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Effect of Protein Adsorption onto the Dissolution of Silicon-Substituted Hydroxyapatite

Journal of Encapsulation and Adsorption Sciences 2011年第4期1卷 72-79页

作者： claudia manuela botelho Roger Brooks Masanobu Kanitakahara Chikara Ohtsuki Serena Best Maria Ascensao Lopers Neil Rushton William Bonfield JoséDomingos Santos Institute for Biotechnology and Bioengineering Centre of Biological EngineeringUniversidade do MinhoCampus de GualtarBragaPortugal Graduate School of Materials Science Nara Institute of Science and TechnologyTakayamaIkomaNaraJapan Orthopaedic Research Unit Addenbrooke’s HospitalCambridgeUK Department of Materials Science and Metallurgy University of CambridgeCambridgeUK Tohoku University Graduate School of Environmental StudiesAramakiAobaAoba-kuSendaiJapan Nagoya University Department of Crystalline Materials ScienceGraduate School of EngineeringFuro-choChikusa-kuNagoyaJapan CEMUC Faculty of EngineeringDepartment of Metallurgical and Materials EngineeringUniversity of PortoPortoPortugal

Several authors have shown the beneficial role to incorporate silicon into hydroxyapatite lattice, although, the mechanism behind the enhanced bioactivity of this Si-hydroxyapatite (Si-HA) is poorly understood. The incorporation of Si into the HA lattice alters the surface charge of HA, leading to more negative values. Due to the importance of the surface properties on the interaction between biomaterials, physiological fluids, and the host tissue, it is important to further characterize the surface of Si-HA by determining its surface energy and wettability. Our results showed that the incorporation of Si increased the hydrophilicity of HA, leading to a higher interfacial tension. Another important property for osteointegration is the formation of an apatite layer. The dissolution of Si-HA in the presence of serum-free simulated body fluid (SBF) started at early time points and using atomic force microscopy (AFM) it was possible to observe the dissolution at the grain boundaries and grains, therefore an apatite layer was formed in a short period of time. As the dissolution-precipitation process is much more complex in vivo, we tried to mimic the initial stages of the in vivo reaction by incubating the Si-HA in serum-SBF. It was shown that the dissolution kinetics in serum-SBF was slower when compared to the dissolution in serum free-SBF. At the same time point, no significant dissolution features were observed or apatite layer was visualized. The phase imaging AFM indicated the presence of a layer on top of these materials that could be a proteinaceous layer, as XPS analysis detected an increase on the concentration of nitrogen on the surface of the samples incubated in the presence of proteins.

关键词： Biomaterials Silicon Surface Properties Proteins Dissolution

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Arabidopsis OBG-Like GTPase （AtOBGL）Is Localized in Chloroplasts and Has an Essential Function in Embryo Development

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Molecular Plant 2009年第6期2卷 1373-1383页

作者： Fatima Chigri claudia Sippel manuela Kolb Ute C. Vothknecht Center for Integrated Protein Science (Munich) Department of Biology LMU Munich D-81377 Munich Germany Department of Biology I Botany LMU Munich D-82152 Planegg-Martinsried Germany

OBG-like GTPases, a subfamily of P-loop GTPases, have divers and important functions in bacteria, including initiation of sporulation, DNA replication, and protein translation. Homologs of the Bacillus subtilis spo0B GTP-binding protein （OBG） can be found in plants and algae but their specific function in these organisms has not yet been elucidated. Here, it is shown that ATSG18570 encodes an Arabidopsis thaliana OBG-like protein （AtOBGL） that is localized in chlor- oplasts. In contrast to the bacterial members of this protein family, AtOBGL and other OBG-like proteins from green algae and plants possess an additional N-terminal domain, indicating functional adaptation. Disruption of the gene locus of ATOBGL by TDNA insertion resulted in an embryo-lethal phenotype and light microscopy using Normarski optics revealed that embryo maturation in the atobgl mutant is arrested at the late globular stage before development of a green embryo. Expression of 35S：：ATOBGL within the atobgl mutant background could rescue the mutant phenotype, confirming that embryo-lethality is caused by the loss of AtOBGL. Together, the data show that the bacterial-derived OBG-like GTPases have retained an essential role in chloroplasts of plants and algae. They furthermore corroborate the significance of chloroplast functions for embryo development -- an important stage within the Arabidopsis lifecycle.

关键词： GTPase OBG embryo development chloroplast.

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Improved sexual behavior in male rats treated with a Chinese herbal extract： hormonal and neuronal implications

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Asian Journal of Andrology 2008年第6期10卷 937-945页

作者： Paola Zanoli Augusta Benelli manuela Zavatti Marianna Rivasi claudia Baraldi Mario Baraldi Department of Biomedical Sciences Section of Pharmacology and National InterUniversity Consortium for the Study of Natural Active Principles University of Modena and Reggio Emilia Modena 41100 Italy

Aim： To investigate the influence of an extract obtained from five Chinese medicinal plants on sexual behavior of adult male rats. Methods： The extract was administered at doses of 30, 60 and 120 mg/kg by oral gavage, acutely （one time, 45 min before mating test） or subchronically （daily for 10 days） in sexually potent and sexually sluggish/impotent rats. Sexual behavior, serum levels of luteinizing hormone （LH） and testosterone （T） were evaluated in treated rats and compared with controls receiving vehicle. The effect of the extract on central dopaminergic neurotransmission was assessed in the nucleus accumbens using a microdialysis technique. Results： In sexually potent rats, both acute and subchronic treatment with the extract dosed at 30 and 60 mg/kg reduced mount latency and intromission latency. In sluggish/impotent rats, the acutely administered extract at the dose of 60 mg/kg shortened ejaculation latency, whereas subchronically administered at the doses of 30 and 60 mg/kg, reduced mount, intromission and ejaculation latencies, increasing also the percentage of mounting and ejaculating rats. The extract dosed at 60 mg/kg significantly increased LH and T following acute and subchronic administration and increased 3,4-dihydroxyphenylacetic acid levels in the nucleus accumbens, 30 min after the acute administration. Conclusion： The improvement in both appetitive and consummatory components of sexual behavior observed in male rats treated with the extract could be ascribed to increased serum T level in parallel with the activation of the central dopaminergic system.

关键词： sexual behavior rats testosterone luteinizing hormone microdialysis dopamine 3,4-dihydroxyphenylacetic acid

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UV Stability of Poly（Lactic Acid） Nanocomposites

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材料科学与工程（中英文B版） 2013年第2期3卷 75-83页

作者： Andreia Aratujo Gabriela Lema botelho manuela Silva Ana Vera Machado Chemistry Department University of Minho Campus de Gualtar Brag a 4710-057 Portugal Institute for Polymers and Composites (IPC)/I3N University of Minho Campus de Azur~m Guimar6es 4800-058 Portugal

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Risk of Upper Extremity Biomechanical Overload in Automotive Facility

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Biomedical and Environmental Sciences 2013年第1期26卷 70-75页

作者： Angela SANCINI Assunta CAPOZZELLA Tiziana CACIAR Francesco TOMEI Nadia NARDONE Barbara SCALA Maria FIASCHETTI Carlotta CETICA Lara SCIMITTO PierAgostino GIOFFRè Federica SINIBALDI claudia Di PASTENA Paola CORBOSIERO Maria Pia SCHIFANO Gianfranco TOMEI manuela CIARROCCA University of Rome "Sapienza" Department of AnatomyHistologyMedical-Legal and OrthopedicsUnit of Occupational MedicineViale Regina Elena 33600161 RomeItaly University of Rome "Sapienza" Department of Psychiatric and Psychological SciencePiazzale Aldo Moro 500185 RomeItaly

Objective To assess the risk factors for upper extremity-work-related musculoskeletal disorders（UE-WMSD） on 13 prod ucti on lines in an airbag factory using the threshold limit values-American conference of industrial hygienists-hand activity level（TLV-ACGIH-HAL） method and introduce the ergonomic improvement to reduce the repe titi veness and the peak force（Pf）.Methods Professional exposure level on 13 production lines in a automobile factory was measured using the TLV-ACGIH-HAL method and a further risk was assessed according to the ergonomic *** The first assessment of 9 produc tion lines showed that the professional exposure level was above the TLV or HAL *** second assessment showed that the professional exposure level was below the AL limit on all production lines except 1,in which the professional exposure level was between TLV and *** The assessment of UE-WMSD-related risk can ide ntif y the riskiest emplacements and evaluate the reduc tion of risk in professional exposure through interventi ons of structuralorganizati onal type.

关键词： Repe titi ve acti vity Work-related musculoskeletal disorders Assessment of work-related risk Threshold limit values-American conference of industrial hygienists Hand activity level.

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Assessment of the bioaccessibility and bioavailability prediction of omega 3 and conjugated fatty acids by in vitro standardized digestion model(INFOGEST)and cell model

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Food Bioscience 2023年第3期53卷 1678-1691页

作者： Ana Sofia Salsinha Sara A.Cunha manuela Machado Luís Miguel Rodríguez-Alcalá João B.Relvas manuela Pintado Universidade Católica Portuguesa CBQF-Centro de Biotecnologia e Química Fina–Laboratório AssociadoEscola Superior de BiotecnologiaRua de Diogo Botelho13274169-005PortoPortugal Instituto de Investigação e Inovação Em Saúde and Instituto de Biologia Molecular e Celular(IBMC) Universidade Do Porto–Rua Alfredo Allen2084200-135PortoPortugal Departmento de Biomedicina Faculdade de Medicina da Universidade do Porto(FMUP)4200-319 PortoPortugal

Omega 3 EPA and DHA are polyunsaturated fatty acids with relevant health *** linoleic and linolenic acids are known for their anti-carcinogenic effect,anti-inflammatory properties and body weight *** achieve therapeutical doses,high amounts of these fatty acids’food sources must be ***,the intake of enriched oils with a high concentration of these fatty acids is often *** several factors influence their ***,by using the INFOGEST static in vitro protocol of gastrointestinal tract digestion it was studied the bioaccessibility of these fatty acids in different matrixes:Pomegranate and Fish oil and omega 3,CLA and CLNA soft-gel enriched *** digestion,the Recovery Index for the major bioactive PUFAs are very low:Pomegranate oil is 2%,Fish oil 11-13%,CLNA 17%,CLA 6%and Omega 3 capsules 3%.Higher initial concentrations of these PUFAs seem to be related to higher degrees of *** Pomegranate oil,CLNA and Omega 3 capsules,the digestion process negatively influenced the antioxidant *** opposite was verified for the Fish oil and CLA ***,bioaccessibility studies of similar matrixes are very scarce and intestinal permeability is absent in most of the *** permeability studies were performed using a Caco-2/HT29-MTX co-culture:there is significative incorporation of the bioactive fatty acids into the intestinal cells,which may affect their permeability ***,most fatty acids remain in the non-bioaccessible fraction which may be relevant when designing oral routes of administration and in gut microbiota modulation.

关键词： Fish oil Pomegranate oil INFOGEST digestion Method Bioaccessibility Intestinal permeability Bioactive fatty acids

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Impact of simulated in vitro gastrointestinal digestion on bioactive compounds,bioactivity and cytotoxicity of melon (Cucumis melo L. inodorus) peel juice powder

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Food Bioscience 2022年第3期47卷 1358-1367页

作者： Ricardo Gómez-García Ana A.Vilas-Boas manuela Machado Débora A.Campos Cristóbal N.Aguilar Ana R.Madureira manuela Pintado Universidade Católica Portuguesa CBQF-Centro de Biotecnologia e Química Fina – Laboratório AssociadoEscola Superior de BiotecnologiaRua Diogo Botelho 13274169-005PortoPortugal BBG-DIA.Bioprocesses and Bioproducts Group.Food Research Department School of ChemistryAutonomous University of CoahuilaSaltilloCoahuilaMexico

The objectives of this research work were to evaluate the effect of in vitro gastrointestinal digestion (GIT) on melon peel juice (MPJ) powder from fruit processing industry by-products,considering (i) the recovery and accessibility indexes,(ii) the changes on antioxidant activity,and (iii) the prebiotic *** exposition to GIT conditions a decrease on the total phenolic content (TPC = 65.31%) and antioxidant activity by ABTS = 39.77% and DPPH = 45.91% were ***,these both parameters exhibited stable accessibility,accounting with 81.89%,76.55%,and 54.07% for TPC,ABTS and DPPH,*** gastrointestinal digestion,the non-absorbed fraction exhibited a positive impact on the growth of Bifidobacterium and Lactobacillus strains,possibly associated with the high content of simple sugar (glucose and fructose).This fraction also showed to be safe on Caco-2 intestinal *** findings suggest that MPJ might be used as a potential food functional ingredient.

关键词： Melon by-products Functional ingredients Phenolic antioxidants Simulated digestion Prebiotic effect

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Functional properties of peptides obtained from whey proteins by ficin extract hydrolysis

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Food Bioscience 2022年第3期47卷 918-927页

作者： Afaf Kheroufi María E.Brassesco Débora A.Campos Halima Boughellout manuela E.Pintado Laboratoire de Génie Agro-alimentaire équipe Génie des Procédés AlimentairesBiodiversité et Agro environnementInstitut de la Nutritionde l’Alimentation et des Technologies Agroalimentaires INATAAUniversité Frères Mentouri Constantine 1BP 325 Route de Ain El BeyConstantineAlgeria CBQF – Centro de Biotecnologia e Química Fina – Laboratório Associado Escola Superior de BiotecnologiaUniversidade Católica Portuguesa/PortoRua de Diogo Botelho 13274169-005PortoPortugal

Enzymatic whey proteins concentrates (WPC) hydrolysis under optimal conditions of ficin crude extract (FCE) in two ratios E/S (0.5 and 1%) was studied,assessing the impact of hydrolysis on WPC by determination degree of hydrolysis (DH),Fast proteins liquid chromatography (FPLC),Tricine SDS-PAGE,antioxidant activity at sampling time beside,and technological properties (solubility,emulsifying activity index (EAI) and foaming activity (FA)) at the best condition (maximum DH and antioxidant activity) other *** showed,in the first 30 min,DH reached 22.76% at E/S (1%) with a total degradation of α-lactalbumin (α-La) and almost of β-lactoglobulin (β-Lg).A significant increase in antioxidant activity and solubility was observed,while not for EAI,and *** results indicate for the first time that β-Lg is susceptible to hydrolyse by FCE that rarely found for other *** hydrolysates showed very relevant performance,and suggest that could be appropriate production of functional ingredients.

关键词： Whey proteins Ficin Hydrolysis Antioxidant Solubility

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Novel avocado oil-functionalized yogurt with anti-obesity potential:Technological and nutraceutical perspectives

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Food Bioscience 2022年第6期50卷 161-170页

作者： manuela Machado Sérgio Sousa Pilar Morais Arménio Miranda Luís M.Rodriguez-Alcalá Ana Maria Gomes manuela Pintado Universidade Católica Portuguesa CBQF Centro de Biotecnologia e Química Fina-Laboratório AssociadoEscola Superior de BiotecnologiaRua Diogo Botelho 13274169-005PortoPortugal Frulact S.ARua do Outeiro589Gemunde4475-150MaiaPortugal

Studies on bioactive lipids have been increasing in the last years due to their proven health *** purpose of this research work was to develop a yogurt with bioactive lipids with anti-obesity *** this purpose,yogurts were produced with avocado oil in free form or encapsulated as a *** presence of avocado oil enhanced the nutritional quality of yogurts through a reduction(more than 75%)of their atherogenic and thrombogenic *** high nutritional value was particularly evident in samples with bigel,as these yogurts presented eight-fold higher levels of total fatty acids than the yogurts with avocado oil in free *** addition,yogurts with avocado bigel presented better stability throughout the gastrointestinal tract-results showed a higher recovery percentage of oleic acid(47.2%)when compared to 24%in the yogurts with avocado in free *** results from the in vitro studies demonstrated that these functional yogurts can increase the lipolytic rate in adipocytes,translated by an increase of ca.20%in glycerol release in yogurts with ***,the hepatic lipid accumulation in cells subjected to steatosis induced by chloroquine was reduced by 35%in cells treated with yogurt digested *** results showed the potential of these functional yogurts to modulate obesity-related metabolism.

关键词： Lipid metabolism Obesity Dairy products Functional foods

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Auditory Health and Risk Factors in Rural Workers Exposed to Pesticides in Piedade de Caratinga County-Minas Gerais

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Journal of Biosciences and Medicines 2017年第7期5卷 37-55页

作者： Paula Critina da Silva botelho Valdênia Gomes Rocha Tamires Ribeiro Dutra Júlio César Alves Feitosa Ricardo Campos de Assis Thiago Sande Miguel Nayrton Kalys Cruz dos Anjos Daniel Almeida da Costa Lina claudia Pereira Lopes Lamara Laguardia Valente Rocha Department of Nursing University Center of Caratinga Caratinga Brazil Graduating in medicine University Center of Caratinga Caratinga Brazil Graduating in Psychology University Center of Caratinga Caratinga Brazil The Medical School at the Center of Higher Education of Valenca Valenca Brazil Department of Medicine Graduate Program in Medicine Faculty of Medicine of Valencia Brazil Speech Therapist The Medical School at the Center of Higher Education of Valenca Valenca Brazil Department of Morphology University Center of Caratinga Caratinga Brazil

In Brazil, the amount of pesticides consumed doubled in the last 10 years. Thus, in 2008 it assumed the lead position in the world. However, its indiscriminate application in agriculture without the use of the necessary care has contributed to environmental degradation and increased occupational exposures, making it a serious health problem in the country. The recent study looked to evaluate the auditory health and risk factors in rural workers exposed to pesticides in Piedade de Caratinga County—MG, and chart their socioeconomic profile and lifestyle while identifying the types of crops and the use and management of pesticides. Developed, then, a descriptive and exploratory study involving 23 men, rural workers, exposed to pesticides, with no history of exposure to noise, with changes in meatoscopy and aged ≤50 years of age. The results show the sample consisted of 23 men with a mean age of 36.7 ± 9.3, whom have low education, with a monthly income of 1 minimum wage, working in the fields for over 15 years. There was a predominance of horticulture using organophosphate pesticides and pyrethroids. Part of the sample shows auditory loss, the most common sensorineural conductive loss. It is essential that the population exposed to this type of risk is informed and receives guidance from educational campaigns developed by the multidisciplinary team of basic health units.

关键词： Auditory Health Pesticides Audiometry and Rural Workers

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