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Detection of Contamination and Analysis of Vertical Transmission of BmNPV in Eggs and Moths of Bombyx mori

Open Journal of Genetics 2014年第5期4卷 370-377页

作者： claudia regina das Neves Saez Roxelle Ethienne Ferreira Munhoz Naiara Climas Pereira Thais Souto Bignotto Veronica Aureliana Fassina Graziele Milani Pessini Laura Beatriz Garay Lucineia de Fatima Chasko Ribeiro Rose Meire Costa Brancalhao Maria Aparecida Fernandez Departamento de Biotecnologia Genetica e Biologia CelularUniversidade Estadual de MaringaMaringaBrasil Seda y Fibras HernandariasParaguay Universidade Estadual do Oeste do Parana CascavelBrasil

This study reports the molecular detection of Bombyx mori nucleopolyhedrovirus (BmNPV) in silkworm strains of the Universidade Estadual de Maringá Brazilian Germplasm Bank (UBGB). DNA extraction was carried out by using six Bombyx mori female moths of each strain, followed by PCR amplification. A pair of primers was designed based on a specific sequence of the baculovirus genome related to the BmNPV ORF 14. Another pair of primers was used to amplify the silkworm Actin A3 gene segment, which was used as positive control. Twenty gene pools were analyzed, and fifteen revealed a fragment of 443 base pairs (bp), which indicated the presence of the BmNPV. The frequency of contaminated moths was as following: 100% for silkworm strains M18-2, M12-2 and J1;83% for C25, C75 and C24 strains;66% for KR01;50% for M11-A;33% for AS3, B106, M8 and M11 and 16% for C211, E8 and Hindu strains. These are promising results for the identification of contaminated B. mori moths by BmNPV, which may prevent virus proliferation in subsequent generations. We also analyzed DNA samples extracted from B. mori eggs, but the results were not conclusive regarding the detection of the fragments of the expected size (443 bp). The difficulty in detecting BmNPV contamination in B. mori eggs may be due to the low concentration of virus in samples.

关键词： Silkworm Diseases Sericulture Viruses in Insects BmNPV Bombyx mori

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Evaluation of the toxic effect of insecticide chlorantraniliprole on the silkworm Bombyx mori(Lepidoptera:Bombycidae)

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Open Journal of Animal Sciences 2013年第4期3卷 343-353页

作者： Roxelle Ethienne Ferreira Munhoz Thais Souto Bignotto Naiara Climas Pereira claudia regina das Neves Saez Rafaela Bespalhuk Veronica Aureliana Fassina Graziele Milani Pessini Mayarha Patricia Dequigiovanni Baggio Lucineia de Fatima Chasko Ribeiro Rose Meire Costa Brancalhao Shunsuke Mizuno Willian Shigeaki Aita Maria Aparecida Fernandez Departamento de Biotecnologia Genetica e Biologia CelularUniversidade Estadual de MaringaMaringaBrasil Universidade Estadual do Oeste do Parana CascavelBrasil Fiacao de Seda Bratac S.A.Bastos Sao PauloBrasil

The silkworm Bombyx mori feeds exclusively on mulberry leaves and is highly sensitive to pesticides in general. Although mulberry plantations are free of agrochemicals, pesticide drift can occur. Chlorantraniliprole, a novel insecticide of the anthranilic diamides class, has been used to control pests in field crops. In this study, we investigated the biological effects of different concentrations of chlorantraniliprole on B. mori silkworm commercial Brazilian hybrids. To evaluate the toxicity of chlorantraniliprole, bioassays were carried out and data on the lethal concentrations, symptomatology, morphology and variables of silk production were collected. Results indicated that B. mori is extremely sensitive to chlorantraniliprole, even in low concentrations. The highest silkworm mortality rates were observed in the two highest chlorantraniliprole concentrations, 0.2 and 0.1 ppm. Although lower chlorantraniliprole concentrations did not cause death of all the silkworm larvae, various symptoms of toxicity were observed: feeding cessation, regurgitation, late development and incomplete ecdysis. Such symptoms reflect the morphological changes we observed in the midgut epithelium, which affected nutrient uptake and metabolism, and even the production of cocoons. Exposed larvae also produced?thin-shelled cocoons, which constitutes a serious economic problem because this type of cocoon is not useful for the silk industry. The results provided herein confirm the toxicity of chlorantraniliprole in silkworm larvae. Therefore, we strongly suggest that, competent authorities of the National Health Surveillance Agency, in pesticide management should take measures to reduce or eliminate the use of chlorantraniliprole in areas nearest to silkworm cultivation.

关键词： Silkworm Pesticide Drift Mortality Symptomatology Chlorantraniliprole

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Risk of Upper Extremity Biomechanical Overload in Automotive Facility

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Biomedical and Environmental Sciences 2013年第1期26卷 70-75页

作者： Angela SANCINI Assunta CAPOZZELLA Tiziana CACIAR Francesco TOMEI Nadia NARDONE Barbara SCALA Maria FIASCHETTI Carlotta CETICA Lara SCIMITTO PierAgostino GIOFFRè Federica SINIBALDI claudia Di PASTENA Paola CORBOSIERO Maria Pia SCHIFANO Gianfranco TOMEI Manuela CIARROCCA University of Rome "Sapienza" Department of AnatomyHistologyMedical-Legal and OrthopedicsUnit of Occupational MedicineViale Regina Elena 33600161 RomeItaly University of Rome "Sapienza" Department of Psychiatric and Psychological SciencePiazzale Aldo Moro 500185 RomeItaly

Objective To assess the risk factors for upper extremity-work-related musculoskeletal disorders（UE-WMSD） on 13 prod ucti on lines in an airbag factory using the threshold limit values-American conference of industrial hygienists-hand activity level（TLV-ACGIH-HAL） method and introduce the ergonomic improvement to reduce the repe titi veness and the peak force（Pf）.Methods Professional exposure level on 13 production lines in a automobile factory was measured using the TLV-ACGIH-HAL method and a further risk was assessed according to the ergonomic *** The first assessment of 9 produc tion lines showed that the professional exposure level was above the TLV or HAL *** second assessment showed that the professional exposure level was below the AL limit on all production lines except 1,in which the professional exposure level was between TLV and *** The assessment of UE-WMSD-related risk can ide ntif y the riskiest emplacements and evaluate the reduc tion of risk in professional exposure through interventi ons of structuralorganizati onal type.

关键词： Repe titi ve acti vity Work-related musculoskeletal disorders Assessment of work-related risk Threshold limit values-American conference of industrial hygienists Hand activity level.

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Study of Effects of Photodynamic Therapy(PDT),in Scar-Induced Skin Wounds in Rats Wistar:The New Clinical Perspective for Ulcers

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Modern Research in Inflammation 2017年第1期6卷 1-8页

作者： Marcus Vinicius de Mello Pinto Aline Ronis Sampaio Reggiani Vilela Goncalves Carlos Eduardo Tenorio da Veiga Lamara Laguardia Valente Rocha Daniel Almeida da Costa Lina claudia Pereira Lopes Rafael Batista Ferreira Pamela Mara Cainelli Valim Kellen regina Rodrigues Chaveiro Elias Sobreira Sathler Richard Raphael Borges Tavares Vieira Isabela Nardoni Bernardes Medical School of the University of Buenos Aires Bueinos AiresArgentina The Cellurare Institute Center of Laser Treatment and Cell Therapy PetropolisBrazil Department of Cellular and Structural Biology of Federal University of Vicosa VicosaBrazil Clinical Hospital of Correas Rio de JaneiroBrazil The Medical School at the Health Sciences Institute of the University Center of Caratinga CaratingaBrazil The Medical School at the Center of Higher Education of Valenca ValencaBrazil Luiz Gioseffi Jannuzzi School Hospital of the Dom Andre Arcoverde Foundation ValencaBrazil

Photodynamic therapy today is becoming an important role in the healing of lacerated tissues, since it has therapeutic resources capable of accelerating this process. One treatment option is the clinical phototherapy, and Photodynamic Therapy (PDT) is being widely used. This study aims to evaluate the effect of PDT on the healing of skin wounds in rats. We used a sample of 39 male rats Wistar divided into three groups, a control, a PDT-treated green and red with the last PDT. After 24 hours before the surgical incisions, PDT was used in both groups for 6 minutes and was evaluated in histological level, the inflammatory reaction and the repair process. The results showed that the granulation tissue was more developed in the irradiated group than in the control group and the amount of chronic inflammatory cells (monocytes, macrophages, lymphocytes and plasma cells) predominated with green phototherapy. The epithelialization in the wound margins and scarring with better quality occurred with red PDT (640 nm), which the higher deposition was of collagen. However, phototherapy not collimated of 640 nm (red) resulted in better anti-inflammatory effects. Given these answers with phototherapy not collimated green (525 nm) and red (640 nm), we conclude that the use of these wavelengths is possible with benefits, mainly with red LED.

关键词： Healing Photodynamic Therapy(PDT) Light Emitting Diodes(LEDs) Ulcer

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Pre-formulation Study for Preparation of Mucoadhesive Buccal Tablets Containing Nystatin and Cashew Gum by Direct Compression

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Journal of Physical Science and Application 2019年第1期9卷 1-14页

作者： Ana Paula de SáPinto Abrahao Magalhaes Flávia Almada do Carmo claudia regina Elias Mansur Institute of Macromolecules Federal University of Rio de JaneiroRio de Janeiro 21941-598Brazil Faculty of Pharmacy Federal University of Rio de JaneiroRio de Janeiro 21941-599Brazil Program of Materials Engineering and Metallurgy Technology CenterAlberto Luiz Institute of CoimbraPost-Graduation and Engineering Research-COPPEFederal University of Rio de JaneiroRio de Janeiro 21941-594Brazil

Cashew gum is a branched chain heteropolysaccharide extracted from the cashew tree (Anacardium occidentale L.).Purified cashew gum (PCG) is free of plant contaminants and is highly *** studies have indicated this polymer can be relevant in the pharmaceutical industry for production of ***,our research group reported that PCG can be used as a diluent for tablets produced by direct *** (Nys) is the drug of first choice for treatment of oral candidiasis,in the form of a *** treatment consists of up to six daily doses of a suspension of nys at 500,000 IU,causing low therapeutic adhesion by *** objective of this study was to investigate the behavior of PCG together with nys and other excipients (flavoring agents and lubricating agent) for future manufacture of mucoadhesive buccal tablets by direct *** that purpose,we performed pre-formulation tests (FTIR,TGA,XRD,solubility,pH,granulometry,swelling degree and powder flow) with physical mixtures of the drug and *** results were excellent,demonstrating that PCG is a polymer with potential for this type of application.

关键词： Purified cashew gum nystatin mucoadhesive buccal tablets

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Long-term survival of patients with stage Ⅱ and Ⅲgastric cancer who underwent gastrectomy with inadequate nodal assessment

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World Journal of Gastrointestinal Surgery 2021年第11期13卷 1463-1483页

作者： Jacopo Desiderio Andrea Sagnotta Irene Terrenato Eleonora Garofoli claudia Mosillo Stefano Trastulli Federica Arteritano Federico Tozzi Vito D'Andrea Yuman Fong Yanghee Woo Sergio Bracarda Amilcare Parisi Department of Digestive Surgery St.Mary’s HospitalTerni 05100Italy Department of Surgical Sciences Sapienza University of RomeRome 00161Italy Department of General Surgery and Surgical Oncology San Filippo Neri HospitalRome 00135Italy Biostatistics and Bioinformatic Unit Scientific DirectionIRCCS Regina Elena National Cancer InstituteRome 00144Italy Department of Medical Oncology St.Mary’s HospitalTerni 05100Italy Division of Surgical Oncology and Endocrine Surgery Mays Cancer CenterUniversity of Texas Health Science Center San AntonioSan AntonioTX 78229United States Division of Surgical Oncology Department of SurgeryCity of Hope National Medical CenterDuarteLA91010United States

BACKGROUND Gastric cancer is an aggressive disease with frequent lymph node(LN)*** NCCN recommends a D2 lymphadenectomy and the harvesting of at least 16 *** threshold has been the subject of great debate,not only for the extent of surgery but also for more appropriate *** reclassification of stage IIB through IIIC based on N3b nodal staging in the eighth edition of the American Joint Committee on Cancer(AJCC)staging system highlights the efforts to more accurately discriminate survival expectancy based on nodal ***,studies have suggested that pathologic assessment of 30 or more LNs improve prognostic accuracy and is required for proper staging of *** To evaluate the long-term survival of advanced gastric cancer patients who deviated from expected survival curves because of inadequate nodal *** Eligible patients were identified from the Surveillance,Epidemiology,and End Results *** with stage II-III gastric cancer were considered for *** groups were compared based on the number of analyzed *** were inadequate LN assessment(ILA,<16 LNs),adequate LN assessment(ALA,16-29 LNs),and optimal LN assessment(OLA,≥30 LNs).The main outcomes were overall survival(OS)and cancer-specific *** were analyzed by the Kaplan-Meier product-limit method,log-rank test,hazard risk,and Cox proportional univariate and multivariate *** score matching(PSM)was used to compare the ALA and OLA *** The analysis included 11607 *** had advanced T stages(T3=48%;T4=42%).The pathological AJCC stage distribution was IIA=22%,IIB=18%,IIIA=26%,IIIB=22%,and IIIC=12%.The overall sample divided by the study objective included ILA(50%),ALA(35%),and OLA(15%).Median OS was 24 mo for the ILA group,29 mo for the ALA group,and 34 mo for the OLA group(P<0.001).Univariate analysis showed that the ALA and OLA groups had better OS than the ILA group[ALA hazard ratio(HR)=0.84,95%co

关键词： Gastric Cancer Lymphadenectomy Gastrectomy Staging N stage Surveillance,Epidemiology,and End Results

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