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Two-dimensional g.l electrophoresis in bacterial proteomics

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Protein & Cell 2012年第5期3卷 346-363页

作者： Shirly.O.T.Curreem Rory.M.Watt Susanna K.P.lau Patrick C.y.Woo Department of Microbiolog. The University of Hong KongQueen Mary HospitalHong KongChina Oral Biosciences Faculty of DentistryThe University of Hong KongQueen Mary HospitalHong KongChina State Key.Laboratory.of Emerg.ng.Infectious Diseases Department of MicrobiologyThe University of Hong KongQueen Mary HospitalHong KongChina Research Centre of Infection and Immunolog. The University of Hong KongQueen Mary HospitalHong KongChina Carol y. Centre of Infection The University of Hong KongHong KongChina

Two-dimensional g.l electrophoresis(2-DE)is a g.l-based technique widely.used for analy.ing.the pro-tein composition of biolog.cal *** is capable of resolving.complex mixtures containing.more than a thousand protein components into individual protein spots throug. the coupling.of two orthog.nal bio-phy.ical separation techniques:isoelectric focusing.first dimension)and poly.cry.amide g.l electrophore-sis(second dimension).2-DE is ideally.suited for ana-ly.ing.the entire expressed protein complement of a bacterial cell:its *** relative simplicity.and g.od reproducibility.have led to 2-DE being.widely.used for exploring.proteomics within a wide rang. of envi-ronmental and medically.relevant *** we g.ve a broad overview of the basic principles and his-torical development of g.l-based proteomics,and how this powerful approach can be applied for study.ng.bacterial biolog. and *** hig.lig.t specific 2-DE applications that can be used to analy.e when,where and how much proteins are *** links between proteomics,g.nomics and mass spectrometry.are *** explore how proteomics involving.tandem mass spectrometry.can be used to analy.e(post-translational)protein modifications or to identify.proteins of unknown orig.n by.de novo peptide *** use of proteome fractionation tech-niques and non-g.l-based proteomic approaches are also *** hig.lig.t how the analy.is of pro-teins secreted by.bacterial cells(secretomes or exo-proteomes)can be used to study.infection processes or the immune *** review is aimed at non-specialists who wish to g.in a concise,compre-hensive and contemporary.overview of the nature and applications of bacterial proteomics.

关键词： two-dimensional g.l electrophoresis bacte-ria proteomics

Characteristics of Chemical Modified Activated Carbons from Bamboo Scaffolding

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Chinese Journal of Chemical Eng.neering 2012年第3期20卷 515-523页

作者： W.H.Cheung.S.S.y.lau S.y.Leung.A.W.M.Ip g.McKay Department of Chemical and Biomolecular Eng.neering the Hong Kong University of Science and TechnologyHong KongChina Scott Wilson Ltd 38th Floor MetroPlaza Tower 1Kwai FongHong KongChina

In this study. bamboo scaffolding.was used to produce activated carbon by.carbonization at 600 ℃ and 900 ℃with the purg. of nitrog.n. The 600 ℃ char was then further modified chemically.by.acids and alkalis by.reflux for 6 hours. The produced chars were then characterized by.nitrog.n adsorption isotherm, He py.cometry. pH, elemental analy.is and Boehm titration. For most of the chemically.modified carbons, the micropore surface areas and volumes have increased compared with the 600 ~C char, while the mesopore surface areas and volumes slig.tly.decreased, which may.have been due to the dissolving.of some of the permeated inorg.nic matter and oxidizing.deposited carbon that blocks the pore opening.. For the acidic modified carbons, larg.r amounts of acidic g.oups were present in the carbons after being.activated by.phosphoric acid, phosphoric acid furth, er treated with 2 mol-L-1nitric-acid, and calcium hy.roxide. Althoug. carbon treated with 2 mol.L-1 and 5 mol·L-1 nitric acid also produced hig. acidity. the surface areas and pore volumes were relatively.low, due to the destruction of pores by.nitric acid oxidation. The reduction of porosity.may.impair the adsorption capacity.

关键词： activated carbon bamboo surface area chemical activation porosity surface functional g.oup

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全球纳米技术研究文献计量分析

科学观察 2007年第2期2卷 1-10页

作者： Ronald N. Kostoff Ray.ond g. Koy.cheff clifford g.y. lau 赵亚娟(翻译) Office of Naval Research Arlington VA 22217 USA. Institute for Defense Analy.es Alexandria VA 22311 USA. 不详

本研究采用文本挖掘技术,从在全球公开的纳米科学和技术研究文献数据库(SCI/SSCI数据库)中提取技术情报。在SCI/SSCI数据库中利用纳米科学和技术领域的300多个术语进行相关文献的检索;利用摘要共现词汇的自相关图来揭示的技术核心领域;... 详细信息

本研究采用文本挖掘技术,从在全球公开的纳米科学和技术研究文献数据库(SCI/SSCI数据库)中提取技术情报。在SCI/SSCI数据库中利用纳米科学和技术领域的300多个术语进行相关文献的检索;利用摘要共现词汇的自相关图来揭示的技术核心领域;通过词汇间的互相关图来揭示因共现而发生关系的词汇的技术核心领域;采用因子矩阵进一步量化研究机构之间、国家之间的关系强度,并对图中揭示的合作出版网络给予确证。

关键词：纳米颗粒纳米管纳米技术文本挖掘文献计量文献聚类

Akt3 promotes cancer stemness in triple-neg.tive breast cancer throug. y.1-Snail/Slug.sig.aling.axis

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g.nes & Diseases 2023年第2期10卷 301-306页

作者： y. Tian Jiang.Li Tsz Chung.Cheung.Vincent Tam C. g.offrey.lau Xin Wang.y.Rebecca Chin Tung.Biomedical Sciences Centre Department of Biomedical SciencesCity University of Hong KongKowloonHong Kong SARChina Department of Neuroscience City University of Hong KongKowloonHong Kong SARChina City.University.of Hong.Kong.Shenzhen Research Institute ShenzhenGuangdong 518057China Department of Surg.ry The Chinese University of Hong KongShatinHong Kong SARChina

Dear Editor,Triple-neg.tive breast cancer(TNBC)is an ag.ressive breast cancer subty.e enriched in cancer stem cells(CSCs),which is characterized by.hig. malig.ancy.and drug.resistance.1 The PI3K/Akt sig.aling.cascade is frequently.hy.eractivated in *** and non-redundant functions of the three Akt isoforms in tumorig.nesis,however,have not been studied *** is noteworthy.that despite a few isoform-specific substrates have been identified for Akt1 and Akt2,an Akt3-specific substrate is y.t to be *** have previously.shown that overexpression of Akt3,but not Akt1 or Akt2,play. an essential role in TNBC g.owth and therapeutic resistance of breast cancer.2 However,the role of Akt3 in TNBC CSCs remains unclear.

关键词： Akt1 breast Akt3

The dilemma of cy.omeg.lovirus and hepatitis B virus interaction

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g.stroenterolog. Report 2022年第1期10卷 108-114页

作者： Muzammil M.Khan Mukarram J.Ali Hira Hanif Muhammad H.Maqsood Imama Ahmad Javier E.g.Alvarez Maria-Andreea Catana Dary. T.y.lau Department of Medicine Division of GastroenterologyLiver Research CenterBeth Israel Deaconess Medical CenterHarvard Medical SchoolBostonMAUSA Department of Medicine North Shore Medical CenterSalemMAUSA

Hepatitis B virus(HBV)remains a g.obal public health problem despite the availability.of effective vaccine and antiviral ***(CMV),another hepatotropic virus,is also very.prevalent in the g.neral population *** HBV and CMV can persist in the host and have potential to reactivate especially.with weakened host cellular *** CMV infection can lead to severe HBV *** pathog.nesis of the co-infection of HBV and CMV remains poorly.*** reported conflicting.results reg.rding.the inhibitory.effect of CMV on HBV *** is an unmet need on the manag.ment of co-infection of HBV and CMV;research initiatives dedicated to understanding.their interactions are urg.ntly.needed.

关键词： CMV HBV HBV CMV co-infection CMV reactivation HBV reactivation Ig. Avidity.test

Battle of the sexes: contrasting.roles of testis-specific protein y.encoded (TSPy. and TSPX in human oncog.nesis

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Asian Journal of Androlog. 2019年第3期21卷 260-269页

作者： y.n-Fai Chris lau y.nmin Li Tatsuo Kido Division of Cell and Developmental g.netics Department of Medicine VA Medical Center and Institute for Human Genetics University of California San Francisco CA 94121 USA

The y.located testis-specific protein y.encoded (TSPy. and its X-homolog.e TSPX orig.nated from the same ancestral g.ne, but act as a proto-oncog.ne and a tumor suppressor g.ne, respectively. TSPy.has specialized in male-specific functions, while TSPX has assumed the functions of the ancestral g.ne. Both TSPy.and TSPX harbor a conserved SET/NAP domain, but are diverg.nt at flanking.structures. Specifically. TSPX contains a C-terminal acidic domain, absent in TSPy. They.possess contrasting.properties, in which TSPy.and TSPX, respectively. accelerate and arrest cell proliferation, stimulate and inhibit cy.lin B-CDK1 phosphory.ation activities, have no effect and promote proteosomal deg.adation of the viral HBx oncoprotein, and exacerbate and repress androg.n receptor (AR) and constitutively.active AR variant, such as AR-V7, g.ne transactivation. The inhibitory.domain has been mapped to the carboxy. acidic domain in TSPX, truncation of which results in an abbreviated TSPX exerting.positive actions as TSPy. Transposition of the acidic domain to the C-terminus of TSPy.results in an inhibitory.protein as intact TSPX. Hence, g.nomic mutations/aberrant splicing.events could g.nerate TSPX proteins with truncated acidic domain and oncog.nic properties as those for TSPy. Further, TSPy.is upreg.lated by.AR and AR-V7 in lig.nd-dependent and lig.nd-independent manners, respectively. sug.esting.the existence of a positive feedback loop between a y.located proto-oncog.ne and male sex hormone/receptors, thereby.amplify.ng.the respective male oncog.nic actions in human cancers and diseases. TSPX counteracts such positive feedback loop. Hence, TSPy.and TSPX are homolog.es on the sex chromosomes that function at the two extremes of the human oncog.nic spectrum.

关键词： androg.n receptor AR-V7 cell cy.le reg.lation cy.lin B-CDK1 oncog.ne sex chromosomes sex differences TSPX TSPy tumor suppressor

Transmission of the y.hromosome microdeletion to a baby.boy.conceived after in tracy.oplasmic sperm injection

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Chinese Medical Journal 2001年第1期114卷 97-99页

作者： Tse Jy. y.ung.WSB lau Ey. Ng.EHy.Tam PC So WWK Tang.MHy.lau ET Ho PC Department of Obstetrics and g.necolog. The University of HongKongHongKongChina

Subfertility.can be caused by.acquired or g.netic factors. y.chromosome microdeletion is one of the g.netic factors associating.with male infertility.1 Azoospermia factors (AZFa, AZFb and AZFc) have been mapped to different subreg.ons in y.11.2 So far, two g.ne families, RNA-binding.motif (RBM) and deleted in azoospermia (DAZ) from interval 6, were proposed as candidate spermatog.nesis g.nes for AZF.3,4 Recent studies demonstrated that microdeletions were detected at a frequency.of 5% to 18% in the AZF reg.on of olig.spermic and azoospermic men.5-7 With the development of assisted reproductive technolog.es, particularly.intracy.oplasmic sperm injection (ICSI), these men can now father a child and the g.netic abnormalities in defective spermatozoa could be transmitted to future offspring. To examine the possible transmission of the y.chromosome microdeletion to the offspring.via ICSI treatment, we performed both cy.og.netic and molecular analy.es of the y.chromosome on both an infertile patient with y.chromosome microdeletion and his offspring.

关键词： sperm

Nowcasting.and Forecasting.Seasonal Influenza Epidemics—China,2022-2023

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China CDC weekly 2023年第49期5卷 1100-1106,I0003-I0005页

作者： Zhanwei Du Zeng.ang.Shao Xiao Zhang.Ruohan Chen Tianmu Chen y.an Bai Lin Wang.Eric H.y.lau Benjamin J.Cowling WHO Collaborating.Center for Infectious Disease Epidemiolog. and Control School of Public HealthLKS Faculty of MedicineThe University of Hong KongHong Kong Special Administrative RegionChina Laboratory.of Data Discovery.for Health Limited Hong Kong Science and Technology ParkHong Kong Special Administrative RegionChina State Key.Laboratory.of Molecular Vaccinolog. and Molecular Diag.ostics School of Public HealthXiamen UniversityXiamen CityFujian ProvinceChina Department of g.netics University of CambridgeCambridgeUK Institute for Health Transformation&School of Health&Social Development Deakin UniversityMelbourneAustralia

Backg.ound:Seasonal influenza resurg.d in China in February.2023,causing.a larg. number of *** influenza epidemics occurred across China during.the coronavirus disease 2019(COVID-19)pandemic,the relaxation of COVID-19 containment measures in December 2022 may.have contributed to the spread of acute respiratory.infections in winter 2022/***:Using.a mathematical model incorporating.influenza activity.as measured by.influenza-like illness(ILI)data for northern and southern reg.ons of China,we reconstructed the seasonal influenza incidence from October 2015 to September 2019 before the COVID-19 *** this trained model,we predicted influenza activities in northern and southern China from March to September ***:We estimated the effective reproduction number Re as 1.08[95%confidence interval(CI):0.51,1.65]in northern China and 1.10(95%CI:0.55,1.67)in southern China at the start of the 2022-2023 influenza *** estimated the infection attack rate of this influenza wave as 18.51%(95%CI:0.00%,37.78%)in northern China and 28.30%(95%CI:14.77%,41.82%)in southern ***:The 2023 spring.wave of seasonal influenza in China spread until July.2023 and infected a substantial number of people.

关键词： winter relaxation measures

佛教唯识宗“对象”概念初探:以《成唯识论》为据

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上海大学学报（社会科学版） 2007年第4期14卷 83-88页

作者：刘宇光复旦大学哲学学院上海200433

《成唯识论》对所缘的特殊诠释不单在于提出亲、疏之分,且进一步把疏所缘依其来源再分为同聚、异聚,即自己与他人两类。《成唯识论》借助此一诠释解决表像论的单边认知所遇上的符应难题,即以个体众生之间依名言而起的相向互动,为建立共... 详细信息

《成唯识论》对所缘的特殊诠释不单在于提出亲、疏之分,且进一步把疏所缘依其来源再分为同聚、异聚,即自己与他人两类。《成唯识论》借助此一诠释解决表像论的单边认知所遇上的符应难题,即以个体众生之间依名言而起的相向互动,为建立共识提供可能性,从而把问题的性质由个体的、单向的静态认知如何符应物质性对象世界转换为个体之间相向地、动态地以名言主动地塑造或建构彼此的共识。

关键词：所缘言义相分玄奘《成唯识论》