkaido college of pharmacy, Japan) Shenyang Pharmaceutical University Shenyang 110015 China Hokkaido college of pharmacy Japan

Urinary metabolites of icariin, present in Epimedium, were investigated using rats. In the urine of rats administered icariin orally, two major metabolites were detected, which were identified as icariside II and icar... 详细信息

关键词： icariin icariside II icaritin metabolite

New diterpenoids from Isodon excisoides

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Chinese Chemical Letters 2010年第5期21卷 610-612页

作者： Yong Xue Wang,Lin Lin Zhu,Zhi An He,Ji Xia Zhang pharmacy college,Xinxiang Medical University,Xinxiang 453003,China pharmacy college Xinxiang Medical University Xinxiang 453003 China

Two new ent-kaurane diterpenoids,taihangexcisoidesin C(1) and its acetonide,taihangexcisoidesin D(2) were isolated from the leaves of Isodon *** structures were determined by 1D and 2D spectroscopic analysis.

关键词： Isodon excisoides Ent-kaurane Diterpenoid Taihangexcisoidesin C and D

Morroniside protects cultured human umbilical vein endothelial cells from damage by high ambient glucose

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Acta Pharmacologica Sinica 2004年第4期25卷 14-17页

作者： Hui-qin XU2, Hai-ping HAO2, Xu ZHANG3,Yang PAN2 2college of pharmacy 3BasicMedicine college, Nanjing Universityof Traditional ChineseMedicine, Nanjing 210029, China college of pharmacy Nanjing University of Traditional Chinese Medicine Nanjing 210029 Basic Medicine college Nanjing University of Traditional Chinese Medicine Nanjing 210029

AIM: To determine whether morroniside, a compound in Cornus officinalis Sieb et Zucc can prevent cultured human umbilical vein endothelial cells (HUVEC) from damage by high ambient glucose. METHODS: HUVEC was incubated in glucose, 5 or 30 mmol/L, either alone or in the presence of morroniside (final concentration 100, 10, and 1 μmol/L, respectively) for 48 h. The proliferation of HUVEC was quantified by MTT method; its cycle was analyzed by flow cytometry; morphological change was observed with fluorescence microscopy. RESULTS: Survival of HUVEC cultured in high ambient glucose was significantly decreased when compared to that in normal concentration of glucose (P<0.01). High ambient glucose also lowered the rate of cells entering into S-phase, along with severe morphological damage. With the intervention of morroniside (final concentration 100 and 10 μmol/L), the cell survival was significantly recovered (P<0.01, P<0.05, respectively), accompanied with increased S-phase rate and less extent of morphological damage. CONCLUSION: Morroniside protected HUVEC against high ambi- ent glucose induced injury, which suggested that morroniside could exert a beneficial effect on preventing diabetic angiopathies.

关键词： morroniside umbilical veins endothelial cells diabetic angiopathies

A new pteridinedione from Whitmania pigra

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Chinese Chemical Letters 2010年第5期21卷 603-605页

作者： Yun Feng Zheng,Cun Yu Li,Guo Ping Peng,Hong Yang Li college of pharmacy,Nanjing University of Chinese Medicine,Nanjing 210046,China college of pharmacy Nanjing University of Chinese Medicine Nanjing 210046 China

A new compound,6-methylsulfinylthieno[3,2-g]-2,4(1H,3H)-pteridinedione,was isolated from the water extract of Whitmania pigra *** structure was elucidated on the basis of NMR and ESIMS spectra.

关键词： Whitmania pigra Heterocycle 6-Methylsulfinylthieno[3,2-g]-2,4(1H,3H)-pteridinedione

SOLUTION PROPERTIES OF ANTITUMOR CARBOXYMETHYLATED DERIVATIVES OF α-(1→3)-D-GLUCAN FROM GANODERMA LUCIDUM

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Chinese Journal of Polymer Science 2001年第3期19卷 283-289页

作者： Li-na Zhang Mei Zhang Jing-hua Chen Fan-bo Zeng Department of Chemistry Wuhan University, Wuhan 430072, China pharmacy college, Tongji Medical University, Wuhan 430072, China 武汉大学湖北武汉同济医科大学湖北武汉

Fractions of a water insoluble α-(1→3)-D-glucan (GL) extracted from Ganoderma lucidum were carboxy-methylated (CM) to obtain water-soluble carboxymethylated derivatives (CM-GL) having a degree of substitution (DS) of0.38～0.51. Weight-average molecular weight M_w and intrinsic viscosity [η] of the samples CM-GL were measured by gelpermeation chromatography combined with laser light scattering (GPC-LLS) and viscometry. The CM-GL exhibits a stifferchain in aqueous solution at 25℃ than the original glucan, The antitumor activities against Ehrlich ascites carcinoma (EAC,5×10~6) of the carboxymethylated derivatives from the α-glucan and curdlan, a β-glucan, are significantly higher than thoseof the original glucans. The effects of the relatively low molecular weight, expanded chains and better water-solubility of theCM-GL on the enhancement of antitumor activity could not be neglected. The chain stiffness decreased speedily withincrease of temperature from 40 to 60℃ or NaOH concentration from 0.1 to 0.4 in the solution, respectively, and the changeof the chain stiffness is reversible.

关键词： Solution properties Antitumor activity Carboxymethylated derivative α-D-glucan Ganoderma lucidum

Metabolites of Icariin in Rat Bile Following Oral Administration

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Chinese Chemical Letters 1998年第6期9卷 549-552页

作者： Feng QIU Qing Song TAN Ying Jie CHEN Yoshihiro KANO Xin Sheng YAO(Shenyang Pharmaceutical University, Shenyang 110015Hokkaido college of pharmacy, Japan) Shenyang Pharmaceutical University Shenyang 110015 China Hokkaido college of pharmacy Japan

The biliary metabolites of orally administered icariin in rats were investigated. Two metabolites B-l and B-2 were isolated and identified as icaritin 3-O-alpha-L-rhamnopyranosyl-7-O-beta-D-glucopyranuronoside and ica... 详细信息

关键词： icariin icariside II icaritin 3-O-alpha-L-rhamnopyranosyl-7-O-beta-D-glucopyranuronoside metabolite

Effect of heparin on apoptosis in human nasopharyngeal carcinoma CNE2 cells

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Cell Research 2001年第4期11卷 311-315页

作者： LI HONG LIANG , KAI HE YE , HAI WEI ZHANG , YING RU LUO , XIAN DA REN, AI HUA XIONG, RUI SITU Department of Pharmacology ,pharmacy college, Department of Pathology Medical college, Jinan University, Guangzhou 510632, China Department of Pharmacology Pharmacy College Jinan University Guangzhou China Department of Pathology Medical college Jinan University Guangzhou China

In order to study the mechanism of the effect of heparin on apoptosis in carcinoma cells, the nasopha- ryngeal carcinoma cell line CNE2 was used to identify the effect of heparin on apoptosis associated with the expression of c-myc,bax, bcl-2 proteins by use of Hoechst 33258 staining, terminal deoxynucleotidyl transferasemediated dUTP nick-end labeling (TUNEL), agarose gel electrophoresis, and flow cytometry, as well as Western blot analysis. The results showed that heparin induced apoptosis of cNE2 cens including the morphologic changes such as reduction in the volume, and the nuclear chromatin condensation, as well as the 'ladder pattern' revealed by agarose gel electrophoresis of DNA in a concentration-dependent manner. The number of TUNEL-positive cells was dramatically increased to 33.6+1.2% from 2.8+0.3% by treat- ment with heparin in different concentrations (10~40 kU/L). The apoptotic index was increased to 32.5% from 3.5% by detecting SubG1 peaks on flow cytometry. Western blot analysis showed that levels of bcl-2, bax and c-myc were significantly overexpressed by treatment with the increase of heparin concentrations. These results suggest that heparin induces apoptosis of CNE2 cells, which may be regulated by differential expression of apoptosis-related genes.

关键词： Heparin agarose gel electrophoresis flow cytometry apoptosis protein kinase C(PKC)